当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > An unusual pattern of CytR and CRP binding energetics at Escherichia coli cddP suggests a unique blend of class I and class II mediated activation.

An unusual pattern of CytR and CRP binding energetics at Escherichia coli cddP suggests a unique blend of class I and class II mediated activation.

Abstract：

:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both prokaryotic and eukaryotic gene regulation. Analysis of cooperative interactions between CytR and CRP at the deoP2 and udpP promoters has implicated the importance of promoter architecture in controlling repression and induction. These studies have also identified competition between CytR and CRP as an additional contributor to differential regulation. The pattern and energetics of CytR and CRP interactions at the cdd promoter, the most strongly activated of the CytR-regulated promoters, have been delineated using DNase I footprinting. Surprisingly, CRP has greater affinity for the promoter proximal site at cddP, CRP1, than for the distal site, CRP2, in contrast to promoters studied previously. This difference is a major contributor to unusually high CRP-mediated activation of cddP. Additionally, while cytidine binding to CytR nearly eliminates the pairwise interactions between CytR and CRP bound at CRP1, it has little effect on pairwise cooperativity between CytR and CRP bound at CRP2 or as a consequence on the overall cooperativity of the three-protein complex in which CRP is bound to both sites. The effect of cytidine binding on cooperativity differs between the three promoters studied thus far. We propose that the different patterns of interaction reflect the spacing between CytR half-sites and the location of the CytR operator in relation to the two CRP sites.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Holt AK,Senear DF

doi

10.1021/bi901583n

subject

Has Abstract

pub_date

2010-01-26 00:00:00

pages

432-42

issue

3

eissn

0006-2960

issn

1520-4995

journal_volume

49

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • NMR structures of the C-terminal segment of surfactant protein B in detergent micelles and hexafluoro-2-propanol.

    abstract：:Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0481895

    authors： Booth V,Waring AJ,Walther FJ,Keough KM

    更新日期：2004-12-07 00:00:00

  • Crystal structure of a prolonged-acting insulin with albumin-binding properties.

    abstract：:The fatty acid acylated insulin, Lys(B29)-tetradecanoyl, des-(B30) human insulin, has been crystallized and the structure determined by X-ray crystallography. The fatty acid substituent on residue B29 Lys binds reversibly to circulating albumin protein in vivo, and by this mechanism the hormone's action is prolonged. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9625105

    authors： Whittingham JL,Havelund S,Jonassen I

    更新日期：1997-03-11 00:00:00

  • Cu(II) coordination in arthropod and mollusk green half-methemocyanins analyzed by electron spin-echo envelope modulation spectroscopy.

    abstract：:Hemocyanin (Hc) is a dinuclear copper protein that binds oxygen reversibly. The structure of the Cu(II) site in a derivative of hemocyanin known as green half-met (GHM) has been analyzed using the pulsed EPR technique of electron spin-echo envelope modulation (ESEEM) spectroscopy. The derivative, prepared by treating ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00005a007

    authors： Magliozzo RS,Bubacco L,McCracken J,Jiang F,Beltramini M,Salvato B,Peisach J

    更新日期：1995-02-07 00:00:00

  • Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7.

    abstract：:The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701949p

    authors： Collino S,Kim IW,Evans JS

    更新日期：2008-03-25 00:00:00

  • Cloning, expression, and characterization of human cytosolic aminopeptidase P: a single manganese(II)-dependent enzyme.

    abstract：:The mammalian bradykinin-degrading enzyme aminopeptidase P (AP-P; E. C. 3.4.11.9) is a metal-dependent enzyme and is a member of the peptidase clan MG. AP-P exists as membrane-bound and cytosolic forms, which represent distinct gene products. A partially truncated clone encoding the cytosolic form was obtained from a ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001585c

    authors： Cottrell GS,Hooper NM,Turner AJ

    更新日期：2000-12-12 00:00:00

  • A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays.

    abstract：:We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that se...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500500h

    authors： Yang Y,Zhang Q,Gao M,Yang X,Huang Z,An J

    更新日期：2014-08-05 00:00:00

  • Effects of metal ligation and oxygen on the reversibility of the thermal denaturation of Pseudomonas aeruginosa azurin.

    abstract：:Thermodynamic equilibrium transition models in DSC are only applicable to reversible processes, but reversibility of the thermal transitions of proteins is comparatively rare because of intermolecular aggregation of denatured proteins and the degradation that occurs at high temperatures. The cupredoxin azurin from Pse...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0157621

    authors： Sandberg A,Leckner J,Shi Y,Schwarz FP,Karlsson BG

    更新日期：2002-01-22 00:00:00

  • Role of lysines in human angiogenin: chemical modification and site-directed mutagenesis.

    abstract：:The role of lysines in the ribonucleolytic and angiogenic activities of human angiogenin has been examined by chemical modification and site-directed mutagenesis. It was demonstrated previously [Shapiro, R., Weremowicz, S., Riordan, J.F., & Vallee, B.L. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 8783-8787] that extensiv...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00430a045

    authors： Shapiro R,Fox EA,Riordan JF

    更新日期：1989-02-21 00:00:00

  • Bis(pyrrolecarboxamide) linked to intercalating chromophore oxazolopyridocarbazole (OPC): selective binding to DNA and polynucleotides.

    abstract：:We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00220a029

    authors： Subra F,Carteau S,Pager J,Paoletti J,Paoletti C,Auclair C,Mrani D,Gosselin G,Imbach JL

    更新日期：1991-02-12 00:00:00

  • A solution NMR investigation into the early events of amelogenin nanosphere self-assembly initiated with sodium chloride or calcium chloride.

    abstract：:Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8018288

    authors： Buchko GW,Tarasevich BJ,Bekhazi J,Snead ML,Shaw WJ

    更新日期：2008-12-16 00:00:00

  • A remote substrate docking mechanism for the tec family tyrosine kinases.

    abstract：:During T cell signaling, Itk selectively phosphorylates a tyrosine within its own SH3 domain and a tyrosine within PLCgamma1. We find that the remote SH2 domain in each of these substrates is required to achieve efficient tyrosine phosphorylation by Itk and extend this observation to two other Tec family kinases, Btk ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700127c

    authors： Joseph RE,Min L,Xu R,Musselman ED,Andreotti AH

    更新日期：2007-05-08 00:00:00

  • A monoclonal antibody against brain calmodulin-dependent protein kinase type II detects putative conformational changes induced by Ca2+-calmodulin.

    abstract：:A mouse monoclonal IgG1 antibody has been generated against the soluble form of the calmodulin-dependent protein kinase type II. This antibody recognizes both the soluble and cytoskeletal forms of the enzyme, requiring Ca2+ (EC50 = 20 microM) for the interaction. Other divalent cations such as Zn2+, Mn2+, Cd2+, Co2+, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00417a060

    authors： LeVine H 3rd,Su JL,Sahyoun NE

    更新日期：1988-08-23 00:00:00

  • Effect of an Imposed Contact on Secondary Structure in the Denatured State of Yeast Iso-1-cytochrome c.

    abstract：:There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01002

    authors： Danielson TA,Stine JM,Dar TA,Briknarova K,Bowler BE

    更新日期：2017-12-26 00:00:00

  • Purification and properties of squirrel monkey (Saimiri sciureus) corticosteroid binding globulin.

    abstract：:Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00407a046

    authors： Kuhn RW,VestWeber C,Siiteri PK

    更新日期：1988-04-05 00:00:00

  • Plasticity of amyloid fibrils.

    abstract：:In experiments designed to characterize the basis of amyloid fibril stability through mutational analysis of the Abeta (1-40) molecule, fibrils exhibit consistent, significant structural malleability. In these results, and in other properties, amyloid fibrils appear to more resemble plastic materials generated from sy...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi0620959

    authors： Wetzel R,Shivaprasad S,Williams AD

    更新日期：2007-01-09 00:00:00

  • 8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions.

    abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9907835

    authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

    更新日期：1999-10-12 00:00:00

  • Structure, topology, and tilt of cell-signaling peptides containing nuclear localization sequences in membrane bilayers determined by solid-state NMR and molecular dynamics simulation studies.

    abstract：:Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061895g

    authors： Ramamoorthy A,Kandasamy SK,Lee DK,Kidambi S,Larson RG

    更新日期：2007-01-30 00:00:00

  • Escherichia coli cAMP receptor protein-DNA complexes. 1. Energetic contributions of half-sites and flanking sequences in DNA recognition.

    abstract：:In Escherichia coli, the cyclic AMP receptor protein (CRP) serves as a sensor of the intracellular level of cyclic AMP. At increasing concentrations of cyclic AMP, CRP becomes activated upon binding a cyclic AMP molecule. The activated CRP is capable of regulating the expression of more than 20 genes by binding to spe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972450i

    authors： Pyles EA,Chin AJ,Lee JC

    更新日期：1998-04-14 00:00:00

  • Dyrk1A binds to multiple endocytic proteins required for formation of clathrin-coated vesicles.

    abstract：:In spite of a nuclear targeting sequence, a substantial amount of dual-specificity tyrosine phosphorylation-regulated kinase (Dyrk1A) is located within the cytoplasm of neurons. Analysis of fractionated rat brains revealed that the majority of Dyrk1A was in the postnuclear precipitate. The kinase in this fraction was ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9010557

    authors： Murakami N,Bolton D,Hwang YW

    更新日期：2009-10-06 00:00:00

  • Spectroscopic and functional characterization of T state hemoglobin conformations encapsulated in silica gels.

    abstract：:Oxygen binding curves of sol-gel-encapsulated deoxy human adult hemoglobin (HbA) have previously revealed two distinct noncooperative populations with oxygen binding affinities approximately 1000 and 100 times lower than that of the high-affinity R state. The two populations which have been termed the low-affinity (LA...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048531d

    authors： Samuni U,Dantsker D,Juszczak LJ,Bettati S,Ronda L,Mozzarelli A,Friedman JM

    更新日期：2004-11-02 00:00:00

  • Olfactory transduction: cross-talk between second-messenger systems.

    abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00469a004

    authors： Anholt RR,Rivers AM

    更新日期：1990-05-01 00:00:00

  • A single amino acid substitution in the human and a bacterial hypoxanthine phosphoribosyltransferase modulates specificity for the binding of guanine.

    abstract：:Early studies involving purine salvage in Salmonella typhimurium resulted in the isolation and identification of a mutant strain possessing a genetically modified hypoxanthine phosphoribosyl-transferase (HPRT) with enhanced substrate specificity for guanine [Benson, C. E., and Gots, J. S. (1975) J. Bacteriol. 121, 77-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9720179

    authors： Lee CC,Craig SP 3rd,Eakin AE

    更新日期：1998-03-10 00:00:00

  • Mechanism of translocation and kinetics of DNA unwinding by the helicase RecG.

    abstract：:RecG is a DNA helicase involved in the repair of damage at a replication fork and catalyzes the reversal of the fork to a point beyond the damage in the template strand. It unwinds duplex DNA in reactions that are coupled to ATP hydrolysis. The kinetic mechanism of duplex DNA unwinding by RecG was analyzed using a qua...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0512851

    authors： Martinez-Senac MM,Webb MR

    更新日期：2005-12-27 00:00:00

  • Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association.

    abstract：:Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701651k

    authors： Fuson KL,Montes M,Robert JJ,Sutton RB

    更新日期：2007-11-13 00:00:00

  • Fluorescence energy transfer measurements of spatial relationships between sulfhydryl groups of thiolase I from porcine heart.

    abstract：:Mitochondrial thiolase I from pig heart has been found to have at least two and possibly three reactive sulfhydryl residues at or near the active site [Izbicka-Dimitrijević, E., & Gilbert, H. F. (1982) Biochemistry 21, 6112-6118; Izbicka-Dimitrijević, E., & Gilbert, H. F. (1984) Biochemistry 23, 4318-4324]. In the nat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00321a015

    authors： Izbicka E,Gilbert HF

    更新日期：1984-12-18 00:00:00

  • Role of two acetylcholine receptor subunit domains in homomer formation and intersubunit recognition, as revealed by alpha 3 and alpha 7 subunit chimeras.

    abstract：:Differential expression of subunit genes from the nicotinic acetylcholine receptor (AChR) superfamily yields distinct receptor subtypes. As each AChR subtype has a specific subunit composition and many subunit combinations appear not to be expressed, each subunit must contain some information leading to proper assembl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00254a031

    authors： García-Guzmán M,Sala F,Sala S,Campos-Caro A,Criado M

    更新日期：1994-12-20 00:00:00

  • NMR studies of the association of cytochrome b5 with cytochrome c.

    abstract：:In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001129o

    authors： Hom K,Ma QF,Wolfe G,Zhang H,Storch EM,Daggett V,Basus VJ,Waskell L

    更新日期：2000-11-21 00:00:00

  • Cholesterol efflux from macrophage foam cells is enhanced by active phospholipid transfer protein through generation of two types of acceptor particles.

    abstract：:Phospholipid transfer protein (PLTP) is expressed by macrophage-derived foam cells in human atherosclerotic lesions, suggesting a regulatory role for PLTP in cellular cholesterol homeostasis. However, the exact role of PLTP in the reverse cholesterol transport pathway is not known. PLTP is present in plasma as two for...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700833h

    authors： Vikstedt R,Metso J,Hakala J,Olkkonen VM,Ehnholm C,Jauhiainen M

    更新日期：2007-10-23 00:00:00

  • General peroxidase activity of G-quadruplex-hemin complexes and its application in ligand screening.

    abstract：:DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9006786

    authors： Cheng X,Liu X,Bing T,Cao Z,Shangguan D

    更新日期：2009-08-25 00:00:00

  • Structural characterization of the ATP-hydrolyzing portion of the coated vesicle proton pump.

    abstract：:The ATP-hydrolyzing portion of the proton pump from clathrin-coated vesicles (isolated from calf brain) was solubilized with three nondenaturing detergents (cholate, octyl glucoside, and Triton X-100). The hydrodynamic properties of the solubilized (Mg2+)-ATPase were then determined by sedimentation analysis in H2O an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00363a015

    authors： Forgac M,Berne M

    更新日期：1986-07-29 00:00:00