Abstract：
:Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterization shows that the recombinant protein is identical with the natural protein and is devoid of posttranslational glycosylation, sulfation, and phosphorylation. The two C-terminal amino acids are proteolytically removed from the mature recombinant MGSA, indicating a length of 71 instead of the predicted 73 amino acids. The recombinant MGSA is mitogenically active on the Hs294T melanoma cells. The purified MGSA competes with interleukin 8 for binding to neutrophil receptors and exhibits neutrophil chemotactic activity equivalent to that of interleukin 8.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Balentien E,Han JH,Thomas HG,Wen DZ,Samantha AK,Zachariae CO,Griffin PR,Brachmann R,Wong WL,Matsushima Kdoi
10.1021/bi00496a011subject
Has Abstract,Author List Incompletepub_date
1990-11-06 00:00:00pages
10225-33issue
44eissn
0006-2960issn
1520-4995journal_volume
29pub_type
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