Abstract：
:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced by parallel pathways of a nonsequential unfolding mechanism rather than being consecutive steps in a sequential process. One intermediate can be detected in unfolding experiments because it is kinetically trapped in that conformation, but it is not observed in refolding experiments. It has spectroscopic and hydrodynamic properties very similar to those of the native protein, but it is inactive. The other intermediate could not be characterized because it either aggregates or unfolds under our experimental conditions and could not be isolated chromatographically.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sánchez del Pino MM,Fersht ARdoi
10.1021/bi963133zsubject
Has Abstractpub_date
1997-05-06 00:00:00pages
5560-5issue
18eissn
0006-2960issn
1520-4995pii
bi963133zjournal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00187a001
更新日期：1994-05-31 00:00:00
abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980042o
更新日期：1998-07-14 00:00:00
abstract：:We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972197m
更新日期：1998-01-20 00:00:00
abstract：:Unoccupied estrogen receptor (ER) can be extracted from tissues by homogenization with a hypotonic buffer, whereas hormone-occupied ER becomes tightly bound to the nuclear pellet and must be extracted with high-salt-containing buffers. The molecular basis for estrogen-induced tight nuclear binding of ER remains an imp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982190k
更新日期：1999-06-01 00:00:00
abstract：:Only beta-beta cross-links form when the alpha(3)(betaE(395)C)(3)gammaK(36)C (MF(1) residue numbers) double mutant subcomplex of TF(1), the F(1)-ATPase from the thermophilic Bacillus PS3, is slowly inactivated with CuCl(2) in the presence or absence of MgATP. The same slow rate of inactivation and extent of beta-beta ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036058i
更新日期：2004-03-09 00:00:00
abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026191v
更新日期：2002-10-15 00:00:00
abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00656a008
更新日期：1976-06-01 00:00:00
abstract：:The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301483z
更新日期：2013-01-08 00:00:00
abstract：:Stopped-flow measurements of protein fluorescence quenching when methotrexate (MTX) binds to dihydrofolate reductase (isoenzyme II) of Streptococcus faecium (SFDHFR II) analyze as the sum of two differentials: a rapid binding phase and a second phase for which the observed rate constant is independent of methotrexate ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00339a010
更新日期：1985-08-27 00:00:00
abstract：:Under conditions where muscle actin only partially polymerizes, or where it does not polymerize at all, a significant enhancement of polymerization was observed if equimolar phalloidin was also present. The increased extent of polymerization in the the presence of phalloidin can be explained by the reduced critical ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00507a006
更新日期：1981-02-17 00:00:00
abstract：:A positional-scanning combinatorial protocol was employed to optimize the deoxyribose-based cleavage of B-form DNA by Ni(II) x Xaa-Xaa-His metallopeptides. This procedure employed 18 naturally occurring amino acids (excluding Cys and Trp) to generate two libraries in which the first and second positions of the peptide...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982587o
更新日期：1999-02-16 00:00:00
abstract：:We previously showed that beta-amyloid precursor protein (APP) is cleaved not only in the middle of the membrane (gamma-cleavage) but also at novel cleavage sites close to the membrane/cytoplasmic boundary (epsilon-cleavage), releasing APP intracellular domains (AICDs) 49-99 and 50-99. To learn more about the relation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049399k
更新日期：2004-10-26 00:00:00
abstract：:5,6-Dihydrothymidine 5'-triphosphate (DHdTTP) was synthesized by catalytic hydrogenation of thymidine 5'-triphosphate (dTTP). Thymidine glycol 5'-triphosphate (dTTP-GLY) was prepared by bromination of dTTP followed by treatment with Ag2O. The modified nucleotides were extensively purified by anion-exchange high-perfor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00377a042
更新日期：1987-02-10 00:00:00
abstract：:The doublet at 850 and 830 cm-1 in the Raman spectra of proteins containing tyrosyl residues has been examined as to its origin and the relation of its components to the environment of the phenyl ring, the state of the phenolic hydroxyl group, and the conformation of the amino acid backbone. Raman spectral studies on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00693a014
更新日期：1975-11-04 00:00:00
abstract：:Mammalian type B (mitochondrial) b(5) cytochromes exhibit greater amino acid sequence diversity than their type A (microsomal) counterparts, as exemplified by the type B proteins from human (hCYB5B) and rat (rCYB5B). The comparison of X-ray crystal structures of hCYB5B and rCYB5B reported herein reveals a striking dif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2004729
更新日期：2011-06-21 00:00:00
abstract：:We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00170a011
更新日期：1994-02-01 00:00:00
abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a005
更新日期：1988-07-12 00:00:00
abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960971k
更新日期：1996-12-03 00:00:00
abstract：:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00350
更新日期：2017-07-25 00:00:00
abstract：:Achieving a satisfactory biochemical explanation for the opportunistic underwater adhesion of marine invertebrates such as mussels and barnacles requires a detailed characterization of proteins extracted from holdfast structures produced by these organisms. Mefp-5 is an adhesive protein derived from the foot of the co...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002718x
更新日期：2001-03-06 00:00:00
abstract：:The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00083a038
更新日期：1993-08-17 00:00:00
abstract：:The role of the tryptophan residues in the substrate-binding and catalytic mechanism of an enzymatically active C-terminal fragment of Pseudomonas aeruginosa exotoxin A was studied by individually or jointly replacing these residues with phenylalanine. Substitution of W-466 decreased the ADP-ribosyltransferase and NAD...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961985t
更新日期：1996-12-03 00:00:00
abstract：:Regulation of adenylate cyclase by alpha 2 adrenergic receptors requires the inhibitory guanine nucleotide binding protein Ni. A role for this protein has also been suggested in the high-affinity binding of agonists to the alpha 2 receptor. We recently reported that alkaline treatment can selectively inactivate alpha ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a061
更新日期：1987-06-16 00:00:00
abstract：:Kinetic parameters for triosephosphate isomerase catalysis of the elimination reaction of an equilibrium mixture of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (DGAP) to form methylglyoxal and phosphate ion are reported for the enzyme from rabbit muscle. Pseudo-first-order rate constants for the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00232a031
更新日期：1991-05-07 00:00:00
abstract：:The calcium-dependent interaction of several annexins with membranes was studied. A novel technique was developed that allowed estimation of calcium binding to aggregating systems. This consisted of immobilized phospholipids on phenyl-Sepharose. Proteins associated with this affinity gel in the presence of radioactive...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00249a009
更新日期：1994-11-15 00:00:00
abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00098a007
更新日期：1991-08-27 00:00:00
abstract：:While the urea-mediated unfolding pathway of the Escherichia coli aspartate aminotransferase (eAATase) homodimer proceeds through a reversible three-state process with a partially folded dimeric intermediate, D D* 2U (E. Deu and J. F. Kirsch, accompanying paper), that of a cofactor-stabilized form differs. Pyridoxal p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602632d
更新日期：2007-05-15 00:00:00
abstract：:Two nucleoside diphosphate analogs, 3'-C-methyl-ADP and 3'-C-methyl-UDP, have been tested as substrate and/or allosteric effectors using the adenosylcobalamin-dependent ribonucleoside diphosphate reductase of Corynebacterium nephridii. Neither analog was a substrate for the reductase. However, they did function as all...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00160a035
更新日期：1992-11-17 00:00:00
abstract：:A specific requirement for lipids, particularly cardiolipin (CL), in cytochrome c oxidase (CcO) has been reported in many previous studies using mainly in vitro lipid removal approaches in mammalian systems. Our accompanying paper shows that CcO produced in markedly CL-depleted Rhodobacter sphaeroides displays wild-ty...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1017039
更新日期：2011-05-17 00:00:00
abstract：:Six rhodopsin mutants containing disulfide cross-links between different cytoplasmic regions were prepared: disulfide bond 1, between Cys65 (interhelical loop I-II) and Cys316 (end of helix VII); disulfide bond 2, between Cys246 (end of helix VI) and Cys312 (end of helix VII); disulfide bond 3, between Cys139 (end of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9912443
更新日期：1999-09-28 00:00:00
