Abstract：
:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agents and agricultural pesticides. No natural substrate has been identified for phosphotriesterase, and it has been suggested that the enzyme may have evolved the ability to hydrolyze synthetic compounds in bacteria under selective pressure to meet nutritional needs. PHP, which has 28% sequence identity with phosphotriesterase, may belong to the family of proteins from which phosphotriesterase evolved. Here we report the cloning, expression, initial characterization, and high-resolution X-ray crystallographic structure of PHP. Biochemical analysis shows that PHP is monomeric and binds two zinc ions per monomer. Unlike phosphotriesterase, PHP does not catalyze the hydrolysis of nonspecific phosphotriesters. The structure, similar to that of phosphotriesterase, consists of a long, elliptical alpha/beta barrel and has a binuclear zinc center in a cleft at the carboxy end of the barrel at the location of the presumptive active site.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Buchbinder JL,Stephenson RC,Dresser MJ,Pitera JW,Scanlan TS,Fletterick RJdoi
10.1021/bi971707+subject
Has Abstractpub_date
1998-04-14 00:00:00pages
5096-106issue
15eissn
0006-2960issn
1520-4995pii
bi971707+journal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00286
更新日期：2019-06-04 00:00:00
abstract：:Staphylococcus aureus is a leading cause of surgical site infections that results in increased hospital stays due to the development of chronic wounds. Little is known about factors involved in S. aureus' ability to prevent wounds from healing. We discovered a novel secreted protein produced by a surgical site isolate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00523
更新日期：2015-08-11 00:00:00
abstract：:The function of Lys-59, Arg-141, and Arg-277 in PAPS binding and catalysis of the flavonol 3-sulfotransferase was investigated. Affinity chromatography of conservative mutants with PAPS analogues allowed us to determine that Lys-59 interacts with the 5' portion of the nucleotide, while Arg-141 interacts with the 3' po...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982239m
更新日期：1999-03-30 00:00:00
abstract：:Ketopantoate reductase (EC 1.1.1.169) catalyzes the NADPH-dependent reduction of alpha-ketopantoate to form D-(-)-pantoate in the pantothenate/coenzyme A biosynthetic pathway. The enzyme encoded by the panE gene from E. coli K12 was overexpressed and purified to homogeneity. The native enzyme exists in solution as a m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992676g
更新日期：2000-04-04 00:00:00
abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00469a004
更新日期：1990-05-01 00:00:00
abstract：:Ferredoxin I from Desulfovibrio africanus (Da FdI) is a small acidic [4Fe-4S] cluster protein that exchanges electrons with pyruvate-ferredoxin oxidoreductase (PFOR), a key enzyme in the energy metabolism of anaerobes. The thermodynamic properties and the electron transfer between PFOR and either native or mutated FdI...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0485878
更新日期：2004-12-14 00:00:00
abstract：:Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010713+
更新日期：2001-09-25 00:00:00
abstract：:The effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 RT was determined using a 66 nucleotide RNA template containing a stable 12 base pair hairpin structure. Prior to reaching the hairpin structure, the primer elongation by RT was fast and the kinetics of polymerization was not aff...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971217h
更新日期：1997-10-14 00:00:00
abstract：:The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00142a009
更新日期：1992-07-14 00:00:00
abstract：:A recently discovered bifunctional antibiotic-resistance enzyme named AAC(3)-Ib/AAC(6')-Ib', from Pseudomonas aeruginosa, catalyzes acetylation of aminoglycoside antibiotics. Since both domains are acetyltransferases, each was cloned and purified for mechanistic studies. The AAC(3)-Ib domain appears to be highly speci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700111z
更新日期：2007-05-01 00:00:00
abstract：:The interaction of a divalent metal ion with a leaving 3' oxygen is a central component of several proposed mechanisms of phosphoryl transfer. In support of this are recent kinetic studies showing that thiophilic metal ions (e.g., Mn2+) stimulate the hydrolysis of compounds in which sulfur takes the place of the leavi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981537g
更新日期：1999-01-12 00:00:00
abstract：:Competitive inhibition of the monophenolase reaction of tyrosinase by a variety of compounds was investigated with respect to temperature and pH. Derivatives of benzoic acid as well as p-nitrophenol were the best inhibitors. Toluene and several N-heterocycles were all weak inhibitors. Thermodynamic parameters of tolue...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00185a010
更新日期：1994-05-17 00:00:00
abstract：:The microscopic interactions and mechanisms leading to nascent protein folding events are generally unknown. While such short time-scale events are difficult to study experimentally, molecular dynamics simulations of peptides can provide a useful model for studying events related to protein folding initiation. Recentl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00053a005
更新日期：1993-01-19 00:00:00
abstract：:Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00704
更新日期：2018-10-02 00:00:00
abstract：:The assignment of the 1H, 15N, 13CO, and 13C resonances of recombinant human interleukin-4 (IL-4), a protein of 133 residues and molecular mass of 15.4 kDa, is presented based on a series of 11 three-dimensional (3D) double- and triple-resonance heteronuclear NMR experiments. These studies employ uniformly labeled 15N...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00132a026
更新日期：1992-05-05 00:00:00
abstract：:Factor V(a) (FV(a)) is a cofactor for the serine protease factor X(a) that activates prothrombin to thrombin in the presence of Ca(2+) and a membrane surface. FV(a) is a heterodimer composed of one heavy chain (A1 and A2 domains) and one light chain (A3, C1, and C2 domains). We use fluorescence, circular dichroism, an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010449k
更新日期：2001-07-27 00:00:00
abstract：:We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500504y
更新日期：2014-09-09 00:00:00
abstract：:Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. The heme binding region has been localized to residues 1-385 of the beta1 subunit [beta1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972686m
更新日期：1998-03-31 00:00:00
abstract：:Phosphorylase kinase, a regulatory enzyme of glycogenolysis in skeletal muscle, is a hexadecameric oligomer consisting of four copies each of a catalytic subunit (gamma) and three regulatory subunits (alpha, beta, and delta, the last being endogenous calmodulin). The enzyme is activated by a variety of effectors actin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982060b
更新日期：1999-02-23 00:00:00
abstract：:Aminoacylation of tRNA by aminoacyl-tRNA synthetases is the essential reaction that matches protein amino acids with the trinucleotide sequences specified in mRNA. Direct electrostatic interactions made by tRNA synthetases with discriminating functional groups on the tRNA bases have long been known to determine aminoa...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi7014647
更新日期：2007-09-18 00:00:00
abstract：:Four-coordinate metalloporphyrins activate soluble guanylyl cyclase. Ni(II)PPIX and Cu(II)PPIX are high affinity activators, with activation constants of 24 and 17 nM, respectively. Both metalloporphyrins remain stably bound to the enzyme, enabling spectroscopic characterization of the Ni(II)- and Cu(II)-reconstituted...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010777k
更新日期：2002-08-06 00:00:00
abstract：:Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00520a035
更新日期：1981-08-18 00:00:00
abstract：:The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051550i
更新日期：2005-11-08 00:00:00
abstract：:NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00164a033
更新日期：1992-12-15 00:00:00
abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100293q
更新日期：2010-07-06 00:00:00
abstract：:The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi030065p
更新日期：2003-07-29 00:00:00
abstract：:The residue C221 on pyruvate decarboxylase (EC. 4.1.1.1) from Saccharomyces cerevisiae has been shown to be the site where the substrate activation cascade is triggered [Baburina et al. (1994) Biochemistry 33, 5630-5635] and is located on the beta domain [Arjunan et al. (1996) J. Mol. Biol. 256, 590], while the active...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9709912
更新日期：1998-02-03 00:00:00
abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201263n
更新日期：2011-10-25 00:00:00
abstract：:Monomolecular layers of undissociated bile acids and membrane lipids at the air/water interface serve as useful models for the interactions between fully ionized bile salts and physiological membranes. Employing an automated Langmuir-Pockels surface balance, surface pressures and dipole moments were measured as functi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00034a023
更新日期：1995-08-29 00:00:00
abstract：:G proteins play an important role in transmitting hormonal signals, and fluorescence techniques would be useful to study their cellular distribution and mechanisms. To prepare active fluorescent G protein Go/Gi or beta gamma subunits were reacted with fluorescein isothiocyanate (FITC) to label the alpha (F-alpha) and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00060a035
更新日期：1993-03-09 00:00:00
