当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Structural reorganization of proteins revealed by radiolysis and mass spectrometry: G-actin solution structure is divalent cation dependent.

Structural reorganization of proteins revealed by radiolysis and mass spectrometry: G-actin solution structure is divalent cation dependent.

Abstract：

:The solution structures of isolated monomeric actins in their Mg(2+)-ATP and Ca(2+)-ATP bound forms and in complexes with gelsolin segment-1 have been probed using hydroxyl radicals (*OH) generated by synchrotron X-ray radiolysis. Proteolysis and mass spectrometry analysis of 28 peptides containing 58 distinct reactive probe sites within actin were used to monitor conformational variations linked to divalent cation and gelsolin segment-1 binding. The solvent accessibilities of the probe sites, as measured by footprinting in solution for the Ca(2+)-G-actin and Mg(2+)-G-actin complexes with gelsolin segment-1, were consistent with available crystallographic data. This included a specific protection at the contact interface between the partners, as revealed by reduced reactivity of peptide 337-359 in the complex. Aside from the specific protection indicated previously, the oxidation rates for the reactive residues of the isolated Ca(2+)-G-actin were similar to those of the actin gelsolin segment-1 complexes; however, the reactivity of numerous residues in the isolated Mg(2+)-G-actin form was significantly reduced. Specifically, Mg(2+)-G-actin has a set of protected sites relative to Ca(2+)-G-actin that suggest a structural reorganization in subdomains 4 and 2 and a C-terminus more closely packed onto subdomain 1. These conformational variations for isolated Mg(2+)-G-actin provide a structural basis for its greater tendency to polymerize into filaments as compared to Ca(2+)-G-actin.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Guan JQ,Almo SC,Reisler E,Chance MR

doi

10.1021/bi034914k

subject

Has Abstract

pub_date

2003-10-21 00:00:00

pages

11992-2000

issue

41

eissn

0006-2960

issn

1520-4995

journal_volume

42

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Peptoid-Substituted Hybrid Antimicrobial Peptide Derived from Papiliocin and Magainin 2 with Enhanced Bacterial Selectivity and Anti-inflammatory Activity.

    abstract：:Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly Papilio xuthus. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog Xenopus laevis. We designed an 18-residue hybrid pep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00392

    authors： Shin A,Lee E,Jeon D,Park YG,Bang JK,Park YS,Shin SY,Kim Y

    更新日期：2015-06-30 00:00:00

  • Reactivity of fibrinogen and fibrinopeptide A containing fibrinogen fragments with antisera to fibrinopeptide A.

    abstract：:Two antisera used in the radioimmunoassay for human fibrinopeptide A (FPA) which appear to have different immunochemical specificities have been tested for cross-reactivity with fibrinogen and with three fragments of fibrinogen which contain the FPA sequence. These fragments were the three-chain, NH2-terminal disulfid...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00651a004

    authors： Canfield RE,Dean J,Nossel HL,Butler VP Jr,Wilner GD

    更新日期：1976-03-23 00:00:00

  • Digestion of RNA of chromatin and nuclear ribonucleoprotein by staphylococcal nuclease.

    abstract：:Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00662a026

    authors： Augenlicht LH,McCormick M,Lipkin M

    更新日期：1976-08-24 00:00:00

  • Catalytically active cross-species heterodimers of thymidylate synthase.

    abstract：:Thymidylate synthase (TS) is a highly conserved homodimeric enzyme with two active sites, each of which contains amino acid residues from both subunits. We show that the conservation at the subunit interface between Escherichia coli TS and Lactobacillus casei TS is sufficient to permit the formation of a cross-species...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00090a002

    authors： Greene PJ,Maley F,Pedersen-Lane J,Santi DV

    更新日期：1993-10-05 00:00:00

  • Cadmium-113 NMR studies of the DNA binding domain of the mammalian glucocorticoid receptor.

    abstract：:The DNA binding domain of the mammalian glucocorticoid hormone receptor (GR) contains nine highly conserved cysteine residues, a conservation shared by the superfamily of steroid and thyroid hormone receptors. A fragment [150 amino acids (AA) in length] consisting of GR residues 407-556, containing within it the entir...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00491a016

    authors： Pan T,Freedman LP,Coleman JE

    更新日期：1990-10-02 00:00:00

  • Divergence in nonspecific hydrophobic packing interactions in the apo state, and its possible role in functional specialization of mitochondrial and microsomal cytochrome b5.

    abstract：:The outer mitochondrial membrane isoform of mammalian cytochrome b(5) (OM b(5)) is distinguished from the microsomal isoform (Mc b(5)) by its considerably greater stability. In contrast, OM and Mc apocytochrome b(5) (apo-b(5)) exhibit similar thermodynamic stability. Contributing substantially to the greater stability...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051337m

    authors： Cowley AB,Sun N,Rivera M,Benson DR

    更新日期：2005-11-08 00:00:00

  • Substrate specificity of the heparan sulfate hexuronic acid 2-O-sulfotransferase.

    abstract：:The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002926p

    authors： Rong J,Habuchi H,Kimata K,Lindahl U,Kusche-Gullberg M

    更新日期：2001-05-08 00:00:00

  • Direct determination of the association constant between elongation factor Tu X GTP and aminoacyl-tRNA using fluorescence.

    abstract：:We have investigated the formation of the aa-tRNA X EF-Tu X GTP ternary complex spectroscopically by monitoring a fluorescence change that accompanies the association of EF-Tu X GTP with Phe-tRNAPhe-F8, a functionally active analogue of Phe-tRNAPhe with a fluorescein moiety covalently attached to the s4U-8 base. With ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00324a023

    authors： Abrahamson JK,Laue TM,Miller DL,Johnson AE

    更新日期：1985-01-29 00:00:00

  • Phosphorylation of the gamma subunit of the retinal photoreceptor cGMP phosphodiesterase by the cAMP-dependent protein kinase and its effect on the gamma subunit interaction with other proteins.

    abstract：:Cyclic GMP phosphodiesterase, a key enzyme in phototransduction, is composed of P alpha beta and two P gamma subunits. Interaction of P gamma with P alpha beta or with the alpha subunit (T alpha) of transducin is crucial for the regulation of cGMP phosphodiesterase in retinal photoreceptors. Here we have investigated ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973087i

    authors： Xu LX,Tanaka Y,Bonderenko VA,Matsuura I,Matsumoto H,Yamazaki A,Hayashi F

    更新日期：1998-04-28 00:00:00

  • A mechanism for hydroxylation by tyrosine hydroxylase based on partitioning of substituted phenylalanines.

    abstract：:The iron-containing enzyme tyrosine hydroxylase catalyzes the hydroxylation of tyrosine to dihydroxyphenylalanine. A series of 4-X-substituted (X = H, F, Br, Cl, CH3, or CH3O) phenylalanines have been characterized as substrates to gain insight into the mechanism of hydroxylation. Multiple hydroxylated products were f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9606861

    authors： Hillas PJ,Fitzpatrick PF

    更新日期：1996-06-04 00:00:00

  • Long-range structural and dynamical changes induced by cofactor binding in DNA methyltransferase M.HhaI.

    abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602662e

    authors： Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

    更新日期：2007-06-19 00:00:00

  • Effect of lipid composition on the calcium/adenosine 5'-triphosphate coupling ratio of the Ca2+-ATPase of sarcoplasmic reticulum.

    abstract：:The Ca2+-ATPase of sarcoplasmic reticulum was purified and depleted of proteolipids by solubilization in Triton X-100 and by fractionation on a DE-52 column. The protein reconstituted by deoxycholate-cholate dialysis at low lipid to protein ratios (2-5 mg of lipid/mg of protein), with either dioleoylphosphatidylethano...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00296a021

    authors： Navarro J,Toivio-Kinnucan M,Racker E

    更新日期：1984-01-03 00:00:00

  • Thiol regulation of the thylakoid electron transport chain--a missing link in the regulation of photosynthesis?

    abstract：:Avoidance of over-reduction of the chloroplast ferredoxin pool is of paramount importance for plants in avoiding oxidative stress. The redox state of this pool can be controlled through regulation of the thylakoid electron transport chain. A model is presented for regulation of this chain via a thiol reduction mechani...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi027011k

    authors： Johnson GN

    更新日期：2003-03-18 00:00:00

  • Transition state in DNA polymerase β catalysis: rate-limiting chemistry altered by base-pair configuration.

    abstract：:Kinetics studies of dNTP analogues having pyrophosphate-mimicking β,γ-pCXYp leaving groups with variable X and Y substitution reveal striking differences in the chemical transition-state energy for DNA polymerase β that depend on all aspects of base-pairing configurations, including whether the incoming dNTP is a puri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500101z

    authors： Oertell K,Chamberlain BT,Wu Y,Ferri E,Kashemirov BA,Beard WA,Wilson SH,McKenna CE,Goodman MF

    更新日期：2014-03-25 00:00:00

  • Methyl-accepting chemotaxis proteins of Escherichia coli: methylated at three sites in a single tryptic fragment.

    abstract：:The location of the sites of methylation of the methyl-accepting chemotaxis proteins (MCP) of Escherichia coli has been investigated by trypsin digestion and mapping of the peptides. The two principal MCPs, MCP I and MCP II, were found to have very similar methyl-labeled peptide patterns. Each produced three major spe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00507a051

    authors： Chelsky D,Dahlquist FW

    更新日期：1981-02-17 00:00:00

  • The crystal structure of eosinophil cationic protein in complex with 2',5'-ADP at 2.0 A resolution reveals the details of the ribonucleolytic active site.

    abstract：:Eosinophil cationic protein (ECP) is a component of the eosinophil granule matrix. It shows marked toxicity against helminth parasites, bacteria single-stranded RNA viruses, and host epithelial cells. Secretion of human ECP is related to eosinophil-associated allergic, asthmatic, and inflammatory diseases. ECP belongs...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0264521

    authors： Mohan CG,Boix E,Evans HR,Nikolovski Z,Nogués MV,Cuchillo CM,Acharya KR

    更新日期：2002-10-08 00:00:00

  • Thermodynamics of ligand binding to trp repressor.

    abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00079a029

    authors： Jin L,Yang J,Carey J

    更新日期：1993-07-20 00:00:00

  • Higher-order association states of cellular ERBB3 probed with photo-cross-linkable aptamers.

    abstract：:Nucleic acid aptamers are rapidly gaining prominence as diagnostic tools, targeting reagents, and potential therapeutics. To extend the use of aptamers into the biochemical analysis of protein interactions on the surface of live cells, we converted an enzymatically generated RNA aptamer into a photo-cross-linkable aff...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8004208

    authors： Park E,Baron R,Landgraf R

    更新日期：2008-11-18 00:00:00

  • Hydrophobic core of the steroidogenic acute regulatory protein for cholesterol transport.

    abstract：:The steroidogenic acute regulatory protein (StAR), the first family member of START (StAR-related lipid transport) proteins, plays an essential role by facilitating the movement of cholesterol from the outer to inner mitochondrial membrane. Wild-type and mutant StAR binds cholesterol with similar intensity, but only w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801514e

    authors： Bose HS,Whittal RM,Bose M,Debnath D

    更新日期：2009-02-17 00:00:00

  • Human transforming growth factor type beta 2: production by a prostatic adenocarcinoma cell line, purification, and initial characterization.

    abstract：:Human type beta 2 transforming growth factor (hTGF-beta 2) was purified from tamoxifen-supplemented, serum-free medium conditioned by the human prostatic adenocarcinoma cell line PC-3. The purification of hTGF-beta 2 was monitored in a growth inhibition assay and was achieved by batch purification on methylsilyl-contr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00383a002

    authors： Ikeda T,Lioubin MN,Marquardt H

    更新日期：1987-05-05 00:00:00

  • Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms.

    abstract：:Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprote...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902130j

    authors： Zhi L,Mans J,Paskow MJ,Brown PH,Schuck P,Jonjić S,Natarajan K,Margulies DH

    更新日期：2010-03-23 00:00:00

  • Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer.

    abstract：:The velocity of the oxidative renaturation of reduced ribonuclease A catalyzed by protein disulfide isomerase (PDI) is strongly dependent on the composition of a glutathione/glutathione disulfide redox buffer. As with the uncatalyzed, glutathione-mediated oxidative folding of ribonuclease, the steady-state velocity of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00217a004

    authors： Lyles MM,Gilbert HF

    更新日期：1991-01-22 00:00:00

  • Modification of Escherichia coli ribosomes with the fluorescent reagent N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid. Identification of derivatized L31' and studies on its intraribosomal properties.

    abstract：:N-[[(Iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid (IAEDANS) is a fluorescent reagent which reacts covalently with the free thiol groups of proteins. When the reagent is reacted with the Escherichia coli ribosome under mild conditions, gel electrophoresis shows modification of predominantly two proteins, S18...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00346a042

    authors： Hanas JS,Simpson MV

    更新日期：1985-12-03 00:00:00

  • Mammalian glycinamide ribonucleotide transformylase: purification and some properties.

    abstract：:Glycinamide ribonucleotide transformylase, the first of the two formyl group transferases of de novo purine biosynthesis requiring 10-formyltetrahydrofolate, has been purified 1500-fold, nearly to homogeneity, from the murine lymphoma cell line L5178Y. Purification of the enzyme was facilitated by the use of a gelatin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00327a008

    authors： Caperelli CA

    更新日期：1985-03-12 00:00:00

  • 6-(Methoxymethylene)penicillanic acid: inactivator of RTEM beta-lactamase from Escherichia coli.

    abstract：:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00319a025

    authors： Brenner DG,Knowles JR

    更新日期：1984-11-20 00:00:00

  • Switching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase.

    abstract：:Many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. Recently, we showed that a single amino acid substitution in the alcohol binding pocket, L29P, in Pseudomonas fluorescens (SIK WI) aryl esterase (PFE) increased the specificity constant of P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9021268

    authors： Yin DL,Bernhardt P,Morley KL,Jiang Y,Cheeseman JD,Purpero V,Schrag JD,Kazlauskas RJ

    更新日期：2010-03-09 00:00:00

  • Redox modulation of cyclic electron flow around photosystem I in C3 plants.

    abstract：:We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061439s

    authors： Breyton C,Nandha B,Johnson GN,Joliot P,Finazzi G

    更新日期：2006-11-14 00:00:00

  • Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase.

    abstract：:Biotin synthase catalyzes the conversion of dethiobiotin (DTB) to biotin through the oxidative addition of sulfur between two saturated carbon atoms, generating a thiophane ring fused to the existing ureido ring. Biotin synthase is a member of the radical SAM superfamily, composed of enzymes that reductively cleave S-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201042r

    authors： Taylor AM,Stoll S,Britt RD,Jarrett JT

    更新日期：2011-09-20 00:00:00

  • Cross-linked electron transfer complex between cytochrome c2 and the photosynthetic reaction center of Rhodobacter sphaeroides.

    abstract：:Electron donation from the soluble cytochrome (cyt) c2 to the photooxidized primary donor, P+, of reaction centers isolated from Rhodobacter sphaeroides was studied by using chemical zero-length cross-linking. This cross-linking stabilizes a 1:1 covalent complex between subunit M of the reaction center and cyt c2. In ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961350u

    authors： Drepper F,Dorlet P,Mathis P

    更新日期：1997-02-11 00:00:00

  • Effects of ouabain on the rotational dynamics of renal Na,K-ATPase studied by saturation-transfer EPR.

    abstract：:The interaction of a nitroxide spin-labeled derivative of ouabain with sheep kidney Na,K-ATPase and the motional behavior of the ouabain spin label-Na,K-ATPase complex have been studied by means of electron paramagnetic resonance (EPR) and saturation-transfer EPR (ST-EPR). Spin-labeled ouabain binds with high affinity...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00122a019

    authors： Mahaney JE,Grisham CM

    更新日期：1992-02-25 00:00:00