Abstract：
:The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with reagents carrying a photoactivatable substituent and illuminated. Subunit c and derivatives were then isolated and subjected to mass spectrometric analyses. The most noticeable product, which was found exclusively in irradiated samples, had a mass increase of 719 Da, consistent with a cross-link product between the substituted c subunit and phosphatidylethanolamine. Digestion with phospholipase C converted this product into one with a mass diminished by 126 Da, indicating that the phosphoethanolamine moiety was cleaved off. Hence, the cross-link forms to the diacylglycerol moiety of phosphatidylethanolamine. Control experiments showed that the subunit c-phospholipid adducts were formed in the ATP synthase complex in its natural membrane environment and were not artifacts arising from monomeric c subunits. We conclude therefore that the inner lumen of the c ring is occupied with phospholipids. No evidence was found for an extension of subunit a into this space.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Oberfeld B,Brunner J,Dimroth Pdoi
10.1021/bi052304+subject
Has Abstractpub_date
2006-02-14 00:00:00pages
1841-51issue
6eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901583n
更新日期：2010-01-26 00:00:00
abstract：:Synthetic photocontrolled proteins could be powerful tools for probing cellular chemistry. Several previous attempts to produce such systems by incorporating photoisomerizable chromophores into biomolecules have led to photocontrol but with incomplete reversibility, where the chromophore becomes trapped in one photois...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060142r
更新日期：2006-05-16 00:00:00
abstract：:The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00046a043
更新日期：1995-11-21 00:00:00
abstract：:In the native purple bacterial reaction center (RC), light-driven charge separation utilizes only the A-side cofactors, with the symmetry related B-side inactive. The process is initiated by electron transfer from the excited primary donor (P*) to the A-side bacteriopheophytin (P* --> P+ H(A)-) in approximately 3 ps. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060277x
更新日期：2006-06-13 00:00:00
abstract：:Cdc42 and Rac are highly homologous members of the Rho family of small G proteins that interact with several downstream effector proteins thereby causing cytoskeletal rearrangements, cell proliferation, and differentiation. While some effectors, such as the tyrosine kinase, ACK, and the scaffold protein, WASP, are uni...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0506021
更新日期：2005-09-20 00:00:00
abstract：:The conformations of melittin, an amphipathic polypeptide consisting of 26 amino acid residues, and its hydrophobic (residues 1--19) and hydrophilic (residues 20--26) fragments were examined in various solvent systems, including H2O, 2H2O, 2-chloroethanol, and 1,2-dimyristoylphosphatidylcholine (DMPC) multilayers, by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00539a006
更新日期：1982-05-11 00:00:00
abstract：:The 14-3-3 proteins interact with diverse cellular molecules involved in various signal transduction pathways controlling cell proliferation, transformation, and apoptosis. To aid our investigation of the biological function of 14-3-3 proteins, we have set out to identify high-affinity antagonists. By screening phage ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991353h
更新日期：1999-09-21 00:00:00
abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400652c
更新日期：2013-07-16 00:00:00
abstract：:Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9010607
更新日期：2009-09-08 00:00:00
abstract：:Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. The active enzyme harbors the nickel(I) hydrocorphin coenzyme F-430 as a prosthetic group and catalyzes the reversible reduction of methyl-coenzyme M (CH3-S-CoM) with coenzyme B (HS-CoM) to methane and CoM-S-S-CoB. MCR is als...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.9b00164
更新日期：2019-12-31 00:00:00
abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00316a021
更新日期：1984-10-09 00:00:00
abstract：:Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00648a039
更新日期：1976-02-10 00:00:00
abstract：:The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963095j
更新日期：1997-05-13 00:00:00
abstract：:When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNA(Phe), a single tRNA body was misacylated with 13 different amino acids. The affinities of these misacylated tRNAs for Thermus thermophilus elongation factor Tu (EF-Tu).GTP were determined using a rib...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036290o
更新日期：2004-05-25 00:00:00
abstract：:A recombinant system was developed for generation of steroid-receptor complexes in vitro. The DNA- and steroid-binding domains of the rat mineralocorticoid receptor were expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The identity of the expressed recombinant protein was confirmed by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00084a028
更新日期：1993-08-24 00:00:00
abstract：:Kinesin-5 proteins are essential for formation of a bipolar mitotic spindle in most and, perhaps all, eukaryotic cells. Several Kinesin-5 proteins, notably the human version, HsEg5, are targets of a constantly expanding group of small-molecule inhibitors, which hold promise both as tools for probing mechanochemical tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801291q
更新日期：2009-03-03 00:00:00
abstract：:cis peptide bonds in proteins are often rate-limiting steps in protein folding or conformational change and are frequently stabilized by metal ions. In the collagen-binding domain of Clostridium histolyticum collagenase, the binding of calcium ions triggers the formation of a cis peptide bond. We present free energy s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1007047
更新日期：2010-06-29 00:00:00
abstract：:Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00691a008
更新日期：1975-10-07 00:00:00
abstract：:Tetrachlorohydroquinone dehalogenase catalyzes the replacement of chlorine atoms on tetrachlorohydroquinone and trichlorohydroquinone with hydrogen atoms during the biodegradation of pentachlorophenol by Sphingomonas chlorophenolica. The sequence of the active site region of tetrachlorohydroquinone dehalogenase is ver...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9923813
更新日期：2000-05-09 00:00:00
abstract：:Flavohemoglobins (flavoHbs) are enzymes that operate primarily as nitric oxide dioxygenases and shuttle thereby electrons among NAD(P)H, FAD, heme, and a ligated redox-active substrate such as O(2). They function in the bacterial defense against nitrosative stress and are therefore considered as targets for new antibi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101650q
更新日期：2011-02-22 00:00:00
abstract：:Heme-derived linear tetrapyrroles (phytobilins) in phycobiliproteins and phytochromes perform critical light-harvesting and light-sensing roles in oxygenic photosynthetic organisms. A key enzyme in their biogenesis, phycocyanobilin:ferredoxin oxidoreductase (PcyA), catalyzes the overall four-electron reduction of bili...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100728q
更新日期：2010-07-27 00:00:00
abstract：:The heat-stable, protein inhibitor of the cyclic adenosine monophosphate (cAMP) dependent protein kinase [Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E., & Krebs, E (1971a) J. Biol. Chem. 246, 1977-1985] has been purified to homogeneity from rabbit skeletal muscle by preparative electrophoresis. Em...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00589a011
更新日期：1979-10-30 00:00:00
abstract：:Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a033
更新日期：1995-11-14 00:00:00
abstract：:ATP-binding cassette (ABC) transporters are ubiquitous in all three kingdoms of life and are implicated in many clinically relevant physiological processes. They couple the energy released by ATP hydrolysis to facilitate substrate translocation across cell membranes. The crystal structures of type II ABC importers hav...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00386
更新日期：2016-12-13 00:00:00
abstract：:The protein phosphatase 2C (PP2C) family represents one of the four major protein Ser/Thr phosphatase activities in mammalian cells and contains at least 13 distinct gene products. Although PP2C family members regulate a variety of cellular functions, mechanisms of regulation of their activities are largely unknown. H...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800067p
更新日期：2008-07-08 00:00:00
abstract：:The photochemical intermediate metarhodopsin II (meta II; lambda max = 380 nm) is generally identified with rho*, the conformation of photolyzed rhodopsin which binds and activates the visual G-protein, Gt [Emeis, D., & Hoffman, K.P. (1981) FEBS Lett. 136, 201-207]. Purified bovine rhodopsin was incorporated into vesi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00215a006
更新日期：1991-01-08 00:00:00
abstract：:The importance of the interdomain bridging interactions observed only in the R-state structure of Escherichia coli aspartate transcarbamylase between Glu-50 of the carbamoyl phosphate domain with both Arg-167 and Arg-234 of the aspartate domain has been investigated by using site-specific mutagenesis. Two mutant versi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00458a015
更新日期：1990-02-13 00:00:00
abstract：:Guided by the X-ray structure analysis of a crystalline EcoRV-d(GGGATATCCC) complex (Winkler, in preparation), we have begun to identify functionally important amino acid residues of EcoRV. We show here that Asn70, Asp74, Ser183, Asn185, Thr186, and Asn188 are most likely involved in the binding and/or cleavage of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00240a011
更新日期：1991-07-02 00:00:00
abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00104a022
更新日期：1991-10-08 00:00:00
abstract：:The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050835s
更新日期：2005-09-20 00:00:00
