Stereochemistry of phospho group transfer catalyzed by a mutant alkaline phosphatase.


:The stereochemical course of the phospho group transfer catalyzed by mutant (S102C) alkaline phosphatase from Escherichia coli was investigated by using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from 4-nitrophenyl (Rp)-[16O, 17O, 18O]phosphate to (S)-propane-1,2-diol occurs with overall retention of configuration at phosphorus. This result is consistent with the view that the hydrolysis of substrates by this mutant enzyme proceeds by way of a covalent phosphoenzyme intermediate in the same manner as the wild-type alkaline phosphatase.






Butler-Ransohoff JE,Kendall DA,Freeman S,Knowles JR,Kaiser ET




Has Abstract


1988-06-28 00:00:00












  • Roles of cysteines in human lecithin:cholesterol acyltransferase.

    abstract::Human lecithin:cholesterol acyltransferase (LCAT, E.C. is a serine-type esterase that contains six cysteines, two of which, Cys31 and Cys184, are free. The remaining cysteines form disulfide links. One of these is between Cys50 and Cys74 and the other is between Cys313 and Cys356. The cDNA of LCAT and mutants...


    pub_type: 杂志文章


    authors: Qu SJ,Fan HZ,Blanco-Vaca F,Pownall HJ

    更新日期:1993-03-30 00:00:00

  • High-pressure denaturation of staphylococcal nuclease proline-to-glycine substitution mutants.

    abstract::Our recently reported pressure-jump relaxation kinetics experiments on staphylococcal nuclease folding and unfolding [Vidugiris et al. (1995) Biochemistry 34, 4909] demonstrated that both transitions exhibit positive activation volumes, with that of folding being much larger than that of unfolding. Thus high pressure ...


    pub_type: 杂志文章


    authors: Vidugiris GJ,Truckses DM,Markley JL,Royer CA

    更新日期:1996-03-26 00:00:00

  • Thermodynamic cycle analysis and inhibitor design against beta-lactamase.

    abstract::Beta-lactamases are the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and cephalosporins. Transition-state analogues that bind to the enzymes with nanomolar affinities have been introduced in an effort to reverse the resistance conferred by these enzymes. To understand the or...


    pub_type: 杂志文章


    authors: Roth TA,Minasov G,Morandi S,Prati F,Shoichet BK

    更新日期:2003-12-16 00:00:00

  • Apolipoprotein E expression in Y1 adrenal cells is associated with increased intracellular cholesterol content and reduced free cholesterol efflux.

    abstract::The expression of apoE mRNA in the adrenal gland is inversely correlated to steroidogenesis and directly correlated to the level of cholesteryl ester stores. To further investigate the relationship between apoE and cellular cholesterol homeostasis, several parameters of cholesterol metabolism in the murine Y1 adrenal ...


    pub_type: 杂志文章


    authors: Prack MM,Rothblat GH,Erickson SK,Reyland ME,Williams DL

    更新日期:1994-05-03 00:00:00

  • Electron spin echo modulation and nuclear relaxation studies of staphylococcal nuclease and its metal-coordinating mutants.

    abstract::Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...


    pub_type: 杂志文章


    authors: Serpersu EH,McCracken J,Peisach J,Mildvan AS

    更新日期:1988-10-18 00:00:00

  • The intrinsic protein flexibility of endogenous protease inhibitor TIMP-1 controls its binding interface and affects its function.

    abstract::Protein flexibility is thought to play key roles in numerous biological processes, including antibody affinity maturation, signal transduction, and enzyme catalysis, yet only limited information is available regarding the molecular details linking protein dynamics with function. A single point mutation at the distal s...


    pub_type: 杂志文章


    authors: Grossman M,Tworowski D,Dym O,Lee MH,Levy Y,Murphy G,Sagi I

    更新日期:2010-07-27 00:00:00

  • Role of the covalent flavin linkage in monomeric sarcosine oxidase.

    abstract::Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...


    pub_type: 杂志文章


    authors: Hassan-Abdallah A,Zhao G,Jorns MS

    更新日期:2006-08-08 00:00:00

  • The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid.

    abstract::When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNA(Phe), a single tRNA body was misacylated with 13 different amino acids. The affinities of these misacylated tRNAs for Thermus thermophilus elongation factor Tu (EF-Tu).GTP were determined using a rib...


    pub_type: 杂志文章


    authors: Dale T,Sanderson LE,Uhlenbeck OC

    更新日期:2004-05-25 00:00:00

  • A phosphotransferase activity of the Bacillus subtilis sporulation protein Spo0F that employs phosphoramidate substrates.

    abstract::Transient phosphorylation at an aspartate residue on the Spo0F protein is a central step in the phosphorelay signal transduction pathway controlling sporulation in Bacilli. The response regulator Spo0F-P is stable to hydrolysis (t1/2 > 24 h at 23 degrees C in the absence of Mg2+), allowing the use of nondenaturing PAG...


    pub_type: 杂志文章


    authors: Zapf JW,Hoch JA,Whiteley JM

    更新日期:1996-03-05 00:00:00

  • Insights into the mechanism of Pseudomonas dacunhae aspartate beta-decarboxylase from rapid-scanning stopped-flow kinetics.

    abstract::The mechanism of wild-type and R37A mutant Pseudomonas dacunhae aspartate beta-decarboxylase (ABDC) was studied by rapid-scanning stopped-flow spectrophotometry. Mixing wild-type ABDC with 50 mM disodium l-Asp resulted in the formation of a 325 nm absorption peak within the dead time of the stopped-flow instrument, li...


    pub_type: 杂志文章


    authors: Phillips RS,Lima S,Khristoforov R,Sudararaju B

    更新日期:2010-06-22 00:00:00

  • Mechanism of the PII-activated phosphatase activity of Escherichia coli NRII (NtrB): how the different domains of NRII collaborate to act as a phosphatase.

    abstract::The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...


    pub_type: 杂志文章


    authors: Pioszak AA,Ninfa AJ

    更新日期:2003-07-29 00:00:00

  • A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.

    abstract::Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...


    pub_type: 杂志文章


    authors: Pak ML,Lakowski TM,Thomas D,Vhuiyan MI,Hüsecken K,Frankel A

    更新日期:2011-09-27 00:00:00

  • Access to the active site of periplasmic nitrate reductase: insights from site-directed mutagenesis and zinc inhibition studies.

    abstract::The periplasmic nitrate reductase (NapAB), a member of the DMSO reductase superfamily, catalyzes the first step of the denitrification process in bacteria. In this heterodimer, a di-heme NapB subunit is associated to the catalytic NapA subunit that binds a [4Fe-4S] cluster and a bis(molybdopterin guanine dinucleotide)...


    pub_type: 杂志文章


    authors: Dementin S,Arnoux P,Frangioni B,Grosse S,Léger C,Burlat B,Guigliarelli B,Sabaty M,Pignol D

    更新日期:2007-08-28 00:00:00

  • Folding domains as functional tools in allosteric systems: a heme-dependent domain in hemoglobin beta subunits.

    abstract::We have investigated the denaturation by guanidine hydrochloride (Gdn X HCl), temperature, and pH of hemoglobin beta subunits and of the peptides beta (1-146), beta (56-146), and beta (1-55). The last peptide was insensitive to all of the three agents. In the other polypeptides denaturation by Gdn X HCl and temperatur...


    pub_type: 杂志文章


    authors: Franchi D,Fronticelli C,Bucci E

    更新日期:1982-11-23 00:00:00

  • Hummel-Dreyer gel chromatographic procedure as applied to ligand-mediated association.

    abstract::A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand bi...


    pub_type: 杂志文章


    authors: Cann JR,Hinman ND

    更新日期:1976-10-19 00:00:00

  • Biochemical properties of phosphatidylcholine-binding proteins that share common antigenic determinants with Fc gamma 2b receptor.

    abstract::Biochemical and immunological properties of biosynthetically radiolabeled phosphatidylcholine-(PC-) binding proteins were investigated. The PC-binding proteins were extracted from the detergent lysate of biosynthetically radiolabeled P388D1 cells by affinity chromatography on PC-Sepharose and filtered through a Sephad...


    pub_type: 杂志文章


    authors: Fernandez-Botran R,Suzuki T

    更新日期:1985-04-09 00:00:00

  • A mechanistic investigation of the C-terminal redox motif of thioredoxin reductase from Plasmodium falciparum.

    abstract::High-molecular mass thioredoxin reductases (TRs) are pyridine nucleotide disulfide oxidoreductases that catalyze the reduction of the disulfide bond of thioredoxin (Trx). Trx is responsible for reducing multiple protein disulfide targets in the cell. TRs utilize reduced β-nicotinamide adenine dinucleotide phosphate to...


    pub_type: 杂志文章


    authors: Snider GW,Dustin CM,Ruggles EL,Hondal RJ

    更新日期:2014-01-28 00:00:00

  • Substrate specificity of homogeneous monkeypox virus uracil-DNA glycosylase.

    abstract::Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...


    pub_type: 杂志文章


    authors: Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

    更新日期:2007-10-23 00:00:00

  • Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds as DNA-protein cross-linking agents: potential suicide DNA lesions.

    abstract::Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds having the generalized formula M(a)NH2(CH)4NH2M(b) are shown to form specific DNA lesions which can efficiently cross-link proteins to DNA. In this study, the homodinuclear case is represented by M(a) = M(b) = [cis-Pt(Cl2)-(NH3)] and the heterodinuclear...


    pub_type: 杂志文章


    authors: Van Houten B,Illenye S,Qu Y,Farrell N

    更新日期:1993-11-09 00:00:00

  • Solution structure of the first Sam domain of Odin and binding studies with the EphA2 receptor.

    abstract::The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...


    pub_type: 杂志文章


    authors: Mercurio FA,Marasco D,Pirone L,Pedone EM,Pellecchia M,Leone M

    更新日期:2012-03-13 00:00:00

  • Kinetic evidence for a monomer activation step in actin polymerization.

    abstract::We measured the time course of skeletal muscle actin polymerization at different actin concentrations. In 0.1 M KCl with 1 mM Mg2+, log/log plots of the rate of the early, slow phase of polymerization vs. actin concentration were linear with slopes from 1.0 to 1.3. Computer-assisted calculations of similar curves from...


    pub_type: 杂志文章


    authors: Cooper JA,Buhle EL Jr,Walker SB,Tsong TY,Pollard TD

    更新日期:1983-04-26 00:00:00

  • Identification of an ubinuclein 1 region required for stability and function of the human HIRA/UBN1/CABIN1/ASF1a histone H3.3 chaperone complex.

    abstract::The mammalian HIRA/UBN1/CABIN1/ASF1a (HUCA) histone chaperone complex deposits the histone H3 variant H3.3 into chromatin and is linked to gene activation, repression, and chromatin assembly in diverse cell contexts. We recently reported that a short N-terminal fragment of UBN1 containing amino acids 1-175 is necessar...


    pub_type: 杂志文章


    authors: Tang Y,Puri A,Ricketts MD,Rai TS,Hoffmann J,Hoi E,Adams PD,Schultz DC,Marmorstein R

    更新日期:2012-03-27 00:00:00

  • Kinetic mechanism of the GCN5-related chromosomal aminoglycoside acetyltransferase AAC(6')-Ii from Enterococcus faecium: evidence of dimer subunit cooperativity.

    abstract::The aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii from Enterococcus faecium is an important microbial resistance determinant and a member of the GCN5-related N-acetyltransferase (GNAT) superfamily. We report here the further characterization of this enzyme in terms of the kinetic mechanism of acetyl transfer and id...


    pub_type: 杂志文章


    authors: Draker KA,Northrop DB,Wright GD

    更新日期:2003-06-03 00:00:00

  • Uniform 13C isotope labeling of proteins with sodium acetate for NMR studies: application to human carbonic anhydrase II.

    abstract::Uniform double labeling of proteins for NMR studies can be prohibitively expensive, even with an efficient expression and purification scheme, due largely to the high cost of [13C6, 99%]glucose. We demonstrate here that uniformly (greater than 95%) 13C and 15N double-labeled proteins can be prepared for NMR structure/...


    pub_type: 杂志文章


    authors: Venters RA,Calderone TL,Spicer LD,Fierke CA

    更新日期:1991-05-07 00:00:00

  • Michler's hydrol blue: a sensitive probe for amyloid fibril detection.

    abstract::Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation ...


    pub_type: 杂志文章


    authors: Kitts CC,Beke-Somfai T,Nordén B

    更新日期:2011-05-03 00:00:00

  • Human low-molecular-weight salivary mucin expresses the sialyl lewisx determinant and has L-selectin ligand activity.

    abstract::Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...


    pub_type: 杂志文章


    authors: Prakobphol A,Thomsson KA,Hansson GC,Rosen SD,Singer MS,Phillips NJ,Medzihradszky KF,Burlingame AL,Leffler H,Fisher SJ

    更新日期:1998-04-07 00:00:00

  • Topological equilibria of ion channel peptides in oriented lipid bilayers revealed by 15N solid-state NMR spectroscopy.

    abstract::Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...


    pub_type: 杂志文章


    authors: Sudheendra US,Bechinger B

    更新日期:2005-09-13 00:00:00

  • GRP1 pleckstrin homology domain: activation parameters and novel search mechanism for rare target lipid.

    abstract::Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...


    pub_type: 杂志文章


    authors: Corbin JA,Dirkx RA,Falke JJ

    更新日期:2004-12-28 00:00:00

  • Molecular characterization of the catalytic domains of human complement serine protease C1r.

    abstract::Limited cleavages of human C1r by extrinsic proteases of various specificity (plasmin, elastase, chymotrypsin, thermolysin) yield dimeric associations of two globular domains, each comprised of the intact B chain disulfide linked to gamma, the C-terminal fragment of the A chain. These (gamma-B)2 domains, which are hom...


    pub_type: 杂志文章


    authors: Arlaud GJ,Gagnon J,Villiers CL,Colomb MG

    更新日期:1986-09-09 00:00:00

  • Direct evidence for GTP and GDP-Pi intermediates in microtubule assembly.

    abstract::Identification of the kinetic intermediates in GTP hydrolysis on microtubules and characterization of their assembly properties is essential in understanding microtubule dynamics. By using an improved glass filter assay that selectively traps microtubules with a dead time of 2 s and monitoring taxol-induced rapid asse...


    pub_type: 杂志文章


    authors: Melki R,Carlier MF,Pantaloni D

    更新日期:1990-09-25 00:00:00