当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Redundancy of mammalian proteasome beta subunit function during endoplasmic reticulum associated degradation.

Redundancy of mammalian proteasome beta subunit function during endoplasmic reticulum associated degradation.

Abstract：

:Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectively. Little is known about the relative contribution of specific beta subunits in the degradation of endogenous protein substrates. Using active site proteasome inhibitors and a reconstituted degradation system, we now show that all three active beta subunits can independently contribute to ER-associated degradation of the cystic fibrosis transmembrane conductance regulator (CFTR). Complete inactivation (>99.5%) of the beta5/X subunit decreased the rate of ATP-dependent conversion of CFTR to trichloroacetic acid soluble fragments by only 40%. Similarly, proteasomes containing only active beta1/Y or beta2/Z subunits degraded CFTR at approximately 50% of the rate observed for fully functional proteasomes. Simultaneous inhibition (>93%) of all three beta subunits blocked CFTR degradation by approximately 90%, and inhibition of both protease and ATPase activities was required to completely prevent generation of small peptide fragments. Our results demonstrate both a conserved hierarchy (ChT-L > PGPH > or = T-L) as well as a redundancy of beta subunit function and provide insight into the mechanism by which active site proteasome inhibitors influence degradation of endogenous protein substrates at the ER membrane.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Oberdorf J,Carlson EJ,Skach WR

doi

10.1021/bi011322y

subject

Has Abstract

pub_date

2001-11-06 00:00:00

pages

13397-405

issue

44

eissn

0006-2960

issn

1520-4995

pii

bi011322y

journal_volume

40

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • NMR studies of the phosphotransfer domain of the histidine kinase CheA from Escherichia coli: assignments, secondary structure, general fold, and backbone dynamics.

    abstract：:Multidimensional heteronuclear NMR techniques were applied to study the phosphotransfer domain, residues 1-134, of the histidine kinase CheA, from Escherichia coli, which contains the site of autophosphorylation, His48. Assignments of the backbone amide groups and side chain protons are nearly complete. Our studies sh...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00042a018

    authors： Zhou H,Lowry DF,Swanson RV,Simon MI,Dahlquist FW

    更新日期：1995-10-24 00:00:00

  • Channeling in sulfate activating complexes.

    abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060421e

    authors： Sun M,Leyh TS

    更新日期：2006-09-26 00:00:00

  • Quantitation of lateral stress in lipid layer containing nonbilayer phase preferring lipids by frequency-domain fluorescence spectroscopy.

    abstract：:Frequency-resolved fluorescence measurements have been performed to quantitate the lateral stress of the lipid layer containing nonbilayer phase preferring dioleoylphosphatidylethanolamine (DOPE). On the basis of a new rotational diffusion model, the wobbling diffusion constant (Dw), the curvature-related hopping diff...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00130a005

    authors： Chen SY,Cheng KH,Van der Meer BW

    更新日期：1992-04-21 00:00:00

  • Time-resolved fluorescence studies of genetically engineered Escherichia coli glutamine synthetase. Effects of ATP on the tryptophan-57 loop.

    abstract：:Single-tryptophan-containing mutants of low adenylation state Escherichia coli glutamine synthetase (wild type has two tryptophans at positions 57 and 158) have been constructed and studied by multifrequency phase/modulation fluorescence spectroscopy. The W57L mutant (retains tryptophan at residue 158) and the W158S m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00228a008

    authors： Atkins WM,Stayton PS,Villafranca JJ

    更新日期：1991-04-09 00:00:00

  • Effect of the carbohydrate moiety on the secondary structure of beta 2-glycoprotein. I. Implications for the biosynthesis and folding of glycoproteins.

    abstract：:By use of six highly purified exoglycosidases with well-defined specificity, the oligosaccharide units of human plasma beta 2-glycoprotein I (beta 2I) were modified by sequential enzymatic degradation. The released monosaccharides (NeuAc, Gal, GlcNAc, and Man) were quantified, and the carbohydrate compositions of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00478a020

    authors： Walsh MT,Watzlawick H,Putnam FW,Schmid K,Brossmer R

    更新日期：1990-07-03 00:00:00

  • Laser temperature jump study of solvent effects of poly(adenylic acid) stacking.

    abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00549a025

    authors： Dewey TG,Turner DH

    更新日期：1980-04-15 00:00:00

  • NMR solution structure of the N3' --> P5' phosphoramidate duplex d(CGCGAATTCGCG)2 by the iterative relaxation matrix approach.

    abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980711y

    authors： Ding D,Gryaznov SM,Wilson WD

    更新日期：1998-09-01 00:00:00

  • Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa.

    abstract：:The enzyme RhlI catalyzes the formation of N-butyrylhomoserine lactone from S-adenosylmethionine and N-butyrylacyl carrier protein. N-Butyrylhomoserine lactone serves as a quorum-sensing signal molecule in Pseudomonas aeruginosa, and is implicated in the regulation of many processes involved in bacterial virulence and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048005m

    authors： Raychaudhuri A,Jerga A,Tipton PA

    更新日期：2005-03-01 00:00:00

  • Carbohydrate chains of human thyrotropin are differentially susceptible to endoglycosidase removal on combined and free polypeptide subunits.

    abstract：:The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00392a040

    authors： Ronin C,Papandreou MJ,Canonne C,Weintraub BD

    更新日期：1987-09-08 00:00:00

  • Identification of a region of the ileal-type sodium/bile acid cotransporter interacting with a competitive bile acid transport inhibitor.

    abstract：:Drug intervention that prevents reabsorption of circulating bile acids by the apical (ileal) sodium/bile acid cotransporter (ASBT) may be a promising new therapy for lowering of plasma cholesterol. 2164U90 is a benzothiazepine-based competitive inhibitor of bile acid transport with K(i) values of approximately 10 and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0205404

    authors： Hallén S,Björquist A,Ostlund-Lindqvist AM,Sachs G

    更新日期：2002-12-17 00:00:00

  • Zeitlupe senses blue-light fluence to mediate circadian timing in Arabidopsis thaliana.

    abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401027n

    authors： Pudasaini A,Zoltowski BD

    更新日期：2013-10-08 00:00:00

  • Structure of the cytoplasmic loop between putative helices II and III of the mannitol permease of Escherichia coli: a tryptophan and 5-fluorotryptophan spectroscopy study.

    abstract：:In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8020668

    authors： Vos EP,Bokhove M,Hesp BH,Broos J

    更新日期：2009-06-16 00:00:00

  • Solution structure of the lipophosphoglycan of Leishmania donovani.

    abstract：:The three-dimensional solution structure of the repeating -PO4-6Gal beta 1-4Man alpha 1- disaccharide fragment of the lipophosphoglycan (LPG) derived from Leishmania donovani has been determined by use of a combination of homo- and heteronuclear NMR spin coupling constant measurements together with restrained molecula...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00118a004

    authors： Homans SW,Mehlert A,Turco SJ

    更新日期：1992-01-28 00:00:00

  • NMR backbone dynamics of the human type I interferon binding subunit, a representative cytokine receptor.

    abstract：:The antiviral and antiproliferative activities of type I interferons (IFNs) are mediated by a common receptor, and its second subunit (IFNAR2) exhibits nanomolar affinity to both IFNalpha and IFNbeta subtypes. We have previously determined the structure of the IFN-binding extracellular domain of IFNAR2 (IFNAR2-EC) usi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049606g

    authors： Chill JH,Quadt SR,Anglister J

    更新日期：2004-08-10 00:00:00

  • Orientation factor in steady-state and time-resolved resonance energy transfer measurements.

    abstract：:Resonance energy transfer measurements provide a way to estimate distances between chromophores attached to different sites of macromolecules. There are two unknowns involved in resonance energy transfer measurements, the distance between two chromophores and their relative orientation. When static orientational disor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00149a027

    authors： Wu P,Brand L

    更新日期：1992-09-01 00:00:00

  • Metal ion catalysis of RNA cleavage by the influenza virus endonuclease.

    abstract：:The influenza virus RNA-dependent RNA polymerase protein complex contains an associated RNA endonuclease activity, which cleaves host mRNA precursors in the cell nucleus at defined positions 9-15 nucleotides downstream of the cap structure. This reaction provides capped oligoribonucleotides, which function as primers ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9828932

    authors： Doan L,Handa B,Roberts NA,Klumpp K

    更新日期：1999-04-27 00:00:00

  • The C-terminal lipid-binding domain of apolipoprotein E is a highly efficient mediator of ABCA1-dependent cholesterol efflux that promotes the assembly of high-density lipoproteins.

    abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602407r

    authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

    更新日期：2007-03-13 00:00:00

  • Polypeptide clearing in model membranes: an analysis of the partition of gramicidin a' between cadmium ion induced gel and liquid-crystalline phases in vesicles of phosphatidic acid and phosphatidylcholine.

    abstract：:Using a simple model for a biological membrane we examine cation-induced gel phase formation and the depletion of polypeptide from the gel phase. The model system consists of vesicles of phosphatidic acid and phosphatidylcholine which contain gramicidin A'. By use of electron spin resonance to monitor lipid phase beha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00282a013

    authors： Feigenson GW

    更新日期：1983-06-21 00:00:00

  • Fusion of phosphatidylcholine bilayer vesicles: role of free fatty acid.

    abstract：:The transformation of DMPC vesicles containing myristic acid to larger structures was examined by nuclear magnetic resonance techniques. The process was found to be characterized by a rate which is approximately first order in vesicle concentration. Rates, however, show a dramatic increase with increasing myristic aci...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00610a027

    authors： Kantor HL,Prestegard JH

    更新日期：1978-08-22 00:00:00

  • Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration.

    abstract：:Nitric oxide (NO) regulates cardiovascular hemostasis by binding to soluble guanylyl cyclase (sGC), leading to cGMP production, reduced cytosolic calcium concentration ([Ca(2+)](i)), and vasorelaxation. Thrombospondin-1 (TSP-1), a secreted matricellular protein, was recently discovered to inhibit NO signaling and sGC ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201060c

    authors： Ramanathan S,Mazzalupo S,Boitano S,Montfort WR

    更新日期：2011-09-13 00:00:00

  • N-Terminal Region of Vibrio parahemolyticus Thermostable Direct Hemolysin Regulates the Membrane-Damaging Action of the Toxin.

    abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00937

    authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

    更新日期：2020-02-04 00:00:00

  • Purification and characterization of the messenger ribonucleic acid capping enzyme GTP:RNA guanylyltransferase from wheat germ.

    abstract：:A GTP:RNA guanylyltransferase or capping enzyme has been purified approximately 2000-fold from wheat germ. The enzyme catalyzes the transfer of the GMP residue from GTP to the 5' end of RNA or synthetic polyribonucleotides. Diphosphate-ended polymers were capped more efficiently than molecules with triphosphate ends, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00531a020

    authors： Keith JM,Venkatesan S,Gershowitz A,Moss B

    更新日期：1982-01-19 00:00:00

  • Backbone dynamics of the glucocorticoid receptor DNA-binding domain.

    abstract：:The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00163a007

    authors： Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

    更新日期：1992-12-08 00:00:00

  • Antisense L/D-oligodeoxynucleotide chimeras: nuclease stability, base-pairing properties, and activity at directing ribonuclease H.

    abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00191a015

    authors： Damha MJ,Giannaris PA,Marfey P

    更新日期：1994-06-28 00:00:00

  • Correlation between the inhibition of the acto-heavy meromyosin ATPase and the binding of tropomyosin to F-actin: effects of Mg2+, KCl, troponin I, and troponin C.

    abstract：:When stoichiometric amounts of tropomyosin (TM) are bound to F-actin in the presence of 2 mM ATP, the MG2+-activated acto-heavy meromyosin (HMM) ATPase is inhibited by about 60% in 5 mM MgCl2-30 mM KCl. If the concentration of MgCl2 is reduced to 1 mM, the inhibition disappears because TM no longer binds to F-actin. I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00683a025

    authors： Eaton BL,Kominz DR,Eisenberg E

    更新日期：1975-06-17 00:00:00

  • Biosynthesis of lipid A in Escherichia coli: identification of UDP-3-O-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine as a precursor of UDP-N2,O3-bis[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine.

    abstract：:The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00406a017

    authors： Anderson MS,Robertson AD,Macher I,Raetz CR

    更新日期：1988-03-22 00:00:00

  • Structural coupling between the oxygen-evolving Mn cluster and a tyrosine residue in photosystem II as revealed by Fourier transform infrared spectroscopy.

    abstract：:The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971760y

    authors： Noguchi T,Inoue Y,Tang XS

    更新日期：1997-12-02 00:00:00

  • Role of glycosylation on the secretion and biological activity of erythropoietin.

    abstract：:The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00156a003

    authors： Delorme E,Lorenzini T,Giffin J,Martin F,Jacobsen F,Boone T,Elliott S

    更新日期：1992-10-20 00:00:00

  • Phenothiazine-binding and attachment sites of CAPP1-calmodulin.

    abstract：:In the presence of Ca2+ norchlorpromazine isothiocyanate forms a monocovalent complex with calmodulin: CAPP1-calmodulin (Newton et al, 1983). Trypsin digestion of [3H]CAPP1-calmodulin yields as the major radioactive peptide N epsilon-CAPP-Lys-Met-Lys, corresponding to residues 75-77 of calmodulin. Stoichiometric amoun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a019

    authors： Newton DL,Klee CB

    更新日期：1989-05-02 00:00:00

  • Dependence of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen photoaddition on the conformation of ribonucleic acid.

    abstract：:The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at differe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00535a040

    authors： Thompson JF,Bachellerie JP,Hall K,Hearst JE

    更新日期：1982-03-16 00:00:00