Abstract：
:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial seryl tRNA synthetases (SRS). A recombinant 82-residue peptide corresponding to the helical arm of Escherichia coli SRS folds into a stable, monomeric, helical structure in the absence of the rest of the protein, as shown by circular dichroism (CD) and equilibrium sedimentation centrifugation. However, peptides corresponding to the individual helices of SRS are unstructured at neutral pH and do not associate appreciably at total peptide concentrations up to 100 microM. Covalent attachment of the the two peptides through a nonnatural, disulfide-containing linker restores structure and allows study of variants in which the individual helices are constrained to interact in either an antiparallel or a parallel orientation. We find that the antiparallel species are substantially more helical and more stable to thermal denaturation than their parallel counterpart. Thus, the SRS helical arm is an autonomously folding unit, and, unlike most other coiled coils, has an intrinsic preference for an antiparallel orientation of its constituent helices.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Oakley MG,Kim PSdoi
10.1021/bi962391tsubject
Has Abstractpub_date
1997-03-04 00:00:00pages
2544-9issue
9eissn
0006-2960issn
1520-4995pii
bi962391tjournal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Because of the conflicting conclusions that have been reached regarding the location of the two putative membrane-spanning segments from cysteine 911 through isoleucine 929 and from isoleucine 946 through cysteine 964 in the alpha subunit of native ovine Na+/K(+)-transporting ATPase, the disposition of lysine 943 with...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00029a027
更新日期：1995-07-25 00:00:00
abstract：:Phosphorylated proteins may play an important role in regulating the metabolism or function of rod photoreceptors. In mammalian retinas, a photoreceptor protein of 33 000 (33K) molecular weight is phosphorylated in a cyclic nucleotide dependent manner in vitro. Since light initiates the activation of a photoreceptor-s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00304a014
更新日期：1984-04-24 00:00:00
abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00529
更新日期：2016-09-20 00:00:00
abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960790i
更新日期：1996-10-01 00:00:00
abstract：:Frequency-domain fluorescence resonance energy transfer and anisotropy measurements were performed to characterize conformational dynamics of an analog of the RNase S-peptide (residues 1-20). Trp was used as a donor by replacing Phe 8, and a dansyl acceptor group was introduced at position 1 or 18. The distance-distri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00097a009
更新日期：1993-11-23 00:00:00
abstract：:The binding of UDP-N-acetylglucosamine (UDPNAG) to the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) was studied in the absence and presence of the antibiotic fosfomycin by isothermal titration calorimetry. Fosfomycin binds covalently to MurA in the presence of UDPNAG and also in its absence as demonst...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0107041
更新日期：2001-08-21 00:00:00
abstract：:A specific, high-affinity binding site for ammodytoxin C in synaptic membranes from bovine cerebral cortex was detected and partially characterized. Equilibrium binding analysis revealed a single population of [125I]ammodytoxin C acceptors with the following binding parameters: Kd = 6.0 nM and Bmax = 5.7 pmol/mg membr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00250a049
更新日期：1994-11-22 00:00:00
abstract：:Crystal structures of complexes of a D30N mutant of feline immunodeficiency virus protease (FIV PR) complexed with a statine-based inhibitor (LP-149), as well as with a substrate based on a modification of this inhibitor (LP-149S), have been solved and refined at resolutions of 2.0 and 1.85 A, respectively. Both the i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9707436
更新日期：1997-09-02 00:00:00
abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963133z
更新日期：1997-05-06 00:00:00
abstract：:Malic enzyme from pigeon liver is alkylated by the substrate analogue bromopyruvate, resulting in the concomitant loss of its oxidative decarboxylase and oxalacetate decarboxylase activities, but not its ability to reduce alpha-keto acids. The inactivation of oxidative decarboxylase activity follows saturation kinetic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00621a024
更新日期：1977-01-25 00:00:00
abstract：:Information on the secondary structure and interactions of RNA is important to understand the biological function of RNA as well as in applying RNA as a tool for therapeutic purposes. Recently, the isoenergetic microarray mapping method was developed to improve the prediction of RNA secondary structure. Herein, for th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200463p
更新日期：2011-09-06 00:00:00
abstract：:Large conductance Ca(2+)-activated K(+) channels (BK(Ca)) contain an intracellular binding site for bovine pancreatic trypsin inhibitor (BPTI), a well-known inhibitor of various serine proteinase (SerP) enzymes. To investigate the structural basis of this interaction, we examined the activity of 11 BPTI mutants using ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992140v
更新日期：2000-02-29 00:00:00
abstract：:Rabbit lung flavin-containing monooxygenase (FMO, EC 1.14.13.8) was denatured, reduced, carboxymethylated, digested with endoproteinase Glu-C or trypsin, and subjected to mass spectrometric analysis. The amino acid sequences of selected peptides were determined by tandem mass spectrometry. Over 90% of rabbit lung FMO ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00105a012
更新日期：1991-10-15 00:00:00
abstract：:P700, the primary electron donor of photosystem I is an asymmetric dimer made of one molecule of chlorophyll a' (P(A)) and one of chlorophyll a (P(B)). While the carbonyl groups of P(A) are involved in hydrogen-bonding interactions with several surrounding amino acid side chains and a water molecule, P(B) does not eng...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049515j
更新日期：2004-07-06 00:00:00
abstract：:Intrastrand cross-links represent the majority of modifications in DNA resulting from interaction with the cancer chemotherapeutic drug cis-diamminedichloroplatinum(II) (cis-DDP). These adducts were recently characterized although several discrepancies remained to be resolved. In these studies, [3H]-cis-dichloro(ethyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00361a026
更新日期：1986-07-01 00:00:00
abstract：:Hexose-1-phosphate uridylyltransferase catalyzes the interconversion of UDP-galactose and glucose-1-P with UDP-glucose and galactose-1-P by a double-displacement mechanism through a covalent intermediate (E-UMP), in which UMP is bonded to one of two histidine residues at the active site, H164 or H166. To identify whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00499a003
更新日期：1990-11-27 00:00:00
abstract：:Sumoylation, the process by which the ubiquitin-related SUMO protein is covalently attached to lysine side chains in other proteins, is involved in numerous processes in the eukaryotic cell, including transcriptional repression. In this study, we identify Gcn5, the histone-modifying subunit of the transcriptional regu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051624q
更新日期：2006-01-24 00:00:00
abstract：:Peptide mapping, chemical sequencing, microbore HPLC/electrospray ionization mass spectrometry (LC/ESI/MS), and matrix-assisted laser desorption mass spectrometry (MALDI/MS) were used to identify the sites of intra- and intermolecular disulfide linkages in bovine dopamine beta-hydroxylase. The enzyme contains 14 cyste...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00204a019
更新日期：1994-09-27 00:00:00
abstract：:35S-labeled derivatives of the insecticides nodulisporic acid and ivermectin were synthesized and demonstrated to bind with high affinity to a population of receptors in Drosophila head membranes that were previously shown to be associated with a glutamate-gated chloride channel. Nodulisporic acid binding was modeled ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015920o
更新日期：2002-05-21 00:00:00
abstract：:At pH 8.9 and 37 degrees C the half-times for tritium exchange with the C-2 protons of the histidines of trypsin are 73 days for His-57, and greater than 1000 days for His-40 and His-91. These half-times are much longer than the half-life of exchange for the C-2 proton of free histidine (2.8 days at pD 8.2), and longe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00661a010
更新日期：1976-08-10 00:00:00
abstract：:The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9728699
更新日期：1998-04-28 00:00:00
abstract：:Protocatechuate 3,4-dioxygenase (3,4-PCD) utilizes a ferric ion to catalyze the aromatic ring cleavage of 3,4-dihydroxybenzoate (PCA) by incorporation of both atoms of dioxygen to yield beta-carboxy-cis, cis-muconate. The crystal structures of the anaerobic 3,4-PCD.PCA complex, aerobic complexes with two heterocyclic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970469f
更新日期：1997-08-19 00:00:00
abstract：:Chromaffin granules isolated from bovine adrenal medulla were labeled with small unilamellar vesicles (SUV) made from N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine and/or N-(lissamine rhodamine B sulfonyl)phosphatidylethanolamine as donor and acceptor fluorophores. Labeling of granules could be followed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00315a020
更新日期：1984-09-25 00:00:00
abstract：:The steady-state and pre-steady-state kinetic parameters for the interaction of E with the fluorogenic substrate 2-aminobenzoyl-Thr-Ile-Nle-Phe(p-NO(2))-Gln-Arg-NH(2) were determined in 1.25 M NaCl, 0.1 M MES-TRIS at pH 6.0 at 25 degrees C. At low concentrations of enzyme, the values of the K(m) and k(cat) calculated ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0116543
更新日期：2002-01-29 00:00:00
abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035319l
更新日期：2004-01-13 00:00:00
abstract：:The heme and cysteine microenvironments of bluefin tuna apomyoglobin have been investigated by examining the fluorescence properties of two extrinsic chromophores, i.e., ANS and 1,5-AEDANS. 1,5-AEDANS was covalently bound to the single cysteine residue found in the primary structure of tuna apomyoglobin. Recombination...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a002
更新日期：1982-01-19 00:00:00
abstract：:Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00495
更新日期：2020-09-29 00:00:00
abstract：:The fungal metabolite gliotoxin has a redox-active disulfide bridge spanning carbons 3 and 6 of a diketopiperazine (DKP) scaffold. The proposed DKP synthetase, GliP, from Aspergillus fumigatus Af293, is a three module (A1-T1-C1-A2-T2-C2-T3) 236 kDa protein that can be overproduced in soluble form in Escherichia coli. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061845b
更新日期：2006-12-19 00:00:00
abstract：:The beta subunits of the 143-kDa alpha2beta2 tetrameric enzyme tryptophan synthase have been labeled by L-[ring-4-19F]phenylalanine and L-[phenol-4-13C]tyrosine in an effort to monitor the positions of these residues on ligand binding. Of the 13 phenylalanine and 11 tyrosine residues in the beta subunit, only three pa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9518297
更新日期：1996-03-12 00:00:00
abstract：:A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00626a042
更新日期：1977-04-05 00:00:00
