Abstract:
:In iron-deficient conditions, Pseudomonas aeruginosa secretes a major fluorescent siderophore named pyoverdin (Pvd), which after chelating iron(III) is transported back into the cell via its outer membrane receptor FpvA. FpvA is a TonB-dependent transport protein and has the ability to bind Pvd in its apo- or iron-loaded form. The fluorescence properties of Pvd were used to determine the binding kinetics of metal-free and metal-loaded Pvd to FpvA and showed two major features. First, the kinetics of formation of the FpvA-Pvd complex, in vivo and in vitro, are markedly slower compared to those observed for FpvA-Pvd-metal. Second, apo-Pvd and Pvd-metal absorbed with biphasic kinetics to FpvA: the bimolecular step (association of the ligand with the receptor) is followed by a slower step (t(1/2) values of 5 and 34 min for Pvd-metal and Pvd, respectively) that presumably leads to a more stable complex. The most likely explanation for this second step is that the binding of the ligand to the receptor induces a conformational change on FpvA, which may be different, depending on the loading status of Pvd. Analysis of the dissociation of metal-free Pvd from FpvA revealed an energy and a TonB dependency. The dissociation of iron-free Pvd from FpvA in the absence of the TonB protein occurs with slow kinetics in the range of hours, but it can be highly activated by the protonmotive force and TonB to reach a kinetic with a t(1/2) of 1 min. Apparently, under iron-limited conditions, TonB activates the FpvA receptor, resulting in a fast release of iron-free Pvd and generating an unloaded FpvA receptor, competent for binding extracellular Pvd-Fe.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Clément E,Mesini PJ,Pattus F,Schalk IJdoi
10.1021/bi049768csubject
Has Abstractpub_date
2004-06-22 00:00:00pages
7954-65issue
24eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::In bacteria, two-component systems act as signaling systems to respond to environmental stimuli. Two-component systems generally consist of a sensor histidine kinase and a response regulator, which work together through histidyl-aspartyl phosphorelay to result in gene regulation. One of the two-component systems in Es...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00707
更新日期:2016-09-20 00:00:00
abstract::7,8-diaminopelargonic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA in the antepenultimate step in the biosynthesis of biotin. The wild...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026339a
更新日期:2002-10-22 00:00:00
abstract::Thermobifida fusca Cel9A-90, an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely linked to a family 3c cellulose binding domain (Cel9A-68) followed by a fibronectin III-like domain and a family 2 cellulose binding domain. To study its catalytic mechanism, 12 mutant genes wit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049394n
更新日期:2004-08-03 00:00:00
abstract::The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049797n
更新日期:2004-06-08 00:00:00
abstract::Recently, we revealed the architecture of the clamp-loader-helicase (tau-DnaB) complex in Bacillus by atomic force microscopy imaging and constructed a structural model, whereby a pentameric clamp-loader interacts with the hexameric helicase. Crucial to this model is the assumption that the clamp-loader forms a pentam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034955g
更新日期:2003-09-23 00:00:00
abstract::Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00435a014
更新日期:1989-05-02 00:00:00
abstract::For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970513r
更新日期:1997-07-08 00:00:00
abstract::The third intracellular loop (IL3) of G protein-coupled receptors (GPCRs) is an important contact domain between GPCRs and their G proteins. Previously, the IL3 of Ste2p, a Saccharomyces cerevisiae GPCR, was suggested to undergo a conformational change upon activation as detected by differential protease susceptibilit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200254h
更新日期:2011-08-16 00:00:00
abstract::The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0344073
更新日期:2003-07-01 00:00:00
abstract::Binding of the coupling ion (Na+ or Li+) and sugars to the purified melibiose permease of Escherichia coli, reconstituted in proteoliposomes, produces selective and cooperative changes of the transporter tryptophan fluorescence. To assess the individual contribution of N- or C-terminal domains of the permease to these...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961372g
更新日期:1996-09-17 00:00:00
abstract::The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400514k
更新日期:2013-07-02 00:00:00
abstract::VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0477034
更新日期:2005-04-26 00:00:00
abstract::Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00282a019
更新日期:1983-06-21 00:00:00
abstract::The phagocyte superoxide-generating NADPH oxidase, a multicomponent, membrane-bound electron transport chain, consists of cytochrome b558, p47-phox, p67-phox, and p21rac1 or p21rac2. The mechanisms of oxidase assembly are poorly understood. In previous studies using a cell-free NADPH oxidase system, we showed that pre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00175a018
更新日期:1994-03-08 00:00:00
abstract::A molecular model has been built of the equine infectious anemia virus (EIAV) proteinase on the basis of the crystal structures of the related Rous sarcoma virus (RSV) and human immunodeficiency virus (HIV) proteinases. The 104 residue long EIAV proteinase has 30 identical and 11 similar amino acids compared to those ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00064a019
更新日期:1993-04-06 00:00:00
abstract::Malondialdehyde (MDA) and nucleobase propenals can transfer oxopropenyl groups to guanine residues of DNA to yield pyrimodopurinone (M(1)G) adducts. The DNA structural requirements for reaction with alpha,beta-unsaturated aldehydes were explored. We found that single-stranded DNA is more sensitive to oxopropenylation ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0113059
更新日期:2002-04-16 00:00:00
abstract::Benzo[c]phenanthrene diol epoxide can covalently bind to the exocyclic amino group of deoxyadenosine to generate [BPh]dA adducts where the polycyclic aromatic hydrocarbon is attached to the major groove edge of DNA. This paper reports on NMR-energy minimization structural studies of the (+)-trans-anti-[BPh]dA adduct p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00097a029
更新日期:1993-11-23 00:00:00
abstract::The Vmax/Km (microM -1 min -1.) for bovine factor X activation by bovine factor IXa alpha, in the presence of sufficient [Ca2+] to saturate the initial reaction rate, was 0.007. When factor IXa beta was substituted for factor IXa alpha in this reaction, the Vmax/Km decreased to 0.001, suggesting that factor IXa alpha ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00286a007
更新日期:1983-08-16 00:00:00
abstract::The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00555a020
更新日期:1980-07-08 00:00:00
abstract::Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400691v
更新日期:2013-08-13 00:00:00
abstract::Peptides prepared from the amino termini of pro alpha 1(I) and pro alpha 1(III) collagen chains inhibit the production of pro alpha 1(I) and pro alpha 2 by rat calvaria rna in a reticulocyte cell-free system. The synthesis of other proteins was not altered, suggesting a specific effect on collagen production. Various ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00589a034
更新日期:1979-10-30 00:00:00
abstract::The solution conformation of potato carboxypeptidase inhibitor (CPI) has been investigated by 1H NMR spectroscopy. The spectrum is assigned in a sequential manner by using two-dimensional NMR techniques to identify through-bond and through-space (less than 5 A) connectivities. A set of 309 approximate interproton dist...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00398a069
更新日期:1987-12-01 00:00:00
abstract::The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952626l
更新日期:1996-03-19 00:00:00
abstract::Monomeric sarcosine oxidase (MSOX) binds the L-proline zwitterion (pKa = 10.6). The reactive substrate anion is generated by ionization of the ES complex (pKa = 8.0). Tyr317 was mutated to Phe to determine whether this step might involve proton transfer to an active site base. The mutation does not eliminate the ioniz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051898d
更新日期:2005-12-27 00:00:00
abstract::A site-specific analog of ATP, 6,6'-dithiobis (inosinyl imidodiphosphate (S2P-PNP), inactivates the ATPase activities of myosin's proteolytic fragments, heavy meromyosin (HMM) and subfragment one (SF1), by formation of mixed disulfides between the 6 position of the purine ring and certain key cysteines. The stoichiome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00680a015
更新日期:1975-05-06 00:00:00
abstract::The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900958x
更新日期:2009-09-01 00:00:00
abstract::In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000195n
更新日期:2000-07-18 00:00:00
abstract::The structure of the copper site in oxidized and reduced Rhus vernicifera stellacyanin has been studied by X-ray absorption (XAFS) spectroscopy at different pH values. Data for the oxidized protein are consistent with the fourth ligand being an O- or N-donating ligand rather than a cysteine from the disulfide bridge. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00001a026
更新日期:1995-01-10 00:00:00
abstract::Phenylcarbene and adamantylidene have been generated photochemically from the corresponding diazirines within lipid bilayers. Reasonable yields of labeled fatty acid side chains have been observed. The products have been characterized by gas chromatography-mass spectrometry and derive both from the insertion of the c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00605a026
更新日期:1978-06-13 00:00:00
abstract::Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequence...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00044a003
更新日期:1995-11-07 00:00:00