Abstract:
:Site-directed mutagenesis, electron microscopy, and X-ray crystallography were used to probe the structural basis of annexin IV-induced membrane aggregation and the inhibition of this property by protein kinase C phosphorylation. Site-directed mutants that either mimic (Thr6Asp, T6D) or prevent (Thr6Ala, T6A) phosphorylation of threonine 6 were produced for these studies and compared with wild-type annexin IV. In vitro assays showed that unmodified wild-type annexin IV and the T6A mutant, but not PKC-phosphorylated wild-type or the T6D mutant, promote vesicle aggregation. Electron crystallographic data of wild-type and T6D annexin IV revealed that, similar to annexin V, the annexin IV proteins form 2D trimer-based ordered arrays on phospholipid monolayers. Cryo-electron microscopic images of junctions formed between lipid vesicles in the presence of wild-type annexin IV indicated a separation distance corresponding to the thickness of two layers of membrane-bound annexin IV. In this orientation, a single layer of WT annexin IV, attached to the outer leaflet of one vesicle, would undergo face-to-face self-association with the annexin layer of a second vesicle. The 2.0-A resolution crystal structure of the T6D mutant showed that the mutation causes release of the N-terminal tail from the protein core. This change would preclude the face-to-face annexin self-association required to aggregate vesicles. The data suggest that reversible complex formation through phosphorylation and dephosphorylation could occur in vivo and play a role in the regulation of vesicle trafficking following changes in physiological states.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kaetzel MA,Mo YD,Mealy TR,Campos B,Bergsma-Schutter W,Brisson A,Dedman JR,Seaton BAdoi
10.1021/bi002507ssubject
Has Abstractpub_date
2001-04-03 00:00:00pages
4192-9issue
13eissn
0006-2960issn
1520-4995pii
bi002507sjournal_volume
40pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have stu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a040
更新日期:1989-05-30 00:00:00
abstract::Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that phosphorylates and inactivates eEF-2 and that can regulate the rate of protein synthesis at the elongation stage. Here we report that a slight decrease in pH, within the range observed in vivo, leads to a dramatic activation of eE...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026494p
更新日期:2002-11-12 00:00:00
abstract::Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700247y
更新日期:2007-06-19 00:00:00
abstract::Pokeweed antiviral protein (PAP) is a ribosome inactivating protein (RIP) that depurinates the sarcin/ricin loop (SRL) of rRNA, inhibiting protein synthesis. PAP depurinates viral RNA, and in doing so, lowers the infectivity of many plant viruses. The mechanism by which PAP accesses uncapped viral RNA is not known, im...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00598
更新日期:2017-11-14 00:00:00
abstract::In this study, we investigated the role of ubiquitination in the disposition of the inactivated O6-methylguanine-DNA methyltransferase (MGMT) protein in human (HT-29 and CEM) and murine (ts85) tumor cells. Using a combination of immunoprecipitation and immunoblotting techniques with antibodies against ubiquitin and MG...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9518205
更新日期:1996-01-30 00:00:00
abstract::To describe the time course of cellular systems, we integrate ideas from thermodynamics and information theory to discuss the work needed to change the state of the cell. The biological example analyzed is experimental microarray transcription level oscillations of yeast in the different phases as characterized by oxy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3016982
更新日期:2013-03-05 00:00:00
abstract::High-resolution, solid-state 15N NMR has been used to study the chemical shift anisotropies of the Schiff bases in bacteriorhodopsin (bR) and in an extensive series of model compounds. Using slow-spinning techniques, we are able to obtain sufficient rotational sideband intensity to determine the full 15N chemical shif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00434a033
更新日期:1989-04-18 00:00:00
abstract::Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone hydrogen-bonding and side-chain hydrophobic contacts in the substrate and determining each substrate's kinetic constants. The observed specificity energies [defined as d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00219a033
更新日期:1991-02-05 00:00:00
abstract::Pterin-free inducible nitric oxide synthase (iNOS) was reconstituted with tetrahydrobiopterin (H(4)B) or tetrahydrobiopterin analogues (5-methyl-H(4)B and 4-amino-H(4)B), and the ability of bound 5-methyl-H(4)B and 4-amino-H(4)B to support catalysis by either full-length iNOS (FLiNOS) or the isolated heme domain (HDiN...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035491p
更新日期:2003-11-18 00:00:00
abstract::Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800806d
更新日期:2008-08-19 00:00:00
abstract::The relationship between steroid binding and protein subunit interactions of rabbit sex steroid binding protein (rSBP) has been studied by steady-state and time-resolved fluorescence spectroscopy. The high-affinity (Ka approximately 10(8) M-1 at 4 degrees C), fluorescent estrogen d-1,3,5(10),6,8-estrapentaene-3,17 bet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00492a005
更新日期:1990-10-09 00:00:00
abstract::The Rhodnius prolixus aggregation inhibitor 1 (RPAI-1) is a novel blood-sucking salivary molecule that binds to ADP and attenuates platelet aggregation. In this report, we determine the binding constants and specificity of RPAI-1 for adenine nucleotides and its functional significance. By the Hummel-Dreyer method of e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011015s
更新日期:2002-03-19 00:00:00
abstract::Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961200j
更新日期:1996-11-26 00:00:00
abstract::The pre-steady-state kinetics of the microtubule-kinesin ATPase were investigated by chemical-quench flow methods using the Drosophila kinesin motor domain (K401) expressed in Escherichia coli [Gilbert, S. P., & Johnson, K. A. (1993) Biochemistry 32, 4677-4684]. The results define a minimal mechanism: M.K + ATP in equ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00173a044
更新日期:1994-02-22 00:00:00
abstract::Chemical modification of some trifunctional amino acid residues in toxins I, II, and III of the scorpion Androctonus australis Hector have been performed. The results indicate: (1) Reduction and methylation of one disulfide bridge destroy toxic activity of toxin II. (2) The only tryptophan residue of toxin II (positio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00656a002
更新日期:1976-06-01 00:00:00
abstract::We have investigated in detail the cooperativity which we had previously observed in the pH titration profiles of the histidine residues of rabbit tropomyosin [Edwards, B. F. P., & Sykes, B. D. (1978) Biochemistry 17, 684]. Nonpolymerizing tropomyosin was prepared by carboxypeptidase digestion, and the titration profi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00517a037
更新日期:1981-07-07 00:00:00
abstract::The phosphoramidate of CCdAp and puromycin (CCdApPuro) is a potent inhibitor of ribosomal peptidyl transferase, as assayed by the fragment reaction. Inhibition is competitive at the ribosomal A-site. CCdApPuro protects P-site-associated bases in the peptidyl transferase loop region of 23S rRNA from carbodiimide modifi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00002a001
更新日期:1995-01-17 00:00:00
abstract::The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700883g
更新日期:2007-12-04 00:00:00
abstract::Secretory carrier membrane protein 2 (SCAMP2) functions in late steps of membrane fusion in calcium-dependent granule exocytosis. A basic/hydrophobic peptide segment within SCAMP2 (SCAMP2 E: CWYRPIYKAFR) has been implicated in this function and shown to bind and sequester phosphatidylinositol 4,5-bisphosphate [PI(4,5)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701121j
更新日期:2007-09-25 00:00:00
abstract::A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00202a024
更新日期:1994-09-13 00:00:00
abstract::We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00112a007
更新日期:1991-12-03 00:00:00
abstract::Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a048
更新日期:1989-01-10 00:00:00
abstract::Urea lesions are formed in DNA because of free radical damage of the thymine base, and their occurrence in DNA blocks DNA polymerases, which has deleterious consequences. Recently, it has been shown that urea is capable of forming hydrogen bonding and stacking interactions with nucleobases, which are responsible for t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00309
更新日期:2016-10-11 00:00:00
abstract::The effects of various compounds such as the transport substrate ADP and the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA) on the labeling of cysteine residues in the ADP/ATP carrier of bovine heart submitochondrial particles by the SH reagent eosin-5-maleimide (EMA) were studied. Of the f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00198a019
更新日期:1994-08-16 00:00:00
abstract::ArsR (or ArsR/SmtB) family metalloregulatory homodimeric repressors collectively respond to a wide range of metal ion inducers in regulating homeostasis and resistance of essential and nonessential metal ions in bacteria. BxmR from the cyanobacterium Osciliatoria brevis is the first characterized ArsR protein that sen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801313y
更新日期:2008-10-07 00:00:00
abstract::Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990194z
更新日期:1999-08-10 00:00:00
abstract::The simplest carbapenem antibiotic, (5R)-carbapen-2-em-3-carboxylic acid, is biosynthesized from primary metabolites in Pectobacterium carotorova by the action of three enzymes, carboxymethylproline synthase (hereafter named CarB), carbapenam synthetase, and carbapenem synthase. CarB, a member of the crotonase superfa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0483662
更新日期:2004-12-21 00:00:00
abstract::Cyclopropane fatty acid (CFA) synthase of Escherichia coli catalyzes a modification of the acyl chains of phospholipid bilayers. We report (i) identification of the CFA synthase protein, (ii) overproduction (> 600-fold) and purification to essential homogeneity of the enzyme, and (iii) the amino acid sequence of CFA s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a011
更新日期:1992-11-17 00:00:00
abstract::Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010283n
更新日期:2001-08-07 00:00:00
abstract::Type I protein arginine methyltransferases (PRMTs) catalyze asymmetric dimethylation of various proteins, and their dysregulations often correlate with tumorigenesis or developmental deficiency. Recent studies have focused on the in vivo substrate identification and the enzyme mechanism with peptide substrates. Howeve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00995
更新日期:2015-12-29 00:00:00