Avian synaptopodin 2 (fesselin) stabilizes myosin filaments and actomyosin in the presence of ATP.

abstract：:The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...

journal_title：Biochemistry

authors： Lin SH,Lee JC

更新日期：2002-10-01 00:00:00

abstract：:Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...

journal_title：Biochemistry

authors： Giangiacomo KM,Sugg EE,Garcia-Calvo M,Leonard RJ,McManus OB,Kaczorowski GJ,Garcia ML

更新日期：1993-03-09 00:00:00

abstract：:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...

journal_title：Biochemistry

authors： Buchbinder JL,Stephenson RC,Dresser MJ,Pitera JW,Scanlan TS,Fletterick RJ

更新日期：1998-04-14 00:00:00

abstract：:Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...

journal_title：Biochemistry

authors： Spoonamore JE,Bandarian V

更新日期：2008-02-26 00:00:00

abstract：:Surface plasmon resonance (SPR) detection technology was employed to investigate the kinetic mechanism of deoxyadenosine kinase from Mycoplasma mycoides ssp. mycoides SC. In our experimental approach, the enzyme was attached to the sensor surface, the reactants were injected in the mobile phase, and the product-enzyme...

journal_title：Biochemistry

authors： Pol E,Wang L

更新日期：2006-01-17 00:00:00

abstract：:Terminase enzymes are responsible for "packaging" of viral DNA into a preformed procapsid. Bacteriophage lambda terminase is composed of two subunits, gpA and gpNu1, in a gpA(1).gpNu1(2) holoenzyme complex. The larger gpA subunit is responsible for preparation of viral DNA for packaging, and is central to the packagin...

journal_title：Biochemistry

authors： Gaussier H,Ortega ME,Maluf NK,Catalano CE

更新日期：2005-07-19 00:00:00

abstract：:The human acid sphingomyelinase (ASM, EC 3.1.4.12), a lysosomal and secretory protein coded by the sphingomyelin phosphodiesterase 1 (SMPD-1) gene, catalyzes the degradation of sphingomyelin (SM) to ceramide and phosphorylcholine. We examined the structural-functional properties of its carboxyl-terminus (amino acids 4...

journal_title：Biochemistry

authors： Lee CY,Tamura T,Rabah N,Lee DY,Ruel I,Hafiane A,Iatan I,Nyholt D,Laporte F,Lazure C,Wada I,Krimbou L,Genest J

更新日期：2007-12-25 00:00:00

abstract：:Escherichia coli glycerol kinase (EC 2.7.1.30; ATP:glycerol 3-phosphotransferase) is a key element in a signal transduction pathway that couples expression of genes required for glycerol metabolism to the relative availability of glycerol and glucose. Its catalytic activity is inhibited by protein-protein interactions...

journal_title：Biochemistry

authors： Liu WZ,Faber R,Feese M,Remington SJ,Pettigrew DW

更新日期：1994-08-23 00:00:00

abstract：:Photosystem II catalyzes the light-driven oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. The manganese stabilizing protein (MSP) of photosystem II is an extrinsic subunit that plays an important role in catalytic activity. This subunit can be extracted and re-bound to the photosystem II ...

journal_title：Biochemistry

authors： Hutchison RS,Betts SD,Yocum CF,Barry BA

更新日期：1998-04-21 00:00:00

abstract：:1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measur...

journal_title：Biochemistry

authors： Atkins AR,Martin RC,Smith R

更新日期：1995-02-14 00:00:00

abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

journal_title：Biochemistry

authors： Dewey TG,Turner DH

更新日期：1980-04-15 00:00:00

abstract：:The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGU...

journal_title：Biochemistry

authors： Klosterman PS,Shah SA,Steitz TA

更新日期：1999-11-09 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...

journal_title：Biochemistry

authors： Rockwell NC,Martin SS,Lagarias JC

更新日期：2012-05-01 00:00:00

abstract：:The effect of ionic strength, temperature, and divalent cations on the association of myosin with actin was determined in the ultracentrifuge using scanning absorption optics. The association constant (Ka) for the binding of heavy meromyosin (HmM) to F-actin was 1 X 10(7) M-1 at 20 degrees C, in 0.10 M KCl, 0.01 M imi...

journal_title：Biochemistry

authors： Margossian SS,Lowey S

更新日期：1978-12-12 00:00:00

abstract：:Dihydrofolate reductase (DHFR) is often used as a model system to study the relation between protein dynamics and catalysis. We have studied a number of variants of the cold-adapted DHFR from Moritella profunda (MpDHFR), in which the catalytically important M20 and FG loops have been altered, and present a comparison ...

journal_title：Biochemistry

authors： Behiry EM,Evans RM,Guo J,Loveridge EJ,Allemann RK

更新日期：2014-07-29 00:00:00

abstract：:The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs. MutY also exhibits adenine glycosylase activity toward...

journal_title：Biochemistry

authors： Porello SL,Leyes AE,David SS

更新日期：1998-10-20 00:00:00

abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...

journal_title：Biochemistry

authors： Haunerland NH,Jacobson BL,Wesenberg G,Rayment I,Holden HM

更新日期：1994-10-18 00:00:00

abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

journal_title：Biochemistry

authors： Beard WA,Wilson SH

更新日期：2014-05-06 00:00:00

abstract：:2-Azidoadenosine 5'-diphosphate (2-azido-ADP) labeled with 32P in the alpha-position was prepared and used to photolabel the nucleotide binding sites of beef heart mitochondrial F1-ATPase. The native F1 prepared by the procedure of Knowles and Penefsky [Knowles, A. F., & Penefsky, H. S. (1972) J. Biol. Chem. 247, 6617...

journal_title：Biochemistry

authors： Boulay F,Dalbon P,Vignais PV

更新日期：1985-12-03 00:00:00

abstract：:The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...

journal_title：Biochemistry

authors： Dwulet FE,Bogardt RA,Jones BN,Lehman LD,Gurd FR

更新日期：1975-12-02 00:00:00

abstract：:The bovine prothrombin gene was characterized by Southern blot analysis of bovine genomic DNA using bovine prothrombin cDNA fragments as hybridization probes. These analyses suggested that the bovine genome contains a single prothrombin gene that is at least 10 kilobase pairs (kbp) in size. To characterize the gene mo...

journal_title：Biochemistry

authors： Irwin DM,Ahern KG,Pearson GD,MacGillivray RT

更新日期：1985-11-19 00:00:00

abstract：:Nuclear magnetic resonance (NMR) has been used to monitor the conformation and dynamics of the d(C1-G2-A3-T4-T5-A6-T6-A5-A4-T3-C2-G1) self-complementary dodecanucleotide duplex (henceforth called Pribnow 12-mer), which contains a TATAAT Pribnow box and a central core of eight dA X dT base pairs. The exchangeable imino...

journal_title：Biochemistry

authors： Patel DJ,Kozlowski SA,Weiss M,Bhatt R

更新日期：1985-02-12 00:00:00

abstract：:To probe the details of protein heme interactions, we have developed a Raman difference spectroscopic technique, which allows reliable detection of very small, approximately equal to 0.01 cm-1, frequency differences. When this technique is applied to heme proteins, structural differences in the protein which perturb t...

journal_title：Biochemistry

authors： Shelnutt JA,Rousseau DL,Dethmers JK,Margoliash E

更新日期：1981-10-27 00:00:00

abstract：:Whether hydrogen bonds between side chains are energetically significant in proteins and peptides has been controversial. A method is given here for measuring these interactions in peptide helices by comparing the helix contents of peptides with 1, 2, or 3 interactions. Results are given for the glutamine--aspartate (...

journal_title：Biochemistry

authors： Huyghues-Despointes BM,Klingler TM,Baldwin RL

更新日期：1995-10-17 00:00:00

abstract：:Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...

journal_title：Biochemistry

authors： Noval MG,Esperante SA,Molina IG,Chemes LB,Prat-Gay Gd

更新日期：2016-03-15 00:00:00

abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

journal_title：Biochemistry

authors： Vedeckis WV

更新日期：1983-04-12 00:00:00

abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...

journal_title：Biochemistry

authors： Merutka G,Stellwagen E

更新日期：1989-01-10 00:00:00

abstract：:Protein kinase Calpha (PKCalpha) has been shown to contain two discrete activator sites with differing binding affinities for phorbol esters and diacylglycerols. The interaction of diacylglycerol with a low-affinity phorbol ester binding site leads to enhanced high-affinity phorbol ester binding and to a potentiated l...

journal_title：Biochemistry

authors： Slater SJ,Milano SK,Stagliano BA,Gergich KJ,Ho C,Mazurek A,Taddeo FJ,Kelly MB,Yeager MD,Stubbs CD

更新日期：1999-03-23 00:00:00

abstract：:ZFY, a male-associated Zn-finger protein encoded by the human Y chromosome, exhibits a distinctive two-finger repeat: whereas odd-numbered domains fit a general consensus, even-numbered domains exhibit systematic differences. Do these odd and even sequences encode structurally distinct surfaces for DNA recognition? As...

journal_title：Biochemistry

authors： Kochoyan M,Keutmann HT,Weiss MA

更新日期：1991-07-23 00:00:00

abstract：:Hepatic lipase (HL) hydrolysis of phosphatidylcholine (PC) was studied in recombinant high-density lipoprotein particles (r-HDL). r-HDL were made from cholate mixed micelles that contained PC, apo AI, and, in some cases, unesterified cholesterol. r-HDL were characterized using chemical composition, nondenaturing gradi...

journal_title：Biochemistry

authors： Tansey JT,Thuren TY,Jerome WG,Hantgan RR,Grant K,Waite M

更新日期：1997-10-07 00:00:00