Abstract:
:We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yang Y,Zhang Q,Gao M,Yang X,Huang Z,An Jdoi
10.1021/bi500500hsubject
Has Abstractpub_date
2014-08-05 00:00:00pages
4881-3issue
30eissn
0006-2960issn
1520-4995journal_volume
53pub_type
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