当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Comparisons of pressure and temperature activation parameters for amide hydrogen exchange in T4 lysozyme.

Comparisons of pressure and temperature activation parameters for amide hydrogen exchange in T4 lysozyme.

Abstract：

:Activation enthalpies and entropies are reported for proton-deuteron exchange at 42 amide sites in T4 lysozyme and compared with activation volumes for the same residues obtained earlier [Hitchens, T. K., and Bryant, R. G. (1998) Biochemistry 37, 5878-5887]. There is no correlation found between activation volume and activation entropy or activation enthalpy. The activation enthalpy is linearly related to the activation entropy in part as a consequence of a relatively narrow sampling window for the rate constants that corresponds to a narrow range of activation free energy. A consequence of the entropy-enthalpy compensation is preservation of rank order of proton exchange. Variations in DeltaH, DeltaS, and DeltaV for residues that are structurally close together in the folded protein suggest that there may be a variety of energetically distinct pathways for the access of solvent to these structurally related exchange sites.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Dixon ME,Hitchens TK,Bryant RG

doi

10.1021/bi991718y

subject

Has Abstract

pub_date

2000-01-11 00:00:00

pages

248-54

issue

1

eissn

0006-2960

issn

1520-4995

pii

bi991718y

journal_volume

39

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Substitution of Tyr254 with Phe at the active site of flavocytochrome b2: consequences on catalysis of lactate dehydrogenation.

    abstract：:A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F., & Mathews, F.S. (1987) in Flavins and Flavoprotein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00479a008

    authors： Dubois J,Chapman SK,Mathews FS,Reid GA,Lederer F

    更新日期：1990-07-10 00:00:00

  • The effect of O-fucosylation on the first EGF-like domain from human blood coagulation factor VII.

    abstract：:The first epidermal growth factor-like domain (EGF-1) from blood coagulation factor VII (FVII) contains two unusual O-linked glycosylation sites at Ser-52 and Ser-60. We report here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, its Ca2+-binding affinity, and its interaction...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990234z

    authors： Kao YH,Lee GF,Wang Y,Starovasnik MA,Kelley RF,Spellman MW,Lerner L

    更新日期：1999-06-01 00:00:00

  • A closer look at the spectroscopic properties of possible reaction intermediates in wild-type and mutant (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase.

    abstract：:(E)-4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH or LytB) catalyzes the terminal step of the MEP/DOXP pathway where it converts (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into the two products, isopentenyl diphosphate and dimethylallyl diphosphate. The reaction involves the reductive elimination ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi3001215

    authors： Xu W,Lees NS,Hall D,Welideniya D,Hoffman BM,Duin EC

    更新日期：2012-06-19 00:00:00

  • Ca2+-calmodulin regulates fesselin-induced actin polymerization.

    abstract：:Fesselin is a proline-rich actin-binding protein that was isolated from avian smooth muscle. Fesselin bundles actin and accelerates actin polymerization by facilitating nucleation. We now show that this polymerization of actin can be regulated by Ca(2+)-calmodulin. Fesselin was shown to bind to immobilized calmodulin ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0487490

    authors： Schroeter M,Chalovich JM

    更新日期：2004-11-02 00:00:00

  • Bis(pyrrolecarboxamide) linked to intercalating chromophore oxazolopyridocarbazole (OPC): selective binding to DNA and polynucleotides.

    abstract：:We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00220a029

    authors： Subra F,Carteau S,Pager J,Paoletti J,Paoletti C,Auclair C,Mrani D,Gosselin G,Imbach JL

    更新日期：1991-02-12 00:00:00

  • Roles of individual domains of annexin I in its vesicle binding and vesicle aggregation: a comprehensive mutagenesis study.

    abstract：:To understand the mechanism by which annexin I induces membrane aggregation, a comprehensive mutagenesis of all six Ca2+-binding sites was performed. When the cap residues of type II Ca2+-binding sites were systematically mutated to Ala, a type II site in domain II was shown to be essential for Ca2+-dependent vesicle ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980479w

    authors： Bitto E,Cho W

    更新日期：1998-07-14 00:00:00

  • Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product.

    abstract：:The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00443a008

    authors： Rusnak F,Faraci WS,Walsh CT

    更新日期：1989-08-22 00:00:00

  • Kinetic, Raman, NMR, and site-directed mutagenesis studies of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-CoA thioesterase active site.

    abstract：:4-Hydroxybenzoyl-coenzyme A (4-HBA-CoA) thioesterase catalyzes the hydrolysis of 4-HBA-CoA to 4-hydroxybenzoate and CoA. X-ray crystallographic analysis of the liganded enzyme has shown that the benzoyl thioester and pantetheine moieties of the substrate ligand are bound in a narrow crevice while the nucleotide moiety...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0262303

    authors： Zhuang Z,Song F,Zhang W,Taylor K,Archambault A,Dunaway-Mariano D,Dong J,Carey PR

    更新日期：2002-09-17 00:00:00

  • Calcium and membrane-binding properties of monomeric and multimeric annexin II.

    abstract：:The calcium-dependent interaction of several annexins with membranes was studied. A novel technique was developed that allowed estimation of calcium binding to aggregating systems. This consisted of immobilized phospholipids on phenyl-Sepharose. Proteins associated with this affinity gel in the presence of radioactive...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00249a009

    authors： Evans TC Jr,Nelsestuen GL

    更新日期：1994-11-15 00:00:00

  • Effect of protein-protein interaction on light adaptation of bacteriorhodopsin.

    abstract：:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00555a043

    authors： Casadio R,Stoeckenius W

    更新日期：1980-07-08 00:00:00

  • Isolation and characterization of rat pancreatic elastase.

    abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00285a008

    authors： Largman C

    更新日期：1983-08-02 00:00:00

  • Nuclear magnetic resonance studies of 2'- and 3'-ribonucleotide structures in solution.

    abstract：:A systematic 220-MHz proton nuclear magnetic resonance (nmr) study has been made of all common purine and pyrimidine 2'(3')-ribonucleotides in D2O solutions at 20 plus or minus 2 degrees. Spectra for the entire series were measured under similar conditions of concentration, temperature, and ionic strength, thereby fac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00674a013

    authors： Davies DB,Danyluk SS

    更新日期：1975-02-11 00:00:00

  • Influence of cofactor pyridoxal 5'-phosphate on reversible high-pressure denaturation of isolated beta 2 dimer of tryptophan synthase bienzyme complex from Escherichia coli.

    abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00323a016

    authors： Seifert T,Bartholmes P,Jaenicke R

    更新日期：1985-01-15 00:00:00

  • In vitro biosynthesis of a decasaccharide prototype of multiply branched polylactosaminoglycan backbones.

    abstract：:Multiply branched polylactosaminoglycans are expressed in glycoproteins and glycolipids of many cells. Interest in their biology stems from their abundant expression in early embryonal cells and from their ability to carry multiple lectin-binding determinants, which makes them prominent ligands and antagonists of cell...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9627673

    authors： Leppanen A,Salminen H,Zhu Y,Maaheimo H,Helin J,Costello CE,Renkonen O

    更新日期：1997-06-10 00:00:00

  • Hydrogen bonding networks tune proton-coupled redox steps during the enzymatic six-electron conversion of nitrite to ammonia.

    abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500854p

    authors： Judd ET,Stein N,Pacheco AA,Elliott SJ

    更新日期：2014-09-09 00:00:00

  • Mutation spectra of M13 vectors containing site-specific Cis-Syn, Trans-Syn-I, (6-4), and Dewar pyrimidone photoproducts of thymidylyl-(3'-->5')-thymidine in Escherichia coli under SOS conditions.

    abstract：:The mutations spectra of cis-syn, trans-syn-I, (6-4), and Dewar pyrimidone photoproducts of the TT site of AATTAA and TATTAT in the (-) strand of a heteroduplex M13 vector were obtained in an excision and photoreversal repair deficient Escherichia coli host under SOS conditions. Oligonucleotides containing site-specif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951975c

    authors： Smith CA,Wang M,Jiang N,Che L,Zhao X,Taylor JS

    更新日期：1996-04-02 00:00:00

  • Membrane permeabilization induced by cytolytic delta-endotoxin CytA from Bacillus thuringiensis var. israelensis.

    abstract：:CytA is a member of a functionally defined family of insecticidal delta-endotoxins occurring in parasporal crystals of Bacillus thuringiensis var. israelensis. We investigated the ability of CytA to permeabilize the membrane and release fluorescence marker molecules from unilamellar lipid vesicles. Both protoxin (27 k...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960970s

    authors： Butko P,Huang F,Pusztai-Carey M,Surewicz WK

    更新日期：1996-09-03 00:00:00

  • DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

    abstract：:Reaction of poly(dA-[2'S-3H]dU) with activated bleomycin yields [3H]uracil propenal that completely retains the tritium label. In contrast, we have previously shown that reaction of poly(dA-[2'R-3H]dU) with activated bleomycin affords unlabeled uracil propenal [Wu, J. C., Kozarich, J. W., & Stubbe, J. (1983) J. Biol. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a037

    authors： Ajmera S,Wu JC,Worth L Jr,Rabow LE,Stubbe J,Kozarich JW

    更新日期：1986-10-21 00:00:00

  • The binding of the PDZ tandem of syntenin to target proteins.

    abstract：:PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052225y

    authors： Grembecka J,Cierpicki T,Devedjiev Y,Derewenda U,Kang BS,Bushweller JH,Derewenda ZS

    更新日期：2006-03-21 00:00:00

  • Polymerization properties of the Thermotoga maritima actin MreB: roles of temperature, nucleotides, and ions.

    abstract：:MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its cellular functions, we know very little about the biochemical behavior of MreB. We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701538e

    authors： Bean GJ,Amann KJ

    更新日期：2008-01-15 00:00:00

  • Electrostatic Repulsion during Ferritin Assembly and Its Screening by Ions.

    abstract：:Escherichia coli non-heme-binding ferritin A (EcFtnA) is a spherical cagelike protein that is composed of 24 identical subunits. EcFtnA dissociates into 2-mers under acidic conditions and can reassemble into the native structure when the pH is increased. To understand how electrostatic interactions influence the assem...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01197

    authors： Sato D,Takebe S,Kurobe A,Ohtomo H,Fujiwara K,Ikeguchi M

    更新日期：2016-01-26 00:00:00

  • Laser flash photolysis studies of the kinetics of reduction of spinach and Clostridium ferredoxins by a viologen analogue: electrostatically controlled nonproductive complex formation and differential reactivity among the iron-sulfur clusters.

    abstract：:We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00440a050

    authors： Navarro JA,Cheddar G,Tollin G

    更新日期：1989-07-11 00:00:00

  • Mapping the bimolecular interface of the parathyroid hormone (PTH)-PTH1 receptor complex: spatial proximity between Lys(27) (of the hormone principal binding domain) and leu(261) (of the first extracellular loop) of the human PTH1 receptor.

    abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000195n

    authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

    更新日期：2000-07-18 00:00:00

  • Pre-steady-state kinetic analysis of the trichodiene synthase reaction pathway.

    abstract：:The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi963018o

    authors： Cane DE,Chiu HT,Liang PH,Anderson KS

    更新日期：1997-07-08 00:00:00

  • The amino acid sequence of chick skin collagen alpha1-CB7.

    abstract：:The amino acid sequence of chick skin collagen alpha1-CB7, the 268 CNBr peptide from the helical portion of the alpha1 chain, has been determined by automatic and manual degradation of tryptic and chymotryptic peptides, and of the COOH-terminal fragment produced by cleavage with animal collagenase. The resulting seque...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00684a013

    authors： Highberger JH,Corbett C,Kang AH,Gross J

    更新日期：1975-07-01 00:00:00

  • Effects of site-directed mutations on heme reduction in Escherichia coli nitrate reductase A by menaquinol: a stopped-flow study.

    abstract：:We have studied the effects of site-directed mutations in Escherichia coli nitrate reductase A (NarGHI) on heme reduction by a menaquinol analogue (menadiol) using the stopped-flow method. For NarGHI(H66Y) and NarGHI(H187Y), both lacking heme b(L) but having heme b(H), the heme reduction by menadiol is abolished. For ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0350856

    authors： Zhao Z,Rothery RA,Weiner JH

    更新日期：2003-12-09 00:00:00

  • Partial purification and characterization of a neutral protease which cleaves type IV collagen.

    abstract：:A neutral protease has been extracted from the media of cultured metastatic tumor cells and purified approximately 1000 times after sequential ammonium sulfate fractionization, concanavalin A column chromatography, and molecular sieve chromatography. The protease has an apparent molecular weight of 70 000--80 000, is ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00504a017

    authors： Liotta LA,Tryggvason K,Garbisa S,Robey PG,Abe S

    更新日期：1981-01-06 00:00:00

  • Structure, topology, and tilt of cell-signaling peptides containing nuclear localization sequences in membrane bilayers determined by solid-state NMR and molecular dynamics simulation studies.

    abstract：:Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061895g

    authors： Ramamoorthy A,Kandasamy SK,Lee DK,Kidambi S,Larson RG

    更新日期：2007-01-30 00:00:00

  • Mechanistic studies of the oxygen-mediated oxidation of nitrosylhemoglobin.

    abstract：:Nitrosylhemoglobin (HbFe(II)NO) has been shown to be generated in vivo from the reaction of deoxyHb with NO(*) as well as with nitrite. Despite the physiological importance attributed to this form of Hb, its reactivity has not been investigated in detail. In this study, we showed that the rate of oxidation of HbFe(II)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0475929

    authors： Herold S,Röck G

    更新日期：2005-04-26 00:00:00

  • Binding of T4 endonuclease V to deoxyribonucleic acid irradiated with ultraviolet light.

    abstract：:Endonuclease V of bacteriophage T4 binds to UV-irradiated deoxyribonucleic acid (DNA) but not to unirradiated DNA. We have developed an assay to detect this binding, based on the retention of enzyme--DNA complexes on nitrocellulose filters. The amount of complex retained, ascertained by using radioactive DNA, is a mea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00549a026

    authors： Seawell PC,Simon TJ,Ganesan AK

    更新日期：1980-04-15 00:00:00