Structural basis for induced fit mechanisms in DNA recognition by the Pdx1 homeodomain.

abstract：:The targeting of the insulin-responsive glucose transporter, GLUT-4, to an intracellular compartment in adipocytes and muscle is one of the key features responsible for the unique insulin sensitivity of this transporter. Through expression of epitope-tagged GLUT-4 mutants in 3T3-L1 adipocytes, two motifs have been ide...

journal_title：Biochemistry

authors： Melvin DR,Marsh BJ,Walmsley AR,James DE,Gould GW

更新日期：1999-02-02 00:00:00

abstract：:Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...

journal_title：Biochemistry

authors： Jumblatt JE,Schlup V,Burger MM

更新日期：1980-03-04 00:00:00

abstract：:The nuclear tRNA 3' processing activity from wheat has been characterized and partially purified. Several characteristics of the wheat nuclear 3' processing enzyme now allow this activity to be distinguished from its mitochondrial counterpart. The nuclear enzyme is an endonuclease, which we termed nuclear RNase Z. The...

journal_title：Biochemistry

authors： Mayer M,Schiffer S,Marchfelder A

更新日期：2000-02-29 00:00:00

abstract：:Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is a monotopic membrane protein anchored to the membrane by an N-terminal in-plane amphipathic alpha-helix. This membrane anchor is essential for the assembly of a functional viral replication complex. Although amino acid sequences differ considerably, putative m...

journal_title：Biochemistry

authors： Sapay N,Montserret R,Chipot C,Brass V,Moradpour D,Deléage G,Penin F

更新日期：2006-02-21 00:00:00

abstract：:Ala-based peptides form marginally stable helices at low temperature and are conventionally considered as mixtures of alpha-helix and random coil. However, recent work with doubly spin-labeled peptides suggests that short 16-residue sequences contain a significant fraction of 3(10)-helix near the N-terminus (positions...

journal_title：Biochemistry

authors： Fiori WR,Miick SM,Millhauser GL

更新日期：1993-11-16 00:00:00

abstract：:The red cell membrane is stabilized by a spectrin/actin-based cortical cytoskeleton connected to the phospholipid bilayer via multiple protein bridges. By virtue of its interaction with ankyrin and adducin, the anion transporter, band 3 (AE1), contributes prominently to these bridges. In a previous study, we demonstra...

journal_title：Biochemistry

authors： Grey JL,Kodippili GC,Simon K,Low PS

更新日期：2012-08-28 00:00:00

abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

journal_title：Biochemistry

authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

更新日期：2000-05-30 00:00:00

abstract：:There are a small number of peptides derived from proteins that have a propensity to adopt structure in aqueous solution which is similar to the structure they possess in the parent protein. There are far fewer examples of protein fragments which adopt stable nonnative structures in isolation. Understanding how nonnat...

journal_title：Biochemistry

authors： Demarest SJ,Hua Y,Raleigh DP

更新日期：1999-06-01 00:00:00

abstract：:The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...

journal_title：Biochemistry

authors： Mercurio FA,Marasco D,Pirone L,Pedone EM,Pellecchia M,Leone M

更新日期：2012-03-13 00:00:00

abstract：:Activated receptors with a tyrosine kinase activity induce a variety of responses like changes in the differentiation and mitogenic status of cells. These responses are mediated in part by p21ras. Some of these activated receptors induce in certain cell types a pronounced, but transient, increase in Ras-GTP. We have s...

journal_title：Biochemistry

authors： Osterop AP,Medema RH,Ouwens DM,Van der Zon GC,Möller W,Maassen JA

更新日期：1994-06-14 00:00:00

abstract：:The concentration of hyaluronic acid, chondroitin sulfate, and heparan sulfate was measured in rat brain at 2-day intervals from birth to 1 month of age, and in 40-day-old and adult animals. The levels of all three glycosaminoglycans increased after birth to reach a peak at 7 days after which they declined steadily, a...

journal_title：Biochemistry

authors： Margolis RU,Margolis RK,Chang LB,Preti C

更新日期：1975-01-14 00:00:00

abstract：:Eukaryotic initiation factor 5 (eIF5) plays multiple roles in translation initiation. Its N-terminal domain functions as a GTPase-activator protein (GAP) for GTP bound to eIF2, while its C-terminal region nucleates the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydroly...

journal_title：Biochemistry

authors： Conte MR,Kelly G,Babon J,Sanfelice D,Youell J,Smerdon SJ,Proud CG

更新日期：2006-04-11 00:00:00

abstract：:We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the ...

journal_title：Biochemistry

authors： Breyton C,Nandha B,Johnson GN,Joliot P,Finazzi G

更新日期：2006-11-14 00:00:00

abstract：:Soluble guanylate cyclase (sGC) is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. sGC is activated by nitric oxide (NO) and therefore plays a central role in NO signal transduction. Activation of sGC by NO is believed to be mediated by the interaction between NO and the heme of sGC. Spectroscopic a...

journal_title：Biochemistry

authors： Zhao Y,Marletta MA

更新日期：1997-12-16 00:00:00

abstract：:We have used a site-specific approach to investigate the mutagenic potential of (+)- and (-)-trans-anti-benzo[a]pyrene diol epoxide (BPDE) DNA adducts. Oligodeoxyribonucleotides (5'TCCTCCTG1G2-CCTCTC), modified at the exocyclic amino groups of G1 or G2, were incorporated into a single-stranded shuttle vector and intro...

journal_title：Biochemistry

authors： Moriya M,Spiegel S,Fernandes A,Amin S,Liu T,Geacintov N,Grollman AP

更新日期：1996-12-24 00:00:00

abstract：:The flexibility and curvature of duplex DNAs containing mismatched sites have been monitored as a function of temperature. The diffusion coefficients are dependent on the flexibility and the curvature of the DNA, and these have been determined by NMR-based methods. The diffusion coefficients, D, depend on a Boltzmann ...

journal_title：Biochemistry

authors： Marathias VM,Jerkovic B,Arthanari H,Bolton PH

更新日期：2000-01-11 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...

journal_title：Biochemistry

authors： Chatron N,Abi Khalil R,Benoit E,Lattard V

更新日期：2020-04-07 00:00:00

abstract：:Caldesmon from chicken gizzard muscle has been examined for its ability to interact with caltropin using affinity chromatography and the fluorescent probe acrylodan. The action of caltropin on the inhibitory effect of caldesmon on actomyosin ATPase was also studied. Like calmodulin, caltropin could release the inhibit...

journal_title：Biochemistry

authors： Mani RS,McCubbin WD,Kay CM

更新日期：1992-12-01 00:00:00

abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...

journal_title：Biochemistry

authors： Orban T,Huang CC,Homan KT,Jastrzebska B,Tesmer JJ,Palczewski K

更新日期：2012-04-24 00:00:00

abstract：:To study the function of exon 21 of the insulin receptor, a mutant human insulin receptor lacking this domain was constructed. The mutant HIR delta E21 cDNA was transfected into Rat-1 fibroblasts and stable cell lines were selected. The HIR delta E21 receptors were expressed on the cell surface, and they bound insulin...

journal_title：Biochemistry

authors： Rolband GC,Williams JF,Webster NJ,Hsu D,Olefsky JM

更新日期：1993-12-14 00:00:00

abstract：:A high-resolution solution conformation of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA, GCCGSYPNAACHPCSCKDROSYCGQ-NH2, has been determined by two-dimensional 1H NMR methods and distance geometry calculations. The total of 324 NOE-derived interproton distance restraints including 33 long-range NOE restraints a...

journal_title：Biochemistry

authors： Han KH,Hwang KJ,Kim SM,Kim SK,Gray WR,Olivera BM,Rivier J,Shon KJ

更新日期：1997-02-18 00:00:00

abstract：:RXPA380 (Cbz-PhePsi[PO(2)CH]Pro-Trp-OH) was reported recently as the first highly selective inhibitor of the C-domain of somatic angiotensin-converting enzyme (ACE), able to differentiate the two active sites of somatic ACE by a selectivity factor of more than 3 orders of magnitude. The contribution of each RXPA380 re...

journal_title：Biochemistry

authors： Georgiadis D,Cuniasse P,Cotton J,Yiotakis A,Dive V

更新日期：2004-06-29 00:00:00

abstract：:Vitamin B12-dependent methionine synthase catalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine via the enzyme-bound cofactor methylcobalamin. To carry out this reaction, the enzyme must alternately stabilize six-coordinate methylcobalamin and four-coordinate cob(I)alamin oxidation state...

journal_title：Biochemistry

authors： Jarrett JT,Amaratunga M,Drennan CL,Scholten JD,Sands RH,Ludwig ML,Matthews RG

更新日期：1996-02-20 00:00:00

abstract：:Most proteins are denatured by heat treatment, and the process is usually irreversible. However, some proteins, such as hyperthermophilic proteins are known to be stable even at the boiling temperature of water. We here describe a systematic investigation of thermal behavior of proteins by purifying and characterizing...

journal_title：Biochemistry

authors： Kim TD,Ryu HJ,Cho HI,Yang CH,Kim J

更新日期：2000-12-05 00:00:00

abstract：:The linker for activation of T-cells (LAT) is a palmitoylated integral membrane adaptor protein that resides in lipid membrane rafts and contains nine consensus putative tyrosine phosphorylation sites, several of which have been shown to serve as SH2 binding sites. Upon T-cell antigen receptor (TCR/CD3) engagement, LA...

journal_title：Biochemistry

authors： Perez-Villar JJ,Whitney GS,Sitnick MT,Dunn RJ,Venkatesan S,O'Day K,Schieven GL,Lin TA,Kanner SB

更新日期：2002-08-27 00:00:00

abstract：:It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y

更新日期：1992-01-28 00:00:00

abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

journal_title：Biochemistry

authors： Kretzschmar KM,Mendelson RA,Morales MF

更新日期：1978-06-13 00:00:00

abstract：:Exchangeable imidazole NH proton nuclear magnetic resonance (NMR) signals of the proximal histidine F8, which is directly coordinated to the paramagnetic porphyrin cobalt ion, have been measured for cobalt-substituted deoxyhemoglobins (deoxy-CoHb) and iron-cobalt hybrid hemoglobins, alpha (Co)2 beta (Fe)2 and alpha (F...

journal_title：Biochemistry

authors： Inubushi T,Ikeda-Saito M,Yonetani T

更新日期：1983-06-07 00:00:00

abstract：:The technique of fluorescence recovery after photobleaching is used to perform an extensive study of the lateral diffusion of a phospholipid probe in the binary mixture dimyristoylphosphatidylcholine/cholesterol, above the melting temperature of the phospholipid. In the regions of the phase diagram where a single liqu...

journal_title：Biochemistry

authors： Almeida PF,Vaz WL,Thompson TE

更新日期：1992-07-28 00:00:00