当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Dissociation of apolipoprotein E oligomers to monomer is required for high-affinity binding to phospholipid vesicles.

Dissociation of apolipoprotein E oligomers to monomer is required for high-affinity binding to phospholipid vesicles.

Abstract：

:The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molecular weight form of apoE that binds to lipid has not been clearly defined. We have examined the role of self-association of apoE with respect to interactions with phospholipids. Measurements of the time dependence of turbidity clearance of small unilamellar vesicles of dimyristoyl-sn-glycero-3-phosphocholine (DMPC) upon addition of apoE show that higher molecular weight oligomers bind poorly if at all. The kinetic data can be described by a reaction model in which tetramers and dimers of apoE must first dissociate to monomers which then bind to the liposome surface in a fast and reversible manner. A slow but not readily reversible conformational conversion of the monomer then occurs. Prior knowledge of the rate constants for the association-dissociation process allows us to determine the rate constant of the conformational conversion. This rate constant is isoform dependent and appears to correlate with the stability of the apoE isoforms with the rate of dissociation of the apoE oligomers to monomers being the rate-limiting process for lipidation. Differences in the lipidation kinetics between the apoE isoforms arise from their differences in the self-association behavior leading to the conclusion that self-association behavior may influence biological functions of apoE in an isoform-dependent manner.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Garai K,Baban B,Frieden C

doi

10.1021/bi1020106

subject

Has Abstract

pub_date

2011-04-05 00:00:00

pages

2550-8

issue

13

eissn

0006-2960

issn

1520-4995

journal_volume

50

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structure of glucagon-like peptide (7-36) amide in a dodecylphosphocholine micelle as determined by 2D NMR.

    abstract：:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00178a009

    authors： Thornton K,Gorenstein DG

    更新日期：1994-03-29 00:00:00

  • Role of arginine-292 in the substrate specificity of aspartate aminotransferase as examined by site-directed mutagenesis.

    abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00412a052

    authors： Cronin CN,Kirsch JF

    更新日期：1988-06-14 00:00:00

  • Phenotypically selected mutations in myosin's actin binding domain demonstrate intermolecular contacts important for motor function.

    abstract：:Here, we biochemically characterize Dictyostelium myosin II mutants that were previously phenotypically selected following random mutagenesis and shown to lie in the actin binding domain [Patterson, B., & Spudich, J. A. (1996) Genetics 143, 801-810]. We show that the conditional loss of myosin-dependent activity in vi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi963141f

    authors： Giese KC,Spudich JA

    更新日期：1997-07-15 00:00:00

  • Ribosome subunit to polysome ratios affect the synthesis of rRNA in Drosophila cells.

    abstract：:Many investigations have revealed that ribosome numbers increase in parallel with the growth rate of cells. Here we show that the absolute level of protein synthesis may not be the only factor influencing rRNA synthesis in a nondividing eukaryotic cell. Under conditions of complete (greater than 99%) inhibition of pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00502a003

    authors： Yamamoto KK,Pellegrini M

    更新日期：1990-12-18 00:00:00

  • Binding of O(2) and its reduction are both retarded by replacement of valine 279 by isoleucine in cytochrome c oxidase from Paracoccus denitrificans.

    abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000123w

    authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

    更新日期：2000-05-30 00:00:00

  • Role of structural and energetic factors in regulating repair of a bulky DNA lesion with different opposite partner bases.

    abstract：:Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4009177

    authors： Mu H,Kropachev K,Chen Y,Zhang H,Cai Y,Geacintov NE,Broyde S

    更新日期：2013-08-20 00:00:00

  • Modification of the conformational equilibria in the sodium and potassium dependent adenosinetriphosphatase with glutaraldehyde.

    abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00288a016

    authors： Chipman DM,Lev A

    更新日期：1983-09-13 00:00:00

  • Role of conserved active site residues in catalysis by phospholipase B1 from Cryptococcus neoformans.

    abstract：:Phospholipase B1 (PLB1), secreted by the pathogenic yeast Cryptococcus neoformans, has an established role in virulence. Although the mechanism of its phospholipase B, lysophospholipase, and lysophospholipase transacylase activities is unknown, it possesses lipase, subtilisin protease aspartate, and phospholipase moti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7009508

    authors： Jones PM,Turner KM,Djordjevic JT,Sorrell TC,Wright LC,George AM

    更新日期：2007-09-04 00:00:00

  • Oligonucleotide-directed nucleic acid scission by micrococcal nuclease.

    abstract：:"Sequence-dictated" scission of DNA can be achieved by tethering a fusion protein composed of glutathione S-transferase and attenuated micrococcal nuclease (MN) to a targeting oligonucleotide using Cibacron blue (CB) F3G-A. Deoxyoligonucleotides derivatized with this dye bind to the fusion protein in gel mobility shif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00201a006

    authors： Landgraf R,Chen CH,Sigman DS

    更新日期：1994-09-06 00:00:00

  • Influence of cytosolic AGS3 on receptor--G protein coupling.

    abstract：:Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. It competes with Gbetagamma subunits to bind GDP-bound Gi/o(alpha) subunits via four repeated G protein regulatory (GPR) domains in the carboxyl-terminal half of the mol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034561p

    authors： Ma H,Peterson YK,Bernard ML,Lanier SM,Graber SG

    更新日期：2003-07-08 00:00:00

  • Progressive Rod-Cone Degeneration (PRCD) Protein Requires N-Terminal S-Acylation and Rhodopsin Binding for Photoreceptor Outer Segment Localization and Maintaining Intracellular Stability.

    abstract：:The light-sensing outer segments of photoreceptor cells harbor hundreds of flattened membranous discs containing the visual pigment, rhodopsin, and all the proteins necessary for visual signal transduction. PRCD (progressive rod-cone degeneration) protein is one of a few proteins residing specifically in photoreceptor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00489

    authors： Spencer WJ,Pearring JN,Salinas RY,Loiselle DR,Skiba NP,Arshavsky VY

    更新日期：2016-09-13 00:00:00

  • Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin.

    abstract：:Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300688k

    authors： Petrillo T,O'Donohoe CA,Howe N,Malthouse JP

    更新日期：2012-08-07 00:00:00

  • Studies on the microsomal mixed function oxidase system: redox properties of detergent-solubilized NADPH-cytochrome P-450 reductase.

    abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00604a032

    authors： Iyanagi T,Anan FK,Imai Y,Mason HS

    更新日期：1978-05-30 00:00:00

  • Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains.

    abstract：:We previously reported that the microtubule (MT)-binding domain of microtubule-associated protein 4 (MAP4) contains three 18-amino acid imperfect repeats that are homologous to the repeats found in the MT-binding domains of the neuronal MAPs, MAP2 and tau [Chapin, S. J., & Bulinski, J. C. (1991) J. Cell Sci. 98, 27-36...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00007a025

    authors： Chapin SJ,Lue CM,Yu MT,Bulinski JC

    更新日期：1995-02-21 00:00:00

  • Characterization of the interaction between androgen receptor and a new transcriptional inhibitor, SHP.

    abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011384o

    authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

    更新日期：2001-12-18 00:00:00

  • Mycobacterium tuberculosis Rv0899 adopts a mixed alpha/beta-structure and does not form a transmembrane beta-barrel.

    abstract：:The membrane protein Rv0899 (OmpATb) from Mycobacterium tuberculosis, has been proposed to act as an outer membrane porin and to contribute to the bacterium's adaptation to the acidic environment of the phagosome during infection. The gene is restricted to pathogenic mycobacteria and, thus, is an attractive candidate ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100158s

    authors： Teriete P,Yao Y,Kolodzik A,Yu J,Song H,Niederweis M,Marassi FM

    更新日期：2010-04-06 00:00:00

  • The gene for human protein Z is localized to chromosome 13 at band q34 and is coded by eight regular exons and one alternative exon.

    abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972002a

    authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

    更新日期：1998-05-12 00:00:00

  • Tubulin polymerization promoting proteins (TPPPs): members of a new family with distinct structures and functions.

    abstract：:TPPP/p25 is a brain-specific protein, which induces tubulin polymerization and microtubule (MT) bundling and is enriched in Lewy bodies characteristic of Parkinson's disease [Tirián et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13976-13981]. We identified two human gene sequences, CG1-38 and p25beta, which encoded ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061305e

    authors： Vincze O,Tökési N,Oláh J,Hlavanda E,Zotter A,Horváth I,Lehotzky A,Tirián L,Medzihradszky KF,Kovács J,Orosz F,Ovádi J

    更新日期：2006-11-21 00:00:00

  • Solution structures of spinach acyl carrier protein with decanoate and stearate.

    abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052062d

    authors： Zornetzer GA,Fox BG,Markley JL

    更新日期：2006-04-25 00:00:00

  • Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: the importance of the cyclic cystine knot.

    abstract：:The cyclotides constitute a recently discovered family of plant-derived peptides that have the unusual features of a head-to-tail cyclized backbone and a cystine knot core. These features are thought to contribute to their exceptional stability, as qualitatively observed during experiments aimed at sequencing and char...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049711q

    authors： Colgrave ML,Craik DJ

    更新日期：2004-05-25 00:00:00

  • Identification of polyphosphate recognition sites communicating with actin sites on the skeletal myosin subfragment 1 heavy chain.

    abstract：:Using the thrombin-cut [68-30 kilodalton (kDa)] myosin subfragment 1 (S-1) whose heavy chain has been selectively split within the central 50-kDa region, at Lys-560, with concomitant specific alterations of the ATPase and actin binding properties [Chaussepied, P., Mornet, D., Audemard, E., Derancourt, J., & Kassab, R....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a013

    authors： Chaussepied P,Mornet D,Kassab R

    更新日期：1986-10-21 00:00:00

  • Dissection of porphyrin-induced conformational dynamics in the heme biosynthesis enzyme ferrochelatase.

    abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300704c

    authors： Asuru AP,An M,Busenlehner LS

    更新日期：2012-09-11 00:00:00

  • Identification of the amatoxin-binding subunit of RNA polymerase B by affinity labeling experiments. Subunit B3-the true amatoxin receptor protein of multiple RNA polymerase B.

    abstract：:Crude calf thymus DNA-dependent RNA polymerase, RNA polymerase B (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6), was incubated with the tritium labeled, potent inhibitor [3H]amanin, in order to form the enzymatically inactive [3H]amanin-polymerase complex ([3H]A-P complex). Subsequent purificati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00661a013

    authors： Brodner OG,Wieland T

    更新日期：1976-08-10 00:00:00

  • 24-homologated 1,25-dihydroxyvitamin D3 compounds: separation of calcium and cell differentiation activities.

    abstract：:A series of 24-homologated 1,25-dihydroxyvitamin D3 compounds have been chemically synthesized and studied with regard to their activity in inducing differentiation of human promyelocyte HL-60 cells to monocytes and in calcium mobilizing activity in vitamin D deficient rats. Homologation of 1,25-dihydroxyvitamin D3 or...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00453a026

    authors： Perlman K,Kutner A,Prahl J,Smith C,Inaba M,Schnoes HK,DeLuca HF

    更新日期：1990-01-09 00:00:00

  • Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling.

    abstract：:Thermococcus litoralis 4-alpha-glucanotransferase (TLGT) belongs to family 57 of glycoside hydrolases and catalyzes the disproportionation and cycloamylose synthesis reactions. Family 57 glycoside hydrolases have not been well investigated, and even the catalytic mechanism involving the active site residues has not be...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011017c

    authors： Imamura H,Fushinobu S,Jeon BS,Wakagi T,Matsuzawa H

    更新日期：2001-10-16 00:00:00

  • Interactions of a Bacterial Cu(I)-ATPase with a Complex Lipid Environment.

    abstract：:Phospholipids and sterols play multiple roles in cells. In addition to establishing barriers between compartments, they also provide the matrix for assembly and function of a large variety of catalytic processes. Lipid composition is a highly regulated feature of biological membranes, yet its implications for membrane...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00326

    authors： Autzen HE,Koldsø H,Stansfeld PJ,Gourdon P,Sansom MSP,Nissen P

    更新日期：2018-07-17 00:00:00

  • Internal dynamics of the tryptophan repressor (TrpR) and two functionally distinct TrpR variants, L75F-TrpR and A77V-TrpR, in their l-Trp-bound forms.

    abstract：:Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200389k

    authors： Tripet BP,Goel A,Copie V

    更新日期：2011-06-14 00:00:00

  • Ferrate oxidation of murine leukemia virus reverse transcriptase: identification of the template-primer binding domain.

    abstract：:Treatment of murine leukemia virus reverse transcriptase (MuLV RT) with potassium ferrate, an oxidizing agent known to oxidize amino acids involved in phosphate binding domains of proteins, results in the irreversible inactivation of both the DNA polymerase and the RNase H activities. Significant protection from ferra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00247a015

    authors： Reddy G,Nanduri VB,Basu A,Modak MJ

    更新日期：1991-08-20 00:00:00

  • Interactions of myelin basic protein with mixed dodecylphosphocholine/palmitoyllysophosphatidic acid micelles.

    abstract：:The interactions of myelin basic protein and peptides derived from it with detergent micelles of lysophosphatidylglycerol, lysophosphatidylserine, palmitoyllysophosphatidic acid, and sodium lauryl sulfate, and with mixed micelles of the neutral detergent dodecylphosphocholine and the negatively charged detergent palmi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00461a014

    authors： Mendz GL,Brown LR,Martenson RE

    更新日期：1990-03-06 00:00:00

  • Urea-induced unfolding of Na,K-ATPase as evaluated by electron paramagnetic resonance spectroscopy.

    abstract：:Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901124j

    authors： Babavali M,Esmann M,Fedosova NU,Marsh D

    更新日期：2009-09-29 00:00:00