Interaction of myotoxin a with artificial membranes: Raman spectroscopic investigation.

abstract：:One of the most intriguing questions confronting the bone morphogenetic protein family is the mechanism of ligand recognition, because there are more ligands than receptors. Crystal structures of two type II receptors, ActR-II and BMPR-II, are essentially identical, and a loop structure (A-loop) has been suggested to ...

journal_title：Biochemistry

authors： Yeh LC,Falcon WE,Garces A,Lee JC,Lee JC

更新日期：2012-09-04 00:00:00

abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...

journal_title：Biochemistry

authors： Du Vernay VH Jr,Traugh JA

更新日期：1978-05-30 00:00:00

abstract：:Duplication of a single β-strand that forms part of a β-sheet in photoactive yellow protein (PYP) was found to produce two approximately isoenergetic protein conformations, in which either the first or the second copy of the duplicated β-strand participates in the β-sheet. Whereas one conformation (big-loop) is more s...

journal_title：Biochemistry

authors： Kumar A,Nokhrin S,Woloschuk RM,Woolley GA

更新日期：2018-07-17 00:00:00

abstract：:The chirality of sulfoxidation catalyzed by two cytochrome P-450 isozymes purified from phenobarbital-induced rat liver was studied by using 4-tolyl ethyl sulfide as a substrate. Both P-450 isoenzymes, termed PB-1 and PB-4, when reconstituted with purified rat liver NADPH-cytochrome P-450 reductase and cytochrome b5, ...

journal_title：Biochemistry

authors： Waxman DJ,Light DR,Walsh C

更新日期：1982-05-11 00:00:00

abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...

journal_title：Biochemistry

authors： Coughlin RT,Haug A,McGroarty EJ

更新日期：1983-04-12 00:00:00

abstract：:The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...

journal_title：Biochemistry

authors： Sackett DL,Lippoldt RE

更新日期：1991-04-09 00:00:00

abstract：:The involvement of Cl(-) and several other monovalent anions in photosynthetic oxygen evolution was studied using photosystem II membranes depleted of Cl(-) by dialysis. The results of these studies differ significantly from results obtained using other depletion methods. Binding studies with glycerol as a cryoprotect...

journal_title：Biochemistry

authors： Olesen K,Andréasson LE

更新日期：2003-02-25 00:00:00

abstract：:With the aid of high time resolution kinetic data extracted from a flow cytometer, we determined that there are two N-formyl peptide receptor states for human neutrophils at 4 degrees C: a low affinity and a high affinity state. Competitive binding of FMLP, FNLP, and t-BOC with FNLPNTL-FL revealed different kinetic ra...

journal_title：Biochemistry

authors： Hoffman JF,Keil ML,Riccobene TA,Omann GM,Linderman JJ

更新日期：1996-10-08 00:00:00

abstract：:The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...

journal_title：Biochemistry

authors： Anas M,Sharma R,Dhamodharan V,Pradeepkumar PI,Manhas A,Srivastava K,Ahmed S,Kumar N

更新日期：2017-12-26 00:00:00

abstract：:The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...

journal_title：Biochemistry

authors： Ting LP,Wang JH

更新日期：1982-01-19 00:00:00

abstract：:The fluorescence quenching of 9-aminoacridine (9AA) in suspension of beef heart EDTA submitochondrial particles was studied and was used to calculate the pH gradient between these particles and the medium. This pH gradient, which is energy dependent, is also dependent strongly on the presence of anion species in the m...

journal_title：Biochemistry

authors： Rottenberg H,Lee CP

更新日期：1975-06-17 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...

journal_title：Biochemistry

authors： O'Brien DP,Johnson D,Byfield P,Tuddenham EG

更新日期：1992-03-17 00:00:00

abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...

journal_title：Biochemistry

authors： Zimmerman SP,Hallett RA,Bourke AM,Bear JE,Kennedy MJ,Kuhlman B

更新日期：2016-09-20 00:00:00

abstract：:A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those...

journal_title：Biochemistry

authors： Jaschke PR,Beatty JT

更新日期：2007-10-30 00:00:00

abstract：:In affinity selection, calcineurin selects from a population of randomly modified calmodulins those species with which it prefers to interact. The method shows that acetylation of lysines affects calmodulin so as to interfere with its ability to interact with calcineurin. Monoacetylation of any lysine of calmodulin re...

journal_title：Biochemistry

authors： Manalan AS,Klee CB

更新日期：1987-03-10 00:00:00

abstract：:We have found a new class of inhibitors of the bacterial bioluminescence reaction, the N,N-diphenylalkylamines and acids. We have studied the action of one of these compounds 2,2-diphenylpropylamine. The amine was competitive with the long-chain aliphatic aldehyde substrate (Ki congruent to 0.1 mM) but caused an incre...

journal_title：Biochemistry

authors： Holzman TF,Baldwin TO

更新日期：1981-09-15 00:00:00

abstract：:The divalent cation requirement for partial reactions of the catalytic turnover cycle during interfacial catalysis by pig pancreatic phospholipase A2 (PLA2) is investigated. Results show that the specific role of calcium in all the events of the catalytic cycle at the active site is not shared by other divalent cation...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1993-06-29 00:00:00

abstract：:Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...

journal_title：Biochemistry

authors： Sewell AK,Jensen LT,Erickson JC,Palmiter RD,Winge DR

更新日期：1995-04-11 00:00:00

abstract：:The retaining beta-1,4-glycanase Cex from Cellulomonas fimi, a family 10 glycosyl hydrolase, hydrolyzes xylan 40-fold more efficiently than cellulose. To gain insight into the nature of its preference for xylan, we determined the crystal structure of the Cex catalytic domain (Cex-cd) trapped as its covalent 2-deoxy-2-...

journal_title：Biochemistry

authors： Notenboom V,Birsan C,Warren RA,Withers SG,Rose DR

更新日期：1998-04-07 00:00:00

abstract：:The carboxypeptidase inhibitor from Russet Burbank potatoes (C. A. Ryan et al. (1974b), J. Biol. Chem 249, 5495) is a mixture of approximately equal amounts of two polypeptide chains containing 38 and 39 amino acid residues, respectively. The chains differ in their amino terminal sequence only, one beginning with smal...

journal_title：Biochemistry

authors： Hass GM,Nau H,Biemann K,Grahn DT,Ericsson LH,Neurath H

更新日期：1975-03-25 00:00:00

abstract：:In iron-deficient conditions, Pseudomonas aeruginosa secretes a major fluorescent siderophore named pyoverdin (Pvd), which after chelating iron(III) is transported back into the cell via its outer membrane receptor FpvA. FpvA is a TonB-dependent transport protein and has the ability to bind Pvd in its apo- or iron-loa...

journal_title：Biochemistry

authors： Clément E,Mesini PJ,Pattus F,Schalk IJ

更新日期：2004-06-22 00:00:00

abstract：:The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...

journal_title：Biochemistry

authors： Wang QW,Kline AD,Wüthrich K

更新日期：1987-10-06 00:00:00

abstract：:The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...

journal_title：Biochemistry

authors： Hsu L,Morrison M

更新日期：1985-06-18 00:00:00

abstract：:There is no complete understanding of how serine protease inhibitors of the serpin family inhibit their target enzymes. Structural and biochemical studies have suggested that serpins utilize a mechanism that is distinct from the standard mechanism of inhibition proposed for most small protein protease inhibitors. Prot...

journal_title：Biochemistry

authors： Plotnick MI,Mayne L,Schechter NM,Rubin H

更新日期：1996-06-11 00:00:00

abstract：:The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...

journal_title：Biochemistry

authors： Haunerland NH,Jacobson BL,Wesenberg G,Rayment I,Holden HM

更新日期：1994-10-18 00:00:00

abstract：:The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor α (hERα) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II β-turn conformat...

journal_title：Biochemistry

authors： Byrne C,Henen MA,Belnou M,Cantrelle FX,Kamah A,Qi H,Giustiniani J,Chambraud B,Baulieu EE,Lippens G,Landrieu I,Jacquot Y

更新日期：2016-09-27 00:00:00

abstract：:The redox properties of the [2Fe-2S] cluster in the 24 kDa subunit of bovine heart mitochondrial NADH:ubiquinone oxidoreductase (complex I) and three of its homologues have been defined using protein-film voltammetry. The clusters in all four examples display characteristic, pH-dependent redox transitions, which, unus...

journal_title：Biochemistry

authors： Zu Y,Di Bernardo S,Yagi T,Hirst J

更新日期：2002-08-06 00:00:00

abstract：:The vibrations of a water molecule in the water-oxidizing complex (WOC) of photosystem II were detected for the first time using Fourier transform infrared (FTIR) spectroscopy. In a flash-induced FTIR difference spectrum upon the S(1)-to-S(2) transition, a pair of positive and negative bands was observed at 3618 and 3...

journal_title：Biochemistry

authors： Noguchi T,Sugiura M

更新日期：2000-09-12 00:00:00

abstract：:Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...

journal_title：Biochemistry

authors： Crute JJ,Wahl AF,Bambara RA

更新日期：1986-01-14 00:00:00