Abstract：
:Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This released secretory component was identical with free secretory component with respect to electrophoretic migration, isoelectric focusing point, and molecular weight, but lacked some antigenic determinants. The amino acid composition and the N-terminal sequence of canine free secretory component was similar to that reported for the cow.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Thompson RE,Reynolds HY,Waxdal MJdoi
10.1021/bi00684a010subject
Has Abstractpub_date
1975-07-01 00:00:00pages
2853-60issue
13eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The positions of single amino acids in the interhelical loop regions and the C-terminal tail of bacteriorhodopsin (bR) were investigated by X-ray diffraction using site-directed heavy-atom labeling. Since wild-type bR does not contain any cysteines, appropriate cysteine mutants were produced with a unique sulfhydryl g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971735s
更新日期：1998-07-21 00:00:00
abstract：:Drug efflux and enzymatic drug degradation are two cellular mechanisms that contribute to drug resistance in many cancers. Herein, we report the synthesis and in vitro activity of a pro-immunostimulant that exploits both processes in tandem to selectively confer cancer-mediated immunogenicity. We demonstrate that an i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00095
更新日期：2018-04-17 00:00:00
abstract：:Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962274y
更新日期：1997-04-08 00:00:00
abstract：:Disruption of the zinc-thiolate center at the active site of yeast alcohol dehydrogenase results in inactivation and zinc release. Measurements of activity, zinc release, and thiol/thiolate oxidation were used to assess the effects of biologically relevant oxidants on alcohol dehydrogenase. Alcohol dehydrogenase was i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00011a008
更新日期：1995-03-21 00:00:00
abstract：:One of the distinctive features of eubacterial retinal-based proton pumps, proteorhodopsins, xanthorhodopsin, and others, is hydrogen bonding of the key aspartate residue, the counterion to the retinal Schiff base, to a histidine. We describe properties of the recently found eubacterium proton pump from Exiguobacteriu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300409m
更新日期：2012-07-24 00:00:00
abstract：:Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952808f
更新日期：1996-03-26 00:00:00
abstract：:It had been previously reported that aromatic amines were not substrates for the bacterial quinoprotein methylamine dehydrogenase. In this study, benzylamine-dependent activity was also not observed in the steady-state assay of this enzyme with the artificial electron acceptor phenazine ethosulfate (PES). Benzylamines...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00128a012
更新日期：1992-04-07 00:00:00
abstract：:We have characterized a membrane protein containing residues P688-T762 of the integrin beta3 subunit, encompassing its transmembrane and cytoplasmic domains, by nuclear magnetic resonance spectroscopy. Under conditions in which it is monomeric in dodecylphosphocholine micelles, the protein consists mainly of alpha-hel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026822l
更新日期：2002-12-31 00:00:00
abstract：:Interaction of a mitochondria-specific anionic phospholipid, cardiolipin (CL), with an intermembrane protein, cytochrome c (cyt c), yields a peroxidase complex. During apoptosis, the complex induces accumulation of CL oxidation products that are essential for detachment of cyt c from the mitochondrial membrane, induct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801507s
更新日期：2008-12-23 00:00:00
abstract：:Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00300a016
更新日期：1984-02-28 00:00:00
abstract：:We describe a reconstitution of light-activated vertebrate photoreceptor GTPase and a purification of the GTP-binding protein (G protein), which is a component of the GTPase and modulates the light-activated phosphodiesterase (PDE) enzyme system. Rod outer segments (ROS) of bull frogs were treated with ethylenediamine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00528a003
更新日期：1981-12-08 00:00:00
abstract：:Notwithstanding the relevance of their biological function, slow motions in proteins, beyond the microsecond range, are still poorly understood and often elusive. We propose that acrylamide quenching of Trp phosphorescence of deeply buried residues, when extended over the entire accessible range of lifetime measuremen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9009659
更新日期：2009-08-11 00:00:00
abstract：:The pathway for utilization of pyridine nucleotide derived reducing equivalents in the cytochrome P-450 monooxygenase systems has three major branch points. The first is a partitioning between autoxidation of a ferrous, oxygenated heme adduct and input of the second reducing equivalent required for monooxygenase stoic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00094a009
更新日期：1993-11-02 00:00:00
abstract：:Proteolytic degradation of glutamine synthetase (GS) in Escherichia coli is known to follow "marking" by oxidative modification. At an early stage of the degradative pathway, oxidation of His 269 and Arg 344 abolishes GS enzymatic activity. We propose a mechanism for the early stage of oxidative inactivation of GS on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a022
更新日期：1993-08-10 00:00:00
abstract：:The effects of ions on the interaction of lac repressor protein and operator DNA have been studied by the membrane filter technique. The equilibrium association constant was determined as a function of monovalent and divalent cation concentrations, anions, and pH. The binding of repressor and operator is extremely sen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00516a027
更新日期：1981-06-23 00:00:00
abstract：:Small molecule allostery modifies protein function but is not easily discovered. We introduce mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS), a method for identifying physiologically relevant, low-affinity metabolite-protein interactions using unmodified pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201313s
更新日期：2012-01-10 00:00:00
abstract：:The expression of rusticyanin in Escherichia coli and a number of mutants for Ser86 is reported. Mutations of Ser86 to Asn, Asp, Gln, and Leu were undertaken as this is an Asn residue in other structurally characterized cupredoxins, and it has been suggested that this may be partly responsible for the high redox poten...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980960m
更新日期：1998-08-18 00:00:00
abstract：:Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to increased protein activity but also to higher stability, especially...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2016537
更新日期：2012-02-07 00:00:00
abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00277a038
更新日期：1983-04-12 00:00:00
abstract：:Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200008b
更新日期：2011-06-28 00:00:00
abstract：:Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00831
更新日期：2015-09-08 00:00:00
abstract：:Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0026324
更新日期：2001-02-27 00:00:00
abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) was rapidly inactivated by micromolar concentrations of ferrous sulfate in the presence of ascorbate at pH 7.4. Omitting ascorbate or replacing the Fe2+ with Mn2+ or Mg2+ gives no inactivation. Mn2+, Mg2+, or Co2+ at 100-fold molar excess over Fe2+ offered comple...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970574p
更新日期：1997-12-09 00:00:00
abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00469a004
更新日期：1990-05-01 00:00:00
abstract：:The molecular organization of sterols in liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at 37 degrees C is examined by utilizing the fluorescent analogue of cholesterol cholesta-5,7,9-trien-3 beta-ol (cholestatrienol). (1) Cholestatrienol is shown to be indistinguishable from native cholesterol i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00456a027
更新日期：1990-01-30 00:00:00
abstract：:RNA:pseudouridine synthetase (Pus1) from Saccharomyces cerevisiae is a multisite specific enzyme that catalyzes the formation of pseudouridine at positions 34 and 36 of intron-containing precursor tRNAIle and at positions 27 and/or 28 of several yeast tRNAs. In this paper we demonstrate that the purified recombinant P...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972671o
更新日期：1998-05-19 00:00:00
abstract：:Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300542g
更新日期：2012-09-04 00:00:00
abstract：:The truncated hemoglobins from Bacillus subtilis (Bs-trHb) and Thermobifida fusca (Tf-trHb) have been shown to form high-affinity complexes with hydrogen sulfide in their ferric state. The recombinant proteins, as extracted from Escherichia coli cells after overexpression, are indeed partially saturated with sulfide, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901671d
更新日期：2010-03-16 00:00:00
abstract：:Site-directed mutagenesis, electron microscopy, and X-ray crystallography were used to probe the structural basis of annexin IV-induced membrane aggregation and the inhibition of this property by protein kinase C phosphorylation. Site-directed mutants that either mimic (Thr6Asp, T6D) or prevent (Thr6Ala, T6A) phosphor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002507s
更新日期：2001-04-03 00:00:00
abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973162p
更新日期：1998-04-28 00:00:00
