Local and global dynamics of the G protein-coupled receptor CXCR1.


:The local and global dynamics of the chemokine receptor CXCR1 are characterized using a combination of solution NMR and solid-state NMR experiments. In isotropic bicelles (q = 0.1), only 13% of the expected number of backbone amide resonances is observed in (1)H/(15)N HSQC solution NMR spectra of uniformly (15)N-labeled samples; extensive deuteration and the use of TROSY made little difference in the 800 MHz spectra. The limited number of observed amide signals is ascribed to mobile backbone sites and assigned to specific residues in the protein; 19 of the signals are from residues at the N-terminus and 25 from residues at the C-terminus. The solution NMR spectra display no evidence of local backbone motions from residues in the transmembrane helices or interhelical loops of CXCR1. This finding is reinforced by comparisons of solid-state NMR spectra of both magnetically aligned and unoriented bilayers containing either full-length or doubly N- and C-terminal truncated CXCR1 constructs. CXCR1 undergoes rapid rotational diffusion about the normal of liquid crystalline phospholipid bilayers; reductions in the frequency span and a change to axial symmetry are observed for both carbonyl carbon and amide nitrogen chemical shift powder patterns of unoriented samples containing (13)C- and (15)N-labeled CXCR1. In contrast, when the phospholipids are in the gel phase, CXCR1 does not undergo rapid global reorientation on the 10(4) Hz time scale defined by the carbonyl carbon and amide nitrogen chemical shift powder patterns.






Park SH,Casagrande F,Das BB,Albrecht L,Chu M,Opella SJ




Has Abstract


2011-03-29 00:00:00












  • 1H NMR study of the base-pairing reactions of d(GGAATTCC): salt and polyamine effects on the imino proton exchange.

    abstract::Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...


    pub_type: 杂志文章


    authors: Braunlin WH,Bloomfield VA

    更新日期:1988-02-23 00:00:00

  • Rates of oxygen and hydrogen exchange as indicators of TPQ cofactor orientation in amine oxidases.

    abstract::This study presents the first detailed examination by resonance Raman (RR) spectroscopy of the rates of solvent exchange for the C5 and C3 positions of the TPQ cofactor in several wild-type copper-containing amine oxidases and mutants of the amine oxidase from Hansenula polymorpha (HPAO). On the basis of crystal struc...


    pub_type: 杂志文章


    authors: Green EL,Nakamura N,Dooley DM,Klinman JP,Sanders-Loehr J

    更新日期:2002-01-15 00:00:00

  • Light inhibition of bovine retinal rod guanylyl cyclase mediated by beta gamma-transducin.

    abstract::Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...


    pub_type: 杂志文章


    authors: Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

    更新日期:1999-03-02 00:00:00

  • Single crystal structural and absorption spectral characterizations of nitric oxide synthase complexed with N(omega)-hydroxy-L-arginine and diatomic ligands.

    abstract::The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...


    pub_type: 杂志文章


    authors: Doukov T,Li H,Soltis M,Poulos TL

    更新日期:2009-11-03 00:00:00

  • Characterization of the reaction of L-serine and indole with Escherichia coli tryptophan synthase via rapid-scanning ultraviolet-visible spectroscopy.

    abstract::The pre-steady-state reaction of indole and L-serine with the alpha 2 beta 2 complex of Escherichia coli tryptophan synthase has been investigated under different premixing conditions with rapid-scanning stopped-flow (RSSF) UV-visible spectroscopy for the spectral range 300-550 nm. When alpha 2 beta 2 was mixed with i...


    pub_type: 杂志文章


    authors: Drewe WF Jr,Dunn MF

    更新日期:1986-05-06 00:00:00

  • The carboxyl-terminal region of factor IX is essential for its secretion.

    abstract::The carboxyl-terminal region of factor IX (residues 403-415) contains several natural mutations which result in mild to severe forms of hemophilia B. A battery of factor IX minigene expression vectors carrying various mutations in the C-terminal region were constructed and studied by transient expression assay using H...


    pub_type: 杂志文章


    authors: Kurachi S,Pantazatos DP,Kurachi K

    更新日期:1997-04-08 00:00:00

  • Binding of calcium to anticoagulant protein S: role of the fourth EGF module.

    abstract::Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...


    pub_type: 杂志文章


    authors: Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

    更新日期:2006-09-05 00:00:00

  • Simulation-based prediction of phosphatidylinositol 4,5-bisphosphate binding to an ion channel.

    abstract::Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...


    pub_type: 杂志文章


    authors: Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

    更新日期:2013-01-15 00:00:00

  • Exploring the role and the binding affinity of a second zinc equivalent in B. cereus metallo-beta-lactamase.

    abstract::Metallo-beta-lactamases are a newly characterized family of zinc enzymes present in several pathogenic strains that represent an emerging clinical threat. Enzymes from different organisms exhibit an outstanding functional diversity, particularly in the metal ion requirements for activity. We have investigated the effe...


    pub_type: 杂志文章


    authors: Rasia RM,Vila AJ

    更新日期:2002-02-12 00:00:00

  • Phosphorylation-dependent sumoylation of estrogen-related receptor alpha1.

    abstract::We previously showed that estrogen-related receptor alpha1 (ERRalpha1) can compete with estrogen receptor alpha (ERalpha) for binding to estrogen response elements (EREs), repressing transcription in the mammary carcinoma cell line MCF-7. Given that ERRalpha1 can function in the absence of ligands and exists as a phos...


    pub_type: 杂志文章


    authors: Vu EH,Kraus RJ,Mertz JE

    更新日期:2007-08-28 00:00:00

  • Mutational analysis of C-lobe ligands of human serum transferrin: insights into the mechanism of iron release.

    abstract::Each homologous lobe of human serum transferrin (hTF) has one Fe(3+) ion bound by an aspartic acid, a histidine, two tyrosine residues, and two oxygens from the synergistic anion, carbonate. Extensive characterization of these ligands in the N-terminal lobe has been carried out. Despite sharing the same set of ligands...


    pub_type: 杂志文章


    authors: Mason AB,Halbrooks PJ,James NG,Connolly SA,Larouche JR,Smith VC,MacGillivray RT,Chasteen ND

    更新日期:2005-06-07 00:00:00

  • A study of protein-sodium dodecyl sulfate complexes by transient electric birefringence.

    abstract::The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...


    pub_type: 杂志文章


    authors: Wright AK,Thompson MR,Miller RL

    更新日期:1975-07-15 00:00:00

  • Elastin binds to a multifunctional 67-kilodalton peripheral membrane protein.

    abstract::Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...


    pub_type: 杂志文章


    authors: Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

    更新日期:1989-05-02 00:00:00

  • A new variant of the anion transport protein in human erythrocytes.

    abstract::The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...


    pub_type: 杂志文章


    authors: Hsu L,Morrison M

    更新日期:1985-06-18 00:00:00

  • A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolase.

    abstract::Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoimidazole-4-carboxamide ribonucleotide (FAICAR) to IMP in the final step of de novo purine biosynthesis. Two major types of this enzyme have been discovered to date: PurH in Bacteria and Eukarya and PurO in Archaea. The structure of ...


    pub_type: 杂志文章


    authors: Kang YN,Tran A,White RH,Ealick SE

    更新日期:2007-05-01 00:00:00

  • Modification of ribonucleic acid by vitamin B6. 1. Specific interaction of pyridoxal 5'-phosphate with transfer ribonucleic acid.

    abstract::Whole tRNA preparation obtained from a human cell line (HT-29) of colon carcinoma and purified specific Escherichia coli tRNA were reacted with pyridoxal 5'-phosphate, reduced by sodium borohydride and digested with RNase A and snake venom phosphodiesterase. Two-dimensional chromatography of the pyridoxal 5'-phosphate...


    pub_type: 杂志文章


    authors: Kopelovich L,Wolfe G

    更新日期:1977-08-09 00:00:00

  • Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX.

    abstract::VanX, one of the five proteins required for the vancomycin-resistant phenotype in clinically pathogenic Enterococci, is a zinc-containing d-Ala-d-Ala dipeptidase. To identify potential zinc ligands and begin defining the active site residues, we have mutated the 2 cysteine, 5 histidine, and 4 of the 28 aspartate and g...


    pub_type: 杂志文章


    authors: McCafferty DG,Lessard IA,Walsh CT

    更新日期:1997-08-26 00:00:00

  • Characterization of inward-rectifier K+ channel inhibition by antiarrhythmic piperazine.

    abstract::Strong inward-rectifier K(+) (Kir) channels play a significant role in shaping the cardiac action potential: they help produce its long plateau and accelerate its rate of repolarization. Consequently, genetic deletion of the gene encoding the strongly rectifying K(+) channel IRK1 (Kir2.1) prolongs the cardiac action p...


    pub_type: 杂志文章


    authors: Xu Y,Lu Z

    更新日期:2004-12-14 00:00:00

  • A new omega-conotoxin that targets N-type voltage-sensitive calcium channels with unusual specificity.

    abstract::A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, ...


    pub_type: 杂志文章


    authors: Favreau P,Gilles N,Lamthanh H,Bournaud R,Shimahara T,Bouet F,Laboute P,Letourneux Y,Ménez A,Molgó J,Le Gall F

    更新日期:2001-12-04 00:00:00

  • Pyridoxamine-pyruvate transaminase. 1. Determination of the active site stoichiometry and the pH dependence of the dissociation constant for 5'-deoxypyridoxal.

    abstract::Spectrophotometric titration of pyridoxamine-pyruvate transaminase (EC with pyridoxal at pH 7.15 gives four equivalent binding sites per tetramer. The pH dependence of the equilibrium constant for the association of 5'-deoxypyridoxal with the active site lysine residue was determined spectrophotometrically. ...


    pub_type: 杂志文章


    authors: Gilmer PJ,McIntire WS,Kirsch JF

    更新日期:1977-11-29 00:00:00

  • Organellar Na+/H+ exchangers: novel players in organelle pH regulation and their emerging functions.

    abstract::Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...


    pub_type: 杂志文章,评审


    authors: Ohgaki R,van IJzendoorn SC,Matsushita M,Hoekstra D,Kanazawa H

    更新日期:2011-02-01 00:00:00

  • Picosecond tryptophan fluorescence of thioredoxin: evidence for discrete species in slow exchange.

    abstract::The steady-state tryptophan fluorescence and time-resolved tryptophan fluorescence of Escherichia coli thioredoxin, calf thymus thioredoxin, and yeast thioredoxin have been studied. In all proteins, the tryptophan residues undergo strong static and dynamic quenching, probably due to charge-transfer interactions with t...


    pub_type: 杂志文章


    authors: Mérola F,Rigler R,Holmgren A,Brochon JC

    更新日期:1989-04-18 00:00:00

  • Conformational changes in rhodopsin probed by surface plasmon resonance spectroscopy.

    abstract::Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. The magnitude of the SPR spectral changes caused by light varies with pH in a manner par...


    pub_type: 杂志文章


    authors: Salamon Z,Wang Y,Brown MF,Macleod HA,Tollin G

    更新日期:1994-11-22 00:00:00

  • Dissecting the catalytic mechanism of betaine-homocysteine S-methyltransferase by use of intrinsic tryptophan fluorescence and site-directed mutagenesis.

    abstract::Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...


    pub_type: 杂志文章


    authors: Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

    更新日期:2004-05-11 00:00:00

  • KappaM-conotoxin RIIIK, structural and functional novelty in a K+ channel antagonist.

    abstract::Venomous organisms have evolved a variety of structurally diverse peptide neurotoxins that target ion channels. Despite the lack of any obvious structural homology, unrelated toxins that interact with voltage-activated K(+) channels share a dyad motif composed of a lysine and a hydrophobic amino acid residue, usually ...


    pub_type: 杂志文章


    authors: Al-Sabi A,Lennartz D,Ferber M,Gulyas J,Rivier JE,Olivera BM,Carlomagno T,Terlau H

    更新日期:2004-07-13 00:00:00

  • Discovery and characterization of a substrate selective p38alpha inhibitor.

    abstract::A novel inhibitor of p38 mitogen-activated protein kinase (p38), CMPD1, identified by high-throughput screening, is characterized herein. Unlike the p38 inhibitors described previously, this inhibitor is substrate selective and noncompetitive with ATP. In steady-state kinetics experiments, CMPD1 was observed to preven...


    pub_type: 杂志文章


    authors: Davidson W,Frego L,Peet GW,Kroe RR,Labadia ME,Lukas SM,Snow RJ,Jakes S,Grygon CA,Pargellis C,Werneburg BG

    更新日期:2004-09-21 00:00:00

  • Circular dichroism and UV resonance raman study of the impact of alcohols on the Gibbs free energy landscape of an alpha-helical peptide.

    abstract::We used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran Psi-coordinate of a mainly poly-Ala peptide, AP with an AAAAA(AAARA)(3)A sequence. 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-heli...


    pub_type: 杂志文章


    authors: Xiong K,Asher SA

    更新日期:2010-04-20 00:00:00

  • Three small ribooligonucleotides with specific arginine sites.

    abstract::Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge...


    pub_type: 杂志文章


    authors: Connell GJ,Illangesekare M,Yarus M

    更新日期:1993-06-01 00:00:00

  • Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate.

    abstract::MantATP [2'(3')-O-(-N-methylanthraniloyl)-adenosine 5'-triphosphate] was employed as a fluorescence probe of the nucleotide-binding sites of dynein from sea urchin sperm flagella. MantATP binds specifically with enhanced fluorescence (approximately 2.2-fold), homogeneous lifetime (8.4 ns), and high anisotropy (r appro...


    pub_type: 杂志文章


    authors: Mocz G,Helms MK,Jameson DM,Gibbons IR

    更新日期:1998-07-07 00:00:00

  • Directed evolution of polymerases to accept nucleotides with nonstandard hydrogen bond patterns.

    abstract::Artificial genetic systems have been developed by synthetic biologists over the past two decades to include additional nucleotides that form additional nucleobase pairs independent of the standard T:A and C:G pairs. Their use in various tools to detect and analyze DNA and RNA requires polymerases that synthesize duple...


    pub_type: 杂志文章


    authors: Laos R,Shaw R,Leal NA,Gaucher E,Benner S

    更新日期:2013-08-06 00:00:00