Local and global dynamics of the G protein-coupled receptor CXCR1.

Abstract:

:The local and global dynamics of the chemokine receptor CXCR1 are characterized using a combination of solution NMR and solid-state NMR experiments. In isotropic bicelles (q = 0.1), only 13% of the expected number of backbone amide resonances is observed in (1)H/(15)N HSQC solution NMR spectra of uniformly (15)N-labeled samples; extensive deuteration and the use of TROSY made little difference in the 800 MHz spectra. The limited number of observed amide signals is ascribed to mobile backbone sites and assigned to specific residues in the protein; 19 of the signals are from residues at the N-terminus and 25 from residues at the C-terminus. The solution NMR spectra display no evidence of local backbone motions from residues in the transmembrane helices or interhelical loops of CXCR1. This finding is reinforced by comparisons of solid-state NMR spectra of both magnetically aligned and unoriented bilayers containing either full-length or doubly N- and C-terminal truncated CXCR1 constructs. CXCR1 undergoes rapid rotational diffusion about the normal of liquid crystalline phospholipid bilayers; reductions in the frequency span and a change to axial symmetry are observed for both carbonyl carbon and amide nitrogen chemical shift powder patterns of unoriented samples containing (13)C- and (15)N-labeled CXCR1. In contrast, when the phospholipids are in the gel phase, CXCR1 does not undergo rapid global reorientation on the 10(4) Hz time scale defined by the carbonyl carbon and amide nitrogen chemical shift powder patterns.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Park SH,Casagrande F,Das BB,Albrecht L,Chu M,Opella SJ

doi

10.1021/bi101568j

subject

Has Abstract

pub_date

2011-03-29 00:00:00

pages

2371-80

issue

12

eissn

0006-2960

issn

1520-4995

journal_volume

50

pub_type

杂志文章
  • Nucleosome recognition by the Piccolo NuA4 histone acetyltransferase complex.

    abstract::The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi602366n

    authors: Berndsen CE,Selleck W,McBryant SJ,Hansen JC,Tan S,Denu JM

    更新日期:2007-02-27 00:00:00

  • Electronic structure studies of the adenosylcobalamin cofactor in glutamate mutase.

    abstract::Glutamate mutase (GM) is a cobalamin-dependent enzyme that catalyzes the reversible interconversion of L-glutamate and L-threo-3-methylaspartate via a radical-based mechanism. To initiate catalysis, the 5'-deoxyadenosylcobalamin (AdoCbl) cofactor's Co-C bond is cleaved homolytically to generate an adenosyl radical and...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi051094y

    authors: Brooks AJ,Fox CC,Marsh EN,Vlasie M,Banerjee R,Brunold TC

    更新日期:2005-11-22 00:00:00

  • Affinity labeling of Escherichia coli phenylalanyl-tRNA synthetase at the binding site for tRNAPhe.

    abstract::Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00391a033

    authors: Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

    更新日期:1987-08-25 00:00:00

  • Effect of pressure on deuterium isotope effects of yeast alcohol dehydrogenase: evidence for mechanical models of catalysis.

    abstract::Moderate pressure accelerates hydride transfer catalyzed by yeast alcohol dehydrogenase, indicative of a large negative volume of activation [Cho and Northrop (1999) Biochemistry 38, 7470-7475]. A comparison of the effects of pressure on the oxidation of normal versus dideuteriobenzyl alcohol generates a monophasic de...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi992537z

    authors: Northrop DB,Cho YK

    更新日期:2000-03-07 00:00:00

  • Characterization of human calcitonin fibrillation in aqueous urea solution by 1H NMR spectroscopy.

    abstract::The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi961013l

    authors: Kanaori K,Nosaka AY

    更新日期:1996-10-01 00:00:00

  • Evidence for targeted gene delivery to Hep G2 hepatoma cells in vitro.

    abstract::We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00403a008

    authors: Wu GY,Wu CH

    更新日期:1988-02-09 00:00:00

  • Evidence of random structural features in the heparin polymer.

    abstract::The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydisper...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00347a045

    authors: Linhardt RJ,Merchant ZM,Rice KG,Kim YS,Fitzgerald GL,Grant AC,Langer R

    更新日期:1985-12-17 00:00:00

  • Replacement of a conserved proline and the alkaline conformational change in iso-2-cytochrome c.

    abstract::Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00451a043

    authors: Nall BT,Zuniga EH,White TB,Wood LC,Ramdas L

    更新日期:1989-12-12 00:00:00

  • Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.

    abstract::We have determined the fidelity of in vitro DNA synthesis catalyzed at high temperature by the DNA polymerase from the thermophilic bacterium Thermus aquaticus. Using a DNA substrate that contains a 3'-OH terminal mismatch, we demonstrate that the purified polymerase lacks detectable exonucleolytic proofreading activi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00416a027

    authors: Tindall KR,Kunkel TA

    更新日期:1988-08-09 00:00:00

  • Interactions of poly(N epsilon, N epsilon, N epsilon-trimethyllysine) and poly(N delta, N delta, N delta-trimethylornithine) with polynucleotides: salt dissociation and thermal denaturation.

    abstract::The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00555a020

    authors: Granados EN,Bello J

    更新日期:1980-07-08 00:00:00

  • Effect of solvent diffusion on the apomyoglobin-water interface.

    abstract::Few techniques can identify interactions between proteins and individual water molecules when the protein is in solution. The present work has sought to bridge the gap between the molecular level studies and the search for a physical property of the solution (bathing the proteins) that would regulate the protein hydra...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi012210+

    authors: Twist C,Royer C,Alpert B

    更新日期:2002-08-13 00:00:00

  • Picosecond absorption studies on rhodopsin and isorhodopsin in detergent and native membrane.

    abstract::Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00312a008

    authors: Rudzki JE,Peters KS

    更新日期:1984-08-14 00:00:00

  • Restriction endonuclease EcoRI alters the enantiomeric preference of chiral metallointercalators for DNA: an illustration of a protein-induced DNA conformational change.

    abstract::A conformational change in the DNA plasmid ColE1 appears to occur upon specific binding of the restriction endonuclease EcoRI. Enzyme association alters the chiral discrimination found in binding metallointercalators to DNA sites. The complexes tris(1,10-phenanthroline)ruthenium(II), Ru(phen)3(2+), tris(4,7-diphenyl-1...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00356a053

    authors: Barton JK,Paranawithana SR

    更新日期:1986-04-22 00:00:00

  • Stable DNA-protein cross-links are products of DNA charge transport in a nucleosome core particle.

    abstract::DNA-protein cross-links (DPCs) in nucleosome core particles (NCPs), the fundamental building block of chromatin, arise during times of cellular oxidative stress. These lesions are expected to be detrimental to the cell due to interference with processes like chromatin remodeling, transcription, DNA replication, and ep...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi700475b

    authors: Bjorklund CC,Davis WB

    更新日期:2007-09-25 00:00:00

  • Faithful initiation of ribosomal RNA transcription from cloned DNA by purified RNA polymerase I.

    abstract::A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00313a025

    authors: Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

    更新日期:1984-08-28 00:00:00

  • Helix 8 and helix 10 are involved in substrate recognition in the rat monocarboxylate transporter MCT1.

    abstract::Transport of lactate, pyruvate, and the ketone bodies, acetoacetate and beta-hydroxybutyrate, is mediated in many mammalian cells by the monocarboxylate transporter MCT1. To be accepted as a substrate, a carboxyl group and an unpolar side chain are necessary. Site-directed mutagenesis of the rat MCT1 was used to ident...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi990973f

    authors: Rahman B,Schneider HP,Bröer A,Deitmer JW,Bröer S

    更新日期:1999-08-31 00:00:00

  • Solution structure of the biologically relevant G-quadruplex element in the human c-MYC promoter. Implications for G-quadruplex stabilization.

    abstract::The nuclease hypersensitivity element III(1) (NHE III(1)) of the c-MYC promoter strongly controls the transcriptional activity of the c-MYC oncogene. The purine-rich strand of the NHE III(1) element has been shown to be a silencer element for c-MYC transcription upon formation of a G-quadruplex structure. We have dete...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048242p

    authors: Ambrus A,Chen D,Dai J,Jones RA,Yang D

    更新日期:2005-02-15 00:00:00

  • Deep-UV resonance Raman analysis of the Rhodobacter capsulatus cytochrome bc₁complex reveals a potential marker for the transmembrane peptide backbone.

    abstract::Classical strategies for structure analysis of proteins interacting with a lipid phase typically correlate ensemble secondary structure content measurements with changes in the spectroscopic responses of localized aromatic residues or reporter molecules to map regional solvent environments. Deep-UV resonance Raman (DU...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi200596w

    authors: Halsey CM,Oshokoya OO,Jiji RD,Cooley JW

    更新日期:2011-08-02 00:00:00

  • Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark.

    abstract::The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00660a033

    authors: Holowka DA,Cathou RE

    更新日期:1976-07-27 00:00:00

  • Structural and spectroscopic studies of the copper site of stellacyanin.

    abstract::The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00001a026

    authors: Strange RW,Reinhammar B,Murphy LM,Hasnain SS

    更新日期:1995-01-10 00:00:00

  • Importance of asparagine-61 and asparagine-109 to the angiogenic activity of human angiogenin.

    abstract::Two distinct regions of angiogenin are critical for angiogenic activity: a catalytic site capable of cleaving RNA and a noncatalytic site, encompassing residues 60-68, which may bind to an endothelial cell-surface receptor [Hallahan, T. W., Shapiro, R., & Vallee, B. L. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 2222-222...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00149a036

    authors: Hallahan TW,Shapiro R,Strydom DJ,Vallee BL

    更新日期:1992-09-01 00:00:00

  • cGMP phosphodiesterase dependent light-induced scattering changes in suspensions of retinal disc membranes.

    abstract::Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00121a039

    authors: Bennett N,Clerc A

    更新日期:1992-02-18 00:00:00

  • Importance of the region around glycine-338 for the activity of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system.

    abstract::The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952052k

    authors: Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

    更新日期:1996-01-09 00:00:00

  • Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry.

    abstract::The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00480a026

    authors: Westrick JA,Peters KS,Ropp JD,Sligar SG

    更新日期:1990-07-17 00:00:00

  • Evidence for two spectroscopically different dimers of light-harvesting complex I from green plants.

    abstract::A preparation consisting of isolated dimeric peripheral antenna complexes from green plant photosystem I (light-harvesting complex I or LHCI) has been characterized by means of (polarized) steady-state absorption and fluorescence spectroscopy at low temperatures. We show that this preparation can be described reasonab...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0007369

    authors: Ihalainen JA,Gobets B,Sznee K,Brazzoli M,Croce R,Bassi R,van Grondelle R,Korppi-Tommola JE,Dekker JP

    更新日期:2000-07-25 00:00:00

  • Sulfated glycoproteins, glycolipids, and glycosaminoglycans from synaptic plasma and myelin membranes: isolation and characterization of sulfated glycopeptides.

    abstract::In this report we provide biochemical evidence that a highly purified synaptic plasma membrane fraction derived from rat brain, after intraventricular injection of 35S-labeled sodium sulfate, is enriched in a number of large sulfated glycoproteins compared with a purified myelin fraction studied concurrently. A fracti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00670a005

    authors: Simpson DL,Thorne DR,Loch HH

    更新日期:1976-12-14 00:00:00

  • Characterization of the collagens synthesized by Chinese hamster ovary cells. Effect of colcemid and dibutyryladenosine cyclic monophosphate.

    abstract::The collagens synthesized by Chinese hamster ovary cells have been isolated and characterized. Although these cells produce very small amounts of collagen, at least five distinct collagenous chains could be identified from radiolabeled media and cell extracts after limited pepsin digestion. Two chains were characteriz...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00262a031

    authors: Limeback H,Sodek J,Aubin J

    更新日期:1982-09-14 00:00:00

  • Domain characteristics of the carboxyl-terminal fragment 206-316 of thermolysin: immunochemical studies.

    abstract::The extent of nativeness of the stable conformation of the thermolysin fragment containing the carboxyl-terminal third of the protein (from residues 206 to 316, denoted fragment FII) was examined by its immunogenic and antigenic characteristics. Antisera elicited in rabbits by either intact thermolysin or fragment FII...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00538a007

    authors: Vita C,Fontana A,Chaiken IM

    更新日期:1982-04-27 00:00:00

  • Assembly of a [2Fe-2S]2+ cluster in a molecular variant of Clostridium pasteurianum rubredoxin.

    abstract::The rubredoxin from Clostridium pasteurianum contains a single iron atom bound to the polypeptide chain by cysteines 6, 9, 39, and 42. The C42A variant of this protein has been prepared by site-directed mutagenesis and heterologous expression of the gene in Escherichia coli. The mutated protein was found to contain an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971775w

    authors: Meyer J,Gagnon J,Gaillard J,Lutz M,Achim C,Münck E,Pétillot Y,Colangelo CM,Scott RA

    更新日期:1997-10-28 00:00:00

  • SNX-325, a novel calcium antagonist from the spider Segestria florentina.

    abstract::A novel selective calcium channel antagonist peptide, SNX-325, has been isolated from the venom of the spider Segestria florentina. The peptide was isolated using as bioassays the displacement of radioiodinated omega-conopeptide SNX-230 (MVIIC) from rat brain synaptosomal membranes, as well as the inhibition of the ba...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00026a015

    authors: Newcomb R,Palma A,Fox J,Gaur S,Lau K,Chung D,Cong R,Bell JR,Horne B,Nadasdi L

    更新日期:1995-07-04 00:00:00