Abstract:
:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such as clavulanic acid and the penam sulfones. Kinetic analysis of the interaction of the enzyme with the Z isomer has allowed a detailed evaluation of the factors that are important in the design of anti-beta-lactamase agents. In contrast to the Z compound, the E isomer of 6-(methoxymethylene)penicillanic acid is not a substrate, an inhibitor, or an inactivator of the enzyme.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Brenner DG,Knowles JRdoi
10.1021/bi00319a025subject
Has Abstractpub_date
1984-11-20 00:00:00pages
5839-46issue
24eissn
0006-2960issn
1520-4995journal_volume
23pub_type
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