Abstract:
:We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the presence of supercomplexes related to cyclic electron flow, making previous structural explanations for the modulation of cyclic flow rather unlikely. On the other hand, we found that the fraction of photosystem I complexes engaged in cyclic flow could be modulated by changes in the redox state of the chloroplast stroma. Our findings support therefore a dynamic model for the occurrence of linear and cyclic electron flow in C3 plants, based on the competition between cytochrome b(6)f and FNR for electrons carried by ferredoxin. This would be ultimately regulated by the balance between the redox state of PSI acceptors and donors during photosynthesis, in a diffusing system.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Breyton C,Nandha B,Johnson GN,Joliot P,Finazzi Gdoi
10.1021/bi061439ssubject
Has Abstractpub_date
2006-11-14 00:00:00pages
13465-75issue
45eissn
0006-2960issn
1520-4995journal_volume
45pub_type
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