当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Extreme Expression of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Breast Cancer As Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry.

Extreme Expression of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Breast Cancer As Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry.

Abstract：

:Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA repair protein and plays other important roles. Increased levels of APE1 in cancer have been reported. However, available methods for measuring APE1 levels are indirect and not quantitative. We previously developed an approach using liquid chromatography and tandem mass spectrometry with isotope dilution to accurately measure APE1 levels. Here, we applied this methodology to measure APE1 levels in normal and cancerous human breast tissues. Extreme expression of APE1 in malignant tumors was observed, suggesting that breast cancer cells may require APE1 for survival. Accurate measurement of APE1 may be essential for the development of novel treatment strategies and APE1 inhibitors as anticancer drugs.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Coskun E,Jaruga P,Reddy PT,Dizdaroglu M

doi

10.1021/acs.biochem.5b00928

subject

Has Abstract

pub_date

2015-09-29 00:00:00

pages

5787-90

issue

38

eissn

0006-2960

issn

1520-4995

journal_volume

54

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Analysis of the kinetics of ovine follitropin agonist-antagonist interactions with pig ovarian membranes.

    abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00386a059

    authors： Sebok K,De Lean A,Sairam MR

    更新日期：1987-06-16 00:00:00

  • Crystal structure of recombinant murine adipocyte lipid-binding protein.

    abstract：:Adipocyte lipid-binding protein (ALBP) is the adipocyte member of an intracellular hydrophobic ligand-binding protein family. ALBP is phosphorylated by the insulin receptor kinase upon insulin stimulation. The crystal structure of recombinant murine ALBP has been determined and refined to 2.5 A. The final R factor for...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00128a024

    authors： Xu Z,Bernlohr DA,Banaszak LJ

    更新日期：1992-04-07 00:00:00

  • Proximity of periplasmic loops in the lactose permease of Escherichia coli determined by site-directed cross-linking.

    abstract：:Out of over 60 single-Cys mutants in putative periplasmic loops in lactose permease, three mutants [Tyr101 --> Cys (loop III/IV), Leu313 --> Cys (loop IX/X), and Ser375 --> Cys (loop XI/XII)] spontaneously form disulfide-linked dimers, indicating that these loops are located on the periphery of the 12-helix bundle tha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971172k

    authors： Sun J,Kaback HR

    更新日期：1997-09-30 00:00:00

  • Melting behavior of a covalently closed, single-stranded, circular DNA.

    abstract：:We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00427a037

    authors： Erie DA,Jones RA,Olson WK,Sinha NK,Breslauer KJ

    更新日期：1989-01-10 00:00:00

  • Mutational analysis of C-lobe ligands of human serum transferrin: insights into the mechanism of iron release.

    abstract：:Each homologous lobe of human serum transferrin (hTF) has one Fe(3+) ion bound by an aspartic acid, a histidine, two tyrosine residues, and two oxygens from the synergistic anion, carbonate. Extensive characterization of these ligands in the N-terminal lobe has been carried out. Despite sharing the same set of ligands...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050015f

    authors： Mason AB,Halbrooks PJ,James NG,Connolly SA,Larouche JR,Smith VC,MacGillivray RT,Chasteen ND

    更新日期：2005-06-07 00:00:00

  • Structural and thermodynamic characterization of the Escherichia coli RelBE toxin-antitoxin system: indication for a functional role of differential stability.

    abstract：:The RelE and RelB proteins constitute the RNA interferase (toxin) and its cognate inhibitor (antitoxin) components of the Escherichia coli relBE toxin-antitoxin system. Despite the well-described functionality and physiological activity of this system in E. coli, no structural study was performed on the folding and st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701037e

    authors： Cherny I,Overgaard M,Borch J,Bram Y,Gerdes K,Gazit E

    更新日期：2007-10-30 00:00:00

  • DNA cross-linking by intermediates in the mitomycin activation cascade.

    abstract：:We have assayed the cross-linking of oligonucleotides containing repeated mitomycin-reactive CpG sites in order to assess the factors that enhance activation of the carbamoyl function at C10, yielding efficient mitomycin cross-linking. Drugs studied include mitomycin C (MC), N-methylmitomycin A (NMA), and the aziridin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a049

    authors： Cera C,Egbertson M,Teng SP,Crothers DM,Danishefsky SJ

    更新日期：1989-06-27 00:00:00

  • Relationship between folding and function in a sequence-specific miniature DNA-binding protein.

    abstract：:Previously, we have described a miniature protein-based approach to the design of molecules that bind DNA or protein surfaces with high affinity and specificity. In this approach, the small, well-folded protein avian pancreatic polypeptide acts as a scaffold to present and stabilize an alpha-helical or PPII-helical re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050121h

    authors： Yang L,Schepartz A

    更新日期：2005-05-24 00:00:00

  • Interactions of hirudin-based inhibitor with thrombin: critical role of the IleH59 side chain of the inhibitor.

    abstract：:Hirudin is the most potent and specific thrombin inhibitor from medicinal leech with a Ki value of 2.2 x 10(-14) M. It consists of an active site inhibitor segment, hirudin1-48, a fibrinogen-recognition exosite inhibitor segment, hirudin55-65, and a linker, hirudin49-54, connecting these inhibitor segments. The role o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00027a021

    authors： Tsuda Y,Szewczuk Z,Wang J,Yue SY,Purisima E,Konishi Y

    更新日期：1995-07-11 00:00:00

  • Remodeling hexose-1-phosphate uridylyltransferase: mechanism-inspired mutation into a new enzyme, UDP-hexose synthase.

    abstract：:Hexose-1-phosphate uridylyltransferase catalyzes the interconversion of UDP-galactose and glucose-1-P with UDP-glucose and galactose-1-P by a double-displacement mechanism through a covalent intermediate (E-UMP), in which UMP is bonded to one of two histidine residues at the active site, H164 or H166. To identify whic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00499a003

    authors： Kim J,Ruzicka F,Frey PA

    更新日期：1990-11-27 00:00:00

  • Effect of flap modifications on human FEN1 cleavage.

    abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991321u

    authors： Bornarth CJ,Ranalli TA,Henricksen LA,Wahl AF,Bambara RA

    更新日期：1999-10-05 00:00:00

  • Redundancy of mammalian proteasome beta subunit function during endoplasmic reticulum associated degradation.

    abstract：:Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011322y

    authors： Oberdorf J,Carlson EJ,Skach WR

    更新日期：2001-11-06 00:00:00

  • Structures of normal single-stranded DNA and deoxyribo-3'-S-phosphorothiolates bound to the 3'-5' exonucleolytic active site of DNA polymerase I from Escherichia coli.

    abstract：:The interaction of a divalent metal ion with a leaving 3' oxygen is a central component of several proposed mechanisms of phosphoryl transfer. In support of this are recent kinetic studies showing that thiophilic metal ions (e.g., Mn2+) stimulate the hydrolysis of compounds in which sulfur takes the place of the leavi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981537g

    authors： Brautigam CA,Sun S,Piccirilli JA,Steitz TA

    更新日期：1999-01-12 00:00:00

  • Analysis of hydrogen bonding in enzyme-substrate complexes of chloramphenicol acetyltransferase by infrared spectroscopy and site-directed mutagenesis.

    abstract：:Chloramphenicol acetyltransferase (CAT) reversibly transfers an acetyl group between CoA and the 3-hydroxyl of either chloramphenicol (Cm) or 1-acetylchloramphenicol (1AcCm). The products of the forward reactions, 3-acetylchloramphenicol (3-AcCm) and 1,3-diacetylchloramphenicol (1,3Ac2-Cm), are the substrates for the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a003

    authors： Murray IA,Derrick JP,White AJ,Drabble K,Wharton CW,Shaw WV

    更新日期：1994-08-23 00:00:00

  • C5 cytosine methylation at CpG sites enhances sequence selectivity of mitomycin C-DNA bonding.

    abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991307h

    authors： Li VS,Reed M,Zheng Y,Kohn H,Tang M

    更新日期：2000-03-14 00:00:00

  • Two Mutations Commonly Associated with Daptomycin Resistance in Enterococcus faecium LiaST120A and LiaRW73C Appear To Function Epistatically in LiaFSR Signaling.

    abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01072

    authors： Davlieva M,Wu C,Zhou Y,Arias CA,Shamoo Y

    更新日期：2018-12-11 00:00:00

  • Expression and purification of the extracellular ligand-binding domain of the atrial natriuretic peptide (ANP) receptor: monovalent binding with ANP induces 2:2 complexes.

    abstract：:The receptor for atrial natriuretic peptide (ANP) is a type-I transmembrane protein containing an extracellular ligand-binding domain, a single transmembrane sequence, an intracellular kinase-homologous domain, and a guanylate cyclase (GCase) domain. Binding of ANP to the extracellular domain causes activation of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982127v

    authors： Misono KS,Sivasubramanian N,Berkner K,Zhang X

    更新日期：1999-01-12 00:00:00

  • Is Arg418 the catalytic base required for the hydrolysis step of the IMP dehydrogenase reaction?

    abstract：:The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048342v

    authors： Guillén Schlippe YV,Hedstrom L

    更新日期：2005-09-06 00:00:00

  • Cyanide binding at the non-heme Fe2+ of the iron-quinone complex of photosystem II: at high concentrations, cyanide converts the Fe2+ from high (S = 2) to low (S = 0) spin.

    abstract：:The primary electron acceptor complex of photosystem II, QAFe2+, can bind a number of small molecules at the iron site, including cyanide [Koulougliotis, D., Kostopoulos, T., Petrouleas, V., & Diner, B. A. (1993) Biochim. Biophys. Acta 1141, 275-282)]. In the presence of NaCN (30-300 mM) at pH 6.5, the reduced state, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a014

    authors： Sanakis Y,Petrouleas V,Diner BA

    更新日期：1994-08-23 00:00:00

  • Structural changes in cytochrome c upon hydrogen-deuterium exchange.

    abstract：:The resonance Raman spectra of yeast ferri- and ferro-iso-1-cytochrome c dissolved in H2O and D2O are reported. Hydrogen exchange in the protein leads to distinct spectral changes of heme vibrational bands, particularly in the region between 670 and 710 cm-1 and at approximately 443 and approximately 450 cm-1. The lat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00214a013

    authors： Hildebrandt P,Vanhecke F,Heibel G,Mauk AG

    更新日期：1993-12-28 00:00:00

  • A β-Turn Motif in the Steroid Hormone Receptor's Ligand-Binding Domains Interacts with the Peptidyl-prolyl Isomerase (PPIase) Catalytic Site of the Immunophilin FKBP52.

    abstract：:The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor α (hERα) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II β-turn conformat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00506

    authors： Byrne C,Henen MA,Belnou M,Cantrelle FX,Kamah A,Qi H,Giustiniani J,Chambraud B,Baulieu EE,Lippens G,Landrieu I,Jacquot Y

    更新日期：2016-09-27 00:00:00

  • E6*, the 50 amino acid product of the most abundant spliced transcript of the e6 oncoprotein in high-risk human papillomavirus, is a promiscuous folder and binder.

    abstract：:High-risk human papillomavirus E6 participates in tumorigenic progression, mainly by its ability to promote p53 degradation. HPV transcripts show a complex splicing pattern, where E6* is the most abundant transcript in high-risk HPV types, comprising the first 50 amino acids of E6. No structural or biochemical informa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101941c

    authors： Heer A,Alonso LG,de Prat-Gay G

    更新日期：2011-03-01 00:00:00

  • Steady-state and stopped-flow kinetic measurements of the primary deuterium isotope effect in the reaction catalyzed by p-cresol methylhydroxylase.

    abstract：:Steady-state kinetic studies for the reaction of the flavocytochrome p-cresol methylhydroxylase with the reducing substrates (S) p-cresol, 4-ethylphenol, and their corresponding alpha-deuteriated analogues are presented. The results from these experiments and those from studies involving various reoxidizing substrates...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00387a055

    authors： McIntire WS,Hopper DJ,Singer TP

    更新日期：1987-06-30 00:00:00

  • Intersubunit communication in tryptophan synthase by carbon-13 and fluorine-19 REDOR NMR.

    abstract：:The beta subunits of the 143-kDa alpha2beta2 tetrameric enzyme tryptophan synthase have been labeled by L-[ring-4-19F]phenylalanine and L-[phenol-4-13C]tyrosine in an effort to monitor the positions of these residues on ligand binding. Of the 13 phenylalanine and 11 tyrosine residues in the beta subunit, only three pa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9518297

    authors： McDowell LM,Lee M,McKay RA,Anderson KS,Schaefer J

    更新日期：1996-03-12 00:00:00

  • Membrane insertion and dissociation processes of a model transmembrane helix.

    abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026191v

    authors： Yano Y,Matsuzaki K

    更新日期：2002-10-15 00:00:00

  • Role of arginine-292 in the substrate specificity of aspartate aminotransferase as examined by site-directed mutagenesis.

    abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00412a052

    authors： Cronin CN,Kirsch JF

    更新日期：1988-06-14 00:00:00

  • The C-terminal lipid-binding domain of apolipoprotein E is a highly efficient mediator of ABCA1-dependent cholesterol efflux that promotes the assembly of high-density lipoproteins.

    abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602407r

    authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

    更新日期：2007-03-13 00:00:00

  • Structural and functional determinants of human plasma phospholipid transfer protein activity as revealed by site-directed mutagenesis of charged amino acids.

    abstract：:Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi027006g

    authors： Ponsin G,Qu SJ,Fan HZ,Pownall HJ

    更新日期：2003-04-22 00:00:00

  • Fluidity of sarcoplasmic reticulum membranes investigated with dipyrenylpropane, an intramolecular excimer probe.

    abstract：:Intramolecular excimer formation with 1,3-di(1-pyrenyl)propane was used to probe the fluidity of sarcoplasmic reticulum (SR) membranes where the probe could be incorporated (in a probe/lipid molar ratio of 1/2000 or less) without inducing any detectable damage. The temperature was varied between -1 and 40 degrees C. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00266a038

    authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

    更新日期：1982-11-09 00:00:00

  • Molecular interaction of DNA with bisplatinum(II) complexes having bis(vicinal 1,2-diamines) as ligand.

    abstract：:The interaction of novel, tetrafunctional bisplatinum compounds with DNA was investigated. These compounds have bis(vicinal 1,2-diamines) as ligand. The reactions' efficiency, types of cross-links, alterations of the global DNA structure, and sequence selectivity differ significantly from the corresponding features of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970741l

    authors： Büning H,Altman J,Beck W,Zorbas H

    更新日期：1997-09-23 00:00:00