Cell-cell recognition: specific binding of Microciona sponge aggregation factor to homotypic cells and the role of calcium ions.

abstract：:Methylated DNA binding protein 2 (MeCP2) is a methyl CpG binding protein whose key role is the recognition of epigenetic information encoded in DNA methylation patterns. Mutation or misregulation of MeCP2 function leads to Rett syndrome as well as a variety of other autism spectrum disorders. Here, we have analyzed in...

journal_title：Biochemistry

authors： Ghosh RP,Nikitina T,Horowitz-Scherer RA,Gierasch LM,Uversky VN,Hite K,Hansen JC,Woodcock CL

更新日期：2010-05-25 00:00:00

abstract：:Toc34 is a transmembrane protein located in the outer envelope membrane of chloroplasts and involved in transit peptide recognition. The cytosolic region of Toc34 reveals 34% alpha-helical and 26% beta-strand structure and is stabilized by intramolecular electrostatic interaction. Toc34 binds both chloroplast preprote...

journal_title：Biochemistry

authors： Schleiff E,Soll J,Sveshnikova N,Tien R,Wright S,Dabney-Smith C,Subramanian C,Bruce BD

更新日期：2002-02-12 00:00:00

abstract：:Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...

journal_title：Biochemistry

authors： Tsai CH,Shen TJ,Ho NT,Ho C

更新日期：1999-07-06 00:00:00

abstract：:To understand relationships between membrane-binding properties of cytolytic peptides and resulting cytotoxicity, we investigated interactions of dermaseptin analogues with model bilayers by means of surface plasmon resonance. First, we tested the system by comparing two native dermaseptins, S1 and S4, whose binding p...

journal_title：Biochemistry

authors： Gaidukov L,Fish A,Mor A

更新日期：2003-11-11 00:00:00

abstract：:IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...

journal_title：Biochemistry

authors： Pelton JG,Torchia DA,Meadow ND,Wong CY,Roseman S

更新日期：1991-10-15 00:00:00

abstract：:To better elucidate RNA structure-function relationships and to improve the design of pharmaceutical agents that target specific RNA motifs, an understanding of RNA primary, secondary, and tertiary structure is necessary. The prediction of RNA secondary structure from sequence is an intermediate step in predicting RNA...

journal_title：Biochemistry

authors： Hausmann NZ,Znosko BM

更新日期：2012-07-03 00:00:00

abstract：:Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed for thiol content and thiol reactivity. It was found that peak 1 and peak 2 contained two and three thiol groups, respectively, per mole of enzyme. This result...

journal_title：Biochemistry

authors： Kóródi I,Asbóth B,Polgár L

更新日期：1986-11-04 00:00:00

abstract：:Optical detection of triplet-state magnetic resonance (ODMR) is employed to study the complexes formed between gene 32 protein (GP32), a single-stranded DNA-binding protein from bacteriophage T4, and the heavy-atom-derivatized polynucleotides poly(5-HgU) and poly(5-BrU). The triplet-state properties of some of the try...

journal_title：Biochemistry

authors： Khamis MI,Maki AH

更新日期：1986-10-07 00:00:00

abstract：:Artificial genetic systems have been developed by synthetic biologists over the past two decades to include additional nucleotides that form additional nucleobase pairs independent of the standard T:A and C:G pairs. Their use in various tools to detect and analyze DNA and RNA requires polymerases that synthesize duple...

journal_title：Biochemistry

authors： Laos R,Shaw R,Leal NA,Gaucher E,Benner S

更新日期：2013-08-06 00:00:00

abstract：:The three-dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization. The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from bioche...

journal_title：Biochemistry

authors： Borer PN,Lin Y,Wang S,Roggenbuck MW,Gott JM,Uhlenbeck OC,Pelczer I

更新日期：1995-05-16 00:00:00

abstract：:Equilibrium binding experiments are widely used for the accurate characterization of binding and competitive binding behavior in biological systems. Modern high-throughput discovery efforts in chemical biology rely heavily upon this principle. Here, we derive exact analytical expressions for general competitive bindin...

journal_title：Biochemistry

authors： Roehrl MH,Wang JY,Wagner G

更新日期：2004-12-28 00:00:00

abstract：:The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

journal_title：Biochemistry

authors： Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

更新日期：1990-06-19 00:00:00

abstract：:The pathological prion protein PrP(Sc) is the only known component of the infectious prion. In cells infected with prions, PrP(Sc) is formed posttranslationally by the refolding of the benign cell surface glycoprotein PrP(C) into an aberrant conformation. The two PrP isoforms possess very different properties, as PrP(...

journal_title：Biochemistry

authors： Tzaban S,Friedlander G,Schonberger O,Horonchik L,Yedidia Y,Shaked G,Gabizon R,Taraboulos A

更新日期：2002-10-22 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...

journal_title：Biochemistry

authors： Kirpich JS,Chang CW,Franse J,Yu Q,Escobar FV,Jenkins AJ,Martin SS,Narikawa R,Ames JB,Lagarias JC,Larsen DS

更新日期：2021-01-13 00:00:00

abstract：:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximate...

journal_title：Biochemistry

authors： Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

更新日期：2004-08-24 00:00:00

abstract：:Nonsense mutations that result in premature stop codons in the HBB gene cause β-thalassemia. This disease is characterized by a reduced hemoglobin level due to the lack of β-globin. Compounds that induce translational readthrough across the thalassemia-causing premature stop codon will have therapeutic benefits. Curre...

journal_title：Biochemistry

authors： Kar D,Sellamuthu K,Kumar SD,Eswarappa SM

更新日期：2020-01-14 00:00:00

abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

journal_title：Biochemistry

authors： Sun M,Leyh TS

更新日期：2006-09-26 00:00:00

abstract：:Conformational and environmental changes in the functionally significant amino-terminal region of human parathyroid hormone (hPTH), induced by solvent or by complexation with acidic lipid, have been investigated. Structural perturbations were monitored by their effect on the fluorescence decay kinetics of the single t...

journal_title：Biochemistry

authors： Willis KJ,Szabo AG

更新日期：1992-09-22 00:00:00

abstract：:Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...

journal_title：Biochemistry

authors： Takigami T,Takeuchi F,Nakagawa M,Hase T,Tsubaki M

更新日期：2003-07-15 00:00:00

abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

journal_title：Biochemistry

authors： Abdelhamid MAS,Gates AJ,Waller ZAE

更新日期：2019-01-29 00:00:00

abstract：:The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...

journal_title：Biochemistry

authors： Ma QF,Bathurst IC,Barr PJ,Kenyon GL

更新日期：1992-02-11 00:00:00

abstract：:A full-length cDNA for dopamine beta-monooxygenase (D beta M) from bovine adrenal glands has been cloned and sequenced. The soluble and membrane-derived forms of D beta M have also been sequenced from their N-termini. While the observed sequences for the soluble protein correspond to those previously reported [Joh, T....

journal_title：Biochemistry

authors： Taljanidisz J,Stewart L,Smith AJ,Klinman JP

更新日期：1989-12-26 00:00:00

abstract：:Biochemical characterization of the inhibition mechanism of Deltalac-acetogenins synthesized in our laboratory indicated that they are a new type of inhibitor of bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I) [Murai, M., et al. (2006) Biochemistry 45, 9778-9787]. To identify the binding site of ...

journal_title：Biochemistry

authors： Kakutani N,Murai M,Sakiyama N,Miyoshi H

更新日期：2010-06-15 00:00:00

abstract：:Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. X-ray crystal structures of arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and urea, complete a set of struct...

journal_title：Biochemistry

authors： Cox JD,Cama E,Colleluori DM,Pethe S,Boucher JL,Mansuy D,Ash DE,Christianson DW

更新日期：2001-03-06 00:00:00

abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

journal_title：Biochemistry

authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

更新日期：1995-12-05 00:00:00

abstract：:In the absence of ligands, dihydrofolate reductase from Escherichia coli exists in at least two interconvertible conformations, only one of which binds NADPH with high affinity. This equilibrium is pH dependent, involving an ionizable group of the enzyme (pK approximately 5.5), and the proportion of the NADPH-binding ...

journal_title：Biochemistry

authors： Dunn SM,Lanigan TM,Howell EE

更新日期：1990-09-18 00:00:00

abstract：:Diethyl pyrocarbonate inhibited diaphorase activity of ferredoxin-NADP+ oxidoreductase with a second-order rate constant of 2 mM-1 X min-1 at pH 7.0 and 20 degrees C, showing a concomitant increase in absorbance at 242 nm due to formation of carbethoxyhistidyl derivatives. Activity could be restored by hydroxylamine, ...

journal_title：Biochemistry

authors： Carrillo N,Vallejos RH

更新日期：1983-12-06 00:00:00

abstract：:The requirement for Ca2+ in the Mn(2+)-dependent photoactivation of oxygen evolution was re-evaluated using 17 kDa/24 kDa-less photosystem II (PSII) membranes depleted of (Mn)4-clusters by NH2OH extraction. At optimum conditions (1 mM Mn2+/10 microM 2,6-dichlorphenolindophenol (DCIP)/20 mM Ca2+), the light-induced inc...

journal_title：Biochemistry

authors： Chen C,Kazimir J,Cheniae GM

更新日期：1995-10-17 00:00:00

abstract：:The gamma-aminobutyric acid transporter of rat brain synaptic vesicles was reconstituted in proteoliposomes, and its activity was studied in response to artificially created membrane potentials or proton gradients. Changes of the membrane potential were monitored using the dyes oxonol VI and 3,3'-diisopropylthiodicarb...

journal_title：Biochemistry

authors： Hell JW,Edelmann L,Hartinger J,Jahn R

更新日期：1991-12-24 00:00:00

abstract：:The effect of long-term storage on the electronic absorption spectrum and the kinetic properties of cytochrome c peroxidase has been investigated. No detectable differences were observed between freshly isolated enzyme and enzyme stored below -20 degrees C, in the crystalline state, for up to 41 months. The electronic...

journal_title：Biochemistry

authors： Vitello LB,Huang M,Erman JE

更新日期：1990-05-08 00:00:00