Vinca site agents induce structural changes in tubulin different from and antagonistic to changes induced by colchicine site agents.

abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

journal_title：Biochemistry

authors： Skancke J,Bar N,Kuiper M,Hsu LM

更新日期：2015-07-21 00:00:00

abstract：:Carbamoyl phosphate synthetase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions occurring at three active sites connected by a molecular tunnel of 100 A. To understand the mechanism for coordination and synchronization among the active sites, the pre-steady-state time cour...

journal_title：Biochemistry

authors： Miles BW,Raushel FM

更新日期：2000-05-02 00:00:00

abstract：:Choline oxidase catalyzes the oxidation of choline to glycine betaine through a two-step, four-electron reaction with betaine aldehyde as an intermediate. Oxygen is the final electron acceptor. Alcohol oxidation is initiated by the removal of the substrate hydroxyl proton by an unknown active site residue with a pKa v...

journal_title：Biochemistry

authors： Smitherman C,Rungsrisuriyachai K,Germann MW,Gadda G

更新日期：2015-01-20 00:00:00

abstract：:Src homology 2 (SH2) domains are phosphotyrosine- (pY-) binding modules found in a variety of signal-transducing proteins and constitute an important class of drug targets for the treatment of signaling related diseases/conditions. To date, a large number of peptidic as well as nonpeptidic SH2 domain inhibitors have b...

journal_title：Biochemistry

authors： Park J,Fu H,Pei D

更新日期：2003-05-06 00:00:00

abstract：:A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...

journal_title：Biochemistry

authors： Urbatsch IL,al-Shawi MK,Senior AE

更新日期：1994-06-14 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...

journal_title：Biochemistry

authors： Danyal K,Dean DR,Hoffman BM,Seefeldt LC

更新日期：2011-11-01 00:00:00

abstract：:Ribonuclease T1 was crystallized in the presence of vanadate(V). The crystal structure was solved by molecular replacement and refined by least-squares methods using stereochemical restraints. The refinement was based on data between 10 and 1.8 A and converged at a crystallographic R factor of 0.137. Except for the su...

journal_title：Biochemistry

authors： Kostrewa D,Choe HW,Heinemann U,Saenger W

更新日期：1989-09-19 00:00:00

abstract：:Three electrochromic difference spectra induced by the deposition of (1) a negative charge on the primary quinone acceptor, Q(A), (2) a positive charge on (or near) Tyr161 of the D1 subunit (Y(Z)), and (3) a positive charge on the manganese cluster were determined at room temperature in photosystem II (PSII) core part...

journal_title：Biochemistry

authors： Mulkidjanian AY,Cherepanov DA,Haumann M,Junge W

更新日期：1996-03-05 00:00:00

abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...

journal_title：Biochemistry

authors： Childers MC,Daggett V

更新日期：2019-11-05 00:00:00

abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

journal_title：Biochemistry

authors： Maly P,Wower J,Zobawa M,Brimacombe R

更新日期：1983-06-21 00:00:00

abstract：:NMR spectroscopy and simulated annealing calculations have been used to determine the three-dimensional structure of RK-1, an antimicrobial peptide from rabbit kidney recently discovered from homology screening based on the distinctive physicochemical properties of the corticostatins/defensins. RK-1 consists of 32 res...

journal_title：Biochemistry

authors： McManus AM,Dawson NF,Wade JD,Carrington LE,Winzor DJ,Craik DJ

更新日期：2000-12-26 00:00:00

abstract：:Stopped-flow fluorescence experiments have been carried out to study the steady-state kinetics of hydrolysis of Leu-Gly-NHNH-Dns [Dns = 5-(dimethylamino)naphthalene-1-sulfonyl] by porcine kidney cytosol leucine aminopeptidase (LAP) in 50% v/v methanol/buffer solution at ambient temperature and the pre-steady-state kin...

journal_title：Biochemistry

authors： Lin WY,Lin SH,Morris RJ,Van Wart HE

更新日期：1988-07-12 00:00:00

abstract：:FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...

journal_title：Biochemistry

authors： Hou C,Tsodikov OV

更新日期：2015-12-22 00:00:00

abstract：:Currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. Among the many families of glycoside hydrol...

journal_title：Biochemistry

authors： Harris PV,Welner D,McFarland KC,Re E,Navarro Poulsen JC,Brown K,Salbo R,Ding H,Vlasenko E,Merino S,Xu F,Cherry J,Larsen S,Lo Leggio L

更新日期：2010-04-20 00:00:00

abstract：:The effect of low molecular-weight compounds on the equilibrium constant K(A) can be used to explore the energetics and molecular mechanism of protein-DNA interactions. Here we use the complex composed of the integrase Tn916 DNA-binding domain and its target DNA duplex to investigate the effects of salt and the nonion...

journal_title：Biochemistry

authors： Milev S,Bosshard HR,Jelesarov I

更新日期：2005-01-11 00:00:00

abstract：:The crystal structure of the complex between the quinoprotein methylamine dehydrogenase (MADH) and the type I blue copper protein amicyanin, both from Paracoccus denitrificans, has been determined at 2.5-A resolution using molecular replacement. The search model was MADH from Thiobacillus versutus. The amicyanin could...

journal_title：Biochemistry

authors： Chen L,Durley R,Poliks BJ,Hamada K,Chen Z,Mathews FS,Davidson VL,Satow Y,Huizinga E,Vellieux FM

更新日期：1992-06-02 00:00:00

abstract：:The L-type Ca(2+) channel Ca(v)1.2 forms macromolecular signaling complexes that comprise the β(2) adrenergic receptor, trimeric G(s) protein, adenylyl cyclase, and cAMP-dependent protein kinase (PKA) for efficient signaling in heart and brain. The protein phosphatases PP2A and PP2B are part of this complex. PP2A coun...

journal_title：Biochemistry

authors： Xu H,Ginsburg KS,Hall DD,Zimmermann M,Stein IS,Zhang M,Tandan S,Hill JA,Horne MC,Bers D,Hell JW

更新日期：2010-12-07 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:Phenylalanine acts as an allosteric activator of the tetrahydropterin-dependent enzyme phenylalanine hydroxylase. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into local conformational changes accompanying activation of rat phenylalanine hydroxylase by phenylalanine. Peptide...

journal_title：Biochemistry

authors： Li J,Dangott LJ,Fitzpatrick PF

更新日期：2010-04-20 00:00:00

abstract：:Monomolecular layers of undissociated bile acids and membrane lipids at the air/water interface serve as useful models for the interactions between fully ionized bile salts and physiological membranes. Employing an automated Langmuir-Pockels surface balance, surface pressures and dipole moments were measured as functi...

journal_title：Biochemistry

authors： Fahey DA,Carey MC,Donovan JM

更新日期：1995-08-29 00:00:00

abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...

journal_title：Biochemistry

authors： Danelius E,Andersson H,Jarvoll P,Lood K,Gräfenstein J,Erdélyi M

更新日期：2017-06-27 00:00:00

abstract：:Casein kinase II (CKII) is a ubiquitous protein kinase, found predominantly in cell nuclei, which has two subunits in a tetrameric alpha 2 beta 2 or alpha alpha' beta 2 conformation. The catalytic center is present in the alpha subunit which is active by itself while beta is a regulatory subunit that can greatly enhan...

journal_title：Biochemistry

authors： Hinrichs MV,Jedlicki A,Tellez R,Pongor S,Gatica M,Allende CC,Allende JE

更新日期：1993-07-20 00:00:00

abstract：:The arrangement of the mRNA on the Escherichia coli ribosome with respect to ribosomal RNA sites has been investigated by photochemical cross-linking experiments. mRNA analogues 51-54 nucleotides in length contained a Shine-Dalgarno sequence, a single codon for tRNA(Gly), and 4-thiouridine (s4U) in the 5' third of the...

journal_title：Biochemistry

authors： Bhangu R,Juzumiene D,Wollenzien P

更新日期：1994-03-15 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...

journal_title：Biochemistry

authors： Klapper DG,Goodfriend L,Capra JD

更新日期：1980-12-09 00:00:00

abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...

journal_title：Biochemistry

authors： Omann GM,Glaser M

更新日期：1984-10-09 00:00:00

abstract：:1-Aminocyclopropanephosphonate (ACPP) was synthesized, and its effects on the pyridoxal 5'-phosphate linked enzymes 1-aminocyclopropanecarboxylate (ACPC) deaminase from Pseudomonas sp. ACPC and alanine racemase from Bacillus stearothermophilus were studied. ACPP was found to be a potent inhibitor of both enzymes with ...

journal_title：Biochemistry

authors： Erion MD,Walsh CT

更新日期：1987-06-16 00:00:00

abstract：:Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990) Science (Washington, D.C.) 249, 1398-1405]. The availability of SeMet...

journal_title：Biochemistry

authors： Boles JO,Cisneros RJ,Weir MS,Odom JD,Villafranca JE,Dunlap RB

更新日期：1991-11-19 00:00:00

abstract：:Tropomyosin is postulated to confer stability to actin filaments in nonmuscle cells. We have found that a nonmuscle tropomyosin isolated from the intestinal epithelium can directly stabilize actin filaments by slowing depolymerization from the pointed, or slow-growing, filament end. Kinetics of elongation and depolyme...

journal_title：Biochemistry

authors： Broschat KO,Weber A,Burgess DR

更新日期：1989-10-17 00:00:00

abstract：:We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...

journal_title：Biochemistry

authors： Whitley P,Nilsson L,von Heijne G

更新日期：1993-08-24 00:00:00