当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > A β-Turn Motif in the Steroid Hormone Receptor's Ligand-Binding Domains Interacts with the Peptidyl-prolyl Isomerase (PPIase) Catalytic Site of the Immunophilin FKBP52.

A β-Turn Motif in the Steroid Hormone Receptor's Ligand-Binding Domains Interacts with the Peptidyl-prolyl Isomerase (PPIase) Catalytic Site of the Immunophilin FKBP52.

Abstract：

:The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor α (hERα) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II β-turn conformation in the protein, binds the peptidyl-prolyl isomerase (PPIase or rotamase) FK1 domain of FKBP52. Interestingly, this turn motif displays strong similarities with the FKBP52 FK1 domain-binding moiety of macrolide immunomodulators such as rapamycin and GPI-1046, an immunophilin ligand with neuroprotective characteristics. An increase in the hydrophobicity of the residue preceding the proline and cyclization of the VPGF peptide strengthen its recognition by the FK1 domain of FKBP52. Replacement of the Pro residue with a dimethylproline also enhances this interaction. Our study not only contributes to a better understanding of how the interaction between the FK1 domain of FKBP52 and steroid hormone receptors most likely works but also opens new avenues for the synthesis of FKBP52 FK1 peptide ligands appropriate for the control of hormone-dependent physiological mechanisms or of the functioning of the Tau protein. Indeed, it has been shown that FKBP52 is involved in the intraneuronal dynamics of the Tau protein.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Byrne C,Henen MA,Belnou M,Cantrelle FX,Kamah A,Qi H,Giustiniani J,Chambraud B,Baulieu EE,Lippens G,Landrieu I,Jacquot Y

doi

10.1021/acs.biochem.6b00506

subject

Has Abstract

pub_date

2016-09-27 00:00:00

pages

5366-76

issue

38

eissn

0006-2960

issn

1520-4995

journal_volume

55

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Entrapment of Water at the Transmembrane Helix-Helix Interface of Quiescin Sulfhydryl Oxidase 2.

    abstract：:Little is known about how a membrane can regulate interactions between transmembrane helices. Here, we show that strong self-interaction of the transmembrane helix of human quiescin sulfhydryl oxidase 2 rests on a motif of conserved amino acids comprising one face of the helix. Atomistic molecular dynamics simulations...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01239

    authors： Ried CL,Scharnagl C,Langosch D

    更新日期：2016-03-08 00:00:00

  • r-VKORC1 expression in factor IX BHK cells increases the extent of factor IX carboxylation but is limited by saturation of another carboxylation component or by a shift in the rate-limiting step.

    abstract：:Carboxylation of vitamin K-dependent (VKD) proteins is required for their activity and depends on reduced vitamin K generated by vitamin K oxidoreductase (VKOR) and a redox protein that regenerates VKOR activity. VKD protein carboxylation is inefficient in mammalian cells, and to understand why carboxylation becomes s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051986y

    authors： Hallgren KW,Qian W,Yakubenko AV,Runge KW,Berkner KL

    更新日期：2006-05-02 00:00:00

  • The newly discovered Parkinson's disease associated Finnish mutation (A53E) attenuates α-synuclein aggregation and membrane binding.

    abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5010365

    authors： Ghosh D,Sahay S,Ranjan P,Salot S,Mohite GM,Singh PK,Dwivedi S,Carvalho E,Banerjee R,Kumar A,Maji SK

    更新日期：2014-10-21 00:00:00

  • Structural and functional analysis of peptidyl oligosaccharyl transferase inhibitors.

    abstract：:The peptide cyclo(hex-Amb(1)-Cys(2))-Thr(3)-Val(4)-Thr(5)-Nph(6)-NH2 was previously shown to be a slow, tight-binding inhibitor (Ki = 37 nM) of the yeast oligosaccharyl transferase (OT) [Hendrickson et al. (1996) J. Am. Chem. Soc. 118, 7636-7637]. This enzyme catalyzes the transfer of a carbohydrate moiety to an aspar...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971465d

    authors： Kellenberger C,Hendrickson TL,Imperiali B

    更新日期：1997-10-14 00:00:00

  • Understanding the Species Selectivity of Myeloid Cell Leukemia-1 (Mcl-1) Inhibitors.

    abstract：:To test for on target toxicity of a new chemical entity, it is important to have comparable binding affinities of the compound in the target proteins from humans and the test species. To evaluate our myeloid cell leukemia-1 (Mcl-1) inhibitors, we tested them against rodent Mcl-1 and found a significant loss of binding...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00626

    authors： Zhao B,Arnold AL,Coronel MA,Lee JH,Lee T,Olejniczak ET,Fesik SW

    更新日期：2018-08-14 00:00:00

  • The binding of complementary oligoribonucleotides to yeast initiator Transfer RNA.

    abstract：:Oligoribonucleotide binding to baker's yeast initiator tRNA was measured by equilibrium dialysis in order to determine which regions of the tRNA were free to bind complementary oligomers and which were involved in secondary and tertiary structure. Association constants of trinucleoside diphosphates and tetranucleoside...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00686a004

    authors： Freier SM,Tinoco I Jr

    更新日期：1975-07-29 00:00:00

  • A study of protein-sodium dodecyl sulfate complexes by transient electric birefringence.

    abstract：:The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00685a030

    authors： Wright AK,Thompson MR,Miller RL

    更新日期：1975-07-15 00:00:00

  • Thermodynamic and structural properties of the acid molten globule state of horse cytochrome C.

    abstract：:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101806b

    authors： Nakamura S,Seki Y,Katoh E,Kidokoro S

    更新日期：2011-04-19 00:00:00

  • Four-fold channels are involved in iron diffusion into the inner cavity of plant ferritin.

    abstract：:From an evolutionary point of view, plant and animal ferritins arose from a common ancestor, but plant ferritin exhibits different features as compared with the animal analogue. One major difference is that the 4-fold channels naturally occurring in plant ferritin are hydrophilic, whereas the 4-fold channels in animal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500066m

    authors： Lv C,Zhang S,Zang J,Zhao G,Xu C

    更新日期：2014-04-15 00:00:00

  • Structure of the bovine elastin gene and S1 nuclease analysis of alternative splicing of elastin mRNA in the bovine nuchal ligament.

    abstract：:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00432a003

    authors： Yeh H,Anderson N,Ornstein-Goldstein N,Bashir MM,Rosenbloom JC,Abrams W,Indik Z,Yoon K,Parks W,Mecham R

    更新日期：1989-03-21 00:00:00

  • Receptor site for the 5'-phosphate of elongator tRNAs governs substrate selection by peptidyl-tRNA hydrolase.

    abstract：:Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982657r

    authors： Fromant M,Plateau P,Schmitt E,Mechulam Y,Blanquet S

    更新日期：1999-04-20 00:00:00

  • Ca2+-signaling cycle of a membrane-docking C2 domain.

    abstract：:The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9717340

    authors： Nalefski EA,Slazas MM,Falke JJ

    更新日期：1997-10-07 00:00:00

  • Binding of adenosine diphosphoribosyltransferase to the termini and internal regions of linear DNAs.

    abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a050

    authors： Sastry SS,Buki KG,Kun E

    更新日期：1989-06-27 00:00:00

  • Role of tryptophanyl and tyrosyl residues of flavoproteins in binding with flavin coenzymes. X-ray structural studies using model complexes.

    abstract：:The crystal structures of 7,8-dimethylisoalloxazine-10-acetic acid-tryptamine (1:1) tetrahydrate and 7,8-dimethylisoalloxazine-10-acetic acid-tyramine (1:1) tetrahydrate complexes were determined by the X-ray diffraction method, as models for flavin-tryptophan and flavin-tyrosine interactions in flavoproteins. The obs...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00513a034

    authors： Inoue M,Shibata M,Kondo Y,Ishida T

    更新日期：1981-05-12 00:00:00

  • Interactions of cationic ligands and proteins with small nucleic acids: analytic treatment of the large coulombic end effect on binding free energy as a function of salt concentration.

    abstract：:For nonspecific binding of oligopeptides and other cationic ligands, including proteins, to nucleic acid oligomers, we develop a model capable of quantifying and predicting the salt concentration dependence of the binding free energy (deltaG(o)obs) by way of an analytic treatment of the Coulombic end effect (CEE). Lig...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0520434

    authors： Shkel IA,Ballin JD,Record MT Jr

    更新日期：2006-07-11 00:00:00

  • Perturbation of the carboxy terminus of HIV-1 Rev affects multimerization on the Rev responsive element.

    abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00085a028

    authors： Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

    更新日期：1993-08-31 00:00:00

  • Proton involvement in the transition from the "peroxy" to the ferryl intermediate of cytochrome c oxidase.

    abstract：:In the absence of any external electron donor, the "peroxy" intermediate of cytochrome c oxidase (CcO-607) is converted to the ferryl form (CcO-580) and subsequently to oxidized enzyme. The rate of conversion of CcO-607 to the CcO-580 form is pH dependent between pH 3.0 and pH 7.6. A plot of the logarithm of the rate ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002373i

    authors： Fabian M,Palmer G

    更新日期：2001-02-13 00:00:00

  • Amino Acid Selective 13C Labeling and 13C Scrambling Profile Analysis of Protein α and Side-Chain Carbons in Escherichia coli Utilized for Protein Nuclear Magnetic Resonance.

    abstract：:Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00182

    authors： Sugiki T,Furuita K,Fujiwara T,Kojima C

    更新日期：2018-07-03 00:00:00

  • Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

    abstract：:We report a straightforward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity and inhibition of fucosyltransferase VIII, which catal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00286

    authors： Soroko M,Kwan DH

    更新日期：2020-06-09 00:00:00

  • Probes of hydrogen tunneling with horse liver alcohol dehydrogenase at subzero temperatures.

    abstract：:The temperature dependence of steady-state kinetics has been studied with horse liver alcohol dehydrogenase (HLADH) using protonated and deuterated benzyl alcohol as substrates in methanol/water mixtures between +3 and -50 degrees C. Additionally, the competitive isotope effects, k(H)/k(T) and k(D)/k(T), were measured...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002075l

    authors： Tsai S,Klinman JP

    更新日期：2001-02-20 00:00:00

  • Iron regulatory element and internal loop/bulge structure for ferritin mRNA studied by cobalt(III) hexammine binding, molecular modeling, and NMR spectroscopy.

    abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9719814

    authors： Gdaniec Z,Sierzputowska-Gracz H,Theil EC

    更新日期：1998-02-10 00:00:00

  • Probing adenine rings and backbone linkages using base specific isotope-edited Raman spectroscopy: application to group II intron ribozyme domain V.

    abstract：:Raman difference spectroscopy is used to probe the properties of a 36-nt RNA molecule, "D5", which lies at the heart of the catalytic apparatus in group II introns. For D5 that has all of its adenine residues labeled with (13)C and (15)N and utilizing Raman difference spectroscopy, we identify the conformationally sen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902117w

    authors： Chen Y,Eldho NV,Dayie TK,Carey PR

    更新日期：2010-04-27 00:00:00

  • Cembranoid and long-chain alkanol sites on the nicotinic acetylcholine receptor and their allosteric interaction.

    abstract：:Long-chain alkanols are general anesthetics which can also act as uncharged noncompetitive inhibitors of the peripheral nicotinic acetylcholine receptor (AChR) by binding to one or more specific sites on the AChR. Cembranoids are naturally occurring, uncharged noncompetitive inhibitors of peripheral and neuronal AChRs...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0112255

    authors： Pagán OR,Eterović VA,Garcia M,Vergne D,Basilio CM,Rodríguez AD,Hann RM

    更新日期：2001-09-18 00:00:00

  • Structural and functional comparisons of nucleotide pyrophosphatase/phosphodiesterase and alkaline phosphatase: implications for mechanism and evolution.

    abstract：:The rapid expansion of the amount of genomic and structural data has provided many examples of enzymes with evolutionarily related active sites that catalyze different reactions. Functional comparisons of these active sites can provide insight into the origins of the enormous catalytic proficiency of enzymes and the e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060847t

    authors： Zalatan JG,Fenn TD,Brunger AT,Herschlag D

    更新日期：2006-08-15 00:00:00

  • Fibrillar amyloid beta-protein binds protease nexin-2/amyloid beta-protein precursor: stimulation of its inhibition of coagulation factor XIa.

    abstract：:Cerebrovascular deposition of fibrillar 39-42 amino acid amyloid beta-protein (Abeta), a condition known as cerebral amyloid angiopathy (CAA), is a key pathological feature of Alzheimer's disease and related disorders including hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D). Severe cases of CAA, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0002840

    authors： Wagner MR,Keane DM,Melchor JP,Auspaker KR,Van Nostrand WE

    更新日期：2000-06-27 00:00:00

  • Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity.

    abstract：:We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101102u

    authors： Weeks AM,Coyle SM,Jinek M,Doudna JA,Chang MC

    更新日期：2010-11-02 00:00:00

  • Mutational analysis of 26 residues of vaccinia DNA topoisomerase identifies Ser-204 as important for DNA binding and cleavage.

    abstract：:Vaccinia DNA topoisomerase, a 314 amino acid type I enzyme, catalyzes the cleavage and rejoining of DNA strands through a DNA-(3'-phosphotyrosyl)-enzyme intermediate formed at a specific target sequence, 5'-(C/T)CCTT downward arrow. To identify amino acids that participate in the DNA binding and transesterification st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970498q

    authors： Wang LK,Wittschieben J,Shuman S

    更新日期：1997-07-01 00:00:00

  • Structural and functional analysis of the HIV gp41 core containing an Ile573 to Thr substitution: implications for membrane fusion.

    abstract：:The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0024759

    authors： Liu J,Shu W,Fagan MB,Nunberg JH,Lu M

    更新日期：2001-03-06 00:00:00

  • Site-specific variations in RNA folding thermodynamics visualized by 2-aminopurine fluorescence.

    abstract：:The fluorescent base analogue 2-aminopurine (2-AP) is commonly used to study specific conformational and protein binding events involving nucleic acids. Here, combinations of steady-state and time-resolved fluorescence spectroscopy of 2-AP were employed to monitor conformational transitions within a model hairpin RNA ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7011977

    authors： Ballin JD,Bharill S,Fialcowitz-White EJ,Gryczynski I,Gryczynski Z,Wilson GM

    更新日期：2007-12-11 00:00:00

  • Interaction of fusogenic synthetic peptide with phospholipid bilayers: orientation of the peptide alpha-helix and binding isotherm.

    abstract：:We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952547+

    authors： Ishiguro R,Matsumoto M,Takahashi S

    更新日期：1996-04-16 00:00:00