当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > CYP51 from Trypanosoma brucei is obtusifoliol-specific.

CYP51 from Trypanosoma brucei is obtusifoliol-specific.

Abstract：

:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximately 25% identical to sterol 14alpha-demethylases from other biological kingdoms. Differences of residues conserved throughout the rest of the CYP51 family that align with the BC-loop and helices F and G of CYP51 from Mycobacterium tuberculosis (MT)) imply possible alterations in the topology of the active site cavity of the protozoan enzymes. CYP51 and cytochrome P450 reductase (CPR) from TB were cloned, expressed in Escherichia coli, and purified. The P450 has normal spectral features (including absolute absorbance, carbon monoxide, and ligand binding spectra), is efficiently reduced by TB and rat CPR but demonstrates altered specificity in comparison with human CYP51 toward three tested azole inhibitors, and contrary to the human, Candida albicans, and MT isoforms, reveals profound substrate preference toward obtusifoliol (turnover 5.6 min(-1)). It weakly interacts with the other known CYP51 substrates; slow lanosterol conversion predominantly produces the 14alpha-carboxyaldehyde intermediate. Although obtusifoliol specificity is typical for plant isoforms of CYP51, the set of sterol biosynthetic enzymes in the protozoan genomes together with available information about sterol composition of kinetoplastid cells suggest that the substrate preference of TBCYP51 may reflect a novel sterol biosynthetic pathway in Trypanosomatidae.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

doi

10.1021/bi048967t

subject

Has Abstract

pub_date

2004-08-24 00:00:00

pages

10789-99

issue

33

eissn

0006-2960

issn

1520-4995

journal_volume

43

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Solute diffusion in lipid bilayer membranes: an atomic level study by molecular dynamics simulation.

    abstract：:To elucidate the mechanism of solute diffusion through lipid bilayer membranes, nearly 4 ns of molecular dynamics simulations of solutes in phospholipid bilayers was conducted. The study, the first atomic level study of solute diffusion in a lipid bilayer, involved four simulations of an all-atom representation of a f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00210a010

    authors： Bassolino-Klimas D,Alper HE,Stouch TR

    更新日期：1993-11-30 00:00:00

  • Structure and inhibition of a quorum sensing target from Streptococcus pneumoniae.

    abstract：:Streptococcus pneumoniae 5'-methylthioadenosine/S-adenosylhomocysteine hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-methylthioribose or S-ribosylhomocysteine (SRH). MTAN is not found in mammals but is involved in bacterial quorum sensing. MTAN gene disruption affects ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061184i

    authors： Singh V,Shi W,Almo SC,Evans GB,Furneaux RH,Tyler PC,Painter GF,Lenz DH,Mee S,Zheng R,Schramm VL

    更新日期：2006-10-31 00:00:00

  • Oxidation-reduction properties of compounds I and II of Arthromyces ramosus peroxidase.

    abstract：:At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00184a038

    authors： Farhangrazi ZS,Copeland BR,Nakayama T,Amachi T,Yamazaki I,Powers LS

    更新日期：1994-05-10 00:00:00

  • Helix stop signals in proteins and peptides: the capping box.

    abstract：:The alpha-helix [Pauling, L., Corey, R. B., & Branson, H. R. (1951) Proc. Natl. Acad. Sci. U.S.A. 37, 205-211] is a common motif in both proteins and peptides. Despite intense investigation, predictive understanding of helices is still lacking. A recent hypothesis [Presta, L. G., & Rose, G. D. (1988) Science 240, 1632...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00081a001

    authors： Harper ET,Rose GD

    更新日期：1993-08-03 00:00:00

  • Cross-talk between protein kinase C-alpha (PKC-alpha) and -delta (PKC-delta): PKC-alpha elevates the PKC-delta protein level, altering its mRNA transcription and degradation.

    abstract：:Studies utilizing the overexpression of individual isoforms indicated that both PKC-alpha and -delta promote a number of biological effects, including inhibition of DNA synthesis associated with rearrangements of the actin cytoskeleton in the murine B-cell lymphoma (Baf3), differentiation of the murine promyelocyte li...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9731807

    authors： Romanova LY,Alexandrov IA,Nordan RP,Blagosklonny MV,Mushinski JF

    更新日期：1998-04-21 00:00:00

  • General peroxidase activity of G-quadruplex-hemin complexes and its application in ligand screening.

    abstract：:DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9006786

    authors： Cheng X,Liu X,Bing T,Cao Z,Shangguan D

    更新日期：2009-08-25 00:00:00

  • Exonuclease-polymerase active site partitioning of primer-template DNA strands and equilibrium Mg2+ binding properties of bacteriophage T4 DNA polymerase.

    abstract：:The binding of bacteriophage T4 DNA polymerase (T4 pol) to primer-template DNA with 2-aminopurine (2AP) located at the primer terminus results in the formation of a hyperfluorescent 2AP state. Changes in this hyperfluorescent state were utilized to investigate the fractional concentration of primer-templates bound at ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980074b

    authors： Beechem JM,Otto MR,Bloom LB,Eritja R,Reha-Krantz LJ,Goodman MF

    更新日期：1998-07-14 00:00:00

  • Activator-dependent preinduction binding of sigma-70 RNA polymerase at the metal-regulated mer promoter.

    abstract：:Expression of the Tn21 mercury-resistance (mer) locus is controlled by the merR gene product, which represses mer structural gene (merTPCAD) transcription in the absence of mercuric ion [Hg(II)] and activates it in the presence of Hg(II). In vivo DNA methylation of the mer regulatory region (merOP) shows that, with or...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00493a011

    authors： Heltzel A,Lee IW,Totis PA,Summers AO

    更新日期：1990-10-16 00:00:00

  • RNA-ligand interactions: affinity and specificity of aminoglycoside dimers and acridine conjugates to the HIV-1 Rev response element.

    abstract：:Semisynthetic aminoglycoside derivatives may provide a means to selectively target viral RNA sites, including the HIV-1 Rev response element (RRE). The design, synthesis, and evaluation of derivatives based upon neomycin B, kanamycin A, and tobramycin conjugates of 9-aminoacridine are presented. To evaluate the import...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034766y

    authors： Luedtke NW,Liu Q,Tor Y

    更新日期：2003-10-07 00:00:00

  • Investigation of the origin of the "S3" EPR signal from the oxygen-evolving complex of photosystem 2: the role of tyrosine Z.

    abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00134a005

    authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

    更新日期：1992-05-19 00:00:00

  • Studies on the replication of the ring opened formamidopyrimidine, Fapy.dG in Escherichia coli.

    abstract：:Fapy.dG is produced in DNA as a result of oxidative stress from a precursor that also forms OxodG. Bypass of Fapy.dG in a shuttle vector in COS-7 cells produces G --> T transversions slightly more frequently than does OxodG (Kalam, M. A., et al. (2006) Nucleic Acids Res. 34, 2305). The effect of Fapy.dG on replication...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700628c

    authors： Patro JN,Wiederholt CJ,Jiang YL,Delaney JC,Essigmann JM,Greenberg MM

    更新日期：2007-09-04 00:00:00

  • Transition state in DNA polymerase β catalysis: rate-limiting chemistry altered by base-pair configuration.

    abstract：:Kinetics studies of dNTP analogues having pyrophosphate-mimicking β,γ-pCXYp leaving groups with variable X and Y substitution reveal striking differences in the chemical transition-state energy for DNA polymerase β that depend on all aspects of base-pairing configurations, including whether the incoming dNTP is a puri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500101z

    authors： Oertell K,Chamberlain BT,Wu Y,Ferri E,Kashemirov BA,Beard WA,Wilson SH,McKenna CE,Goodman MF

    更新日期：2014-03-25 00:00:00

  • The linker between SH2 and kinase domains positively regulates catalysis of the Tec family kinases.

    abstract：:Tec family nonreceptor tyrosine kinases are key immunological enzymes that control processes that range from T and B cell development to reorganization of the actin cytoskeleton. The full-length Tec kinases have been resistant to crystallization. This lack of structural data and the paucity of in vitro biochemical dat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602512e

    authors： Joseph RE,Min L,Andreotti AH

    更新日期：2007-05-08 00:00:00

  • Looking at human cytosolic sialidase NEU2 structural features with an interdisciplinary approach.

    abstract：:Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations. The results show the prevalence of β-strands together with a considerable amount of α-helical structure, while in the solid state, from bot...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500249r

    authors： Monti E,Benaglia G,Mozzi A,Fusi P,Longhi G,Gangemi F,Castiglioni E,Woody RW,Fornili SL,Abbate S

    更新日期：2014-08-19 00:00:00

  • Engineering the independent folding of the subtilisin BPN' pro-domain: correlation of pro-domain stability with the rate of subtilisin folding.

    abstract：:The 77-amino acid pro-domain greatly accelerates the in vitro folding of subtilisin in a bimolecular reaction whose product is a tight complex between folded subtilisin and folded pro-domain. In this complex the pro-domain has a compact structure with a four-stranded antiparallel beta-sheet and two three-turn alpha-he...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972741r

    authors： Wang L,Ruan B,Ruvinov S,Bryan PN

    更新日期：1998-03-03 00:00:00

  • The hydroxyl of threonine 13 of the bovine 70-kDa heat shock cognate protein is essential for transducing the ATP-induced conformational change.

    abstract：:The mechanism by which ATP binding transduces a conformational change in 70-kDa heat shock proteins that results in release of bound peptides remains obscure. Wei and Hendershot demonstrated that mutating Thr37 of hamster BiP to glycine impeded the ATP-induced conformational change, as monitored by proteolysis [(1995)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981510x

    authors： Sousa MC,McKay DB

    更新日期：1998-11-03 00:00:00

  • Sequence-Dependent Promoter Escape Efficiency Is Strongly Influenced by Bias for the Pretranslocated State during Initial Transcription.

    abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00272

    authors： Skancke J,Bar N,Kuiper M,Hsu LM

    更新日期：2015-07-21 00:00:00

  • Urea Mimics Nucleobases by Preserving the Helical Integrity of B-DNA Duplexes via Hydrogen Bonding and Stacking Interactions.

    abstract：:Urea lesions are formed in DNA because of free radical damage of the thymine base, and their occurrence in DNA blocks DNA polymerases, which has deleterious consequences. Recently, it has been shown that urea is capable of forming hydrogen bonding and stacking interactions with nucleobases, which are responsible for t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00309

    authors： Suresh G,Padhi S,Patil I,Priyakumar UD

    更新日期：2016-10-11 00:00:00

  • Structural studies on the active site of Escherichia coli RNA polymerase. 2. Geometrical relationship of the interacting substrates.

    abstract：:Since a major function of RNA polymerase must be to bring together substrates in the optimal configuration for internucleotide bond formation, studies have been undertaken to understand the geometrical relationship of the two substrates. A model has been constructed for the geometry of interaction of two ATP molecules...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00477a017

    authors： Beal RB,Pillai RP,Chuknyisky PP,Levy A,Tarien E,Eichhorn GL

    更新日期：1990-06-26 00:00:00

  • Pinpoint chemical modification of Asp160 in the 49 kDa subunit of bovine mitochondrial complex I via a combination of ligand-directed tosyl chemistry and click chemistry.

    abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501342w

    authors： Masuya T,Murai M,Morisaka H,Miyoshi H

    更新日期：2014-12-16 00:00:00

  • Effects of Olive Metabolites on DNA Cleavage Mediated by Human Type II Topoisomerases.

    abstract：:Several naturally occurring dietary polyphenols with chemopreventive or anticancer properties are topoisomerase II poisons. To identify additional phytochemicals that enhance topoisomerase II-mediated DNA cleavage, a library of 341 Mediterranean plant extracts was screened for activity against human topoisomerase IIα....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00162

    authors： Vann KR,Sedgeman CA,Gopas J,Golan-Goldhirsh A,Osheroff N

    更新日期：2015-07-28 00:00:00

  • Electrostatic Repulsion during Ferritin Assembly and Its Screening by Ions.

    abstract：:Escherichia coli non-heme-binding ferritin A (EcFtnA) is a spherical cagelike protein that is composed of 24 identical subunits. EcFtnA dissociates into 2-mers under acidic conditions and can reassemble into the native structure when the pH is increased. To understand how electrostatic interactions influence the assem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01197

    authors： Sato D,Takebe S,Kurobe A,Ohtomo H,Fujiwara K,Ikeguchi M

    更新日期：2016-01-26 00:00:00

  • Side-chain interactions in the folding pathway of a Fyn SH3 domain mutant studied by relaxation dispersion NMR spectroscopy.

    abstract：:A major challenge to the study of protein folding is the fact that intermediate states along the reaction pathway are generally unstable and thus difficult to observe. Recently developed NMR relaxation dispersion experiments present an avenue to accessing such states, providing kinetic, thermodynamic, and structural i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051771o

    authors： Mittermaier A,Korzhnev DM,Kay LE

    更新日期：2005-11-29 00:00:00

  • Active site modifications in a double mutant of liver alcohol dehydrogenase: structural studies of two enzyme-ligand complexes.

    abstract：:The oxidation of alcohol to aldehyde by horse liver alcohol dehydrogenase (LADH) requires the transfer of a hydride ion from the alcohol substrate to the cofactor nicotinamide adenine dinucleotide (NAD). A quantum mechanical tunneling contribution to this hydride transfer step has been demonstrated in a number of LADH...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973184b

    authors： Colby TD,Bahnson BJ,Chin JK,Klinman JP,Goldstein BM

    更新日期：1998-06-30 00:00:00

  • Interaction of lipoprotein lipase with phospholipid vesicles: effect on protein and lipid structure.

    abstract：:The interaction of lipoprotein lipase (LpL) and a nonhydrolyzable phosphatidylcholine, 1,2-ditetradecyl-rac-glycero-3-phosphocholine (C14-ether-PC), has been studied by several physical methods. Analysis of the circular dichroic spectrum of LpL gave the following fractional conformation: 35% alpha-helix, 30% beta-plea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00352a020

    authors： McLean LR,Larsen WJ,Jackson RL

    更新日期：1986-02-25 00:00:00

  • 6-(Methoxymethylene)penicillanic acid: inactivator of RTEM beta-lactamase from Escherichia coli.

    abstract：:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00319a025

    authors： Brenner DG,Knowles JR

    更新日期：1984-11-20 00:00:00

  • Reversible transition between the surface trimer and membrane-inserted monomer of annexin 12.

    abstract：:Under mildly acidic conditions, annexin 12 (ANX) inserts into lipid membranes to form a transbilayer pore [Langen, R., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14060]. In this study, we have addressed the question of the oligomeric state of ANX in this transbilayer conformation by means of Forster-type resonanc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047805u

    authors： Ladokhin AS,Haigler HT

    更新日期：2005-03-08 00:00:00

  • Purification and spectroscopic characterization of photosystem II reaction center complexes isolated with or without Triton X-100.

    abstract：:The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the composition of pho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960991m

    authors： Eijckelhoff C,van Roon H,Groot ML,van Grondelle R,Dekker JP

    更新日期：1996-10-01 00:00:00

  • High-resolution structure of an HIV zinc fingerlike domain via a new NMR-based distance geometry approach.

    abstract：:A new method is described for determining molecular structures from NMR data. The approach utilizes 2D NOESY back-calculations to generate simulated spectra for structures obtained from distance geometry (DG) computations. Comparison of experimental and back-calculated spectra, including analysis of cross-peak buildup...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00454a005

    authors： Summers MF,South TL,Kim B,Hare DR

    更新日期：1990-01-16 00:00:00

  • Progesterone-binding components of chick oviduct: analysis of receptor structure by limited proteolysis.

    abstract：:An endogenous calcium-activated sulfhydryl protease in chick oviduct cytosol has been utilized to study the structure of the chick oviduct progesterone receptor subunits, progestophilins A (79 000 g/mol) and B (117 000 g/mol). The protease is not a normal component of the native progesterone receptor aggregate (6 and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00543a015

    authors： Vedeckis WV,Schrader WT,O'Malley BW

    更新日期：1980-01-22 00:00:00