Ginsenosides are potent and selective inhibitors of some calmodulin-dependent phosphodiesterase isozymes.

abstract：:Cytochrome P450 3A4 is a drug-metabolizing enzyme of extraordinarily broad substrate specificity. This quality imparts upon the enzyme special importance in understanding its determinants of activity and substrate recognition. Limited successes in P450 3A4 active-site structure studies have been achieved by use of mec...

journal_title：Biochemistry

authors： Gartner CA,Wen B,Wan J,Becker RS,Jones G 2nd,Gygi SP,Nelson SD

更新日期：2005-02-15 00:00:00

abstract：:Central to the pathology of Alzheimer's disease (AD) is the profuse accumulation of amyloid-beta (Abeta) peptides in the brain of affected individuals, and several amyloid precursor protein (APP) transgenic (Tg) mice models have been created to mimic Abeta deposition. Among these, the PDAPP Tg mice carrying the famili...

journal_title：Biochemistry

authors： Esh C,Patton L,Kalback W,Kokjohn TA,Lopez J,Brune D,Newell AJ,Beach T,Schenk D,Games D,Paul S,Bales K,Ghetti B,Castaño EM,Roher AE

更新日期：2005-10-25 00:00:00

abstract：:The effects of mutation of the conserved serine92 residue to alanine, valine, and leucine in pig myoglobin have been determined. In myoglobin crystal structures, the hydroxyl group of serine92 is within hydrogen-bonding distance of the N delta-H of histidine93, whose N epsilon coordinates the iron atom of the heme pro...

journal_title：Biochemistry

authors： Smerdon SJ,Krzywda S,Wilkinson AJ,Brantley RE Jr,Carver TE,Hargrove MS,Olson JS

更新日期：1993-05-18 00:00:00

abstract：:By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...

journal_title：Biochemistry

authors： Tsunenaga M,Goto Y,Kawata Y,Hamaguchi K

更新日期：1987-09-22 00:00:00

abstract：:The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...

journal_title：Biochemistry

authors： Kohno T,Kobayashi K,Maeda T,Sato K,Takashima A

更新日期：1996-12-17 00:00:00

abstract：:Paracoccus denitrificans synthesizes an inducible, periplasmic, blue copper protein [Husain, M., & Davidson, V.L. (1985) J. Biol. Chem. 260, 14626-14629] that can be classified as an amicyanin on the basis of its ability to accept electrons from methylamine dehydrogenase. The amino acid composition and sequence of the...

journal_title：Biochemistry

authors： Husain M,Davidson VL,Smith AJ

更新日期：1986-05-06 00:00:00

abstract：:We have performed a kinetic and thermodynamic analysis of 3D(pol) derivatives containing substitutions in the ribose-binding pocket with ATP analogues containing correct and incorrect sugar configurations. We find that Asp-238, a residue in structural motif A that is conserved in all RNA-dependent RNA polymerases, is ...

journal_title：Biochemistry

authors： Gohara DW,Arnold JJ,Cameron CE

更新日期：2004-05-11 00:00:00

abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

journal_title：Biochemistry

authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

更新日期：2001-08-21 00:00:00

abstract：:The kinetic parameters (kpol, Kd app) for all possible correct and incorrect pairing between the A, T, G, and C bases were determined for wild-type (WT) rat DNA polymerase beta (pol beta) and the R283A mutant under pre-steady-state kinetic assay conditions. The base substitution fidelities of these two proteins were t...

journal_title：Biochemistry

authors： Ahn J,Werneburg BG,Tsai MD

更新日期：1997-02-04 00:00:00

abstract：:Electron-transfer reactions following the formation of P700(+)A1- have been studied in isolated Photosystem I complexes from Synechococcus elongatus between 300 and 5 K by flash absorption spectroscopy. (1) In the range from 300 to 200 K, A1- is reoxidized by electron transfer to the iron-sulfur cluster FX. The rate s...

journal_title：Biochemistry

authors： Schlodder E,Falkenberg K,Gergeleit M,Brettel K

更新日期：1998-06-30 00:00:00

abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...

journal_title：Biochemistry

authors： Zhu Z,Becklin RR,Desiderio DM,Dalton JT

更新日期：2001-09-11 00:00:00

abstract：:A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3 mi...

journal_title：Biochemistry

authors： Kusumi A,Tsuji A,Murata M,Sako Y,Yoshizawa AC,Kagiwada S,Hayakawa T,Ohnishi S

更新日期：1991-07-02 00:00:00

abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...

journal_title：Biochemistry

authors： Davlieva M,Wu C,Zhou Y,Arias CA,Shamoo Y

更新日期：2018-12-11 00:00:00

abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

journal_title：Biochemistry

authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

更新日期：2005-04-26 00:00:00

abstract：:The fluorescence intensity of the two flavin prosthetic groups, FMN and FAD, in neuronal nitric oxide synthase (nNOS) was found to decay highly nonexponentially, being best described by four fluorescence lifetimes. This excited state heterogeneity is the result of multiple flavin quenching sites which are due to sever...

journal_title：Biochemistry

authors： Brunner K,Tortschanoff A,Hemmens B,Andrew PJ,Mayer B,Kungl AJ

更新日期：1998-12-15 00:00:00

abstract：:At pH 5.5, binding of bleomycin relaxed supercoiled ColE1 DNA without breaking it. Binding of tripeptide S, a fragment of the drug containing the bithiazole rings, also relaxed and then recoiled supercoiled DNA, at pH 5.5 and at pH 8.0, where bleomycin is normally active. The unwinding angle was 12 degrees. Both compo...

journal_title：Biochemistry

authors： Povirk LF,Hogan M,Dattagupta N

更新日期：1979-01-09 00:00:00

abstract：:Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...

journal_title：Biochemistry

authors： Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

更新日期：1999-03-02 00:00:00

abstract：:The stereochemical course of the phospho group transfer catalyzed by mutant (S102C) alkaline phosphatase from Escherichia coli was investigated by using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from 4-nitrophenyl (Rp)-[16O, 17O, 18O]phosphate to (S)-propane-1,2-diol occurs with overall retenti...

journal_title：Biochemistry

authors： Butler-Ransohoff JE,Kendall DA,Freeman S,Knowles JR,Kaiser ET

更新日期：1988-06-28 00:00:00

abstract：:Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterizat...

journal_title：Biochemistry

authors： Balentien E,Han JH,Thomas HG,Wen DZ,Samantha AK,Zachariae CO,Griffin PR,Brachmann R,Wong WL,Matsushima K

更新日期：1990-11-06 00:00:00

abstract：:3-Deoxy-d- manno-2-octulosonate-8-phosphate (KDO8P) synthase catalyzes the first step of lipopolysaccharide biosynthesis, namely condensation of phosphoenolpyruvate (PEP) with arabinose 5-phosphate (A5P), to produce KDO8P. We have characterized Campylobacter jejuni KDO8P synthase and its inhibition by KDO8P oxime. It ...

journal_title：Biochemistry

authors： Gama SR,Balachandran N,Berti PJ

更新日期：2018-09-11 00:00:00

abstract：:We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...

journal_title：Biochemistry

authors： Watson DK,Moudrianakis EN

更新日期：1982-01-19 00:00:00

abstract：:A high-resolution solution conformation of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA, GCCGSYPNAACHPCSCKDROSYCGQ-NH2, has been determined by two-dimensional 1H NMR methods and distance geometry calculations. The total of 324 NOE-derived interproton distance restraints including 33 long-range NOE restraints a...

journal_title：Biochemistry

authors： Han KH,Hwang KJ,Kim SM,Kim SK,Gray WR,Olivera BM,Rivier J,Shon KJ

更新日期：1997-02-18 00:00:00

abstract：:Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...

journal_title：Biochemistry

authors： Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-03-17 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00

abstract：:Using proton magnetic resonance, we have investigated imino and amino proton exchange in the Z form of the four oligomers d(Cbr8GCGCbr8G), d(CGm5CGCG), d(CG)6, and d(CG)12. In the latter two oligomers, all five exchangeable protons have been assigned. We find that proton acceptors such as NH3 or the basic form of Tris...

journal_title：Biochemistry

authors： Kochoyan M,Leroy JL,Guéron M

更新日期：1990-05-22 00:00:00

abstract：:Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...

journal_title：Biochemistry

authors： Petrillo T,O'Donohoe CA,Howe N,Malthouse JP

更新日期：2012-08-07 00:00:00

abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

journal_title：Biochemistry

authors： Richardson CJ,First EA

更新日期：2016-03-15 00:00:00

abstract：:We found that peroxisomal lignoceroyl-CoA ligase, like palmitoyl-CoA ligase, is present in the peroxisomal membrane whereas the peroxisomal beta-oxidation enzyme system is localized in the matrix. To further define the role of peroxisomal acyl-CoA ligases (membrane component) in providing acyl-CoA for peroxisomal beta...

journal_title：Biochemistry

authors： Lazo O,Contreras M,Singh I

更新日期：1990-04-24 00:00:00

abstract：:Reducing sugars such as glucose react nonenzymatically with protein amino groups to initiate a posttranslational modification process known as advanced glycosylation. Nucleotide bases also participate in advanced glycosylation reactions, producing DNA-linked advanced glycosylation endproducts (AGEs) that cause mutatio...

journal_title：Biochemistry

authors： Papoulis A,al-Abed Y,Bucala R

更新日期：1995-01-17 00:00:00

abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...

journal_title：Biochemistry

authors： Allocatelli CT,Cutruzzolà F,Brancaccio A,Brunori M,Qin J,La Mar GN

更新日期：1993-06-15 00:00:00