Hydrolysis of monodisperse phosphatidylcholines by phospholipase A2 occurs on vessel walls and air bubbles.

abstract：:The interaction of the TEM-2 beta-lactamase with 9-deoxyclavulanic acid (3) and with both extensively labeled (2) and specifically labeled (1) clavulanic acid has been studied. The close similarity between 9-doexyclavulanate and clavulanate in kinetics, spectroscopic, and protein chemical terms show that the allyl alc...

journal_title：Biochemistry

authors： Charnas RL,Knowles JR

更新日期：1981-05-26 00:00:00

abstract：:Cuprizone intoxication is a common animal model used to test myelin regenerative therapies for the treatment of diseases such as multiple sclerosis. Mice fed this copper chelator develop reversible, region-specific oligodendrocyte loss and demyelination. While the cellular changes influencing the demyelinating process...

journal_title：Biochemistry

authors： Taraboletti A,Walker T,Avila R,Huang H,Caporoso J,Manandhar E,Leeper TC,Modarelli DA,Medicetty S,Shriver LP

更新日期：2017-03-14 00:00:00

abstract：:The membrane topology of the human apical sodium-dependent bile acid transporter (hASBT) remains unresolved. Whereas N-glycosylation analysis favors a 7 transmembrane (TM) model, membrane insertion scanning supports a 9TM topology. In order to resolve this controversy, we used dual label epitope insertion to systemati...

journal_title：Biochemistry

authors： Banerjee A,Swaan PW

更新日期：2006-01-24 00:00:00

abstract：:Photosensitized oxidation reactions damage tissue by catalyzing the formation of oxyradicals and singlet oxygen. beta-Carotene is hypothesized to exert photoprotective effects by quenching singlet oxygen formed by Type II reactions and by scavenging free radicals formed by Type I reactions. beta-Carotene antioxidant m...

journal_title：Biochemistry

authors： Stratton SP,Liebler DC

更新日期：1997-10-21 00:00:00

abstract：:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...

journal_title：Biochemistry

authors： Thornton K,Gorenstein DG

更新日期：1994-03-29 00:00:00

abstract：:The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...

journal_title：Biochemistry

authors： Greenspoon N,Hershkoviz R,Alon R,Varon D,Shenkman B,Marx G,Federman S,Kapustina G,Lider O

更新日期：1993-02-02 00:00:00

abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...

journal_title：Biochemistry

authors： Rodriguez-Almazan C,Ruiz de Escudero I,Cantón PE,Muñoz-Garay C,Pérez C,Gill SS,Soberón M,Bravo A

更新日期：2011-01-25 00:00:00

abstract：:GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...

journal_title：Biochemistry

authors： Pourjafar-Dehkordi D,Vieweg S,Itzen A,Zacharias M

更新日期：2019-08-20 00:00:00

abstract：:There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...

journal_title：Biochemistry

authors： Chi EY,Frey SL,Lee KY

更新日期：2007-02-20 00:00:00

abstract：:Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...

journal_title：Biochemistry

authors： Barry JK,Matthews KS

更新日期：1999-05-18 00:00:00

abstract：:Phase partition techniques have been used to measure the binding of the antitumor drugs adriamycin (NSC-123127) and daunorubicin (NSC-82151) to various DNAs. These methods provide reliable equilibrium binding data at the low levels of drug binding that may be expected in vivo. Both adriamycin and daunorubicin exhibit ...

journal_title：Biochemistry

authors： Graves DE,Krugh TR

更新日期：1983-08-02 00:00:00

abstract：:Biotin synthase, a member of the "radical SAM" family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin (DTB). The active form of the enzyme contains two iron-sulfur clusters, a [4Fe-4S](2+) cluster liganded by Cys-53, Cys-57, and Cys-60 and the S-ad...

journal_title：Biochemistry

authors： Lotierzo M,Raux E,Tse Sum Bui B,Goasdoue N,Libot F,Florentin D,Warren MJ,Marquet A

更新日期：2006-10-10 00:00:00

abstract：:Triple-helical DNA shows increasing potential for applications in the control of gene expression (including therapeutics) and the development of sequence-specific DNA-cleaving agents. The major limitation in this technology has been the requirement of homopurine sequences for triplex formation. We describe a simple ap...

journal_title：Biochemistry

authors： Jayasena SD,Johnston BH

更新日期：1992-01-21 00:00:00

abstract：:We have investigated the unfolding thermodynamics for incorporating cationic side chains in the Dickerson-Drew dodecamer duplex. Incorporation of two 3-aminopropyl-2'-deoxyuridine residues (one on each self-complementary strand) lowers the stability of the duplex. This reduction is driven by unfavorable heat contribut...

journal_title：Biochemistry

authors： Shikiya R,Li JS,Gold B,Marky LA

更新日期：2005-09-20 00:00:00

abstract：:Light-harvesting complex II (LHC-II) regulates the light energy distribution between photosystem I and II in plants. This process is mediated by phosphorylation of the LHC-II protein, which depends on the oxidation state of photosynthetic electron carriers. In addition to this regulatory mechanism, it has recently bee...

journal_title：Biochemistry

authors： Rogl H,Kühlbrandt W,Barth A

更新日期：2003-09-02 00:00:00

abstract：:Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....

journal_title：Biochemistry

authors： Schimerlik MI,Grimshaw CE,Cleland WW

更新日期：1977-02-22 00:00:00

abstract：:Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...

journal_title：Biochemistry

authors： Schlodder E,Falkenberg K,Gergeleit M,Brettel K

更新日期：1998-06-30 00:00:00

abstract：:Multiple intracellular receptors of the FK506 binding protein (FKBP) family of peptidylprolyl cis/trans-isomerases are potential targets for the immunosuppressive drug FK506. Inhibition of the protein phosphatase calcineurin (CaN), which has been implicated in the FK506-mediated blockade of T cell proliferation, was s...

journal_title：Biochemistry

authors： Weiwad M,Edlich F,Kilka S,Erdmann F,Jarczowski F,Dorn M,Moutty MC,Fischer G

更新日期：2006-12-26 00:00:00

abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...

journal_title：Biochemistry

authors： Pi N,Armstrong JI,Bertozzi CR,Leary JA

更新日期：2002-11-05 00:00:00

abstract：:A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...

journal_title：Biochemistry

authors： Grunau A,Geraki K,Grossmann JG,Gutierrez A

更新日期：2007-07-17 00:00:00

abstract：:The near-ultraviolet circular dichroism (CD) of three homogeneous anti-type III pneumococcal antibodies in the absence and the presence of the specific hexasaccharide ligand was studied. In addition recombinations and hybridizations of H and L chains derived from two of these antibodies were carried out and the CD spe...

journal_title：Biochemistry

authors： Jaton JC,Huser H,Blatt Y,Pecht I

更新日期：1975-12-02 00:00:00

abstract：:The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...

journal_title：Biochemistry

authors： Lipman RS,Jorns MS

更新日期：1996-06-18 00:00:00

abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...

journal_title：Biochemistry

authors： Boussac A,Rutherford AW,Styring S

更新日期：1990-01-09 00:00:00

abstract：:The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and hydroxylamine with sheep liver serine hydroxymethyltransferase (EC 2.1.2.1) was studied by absorption spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of O-amino-D-serine (OADS) with the e...

journal_title：Biochemistry

authors： Baskaran N,Prakash V,Savithri HS,Radhakrishnan AN,Appaji Rao N

更新日期：1989-12-12 00:00:00

abstract：:Prosthetic groups such as heme, chlorophyll, and cobalamin (vitamin B(12)) are characterized by their branched biosynthetic pathway and unique metal insertion steps. The metal ion chelatases can be broadly classed either as single-subunit ATP-independent enzymes, such as the anaerobic cobalt chelatase and the protopor...

journal_title：Biochemistry

authors： Schubert HL,Raux E,Wilson KS,Warren MJ

更新日期：1999-08-17 00:00:00

abstract：:Intramolecular excimer formation with 1,3-di(1-pyrenyl)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2000 or less) without inducing any detectable damage. The temperature was varied between -1 and 40 degrees C. T...

journal_title：Biochemistry

authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

更新日期：1982-11-09 00:00:00

abstract：:Sonication of lysophosphatidylcholine (lysoPC; 20 mumol/mL) and cholesterol (chol) in aqueous medium produces lamellar structures over a wide range of concentrations. From 25 to 47 mol % cholesterol, electron microscopy (EM) after negative staining showed extended stacklike lamellae about 40 A thick. From 50 to 60 mol...

journal_title：Biochemistry

authors： Kumar VV,Anderson WH,Thompson EW,Malewicz B,Baumann WJ

更新日期：1988-01-12 00:00:00

abstract：:Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...

journal_title：Biochemistry

authors： Swaminathan N,Braunstein GD

更新日期：1978-12-26 00:00:00

abstract：:The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...

journal_title：Biochemistry

authors： Oberfeld B,Brunner J,Dimroth P

更新日期：2006-02-14 00:00:00

abstract：:Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...

journal_title：Biochemistry

authors： Swart GW,Parmeggiani A

更新日期：1989-01-10 00:00:00