Ca2+-signaling cycle of a membrane-docking C2 domain.

abstract：:The beta-glucosidase from Agrobacterium sp. catalyzes the hydrolysis of beta-glucosides via a covalent alpha-D-glucopyranosyl-enzyme intermediate involving Glu358. Hydrolysis of 2,4-dinitrophenyl beta-D-glucopyranoside by the low activity Glu358Asp mutant of Agrobacterium beta-glucosidase is accompanied by time-depend...

journal_title：Biochemistry

authors： Gebler JC,Trimbur DE,Warren AJ,Aebersold R,Namchuk M,Withers SG

更新日期：1995-11-07 00:00:00

abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

journal_title：Biochemistry

authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

更新日期：2001-08-21 00:00:00

abstract：:Solvent proton nuclear magnetic dispersion studies at 25, 100, and 300 MHz have been performed on protocatechuate 3,4-dioxygenase (PCD) and its complexes with 3-chloro-4-hydroxybenzoate and 3-fluoro-4-hydroxybenzoate. Longitudinal and transverse relaxation rates were measured for these compounds and for the apoenzyme....

journal_title：Biochemistry

authors： Felton RH,Gordon SL,Sowell AL,May SW

更新日期：1984-08-14 00:00:00

abstract：:Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...

journal_title：Biochemistry

authors： Mitchell EP,Withers SG,Ermert P,Vasella AT,Garman EF,Oikonomakos NG,Johnson LN

更新日期：1996-06-11 00:00:00

abstract：:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial se...

journal_title：Biochemistry

authors： Oakley MG,Kim PS

更新日期：1997-03-04 00:00:00

abstract：:The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...

journal_title：Biochemistry

authors： Dunn SM,Raftery MA

更新日期：1993-08-24 00:00:00

abstract：:Despite the expression of only one type of receptor, there is great variation in the ability of different cell types to discriminate between C5a and its more stable metabolite, C5a des Arg74. The mechanism that underlies this phenomenon is not understood but presumably involves differences in the interaction with the ...

journal_title：Biochemistry

authors： Crass T,Bautsch W,Cain SA,Pease JE,Monk PN

更新日期：1999-07-27 00:00:00

abstract：:A panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal IgG. After exhaustive Pronase digestion of IgG, glycopeptides were characterized chromatographically by apparent size, charge, and concanavalin A (Con A)-Sepharose and Lens...

journal_title：Biochemistry

authors： Rothman RJ,Warren L,Vliegenthart JF,Härd KJ

更新日期：1989-02-07 00:00:00

abstract：:Fapy.dG is produced in DNA as a result of oxidative stress from a precursor that also forms OxodG. Bypass of Fapy.dG in a shuttle vector in COS-7 cells produces G --> T transversions slightly more frequently than does OxodG (Kalam, M. A., et al. (2006) Nucleic Acids Res. 34, 2305). The effect of Fapy.dG on replication...

journal_title：Biochemistry

authors： Patro JN,Wiederholt CJ,Jiang YL,Delaney JC,Essigmann JM,Greenberg MM

更新日期：2007-09-04 00:00:00

abstract：:The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...

journal_title：Biochemistry

authors： Farhangrazi ZS,Fossett ME,Powers LS,Ellis WR Jr

更新日期：1995-03-07 00:00:00

abstract：:p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...

journal_title：Biochemistry

authors： Koyama T,Obata S,Saito K,Takeshita-Koike A,Ogura K

更新日期：1994-10-25 00:00:00

abstract：:Limited digestion of polysomal RNA with pancreatic ribonuclease releases a structure consisting of poly(A) associated with other polyribonucleotide sequences. This complex can be purified by oligo(dT)-cellulose chromatography. Heating for formamide treatment causes the dissociation of fragments free of poly(A) from th...

journal_title：Biochemistry

authors： Jeffery WR,Brawerman G

更新日期：1975-07-29 00:00:00

abstract：:The phosphorylation of (Na+, K+)ATPase from the electric organ of the electric eel is dependent on Mg2+. The amount of phosphoenzyme formed was increased by K+ and decreased by Na+. Kinetic analyses indicate that a ternary complex of ATPase, Pi and Mg2+ is formed prior to phosphorylation of the protein. Calorimetric s...

journal_title：Biochemistry

authors： Kuriki Y,Halsey J,Biltonen R,Racker E

更新日期：1976-11-16 00:00:00

abstract：:The Fpg protein from Escherichia coli is a multifunctional protein that excises damaged purine bases from DNA to generate aldehydic abasic sites and then catalyzes the successive cleavage of the phosphodiester bonds first on the 3'-side and then on the 5'-side of the abasic site to generate 5'- and 3'-phosphate ends, ...

journal_title：Biochemistry

authors： Bhagwat M,Gerlt JA

更新日期：1996-01-16 00:00:00

abstract：:The enzyme 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (EC 4.1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was inactivated by the thiol-m...

journal_title：Biochemistry

authors： Salleh HM,Patel MA,Woodard RW

更新日期：1996-07-09 00:00:00

abstract：:The FMN prosthetic group of flavocytochrome b2 or L-lactate dehydrogenase oxidizes lactate to pyruvate. The reducing equivalents are then transferred one by one, intramolecularly, to heme b2 and then to external acceptors. Substrate oxidation is thought to begin with abstraction of the substrate alpha-hydrogen as a pr...

journal_title：Biochemistry

authors： Gondry M,Lederer F

更新日期：1996-07-02 00:00:00

abstract：:This report describes the determination of the complete primary structure of the adhesin receptor polysaccharide of Streptococcus oralis ATCC 55229 (previously characterized as Streptococcus sanguis H1), a Gram-positive bacteria implicated in dental plaque formation. The polysaccharide was isolated from S. oralis ATCC...

journal_title：Biochemistry

authors： Glushka J,Cassels FJ,Carlson RW,van Halbeek H

更新日期：1992-11-10 00:00:00

abstract：:Glu58 is known to participate in phosphodiester transesterification catalyzed by the enzyme RNase T1. For Glu58 RNase T1, an altered mechanism has been proposed in which His40 replaces Glu58 as the base catalyst [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-9072]. Glu58Ala Rnase T...

journal_title：Biochemistry

authors： Pletinckx J,Steyaert J,Zegers I,Choe HW,Heinemann U,Wyns L

更新日期：1994-02-22 00:00:00

abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...

journal_title：Biochemistry

authors： Sato M,Kanamori T,Kamo N,Demura M,Nitta K

更新日期：2002-02-19 00:00:00

abstract：:This study presents the first detailed examination by resonance Raman (RR) spectroscopy of the rates of solvent exchange for the C5 and C3 positions of the TPQ cofactor in several wild-type copper-containing amine oxidases and mutants of the amine oxidase from Hansenula polymorpha (HPAO). On the basis of crystal struc...

journal_title：Biochemistry

authors： Green EL,Nakamura N,Dooley DM,Klinman JP,Sanders-Loehr J

更新日期：2002-01-15 00:00:00

abstract：:Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

journal_title：Biochemistry

authors： Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

更新日期：2000-11-14 00:00:00

abstract：:We have employed molecular dynamics (MD) simulation to investigate, with atomic details, the structural dynamics and energetics of three major ATPase states (ADP, APO, and ATP state) of a human kinesin-1 monomer in complex with a tubulin dimer. Starting from a recently solved crystal structure of ATP-like kinesin-tubu...

journal_title：Biochemistry

authors： Chakraborty S,Zheng W

更新日期：2015-01-27 00:00:00

abstract：:A 31 amino acid fragment of the extracellular N-terminus of the human G-protein coupled receptor for parathyroid hormone (PTH1R) has been structurally characterized by NMR and molecular dynamics simulations. The fragment PTH1R[168-198] includes residues 173-189, shown by photoaffinity cross-linking to be a contact dom...

journal_title：Biochemistry

authors： Pellegrini M,Bisello A,Rosenblatt M,Chorev M,Mierke DF

更新日期：1998-09-15 00:00:00

abstract：:Phosphorylation of G protein-coupled receptors (GPCRs) terminates their ability to couple with and activate G proteins by increasing their affinity for arrestins. Unfortunately, detailed information regarding how GPCRs interact with the kinases responsible for their phosphorylation is still limited. Here, we purified ...

journal_title：Biochemistry

authors： Jones Brunette AM,Sinha A,David L,Farrens DL

更新日期：2016-06-07 00:00:00

abstract：:tRNase Z, which exists in almost all cells, is believed to be working primarily for tRNA 3' maturation. In Escherichia coli, however, the tRNase Z gene appears to be dispensable under normal growth conditions, and its physiological role is not clear. Here, to investigate a possibility that E. coli tRNase Z cleaves RNA...

journal_title：Biochemistry

authors： Shibata HS,Minagawa A,Takaku H,Takagi M,Nashimoto M

更新日期：2006-05-02 00:00:00

abstract：:The phosphorylation mechanism of Dictyostelium discoideum nucleoside diphosphate (NDP) kinase was investigated by NMR. 31P chemical shifts were measured on both native and denatured enzyme. In the enzymatically phosphorylated enzyme denatured by 9 M urea or 7 M guanidine hydrochloride, the NDP kinase phosphohistidine ...

journal_title：Biochemistry

authors： Lecroisey A,Lascu I,Bominaar A,Véron M,Delepierre M

更新日期：1995-09-26 00:00:00

abstract：:Hemocyanin (Hc) is a dinuclear copper protein that binds oxygen reversibly. The structure of the Cu(II) site in a derivative of hemocyanin known as green half-met (GHM) has been analyzed using the pulsed EPR technique of electron spin-echo envelope modulation (ESEEM) spectroscopy. The derivative, prepared by treating ...

journal_title：Biochemistry

authors： Magliozzo RS,Bubacco L,McCracken J,Jiang F,Beltramini M,Salvato B,Peisach J

更新日期：1995-02-07 00:00:00

abstract：:The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...

journal_title：Biochemistry

authors： Marche P,Montenay-Garestier T,Fromageot P,Hélène C

更新日期：1976-12-28 00:00:00

abstract：:The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...

journal_title：Biochemistry

authors： Ackermann JM,Kanugula S,Pegg AE

更新日期：2005-02-15 00:00:00

abstract：:The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 ami...

journal_title：Biochemistry

authors： Arndt JW,Chai Q,Christian T,Stevens RC

更新日期：2006-03-14 00:00:00