The amino acid sequence of chick skin collagen alpha1-CB7.

abstract：:Many investigations have revealed that ribosome numbers increase in parallel with the growth rate of cells. Here we show that the absolute level of protein synthesis may not be the only factor influencing rRNA synthesis in a nondividing eukaryotic cell. Under conditions of complete (greater than 99%) inhibition of pro...

journal_title：Biochemistry

authors： Yamamoto KK,Pellegrini M

更新日期：1990-12-18 00:00:00

abstract：:Interconversion of protein conformations is imperative to function, as evidenced by conformational changes associated with enzyme catalytic cycles, ligand binding and post-translational modifications. In this study, we used 15N NMR relaxation experiments to probe the fast (i.e., ps-ns) and slow (i.e., micros-ms) confo...

journal_title：Biochemistry

authors： Henkels CH,Chang YC,Chamberlin SI,Oas TG

更新日期：2007-12-25 00:00:00

abstract：:Recently, we revealed the architecture of the clamp-loader-helicase (tau-DnaB) complex in Bacillus by atomic force microscopy imaging and constructed a structural model, whereby a pentameric clamp-loader interacts with the hexameric helicase. Crucial to this model is the assumption that the clamp-loader forms a pentam...

journal_title：Biochemistry

authors： Haroniti A,Till R,Smith MC,Soultanas P

更新日期：2003-09-23 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) reversibly transfers an acetyl group between CoA and the 3-hydroxyl of either chloramphenicol (Cm) or 1-acetylchloramphenicol (1AcCm). The products of the forward reactions, 3-acetylchloramphenicol (3-AcCm) and 1,3-diacetylchloramphenicol (1,3Ac2-Cm), are the substrates for the ...

journal_title：Biochemistry

authors： Murray IA,Derrick JP,White AJ,Drabble K,Wharton CW,Shaw WV

更新日期：1994-08-23 00:00:00

abstract：:The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical propertie...

journal_title：Biochemistry

authors： Sheffield MJ,Baker BL,Li D,Owen NL,Baker ML,Bell JD

更新日期：1995-06-20 00:00:00

abstract：:The enzyme hydrogenase, from the photosynthetic bacterium Chromatium, was purified to homogeneity after solubilization of the particulate enzyme with deoxycholate. The purification procedure included ammonium sulfate fractionation, treatment with manganous phosphate gel, heating at 63 degrees, DEAE-cellulose chromatog...

journal_title：Biochemistry

authors： Gitlitz PH,Krasna AI

更新日期：1975-06-17 00:00:00

abstract：:Slow conformational changes have been proposed to be responsible for the kinetic positive cooperativity of glucokinase (GK) with glucose. Induced-fit and preexisting equilibrium kinetic models have been previously suggested. In the present study, equilibrium and pre-steady-state fluorescence spectroscopy has been used...

journal_title：Biochemistry

authors： Antoine M,Boutin JA,Ferry G

更新日期：2009-06-16 00:00:00

abstract：:Previously [Luz et al. (1994) Biochemistry 33, 7239-7249], we determined that Cl(-)- and -HCO3-dependent pHi homeostasis was perturbed in multidrug resistant (MDR) cells created by transfecting LR73 Chinese hamster ovary fibroblasts with wild-type mu (murine) MDR 1 (Gros et al., 1991). Via single-cell photometry exper...

journal_title：Biochemistry

authors： Roepe PD,Weisburg JH,Luz JG,Hoffman MM,Wei LY

更新日期：1994-09-13 00:00:00

abstract：:Specific interactions of human melanocortin-4 receptor (hMC4R) with its nonpeptide and peptide agonists were studied using alanine-scanning mutagenesis. The binding affinities and potencies of two synthetic, small-molecule agonists (THIQ, MB243) were strongly affected by substitutions in transmembrane alpha-helices (T...

journal_title：Biochemistry

authors： Pogozheva ID,Chai BX,Lomize AL,Fong TM,Weinberg DH,Nargund RP,Mulholland MW,Gantz I,Mosberg HI

更新日期：2005-08-30 00:00:00

abstract：:Curcumin potentiates the phosphorylation-dependent activity of human cystic fibrosis transmembrane conductance regulator (CFTR) in Fe(3+)-dependent and Fe(3+)-independent manners. Although the Fe(3+)-dependent curcumin potentiation results from removal of endogenous inhibitory Fe(3+) at the interface of the regulatory...

journal_title：Biochemistry

authors： Wang G

更新日期：2015-05-12 00:00:00

abstract：:The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as a conduit for the entrance and exit of the DNA. Here, the in vitro unfolding/refolding processes of the portal protein of P22 were investigated at different temperatures (1, 25, and 37 degrees C) through the use of ur...

journal_title：Biochemistry

authors： Braga CA,Carvalho D,Lara FA,Cortines JR,Moore SD,Prevelige PE Jr,Foguel D

更新日期：2007-06-26 00:00:00

abstract：:ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystal...

journal_title：Biochemistry

authors： Cupp-Vickery JR,Igarashi RY,Perez M,Poland M,Meyer CR

更新日期：2008-04-15 00:00:00

abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...

journal_title：Biochemistry

authors： Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti W

更新日期：2012-09-18 00:00:00

abstract：:The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...

journal_title：Biochemistry

authors： Keller D,Clausen R,Josefsen K,Led JJ

更新日期：2001-09-04 00:00:00

abstract：:The aromatic residues of fd coat protein in sodium dodecyl sulfate micelles are characterized by 1H and 13C NMR. Resonances from both types of nuclei show structure-induced chemical shift dispersion and line widths indicative of a folded native structure for the protein. The two tyrosines were found to have pKas of 12...

journal_title：Biochemistry

authors： Cross TA,Opella SJ

更新日期：1981-01-20 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem ...

journal_title：Biochemistry

authors： Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

更新日期：2015-09-29 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

journal_title：Biochemistry

authors： Truong TH,Carroll KS

更新日期：2012-12-18 00:00:00

abstract：:beta-amyloid peptide (Abeta) is the primary constituent of senile plaques, a defining feature of Alzheimer's disease. Aggregated Abeta is toxic to neurons, but the mechanism of toxicity remains unproven. One proposal is that Abeta toxicity results from relatively nonspecific Abeta-membrane interactions. We hypothesize...

journal_title：Biochemistry

authors： Kremer JJ,Pallitto MM,Sklansky DJ,Murphy RM

更新日期：2000-08-22 00:00:00

abstract：:Conformational changes in the prion protein cause transmissible spongiform encephalopathies, also referred to as prion diseases. In its native state, the prion protein is innocuous (PrPC), but it can misfold into a neurotoxic and infectious isoform (PrPSc). The full-length cellular form of the prion protein consists o...

journal_title：Biochemistry

authors： DeMarco ML,Daggett V

更新日期：2007-03-20 00:00:00

abstract：:The interaction between eosin-5-maleimide (EMA), an inhibitor of the anion transport protein, band 3, and I-, a transportable substrate, was investigated by fluorescence quenching. The Stern-Volmer plot for the quenching reaction between EMA-labeled band 3 and I- exhibits downward curvature both in human erythrocyte g...

journal_title：Biochemistry

authors： Pan RJ,Cherry RJ

更新日期：1995-04-11 00:00:00

abstract：:The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...

journal_title：Biochemistry

authors： Yeagle PL,Martin RB,Pottenger L,Langdon RG

更新日期：1978-07-11 00:00:00

abstract：:Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds having the generalized formula M(a)NH2(CH)4NH2M(b) are shown to form specific DNA lesions which can efficiently cross-link proteins to DNA. In this study, the homodinuclear case is represented by M(a) = M(b) = [cis-Pt(Cl2)-(NH3)] and the heterodinuclear...

journal_title：Biochemistry

authors： Van Houten B,Illenye S,Qu Y,Farrell N

更新日期：1993-11-09 00:00:00

abstract：:We have visualized by freeze-etch electron microscopy the macromolecular complexes of complement, C5b-8 and C5b-9, respectively, assembled on synthetic phospholipid bilayers. These complexes were formed sequentially by using purified human complement components C5b-6 followed by C7, C8, and C9. Complexes of C5b-8 were...

journal_title：Biochemistry

authors： McCloskey MA,Dankert JR,Esser AF

更新日期：1989-01-24 00:00:00

abstract：:According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...

journal_title：Biochemistry

authors： Kim HW,Shen TJ,Ho NT,Zou M,Tam MF,Ho C

更新日期：1996-05-28 00:00:00

abstract：:8-OxoGua (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to correspondi...

journal_title：Biochemistry

authors： Ito R,Hayakawa H,Sekiguchi M,Ishibashi T

更新日期：2005-05-03 00:00:00

abstract：:The dynamics of individual DNA molecules undergoing orthogonal field alternating gel electrophoresis (OFAGE) have been studied by use of T2 DNA molecules labeled with a dye and visualized with a fluorescence microscope. The mechanism of reorientation used by a molecule to align itself in the direction of the new ortho...

journal_title：Biochemistry

authors： Gurrieri S,Rizzarelli E,Beach D,Bustamante C

更新日期：1990-04-03 00:00:00

abstract：:Diphtheria toxin (DT) has been studied as a model for understanding active-site structure and function in the ADP-ribosyltransferases. Earlier evidence suggested that histidine-21 of DT is important for the ADP-ribosylation of eukaryotic elongation factor 2 (EF-2). We have generated substitutions of this residue by ca...

journal_title：Biochemistry

authors： Blanke SR,Huang K,Wilson BA,Papini E,Covacci A,Collier RJ

更新日期：1994-05-03 00:00:00

abstract：:Endonuclease V of bacteriophage T4 binds to UV-irradiated deoxyribonucleic acid (DNA) but not to unirradiated DNA. We have developed an assay to detect this binding, based on the retention of enzyme--DNA complexes on nitrocellulose filters. The amount of complex retained, ascertained by using radioactive DNA, is a mea...

journal_title：Biochemistry

authors： Seawell PC,Simon TJ,Ganesan AK

更新日期：1980-04-15 00:00:00

abstract：:Nitridomanganese(V) protoporphyrin IX was prepared by hypochlorite oxidation of the corresponding manganese(III) protoporphyrin IX derivative in the presence of ammonium ion and by photolysis of the corresponding azidomanganese(III) complex. Myoglobin and horseradish peroxidase containing this novel protoporphyrin der...

journal_title：Biochemistry

authors： Tsubaki M,Hori H,Hotta T,Hiwatashi A,Ichikawa Y,Yu NT

更新日期：1987-08-11 00:00:00

abstract：:Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

journal_title：Biochemistry

authors： Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

更新日期：2010-08-03 00:00:00