FeNO structure in distal pocket mutants of myoglobin based on resonance Raman spectroscopy.

abstract：:The search for a rational method of developing an antimalarial vaccine (malaria caused by Plasmodium falciparum) consists of blocking receptor-ligand interaction. Conserved peptides derived from proteins involved in invasion and having strong red blood cell binding ability have thus been identified; immunization studi...

journal_title：Biochemistry

authors： Patarroyo ME,Alba MP,Vargas LE,Silva Y,Rosas J,Rodríguez R

更新日期：2005-05-10 00:00:00

abstract：:The crystal structure of the complex between the quinoprotein methylamine dehydrogenase (MADH) and the type I blue copper protein amicyanin, both from Paracoccus denitrificans, has been determined at 2.5-A resolution using molecular replacement. The search model was MADH from Thiobacillus versutus. The amicyanin could...

journal_title：Biochemistry

authors： Chen L,Durley R,Poliks BJ,Hamada K,Chen Z,Mathews FS,Davidson VL,Satow Y,Huizinga E,Vellieux FM

更新日期：1992-06-02 00:00:00

abstract：:Linker histones bind to the nucleosomes and linker DNA of chromatin fibers, causing changes in linker DNA structure and stabilization of higher order folded and oligomeric chromatin structures. Linker histones affect chromatin structure acting primarily through their approximately 100-residue C-terminal domain (CTD). ...

journal_title：Biochemistry

authors： Lu X,Hamkalo B,Parseghian MH,Hansen JC

更新日期：2009-01-13 00:00:00

abstract：:The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by me...

journal_title：Biochemistry

authors： Goto Y,Aki K

更新日期：1984-12-18 00:00:00

abstract：:A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...

journal_title：Biochemistry

authors： Benveniste K,Wilczek J,Ruggieri A,Stern R

更新日期：1976-02-24 00:00:00

abstract：:Green fluorescent protein and its variants are frequently used as Förster (fluorescence) resonance energy transfer (FRET) pairs to determine the proximity of protein domains. We prepared fusion proteins comprising yellow fluorescent protein-Dictyostelium myosin II motor domain-cyan fluorescent protein (YFP-myosin-CFP)...

journal_title：Biochemistry

authors： Zeng W,Seward HE,Málnási-Csizmadia A,Wakelin S,Woolley RJ,Cheema GS,Basran J,Patel TR,Rowe AJ,Bagshaw CR

更新日期：2006-09-05 00:00:00

abstract：:PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...

journal_title：Biochemistry

authors： Jiang P,Ninfa AJ

更新日期：2009-12-08 00:00:00

abstract：:alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of 1-fucose from the donor sugar guanosine 5'-diphospho-beta-1-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-2H]-beta-1-fucose (GDP-[1-2H]-Fuc) as the substrate was observed...

journal_title：Biochemistry

authors： Murray BW,Wittmann V,Burkart MD,Hung SC,Wong CH

更新日期：1997-01-28 00:00:00

abstract：:We have investigated the kinetic and thermodynamic properties of carbon monoxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from the hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis of CO from heme a(3), the complex recombines with an apparent rate constant of approximately 3...

journal_title：Biochemistry

authors： Aagaard A,Gilderson G,Gomes CM,Teixeira M,Brzezinski P

更新日期：1999-08-03 00:00:00

abstract：:Comparative sequence analysis has successfully predicted secondary structure and tertiary interactions in ribosomal and other RNAs. Experiments presented here ask whether the scope of comparative sequence-based predictions can be extended to specific interactions between proteins and RNA, using as a system the well-ch...

journal_title：Biochemistry

authors： GuhaThakurta D,Draper DE

更新日期：1999-03-23 00:00:00

abstract：:Activation of washed human platelets initiated with alpha-thrombin, SFLLRN, or AYPGKF invariably results in the generation of PAR-1-(1-41) and PAR-4-(1-47). PAR-1-(1-41) and PAR-4-(1-47) are amino-terminal peptides generated when PAR-1 and -4 are cleaved in their first extracellular domains after R(41) and R(47), resp...

journal_title：Biochemistry

authors： Ofosu FA,Dewar L,Song Y,Cedrone AC,Hortelano G,Craven SJ

更新日期：2009-02-24 00:00:00

abstract：:Galactolipids are characteristic lipids of the photosynthetic membranes. They are highly enriched in the chloroplast and are present in photosystem structures. There are two major types of galactolipids, i.e., monogalactosyldiacylglycerol and digalactosyldiacylglycerol (DGDG) in chloroplastic membranes, which amount t...

journal_title：Biochemistry

authors： Fu B,Brown C,Mäler L

更新日期：2020-03-03 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

journal_title：Biochemistry

authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

更新日期：1994-09-06 00:00:00

abstract：:While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...

journal_title：Biochemistry

authors： Xin X,Xi L,Tu SC

更新日期：1994-10-11 00:00:00

abstract：:Activation enthalpies and entropies are reported for proton-deuteron exchange at 42 amide sites in T4 lysozyme and compared with activation volumes for the same residues obtained earlier [Hitchens, T. K., and Bryant, R. G. (1998) Biochemistry 37, 5878-5887]. There is no correlation found between activation volume and ...

journal_title：Biochemistry

authors： Dixon ME,Hitchens TK,Bryant RG

更新日期：2000-01-11 00:00:00

abstract：:Perioperative bleeding is a cause of major blood loss and is associated with increased rates of postoperative morbidity and mortality. To combat this, antifibrinolytic inhibitors of the serine protease plasmin are commonly used to reduce bleeding during surgery. The most effective and previously widely used of these i...

journal_title：Biochemistry

authors： Swedberg JE,Harris JM

更新日期：2011-10-04 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are ubiquitous in all three kingdoms of life and are implicated in many clinically relevant physiological processes. They couple the energy released by ATP hydrolysis to facilitate substrate translocation across cell membranes. The crystal structures of type II ABC importers hav...

journal_title：Biochemistry

authors： Pan C,Weng J,Wang W

更新日期：2016-12-13 00:00:00

abstract：:Argonaute (AGO) proteins play central roles in nucleic acid-guided interference that regulates gene expression and defend against foreign genetic elements in all life. Although much progress has been made with respect to the function of argonaute proteins in target recognition and cleavage, the detailed mechanism of t...

journal_title：Biochemistry

authors： Song G,Chen H,Sheng G,Wang Y,Lou J

更新日期：2018-04-17 00:00:00

abstract：:A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those...

journal_title：Biochemistry

authors： Jaschke PR,Beatty JT

更新日期：2007-10-30 00:00:00

abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...

journal_title：Biochemistry

authors： Moffatt NS,Bruinsma E,Uhl C,Obermann WM,Toft D

更新日期：2008-08-05 00:00:00

abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...

journal_title：Biochemistry

authors： Kingsbury NL,Renegar RH,Chalovich JM

更新日期：2013-10-29 00:00:00

abstract：:A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yie...

journal_title：Biochemistry

authors： Mahoney WC,Hermodson MA

更新日期：1979-08-21 00:00:00

abstract：:1-Bromovinyl (I), Z-2-bromovinyl (II), 1,2-dibromoethyl (III), and a series of 4-(halomethyl)-2-nitrophenyl (IVa-c) diethyl phosphate esters were examined as substrates and mechanism-based inhibitors for the bacterial phosphotriesterase. All of these compounds were found to act as substrates for the enzyme. Inhibitor ...

journal_title：Biochemistry

authors： Hong SB,Mullins LS,Shim H,Raushel FM

更新日期：1997-07-22 00:00:00

abstract：:Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...

journal_title：Biochemistry

authors： Haque ME,McIntosh TJ,Lentz BR

更新日期：2001-04-10 00:00:00

abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...

journal_title：Biochemistry

authors： Ichimaru N,Murai M,Abe M,Hamada T,Yamada Y,Makino S,Nishioka T,Makabe H,Makino A,Kobayashi T,Miyoshi H

更新日期：2005-01-18 00:00:00

abstract：:Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...

journal_title：Biochemistry

authors： Chowdhary PK,Keshavan N,Nguyen HQ,Peterson JA,González JE,Haines DC

更新日期：2007-12-18 00:00:00

abstract：:31P NMR measurements were made (at 121.5 MHz and 5 degrees C) on enzyme-bound substrate complexes of 3-phosphoglycerate kinase in order to address three questions pertaining to (i) the integrity of the enzyme-substrate complexes with Mg(II) in the presence of sulfate concentrations typical of those used for crystalliz...

journal_title：Biochemistry

authors： Ray BD,Rao BD

更新日期：1988-07-26 00:00:00

abstract：:A mechanistic study of rat liver alpha-(2-->6) sialyltransferase (ST) is presented that includes isotope trapping experiments and kinetic isotope effects on V/K for the ST-catalyzed reaction of isotopically labeled CMP-N-acetylneuraminate and N-acetyllactosamine. The isotope trapping experiments confirmed that the kin...

journal_title：Biochemistry

authors： Bruner M,Horenstein BA

更新日期：1998-01-06 00:00:00

abstract：:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...

journal_title：Biochemistry

authors： Prevelige PE Jr,Fasman GD

更新日期：1987-05-19 00:00:00

abstract：:Treatment of soybean lipoxygenase isozyme 1 with its substrates, linoleic acid and oxygen, or product, 13(S)-hydroperoxy-9,11(Z,E)-octadecadienoic acid (13-HPOD), leads to the appearance of a purple color. Although the structure of the chromophore has not been determined, we present strong evidence that it is an Fe(3+...

journal_title：Biochemistry

authors： Nelson MJ,Chase DB,Seitz SP

更新日期：1995-05-09 00:00:00