The photosystem of Rhodobacter sphaeroides assembles with zinc bacteriochlorophyll in a bchD (magnesium chelatase) mutant.

Abstract:

:A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those of the wild type. Tricine-SDS-PAGE revealed 52% light-harvesting complex alpha chain and 14% beta chain proteins compared to those of the wild type. Pigment analysis of bchD cells showed the absence of BChl and bacteriopheophytin (BPhe), but zinc bacteriochlorophyll (Zn-BChl) was discovered. Zn-BChl binds to light-harvesting 1 (LH1) and 2 (LH2) complexes in place of BChl in bchD membranes, with a LH2:LH1 ratio resembling that of wild-type cells under BChl-limiting conditions. Furthermore, the RC from the bchD mutant contained Zn-BChl in the special pair and accessory BChl binding sites, as well as carotenoid and quinone, but BPhe was absent. Comparison of the bchD mutant RC absorption spectrum to that of Acidiphilium rubrum, which contains Zn-BChl in the RC, suggests the RC protein environment at L168 contributes to A. rubrum special pair absorption characteristics rather than solely Zn-BChl. We speculate that Zn-BChl is synthesized via the normal BChl biosynthetic pathway, but with ferrochelatase supplying zinc protoporphyrin IX for enzymatic steps following the nonfunctional magnesium chelatase. The absence of BPhe in bchD cells is likely related to Zn2+ stability in the chlorin macrocycle and consequently high resistance of Zn-BChl to pheophytinization (dechelation). Possible agents prevented from dechelating Zn-BChl include the RC itself, a hypothetical dechelatase enzyme, and spontaneous processes.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Jaschke PR,Beatty JT

doi

10.1021/bi701407k

subject

Has Abstract

pub_date

2007-10-30 00:00:00

pages

12491-500

issue

43

eissn

0006-2960

issn

1520-4995

journal_volume

46

pub_type

杂志文章
  • Analysis of substrate-induced electronic, catalytic, and structural changes in inducible NO synthase.

    abstract::Inducible nitric oxide synthase (iNOS) catalyzes the NADPH-dependent formation of nitric oxide (NO) and citrulline from L-arginine and O2. In addition to serving as substrate, L-arginine alters the enzyme's heme iron spin equilibrium, increases its NADPH oxidation, and promotes assembly of active dimeric iNOS from ina...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952844e

    authors: Sennequier N,Stuehr DJ

    更新日期:1996-05-07 00:00:00

  • Structure of mammalian cytochrome P450 2C5 complexed with diclofenac at 2.1 A resolution: evidence for an induced fit model of substrate binding.

    abstract::The structure of the anti-inflammatory drug diclofenac bound in the active site of rabbit microsomal cytochrome P450 2C5/3LVdH was determined by X-ray crystallography to 2.1 A resolution. P450 2C5/3LVdH and the related enzyme 2C5dH catalyze the 4'-hydroxylation of diclofenac with apparent K(m) values of 80 and 57 micr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034556l

    authors: Wester MR,Johnson EF,Marques-Soares C,Dijols S,Dansette PM,Mansuy D,Stout CD

    更新日期:2003-08-12 00:00:00

  • Crystal structure and iron-binding properties of the R210K mutant of the N-lobe of human lactoferrin: implications for iron release from transferrins.

    abstract::Lactoferrin (Lf) and serum transferrin (Tf) combine high-affinity iron binding with an ability to release this iron at reduced pH. Lf, however, retains iron to significantly lower pH than Tf, giving the two proteins distinct functional roles. In this paper, we compared the iron-release profiles for human Lf, Tf, and t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0001224

    authors: Peterson NA,Anderson BF,Jameson GB,Tweedie JW,Baker EN

    更新日期:2000-06-06 00:00:00

  • Two upstream cysteines and the CAAX motif but not the polybasic domain are required for membrane association of Xlcaax in Xenopus oocytes.

    abstract::We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00083a022

    authors: Kloc M,Li XX,Etkin LD

    更新日期:1993-08-17 00:00:00

  • Acetylation of decarboxylated S-adenosylmethionine by mammalian cells.

    abstract::Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00350a016

    authors: Pegg AE,Wechter RS,Clark RS,Wiest L,Erwin BG

    更新日期:1986-01-28 00:00:00

  • Inhibition of DNA replication and DNA polymerase alpha activity by monoclonal anti-(DNA polymerase alpha) immunoglobulin G and F(ab) fragments.

    abstract::The effect of monoclonal anti-(DNA polymerase alpha) immunoglobulin G (IgG) and F(ab) fragments on DNA replication in lysolecithin-permeabilized human cells and on DNA polymerase alpha activity was determined. DNA polymerase alpha activity in vitro was inhibited equally by the same concentrations of monoclonal IgGs an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00346a061

    authors: Miller MR,Seighman C,Ulrich RG

    更新日期:1985-12-03 00:00:00

  • Influence of nitrogen, oxygen, and nitroimidazole radiosensitizers on DNA damage induced by ionizing radiation.

    abstract::Oxygen and nitroaromatic compounds are known to enhance the sensitivity of cells to ionizing radiation. Employing calf thymus DNA and oligo(dA)12/poly(dT), we have examined the differences to DNA damage, in particular thymine glycols and the 3'-DNA termini at strand breaks, arising from irradiation under anoxic and ox...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00060a009

    authors: Buchko GW,Weinfeld M

    更新日期:1993-03-09 00:00:00

  • Structural basis of the anionic interface preference and kcat* activation of pancreatic phospholipase A2.

    abstract::Pancreatic phospholipase A(2) (PLA2) shows a strong preference for the binding to the anionic interface and a consequent allosteric activation. In this paper, we show that virtually all the preference is mediated through 3 (Lys-53, -56, and -120) of the 12 cationic residues of bovine pancreatic PLA2. The lysine-to-met...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000740k

    authors: Yu BZ,Poi MJ,Ramagopal UA,Jain R,Ramakumar S,Berg OG,Tsai MD,Sekar K,Jain MK

    更新日期:2000-10-10 00:00:00

  • Solution structure of BmKalphaIT01, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch.

    abstract::The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi7006788

    authors: Tong X,Zhu J,Ma Y,Chen X,Wu G,He F,Cao C,Wu H

    更新日期:2007-10-09 00:00:00

  • A new chemical mechanism catalyzed by a mutated aldehyde dehydrogenase.

    abstract::NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00164a033

    authors: Corbier C,Della Seta F,Branlant G

    更新日期:1992-12-15 00:00:00

  • Porphyrin binding to quadrupled T4G4.

    abstract::We have recently reported the cation-induced self-assembly of DNA oligomers of the general sequence C4T4G4T1-4G4 into high-molecular weight multistranded structures [Marotta, S.P., Tamburri, P.A., and Sheardy, R.D. (1996) Biochemistry 35, 10484-10492]. The architecture of the proposed structure consists of a series of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi973009v

    authors: Anantha NV,Azam M,Sheardy RD

    更新日期:1998-03-03 00:00:00

  • Role of the sulfonium center in determining the ligand specificity of human s-adenosylmethionine decarboxylase.

    abstract::S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900590m

    authors: Bale S,Brooks W,Hanes JW,Mahesan AM,Guida WC,Ealick SE

    更新日期:2009-07-14 00:00:00

  • Photoaffinity labeling of oxidosqualene cyclase and squalene cyclase by a benzophenone-containing inhibitor.

    abstract::A new orally active oxidosqualene:lanosterol cyclase (OSLC) inhibitor (Ro48-8071; Morand, O. H. et al. (1997) J. Lipid Res. 38, 373-390) showed potent noncompetitive inhibition of bacterial squalene:hopene cyclase (SHC) from Alicyclobacillus acidocaldarius (IC50 = 9.0 nM, KI = 6.6 nM) and OSLC (IC50 = 40 nM, KI = 22 n...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980366c

    authors: Abe I,Zheng YF,Prestwich GD

    更新日期:1998-04-28 00:00:00

  • Insights into DNA polymerization mechanisms from structure and function analysis of HIV-1 reverse transcriptase.

    abstract::When the single-stranded RNA genome of HIV-1 is copied into double-stranded DNA, the viral enzyme reverse transcriptase (RT) catalyzes the addition of approximately 20,000 nucleotides; however, the precise mechanism of nucleotide addition is unknown. In this study, we attempt to integrate the genetic data and biochemi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00016a006

    authors: Patel PH,Jacobo-Molina A,Ding J,Tantillo C,Clark AD Jr,Raag R,Nanni RG,Hughes SH,Arnold E

    更新日期:1995-04-25 00:00:00

  • Catecholamine-stimulated guanosine 5'-O-(3-thiotriphosphate) binding to the stimulatory GTP-binding protein of adenylate cyclase: kinetic analysis in reconstituted phospholipid vesicles.

    abstract::The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with re...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00318a014

    authors: Asano T,Ross EM

    更新日期:1984-11-06 00:00:00

  • Glycolate formation catalyzed by spinach leaf transketolase utilizing the superoxide radical.

    abstract::A homogeneous preparation of transketolase was obtained from spinach leaf; the specific enzyme activity was 9.5 mumolo of glyceraldehyde-3-P formed (mg of protein)-1 min-1, when xylulose-5-P and ribose-5-P were used as the donor and acceptor, respectively, of the ketol residue. Transketolase catalyzed the formation of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00558a015

    authors: Takabe T,Asami S,Akazawa T

    更新日期:1980-08-19 00:00:00

  • A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays.

    abstract::We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that se...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi500500h

    authors: Yang Y,Zhang Q,Gao M,Yang X,Huang Z,An J

    更新日期:2014-08-05 00:00:00

  • Analysis of human red cell spectrin tetramer (head-to-head) assembly using complementary univalent peptides.

    abstract::The mass-driven assembly of spectrin dimers to form tetramers involves two equal head-to-head alpha-beta associations and requires at least 30 degrees C for interconversion to occur readily. In this paper, the properties of tetramer formation were investigated using two complementary univalent peptides (the alpha I do...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00159a030

    authors: DeSilva TM,Peng KC,Speicher KD,Speicher DW

    更新日期:1992-11-10 00:00:00

  • Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc.

    abstract::The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein canno...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00038a003

    authors: Murphy ME,Turley S,Kukimoto M,Nishiyama M,Horinouchi S,Sasaki H,Tanokura M,Adman ET

    更新日期:1995-09-26 00:00:00

  • Strand orientation in the DNA quadruplex formed from the Oxytricha telomere repeat oligonucleotide d(G4T4G4) in solution.

    abstract::The structure formed from the DNA oligonucleotide d(G4T4G4) (Oxy-1.5), which contains the Oxytricha telomere repeat T4G4, has been investigated by two-dimensional 1H and 31P NMR spectroscopy. Sequence-specific assignments have been obtained for the 1H and 31P resonances, using a combination of methods including compar...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00084a040

    authors: Smith FW,Feigon J

    更新日期:1993-08-24 00:00:00

  • Topographical localization of peroxisomal acyl-CoA ligases: differential localization of palmitoyl-CoA and lignoceroyl-CoA ligases.

    abstract::We found that peroxisomal lignoceroyl-CoA ligase, like palmitoyl-CoA ligase, is present in the peroxisomal membrane whereas the peroxisomal beta-oxidation enzyme system is localized in the matrix. To further define the role of peroxisomal acyl-CoA ligases (membrane component) in providing acyl-CoA for peroxisomal beta...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00468a027

    authors: Lazo O,Contreras M,Singh I

    更新日期:1990-04-24 00:00:00

  • Effect of lipid composition on the calcium/adenosine 5'-triphosphate coupling ratio of the Ca2+-ATPase of sarcoplasmic reticulum.

    abstract::The Ca2+-ATPase of sarcoplasmic reticulum was purified and depleted of proteolipids by solubilization in Triton X-100 and by fractionation on a DE-52 column. The protein reconstituted by deoxycholate-cholate dialysis at low lipid to protein ratios (2-5 mg of lipid/mg of protein), with either dioleoylphosphatidylethano...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00296a021

    authors: Navarro J,Toivio-Kinnucan M,Racker E

    更新日期:1984-01-03 00:00:00

  • Mechanism of cysteine desulfurase Slr0387 from Synechocystis sp. PCC 6803: kinetic analysis of cleavage of the persulfide intermediate by chemical reductants.

    abstract::Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (PLP)-dependent enzymes that cleave sulfur from cysteine via an enzyme cysteinyl persulfide intermediate. In vitro studies of these enzymes have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide intermediate, and it has been s...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi049143e

    authors: Behshad E,Parkin SE,Bollinger JM Jr

    更新日期:2004-09-28 00:00:00

  • Nuclear magnetic resonance study of the Schiff base in bacteriorhodopsin: counterion effects on the 15N shift anisotropy.

    abstract::High-resolution, solid-state 15N NMR has been used to study the chemical shift anisotropies of the Schiff bases in bacteriorhodopsin (bR) and in an extensive series of model compounds. Using slow-spinning techniques, we are able to obtain sufficient rotational sideband intensity to determine the full 15N chemical shif...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00434a033

    authors: de Groot HJ,Harbison GS,Herzfeld J,Griffin RG

    更新日期:1989-04-18 00:00:00

  • Metal-ligand vibrations of cyanoferric myeloperoxidase and cyanoferric horseradish peroxidase: evidence for a constrained heme pocket in myeloperoxidase.

    abstract::The low-frequency FeCN vibrations of cyanoferric myeloperoxidase (MPO) and horseradish peroxidase (HRP) have been measured by resonance Raman spectroscopy. The ordering of the frequencies of the predominantly FeC stretching and FeCN bending normal vibrational modes in the two peroxidases differs. These normal mode vib...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00492a012

    authors: López-Garriga JJ,Oertling WA,Kean RT,Hoogland H,Wever R,Babcock GT

    更新日期:1990-10-09 00:00:00

  • Cobalt-cytochrome c. I. Preparation, properties, and enzymic activity.

    abstract::An improved procedure for the preparation of cobalt-cytochrome c has been developed. Various factors influencing the cobalt insertion process are discussed. The optical spectra of cobalt-cytochrome c suggest a six-coordinated species. The spectral shifts occurring with oxidation-reduction are compared with those obser...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00687a003

    authors: Dickinson LC,Chien JC

    更新日期:1975-08-12 00:00:00

  • Efficiency of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli.

    abstract::We have measured the rates and efficiencies of DNA unwinding (the number of ATP molecules hydrolyzed per DNA base pair unwound) catalyzed by the RecBC,RecBCD-K177Q (a site-directed mutant in the putative ATP-binding site in the RecD subunit), and RecBCD enzymes from Escherichia coli. The DNA unwinding rate was measure...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00198a022

    authors: Korangy F,Julin DA

    更新日期:1994-08-16 00:00:00

  • Insights into the catalytic mechanisms of phenylalanine and tryptophan hydroxylase from kinetic isotope effects on aromatic hydroxylation.

    abstract::Phenylalanine hydroxylase (PheH) and tryptophan hydroxylase (TrpH) catalyze the aromatic hydroxylation of phenylalanine and tryptophan, forming tyrosine and 5-hydroxytryptophan, respectively. The reactions of PheH and TrpH have been investigated with [4-(2)H]-, [3,5-(2)H(2)]-, and (2)H(5)-phenylalanine as substrates. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0607554

    authors: Pavon JA,Fitzpatrick PF

    更新日期:2006-09-12 00:00:00

  • Structure of a mouse immunoglobulin G that lacks the entire CH1 domain: protein sequencing and small-angle X-ray scattering studies.

    abstract::The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00476a013

    authors: Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

    更新日期:1990-06-19 00:00:00

  • Stereochemical Consequences of Vinylpyruvate Hydratase-Catalyzed Reactions.

    abstract::A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b00552

    authors: Johnson WH Jr,Stack TM,Taylor SM,Burks EA,Whitman CP

    更新日期:2016-07-26 00:00:00