Two unique phosphorylation-driven signaling pathways crosstalk in Staphylococcus aureus to modulate the cell-wall charge: Stk1/Stp1 meets GraSR.

abstract：:Murine double-minute clone 2 protein (MDM2) is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumor suppressor protein. As part of the mechanism of p53 induction in response to DNA damage, a cluster of serine residues within the central acidic domain of MDM2 become hyp...

journal_title：Biochemistry

authors： Winter M,Milne D,Dias S,Kulikov R,Knippschild U,Blattner C,Meek D

更新日期：2004-12-28 00:00:00

abstract：:The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...

journal_title：Biochemistry

authors： Bolen DW,Yang M

更新日期：2000-12-12 00:00:00

abstract：:The bacterium Caulobacter crescentus is known to attach irreversibly to underwater surfaces by utilizing an adhesive structure called the holdfast, which exhibits the greatest known adhesive strength of any organism. The very small size of the holdfast (∼400 nm wide and ∼40 nm high) has made direct chemical analysis d...

journal_title：Biochemistry

authors： Nyarko A,Singla S,Barton HA,Dhinojwala A

更新日期：2020-09-22 00:00:00

abstract：:Seven mutant LH1 polypeptides of Rhodobactor sphaeroides have been isolated, and their behaviors in in vitro reconstitution of LH1 and its subunit complex have been characterized. Two mutants were selected to address the increased stability of the subunit complex of Rb. sphaeroides compared with that of Rhodobacter ca...

journal_title：Biochemistry

authors： Davis CM,Bustamante PL,Todd JB,Parkes-Loach PS,McGlynn P,Olsen JD,McMaster L,Hunter CN,Loach PA

更新日期：1997-03-25 00:00:00

abstract：:The binding of Sp1 transcription factor to DNA is considered a potential target for small ligands designed to interfere with gene transcription. We attempted to distinguish the direct inhibition of the Sp1-binding to DNA in vivo (cell culture) from more indirect effects due to the network of pathways that modulate cel...

journal_title：Biochemistry

authors： Mansilla S,Priebe W,Portugal J

更新日期：2004-06-15 00:00:00

abstract：:Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic...

journal_title：Biochemistry

authors： Fant K,Nordén B,Lincoln P

更新日期：2011-02-22 00:00:00

abstract：:The full-length cDNA encoding a novel human intracellular serine proteinase inhibitor has been sequenced and found to encode a 376 amino acid protein (M(r) approximately 42.5K) that we designate as cytoplasmic antiproteinase. Analysis of the primary structure revealed that the cytoplasmic antiproteinase has the majori...

journal_title：Biochemistry

authors： Morgenstern KA,Sprecher C,Holth L,Foster D,Grant FJ,Ching A,Kisiel W

更新日期：1994-03-22 00:00:00

abstract：:The formation of tyrosine from phenylalanine catalyzed by rat liver phenylalanine hydroxylase is coupled to the generation of a 4a-hydroxy adduct from the requisite tetrahydropterin cofactor. As indicated by its circular dichroism (CD) spectrum, the optical activity of the adduct generated from racemic 6-methyltetrahy...

journal_title：Biochemistry

authors： Dix TA,Bollag GE,Domanico PL,Benkovic SJ

更新日期：1985-06-04 00:00:00

abstract：:During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast AD...

journal_title：Biochemistry

authors： Plapp BV,Savarimuthu BR,Ferraro DJ,Rubach JK,Brown EN,Ramaswamy S

更新日期：2017-07-18 00:00:00

abstract：:The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences ...

journal_title：Biochemistry

authors： Sczepanski JT,Jacobs AC,Van Houten B,Greenberg MM

更新日期：2009-08-18 00:00:00

abstract：:Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...

journal_title：Biochemistry

authors： Longo A,Guanga GP,Rose RB

更新日期：2007-03-20 00:00:00

abstract：:Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...

journal_title：Biochemistry

authors： Arnold WR,Baylon JL,Tajkhorshid E,Das A

更新日期：2017-12-26 00:00:00

abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

journal_title：Biochemistry

authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

更新日期：2004-02-03 00:00:00

abstract：:Tandem calponin-homology (CH) domains are the most common actin-binding domains in proteins. However, structural principles underlying their function are poorly understood. These tandem domains exist in multiple conformations with varying degrees of inter-CH-domain interactions. Dystrophin and utrophin tandem CH domai...

journal_title：Biochemistry

authors： Bandi S,Singh SM,Mallela KM

更新日期：2015-09-08 00:00:00

abstract：:Membrane proteins are embedded in a lipid bilayer and maintain strong interactions with lipid molecules. Tightly bound lipids are responsible for vertical positioning and integration of proteins in the membrane and for assembly of multisubunit complexes and occasionally act as substrates. In this work we present the l...

journal_title：Biochemistry

authors： Wöhri AB,Wahlgren WY,Malmerberg E,Johansson LC,Neutze R,Katona G

更新日期：2009-10-20 00:00:00

abstract：:The mechanism of N-methyltryptophan oxidase, a flavin-dependent amine oxidase from Escherichia coli, was studied using a combination of kinetic isotope effects and theoretical calculations. The 15(kcat/Km) kinetic isotope effect for sarcosine oxidation is pH-dependent with a limiting value of 0.994-0.995 at high pH. D...

journal_title：Biochemistry

authors： Ralph EC,Hirschi JS,Anderson MA,Cleland WW,Singleton DA,Fitzpatrick PF

更新日期：2007-06-26 00:00:00

abstract：:A faithful transcription system for ribosomal RNA genes has been developed by using components from the small free-living amoeba Acanthamoeba castellanii. The system utilizes protein-free recombinant DNA as a template and in addition requires a crude cell-free extract containing RNA polymerase I and a transcription in...

journal_title：Biochemistry

authors： Paule MR,Iida CT,Perna PJ,Harris GH,Brown Shimer SL,Kownin P

更新日期：1984-08-28 00:00:00

abstract：:Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...

journal_title：Biochemistry

authors： Mancusso R,Cruz E,Cataldi M,Mendoza C,Fuchs J,Wang H,Yang X,Tasayco ML

更新日期：2004-04-06 00:00:00

abstract：:125I-Labeled enterotoxin from Clostridium perfringens was utilized to characterize the association of the enterotoxin with cells isolated from rabbit intestine and tissue homogenates from liver, kidney, and brain. The enterotoxin was found to bind in a specific and saturable manner to cells from intestine and to tissu...

journal_title：Biochemistry

authors： McDonel JL

更新日期：1980-10-14 00:00:00

abstract：:The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates t...

journal_title：Biochemistry

authors： Christenson JK,Richman JE,Jensen MR,Neufeld JY,Wilmot CM,Wackett LP

更新日期：2017-01-17 00:00:00

abstract：:Properties of the mycobacterial polymethylpolysaccharide-lipid complex have been investigated by fluorometric techniques. From the dissociation constant for the O-methyglucose polysaccharide-parinaric acid complex at 293 K, a Gibbs free energy (delta G degree) of -33.65 kJ/mol was obtained. The Kd decreased with incre...

journal_title：Biochemistry

authors： Kiho T,Ballou CE

更新日期：1988-07-26 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:4-Azido-2-nitrophenyl phosphate (ANPP) was synthesized and characterized. ANPP, unlabeled or labeled by 32P, was used as a photoreactive analogue of Pi to study the Pi binding site(s) in isolated F1-ATPase and inside-out particles from beef heart mitochondria. In the dark, the phosphate bond of ANPP was cleaved by alk...

journal_title：Biochemistry

authors： Lauquin G,Pougeois R,Vignais PV

更新日期：1980-09-30 00:00:00

abstract：:The homeodomain proteins a1 and alpha 2 act cooperatively to regulate cell type specific genes in yeast. The basis of the cooperativity is a weak interaction between the two proteins which forms heterodimers that bind DNA tightly and specifically. In this paper, we examine the mechanism of heterodimerization. We show ...

journal_title：Biochemistry

authors： Phillips CL,Stark MR,Johnson AD,Dahlquist FW

更新日期：1994-08-09 00:00:00

abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...

journal_title：Biochemistry

authors： Jung JH,Pendergast AM,Zipfel PA,Traugh JA

更新日期：2008-01-22 00:00:00

abstract：:S-Adenosylmethionine decarboxylase, a pyruvoyl-containing decarboxylase, is inactivated in a time-dependent process under turnover conditions. The inactivation is dependent on the presence of both substrate and Mg2+, which is also required for enzyme activity. The rate of inactivation is dependent on the concentration...

journal_title：Biochemistry

authors： Anton DL,Kutny R

更新日期：1987-10-06 00:00:00

abstract：:Potent inhibitors of human cysteine proteases of the papain family have been made and assayed versus a number of relevant family members. We describe the synthesis of peptide alpha-ketoheterocyclic inhibitors that occupy binding subsites S1'-S3 of the cysteine protease substrate recognition cleft and that form a rever...

journal_title：Biochemistry

authors： McGrath ME,Sprengeler PA,Hill CM,Martichonok V,Cheung H,Somoza JR,Palmer JT,Janc JW

更新日期：2003-12-30 00:00:00

abstract：:The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...

journal_title：Biochemistry

authors： Mercier P,Li MX,Sykes BD

更新日期：2000-03-21 00:00:00

abstract：:The binding of various forms of vanadate to myosin and myosin subfragment 1 (S-1) was studied by 51V NMR at increasing vanadate concentrations between 0.06 and 1.0 mM. The distribution of the various forms of vanadate in the solution depended on the total concentration of vanadate. At low concentrations, the predomina...

journal_title：Biochemistry

authors： Ringel I,Peyser YM,Muhlrad A

更新日期：1990-09-25 00:00:00

abstract：:Control of protein phosphatases is now understood to depend on binding to a variety of regulatory or targeting subunits to form holoenzymes with restricted localization and substrate specificity. In addition, the catalytic subunits of both type-1 and type-2 phosphatases bind specific inhibitor proteins. Here, we repor...

journal_title：Biochemistry

authors： Eto M,Karginov A,Brautigan DL

更新日期：1999-12-21 00:00:00