Abstract:
:Lecithin retinol acyltransferase (LRAT) catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction in the vertebrate visual cycle. Since all-trans-retinyl esters are the substrates for the isomerization reaction that generates 11-cis-retinoids, this esterification reaction is essential in the operation of the visual cycle. In addition, LRAT is the founder member of a series of proteins, which are of novel sequence and have unknown functions. Native LRAT is an integral membrane protein and has never been purified. To obtain a pure LRAT, the N- and C-transmembrane termini were deleted and replaced with a poly His tag for the purpose of purification. This truncated form of LRAT, referred to as tLRAT, has been expressed in bacteria and fully purified. tLRAT is catalytically active and processes all-trans-retinol at least 10-fold more efficiently than 11-cis-retinol, the precursor to the visual chromophore. While tLRAT can be robustly expressed in bacteria, it requires detergent for extraction, as the enzyme still contains hydrophobic domains, which may interact. Indeed, tLRAT can oligomerize and forms dimers. Native LRAT also forms functional homodimers. These studies pave the way for the preparation of large-scale amounts of pure tLRAT for further mechanistic and structural studies.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bok D,Ruiz A,Yaron O,Jahng WJ,Ray A,Xue L,Rando RRdoi
10.1021/bi0342416subject
Has Abstractpub_date
2003-05-27 00:00:00pages
6090-8issue
20eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00643a033
更新日期:1977-11-29 00:00:00
abstract::Glycosaminoglycans (GAGs) have been reported to play a significant role in amyloid formation of a wide range of proteins/peptides either associated with diseases or native biological functions. The exact mechanism by which GAGs influence amyloid formation is not clearly understood. Here, we studied two closely related...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401398k
更新日期:2013-12-10 00:00:00
abstract::The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0500832
更新日期:2005-06-21 00:00:00
abstract::The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9910880
更新日期:1999-09-14 00:00:00
abstract::By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00393a015
更新日期:1987-09-22 00:00:00
abstract::kappa-Bungarotoxin (kappa-bgt) is a 66-residue peptide originally purified from snake venom that acts as an antagonist at certain acetylcholine receptors. It is one of four homologous kappa-neurotoxins that are distinguished from the structurally related alpha-neurotoxins by their ability to block the alpha 3-subunit-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00248a004
更新日期:1994-11-08 00:00:00
abstract::Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01242
更新日期:2018-03-13 00:00:00
abstract::The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00657a032
更新日期:1976-06-15 00:00:00
abstract::Arginine-70 of human dihydrofolate reductase (hDHFR) is a highly conserved residue which X-ray crystallographic data have shown to interact with the alpha-carboxylate of the terminal L-glutamate moiety of either folic acid or methotrexate (MTX). The rationale for this study was to introduce a conservative amino acid r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00247a005
更新日期:1991-08-20 00:00:00
abstract::Substitution by site-directed mutagenesis of any one of the histidine residues H721, H837, and H852 by glutamine, or of H752 by serine, inhibits Cl- flux mediated by band 3 expressed in Xenopus oocytes. Mutation of Lys 558 (K558N), the site of covalent binding of H2DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonate) i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00029a006
更新日期:1995-07-25 00:00:00
abstract::The effects of urea on apomyoglobin solubility have been investigated. Apomyoglobin precipitation was found to be a thermodynamically reversible process independent of the pathway of aggregation. A liquid-solid phase diagram was constructed for the precipitation of apomyoglobin as a function of urea and protein concen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00066a006
更新日期:1993-04-20 00:00:00
abstract::The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00228a023
更新日期:1991-04-09 00:00:00
abstract::The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0509402
更新日期:2005-09-27 00:00:00
abstract::A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952993x
更新日期:1996-08-13 00:00:00
abstract::Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301369b
更新日期:2013-02-05 00:00:00
abstract::Bovine brain adenylate cyclase was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium cholate, sodium deoxycholate, or these detergents plus (NH4)2SO4. The specific activity of the extract obtained with 13 mM CHAPS alone was several times those of the other detergent extracts wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00258a019
更新日期:1982-07-20 00:00:00
abstract::The cytoplasmic disassembly of Ran.GTP.importin and Ran.GTP.exportin. cargo complexes is an essential step in the corresponding nuclear import and export cycles. It has previously been shown that such disassembly can be mediated by RanBP1 in the presence of RanGAP. The nuclear pore complex protein RanBP2 (Nup358) cont...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001010f
更新日期:2000-09-26 00:00:00
abstract::We have examined the binding of 125I-labeled human and mouse epidermal growth factors (EGF) to human alpha 2-macroglobulin (alpha 2M). In the presence of human neutrophil elastase, both mouse and human EGF bound to alpha 2M, whereas little binding was found to native alpha 2M. Binding was found to be predominantly cov...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00082a012
更新日期:1993-08-10 00:00:00
abstract::PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9011594
更新日期:2009-12-08 00:00:00
abstract::Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300113x
更新日期:2012-07-31 00:00:00
abstract::Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990286o
更新日期:1999-07-06 00:00:00
abstract::Patas monkeys were studied for 2 years on three dietary regimes: (1) commercial chow (control diet); (2) semipurified diet plus lard (fat-fed); and (3) semipurified diet plus lard and cholesterol (cholesterol-fed). The control and fat-fed animals had similar lipoproteins which were equivalent to the human very low den...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00659a007
更新日期:1976-07-13 00:00:00
abstract::Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025654u
更新日期:2002-06-25 00:00:00
abstract::To investigate the possible role of viral glycoprotein mobility in membrane fusion, fluorescence photobleaching recovery was employed to study the effects of exposure to mildly acidic pH (required to convert many viral fusion proteins to the fusion-active form) on the lateral mobility of influenza hemagglutinin (HA) p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00052a014
更新日期:1993-01-12 00:00:00
abstract::The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0004596
更新日期:2000-08-08 00:00:00
abstract::Members of the uracil-DNA glycosylase (UDG) enzyme family recognize and bind uracil, sequestering it within the binding site pocket and catalyzing the cleavage of the base from the deoxyribose, leaving an abasic site. The recognition and binding are passive and rely on innate dynamic motions of DNA wherein base pairs ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00676
更新日期:2018-10-02 00:00:00
abstract::Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00350a022
更新日期:1986-01-28 00:00:00
abstract::The role of bacterial DnaJ protein as a cochaperone of DnaK is strongly appreciated. Although DnaJ unaccompanied by DnaK can bind unfolded as well as native substrate proteins, its role as an individual chaperone remains elusive. In this study, we demonstrate that DnaJ binds a model non-native substrate with a low nan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301543g
更新日期:2013-02-12 00:00:00
abstract::The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG or in the standard buffer medium fo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961724j
更新日期:1997-01-07 00:00:00
abstract::Src homology 2 (SH2) domains are phosphotyrosine- (pY-) binding modules found in a variety of signal-transducing proteins and constitute an important class of drug targets for the treatment of signaling related diseases/conditions. To date, a large number of peptidic as well as nonpeptidic SH2 domain inhibitors have b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034076u
更新日期:2003-05-06 00:00:00