Abstract:
:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal pentapeptide by reversed-phase high-performance liquid chromatography. EGF-T competes for binding to EGF receptors with the same efficiency as EGF. The EGF-T2 derivative had no detectable receptor binding activity even at 100 nM. The in vitro mitogenic potencies of EGF and EGF-T for Balb/c 3T3 cells were indistinguishable. Treatment of EGF-T with carboxypeptidase Y yielded two derivatives, EGF-T-(des-Arg48) and EGF-T-des(Leu47-Arg48). There was only a 3-7-fold diminution in the binding efficiency and mitogenic potency for EGF-T-(des-Arg48). However, there was more than a 100-fold decrease in the binding efficiency and mitogenic activity of EGF-T-des (Leu47-Arg48). These results indicated that Leu47 is intimately involved in the formation of the ligand-receptor complex. Studies with a number of proteases indicated that the C-terminus of EGF was susceptible to enzymic digestion; however, the N-terminus appears to be folded into a conformation which prevents access to proteolytic digestion. Consequently, the N-terminus was modified by preparing an analogue with recombinant DNA technology. Oligonucleotides corresponding to EGF(3-48). Met3 Lys21 residues were ligated in frame to a beta-galactosidase expression vector. The beta-Gal-EGF fusion protein was cleaved with cyanogen bromide and EGF(4-48).Lys21 purified.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Burgess AW,Lloyd CJ,Smith S,Stanley E,Walker F,Fabri L,Simpson RJ,Nice ECdoi
10.1021/bi00414a005subject
Has Abstractpub_date
1988-07-12 00:00:00pages
4977-85issue
14eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::To describe the hydrophobic adsorption of charged molecules to bilayer membranes, one must recognize that the adsorption produces a change in the electrostatic potential at the surface of the membrane. The surface potential produced by the adsorption of the charged molecules can be described most simply by the Gouy eq...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00654a023
更新日期:1976-05-04 00:00:00
abstract::Cibacron Blue F3GA dye has been used to probe subtle conformational changes in protein structure associated with the conversion of Escherichia coli glutamine synthetase (GS) between relaxed, taut, oxidized, and dissociated forms. Binding of the dye to each form of the enzyme elicits a different spectral perturbation o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00324a017
更新日期:1985-01-29 00:00:00
abstract::Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0155201
更新日期:2001-07-24 00:00:00
abstract::The kcat and Km values for the bovine heart low molecular weight phosphotyrosyl protein phosphatase catalyzed hydrolysis of 16 aryl phosphate monoesters and of five alkyl phosphate monoesters having the structure Ar(CH2)nOPO3H2 (n = 1-5) were measured at pH 5.0 and 37 degrees C. With the exception of alpha-naphthyl ph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a006
更新日期:1991-09-17 00:00:00
abstract::The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00557a023
更新日期:1980-08-05 00:00:00
abstract::Human granulocyte chemotactic protein 2 (GCP-2) has originally been isolated from cytokine-stimulated osteosarcoma cells as a chemokine coproduced in minute amounts together with interleukin 8. Human GCP-2 (75 residues) was synthesized on a 0.25-mmol scale using Fmoc chemistry. After disulfide bridge formation and pur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961999z
更新日期:1997-03-04 00:00:00
abstract::The covalent affinity probe maltosyl isothiocyanate (MITC) has been used previously to identify the glucose transporter of human erythrocytes as a component of band 3. By use of limited proteolysis, the site on the Mr 100 000 protein to which MITC attaches has been localized to a 17 000-dalton region near the center o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00331a001
更新日期:1985-05-07 00:00:00
abstract::The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701645g
更新日期:2007-12-11 00:00:00
abstract::Fatty acid-induced upregulation of death receptor 5 (DR5) and its cognate ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), promotes hepatocyte lipoapoptosis, which is a key mechanism in the progression of fatty liver disease. Accordingly, inhibition of DR5 signaling represents an attractive str...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00529
更新日期:2020-10-13 00:00:00
abstract::Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801047q
更新日期:2008-09-16 00:00:00
abstract::The solution structures of isolated monomeric actins in their Mg(2+)-ATP and Ca(2+)-ATP bound forms and in complexes with gelsolin segment-1 have been probed using hydroxyl radicals (*OH) generated by synchrotron X-ray radiolysis. Proteolysis and mass spectrometry analysis of 28 peptides containing 58 distinct reactiv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034914k
更新日期:2003-10-21 00:00:00
abstract::A complex of photoreceptor phoborhodopsin (ppR; also called sensory rhodopsin II) and its cognate halobacterial transducer II (pHtrII) existing in the plasma membrane mediates the light signal to the cytoplasm in the earliest step of negative phototaxis in Natronomonas pharaonis. We have investigated the dynamics of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602482s
更新日期:2007-05-08 00:00:00
abstract::The activity of the major isoform of porcine pancreatic phospholipase A2 (PLA2), designated B-PLA2, against micellar substrates is inhibited by heparin. Inhibition is a consequence of binding of the enzyme to heparin, documented by a heparin-induced alteration in the intrinsic fluorescence of B-PLA2 and in the 8-anili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a026
更新日期:1991-09-17 00:00:00
abstract::Human heart short chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD) catalyzes the oxidation of the hydroxyl group of L-3-hydroxyacyl-CoA to a keto group, concomitant with the reduction of NAD+ to NADH, as part of the beta-oxidation pathway. The homodimeric enzyme has been overexpressed in Escherichia coli, purified to h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9829027
更新日期:1999-05-04 00:00:00
abstract::The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00385a045
更新日期:1987-06-02 00:00:00
abstract::Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0505215
更新日期:2005-08-09 00:00:00
abstract::(E)-4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH or LytB) catalyzes the terminal step of the MEP/DOXP pathway where it converts (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into the two products, isopentenyl diphosphate and dimethylallyl diphosphate. The reaction involves the reductive elimination ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3001215
更新日期:2012-06-19 00:00:00
abstract::The cytokinin-active isoprenoid nucleosides of Agrobacterium tumefaciens transfer ribonucleic acid were identified by high-pressure liquid chromatography, permethylation, and mass spectroscopy. Besides the expected 6-[(3-methylbut-2-enyl)amino]-9-(beta-D-ribofuranosyl)purine (i6A) and its 2-methylthio derivative (ms2i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00524a014
更新日期:1981-10-13 00:00:00
abstract::Imidazolonepropionase (HutI) (imidazolone-5-propanote hydrolase, EC 3.5.2.7) is a member of the amidohydrolase superfamily and catalyzes the conversion of imidazolone-5-propanoate to N-formimino-L-glutamate in the histidine degradation pathway. We have determined the three-dimensional crystal structures of HutI from A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800180g
更新日期:2008-05-20 00:00:00
abstract::When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and fol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00254a021
更新日期:1994-12-20 00:00:00
abstract::The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801651w
更新日期:2009-01-13 00:00:00
abstract::We report differences in the interaction of two structurally similar phenanthroline intercalators, ethidium and propidium, with poly(dA).poly(dT) and poly[d(A-T)] as a function of ionic strength based on titration microcalorimetry, fluorescence titration, and hydrostatic pressure measurements. Both ethidium and propid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00472a009
更新日期:1990-05-22 00:00:00
abstract::A grand canonical formalism is developed to combine discrete simulations for chemically distinct species in equilibrium. Each simulation is based on a perturbed funneled landscape. The formalism is illustrated using the alkaline-induced transitions of cytochrome c as observed by FTIR spectroscopy and with various othe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802293m
更新日期:2009-03-24 00:00:00
abstract::DREAM (also known as K(+) channel interacting protein 3 and calsenilin) is a calcium binding protein and an active modulator of KV4 channels in neuronal cells as well as a novel Ca(2+)-regulated transcriptional modulator. DREAM has also been associated with the regulation of Alzheimer's disease through the prevention ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00067
更新日期:2016-03-29 00:00:00
abstract::A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034958t
更新日期:2003-09-16 00:00:00
abstract::Histone H3 arginine 2 (H3R2) is post-translationally modified in three different states by "writers" of the protein arginine methyltransferase (PRMT) family. H3R2 methylarginine isoforms include PRMT5-catalyzed monomethylation (me1) and symmetric dimethylation (me2s) and PRMT6-catalyzed me1 and asymmetric dimethylatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00035
更新日期:2020-10-06 00:00:00
abstract::Sequence-specific DNA binding proteins generally consist of more than two DNA-contacting regions to ensure the selectivity of recognition. The multiple DNA binding modules are connected either through the covalent linker or through the noncovalent dimerization domain. We have compared the DNA binding of peptide dimers...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981743o
更新日期:1999-02-02 00:00:00
abstract::We propose a rapid chemical strategy for identifying base pairs in structured nucleic acid systems. The approach goes beyond traditional chemical mapping approaches by monitoring perturbations of each residue's chemical accessibility in response to systematic mutagenesis of residues that are distant in sequence but ne...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101123g
更新日期:2010-09-07 00:00:00
abstract::A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a043
更新日期:1989-08-22 00:00:00
abstract::O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960971k
更新日期:1996-12-03 00:00:00