The amino acid sequence of a carboxypeptidase inhibitor from potatoes.

abstract：:The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...

journal_title：Biochemistry

authors： Zeghouf M,Fontecave M,Macherel D,Covès J

更新日期：1998-04-28 00:00:00

abstract：:The genomic hypervariation of human immunodeficiency virus 1 (HIV-1) could result from misincorporations by the viral reverse transcriptase. We developed an assay for reverse transcriptase fidelity during RNA-dependent as well as DNA-dependent DNA polymerization in vitro. A lacZ alpha RNA fragment transcribed by T3 RN...

journal_title：Biochemistry

authors： Ji JP,Loeb LA

更新日期：1992-02-04 00:00:00

abstract：:DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...

journal_title：Biochemistry

authors： Jorns MS,Wang B,Jordan SP

更新日期：1987-10-20 00:00:00

abstract：:3-Deoxy-d- manno-2-octulosonate-8-phosphate (KDO8P) synthase catalyzes the first step of lipopolysaccharide biosynthesis, namely condensation of phosphoenolpyruvate (PEP) with arabinose 5-phosphate (A5P), to produce KDO8P. We have characterized Campylobacter jejuni KDO8P synthase and its inhibition by KDO8P oxime. It ...

journal_title：Biochemistry

authors： Gama SR,Balachandran N,Berti PJ

更新日期：2018-09-11 00:00:00

abstract：:Using solution peptide synthesis, we have made three series of N alpha-formylated homooligopeptides, from the dipeptide to the heptapeptide, derived from L-methionine, L-norleucine, and S-methyl-L-cysteine and related to the chemotactic peptide N alpha-formylmethionylleucylphenylalanine. Compounds were prepared to det...

journal_title：Biochemistry

authors： Toniolo C,Bonora GM,Showell H,Freer RJ,Becker EL

更新日期：1984-02-14 00:00:00

abstract：:While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation of heart myofilaments occurs with binding to a single regulatory site on TnC, whereas activation of fast skeletal myofilamen...

journal_title：Biochemistry

authors： Spyracopoulos L,Li MX,Sia SK,Gagné SM,Chandra M,Solaro RJ,Sykes BD

更新日期：1997-10-07 00:00:00

abstract：:Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...

journal_title：Biochemistry

authors： Swaminathan N,Braunstein GD

更新日期：1978-12-26 00:00:00

abstract：:Terpene synthases are potentially useful biocatalysts for the synthesis of valuable compounds, such as anticancer drugs and antibiotics. The design of altered activities requires better knowledge of their mechanisms, for example, an understanding of the complex conformational changes that are part of their catalytic c...

journal_title：Biochemistry

authors： van der Kamp MW,Sirirak J,Żurek J,Allemann RK,Mulholland AJ

更新日期：2013-11-12 00:00:00

abstract：:We have studied the effect of melittin, a basic membrane-binding peptide, on Ca-ATPase activity and on protein and lipid dynamics in skeletal sarcoplasmic reticulum (SR), using time-resolved phosphorescence and fluorescence spectroscopy. Melittin completely inhibits Ca-ATPase activity, with half-maximal inhibition at ...

journal_title：Biochemistry

authors： Voss J,Birmachu W,Hussey DM,Thomas DD

更新日期：1991-07-30 00:00:00

abstract：:Exposure of mammalian cells (transformed mouse fibroblasts or rat hepatoma cells) to S-adenosyl-1,8-diamino-3-thiooctane produced profound changes in the intracellular polyamine content. Putrescine was increased and spermidine was decreased, consistent with the inhibition of spermidine synthase by this compound, which...

journal_title：Biochemistry

authors： Pegg AE,Tang KC,Coward JK

更新日期：1982-09-28 00:00:00

abstract：:Caldesmon from chicken gizzard muscle has been examined for its ability to interact with caltropin using affinity chromatography and the fluorescent probe acrylodan. The action of caltropin on the inhibitory effect of caldesmon on actomyosin ATPase was also studied. Like calmodulin, caltropin could release the inhibit...

journal_title：Biochemistry

authors： Mani RS,McCubbin WD,Kay CM

更新日期：1992-12-01 00:00:00

abstract：:Kinetics of reduction of phototrophic bacterial flavocytochromes c by exogenous flavin semiquinones and fully reduced flavins generated by laser flash photolysis have been studied. The mechanisms of reduction of Chromatium and Chlorobium flavocytochromes c are more similar to one another than previously thought. Neith...

journal_title：Biochemistry

authors： Cusanovich MA,Meyer TE,Tollin G

更新日期：1985-03-12 00:00:00

abstract：:Sequence effects on the stability of purine-motif (also called (G, A)-motif) triple helix have been investigated through two symmetry-related systems: one of them had a 5'(GGA)43' core sequence of triplex-forming oligonucleotides (TFOs), whereas the other one had a reversed 5'(AGG)43' core sequence. These (G,A)-contai...

journal_title：Biochemistry

authors： Arimondo PB,Barcelo F,Sun JS,Maurizot JC,Garestier T,Hélène C

更新日期：1998-11-24 00:00:00

abstract：:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...

journal_title：Biochemistry

authors： Kuo W,Herrick DZ,Cafiso DS

更新日期：2011-04-05 00:00:00

abstract：:UvrA is the ATPase subunit of the DNA repair enzyme (A)BC excinuclease. The amino acid sequence of this protein has revealed, in addition to two zinc fingers, three pairs of nucleotide binding motifs each consisting of a Walker A and B sequence. We have conducted site-specific mutagenesis, ATPase kinetic analyses, and...

journal_title：Biochemistry

authors： Myles GM,Hearst JE,Sancar A

更新日期：1991-04-23 00:00:00

abstract：:Our earlier NMR study showed that a two-disulfide variant of hen lysozyme containing intra-alpha-domain disulfide bridges, C6-C127 and C30-C115, is partially folded, with the alpha domain tightly folded to the nativelike conformation and the beta domain flexible or unfolded. With a view that the formation of a third d...

journal_title：Biochemistry

authors： Yokota A,Hirai K,Miyauchi H,Iimura S,Noda Y,Inoue K,Akasaka K,Tachibana H,Segawa S

更新日期：2004-06-01 00:00:00

abstract：:The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...

journal_title：Biochemistry

authors： Khouri HE,Vernet T,Ménard R,Parlati F,Laflamme P,Tessier DC,Gour-Salin B,Thomas DY,Storer AC

更新日期：1991-09-17 00:00:00

abstract：:GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of...

journal_title：Biochemistry

authors： Lefèvre JF,Fuchs RP,Daune MP

更新日期：1978-06-27 00:00:00

abstract：:Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. X-ray crystal structures of arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and urea, complete a set of struct...

journal_title：Biochemistry

authors： Cox JD,Cama E,Colleluori DM,Pethe S,Boucher JL,Mansuy D,Ash DE,Christianson DW

更新日期：2001-03-06 00:00:00

abstract：:In fosfomycin biosynthesis, the hydrolysis of cytidylyl ( S)-2-hydroxypropylphosphonate [( S)-HPP-CMP] to afford ( S)-HPP is the only uncharacterized step. Because FomD is an uncharacterized protein with a DUF402 domain that is encoded in the fosfomycin biosynthetic gene cluster, FomD was hypothesized to be responsibl...

journal_title：Biochemistry

authors： Sato S,Miyanaga A,Kim SY,Kuzuyama T,Kudo F,Eguchi T

更新日期：2018-08-14 00:00:00

abstract：:A single amino acid substitution from methionine-184 to valine (M184V) of HIV-1 reverse transcriptase (RT) evokes the 1000-fold 3TC (Lamivudine) resistance by the HIV-1 virus observed in the clinic. The M184V mutant HIV-1 RT was studied to assess its catalytic efficiency during single nucleotide incorporation using a ...

journal_title：Biochemistry

authors： Feng JY,Anderson KS

更新日期：1999-07-20 00:00:00

abstract：:Sir2 protein deacetylases (or sirtuins) catalyze NAD+-dependent conversion of epsilon-amino-acetylated lysine residues to deacetylated lysine, nicotinamide, and 2'-O-acetyl-ADP-ribose. Small-molecule modulation of sirtuin activity might treat age-associated diseases, such as type II diabetes, obesity, and neurodegener...

journal_title：Biochemistry

authors： Smith BC,Denu JM

更新日期：2007-12-18 00:00:00

abstract：:Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visua...

journal_title：Biochemistry

authors： Rathbun CM,Prescher JA

更新日期：2017-10-03 00:00:00

abstract：:Trypsin cleavage has been used to probe structure-function relationships of the Escherichia coli ATP synthase (ECF1F0). Trypsin cleaved all five subunits, alpha, beta, gamma, delta, and epsilon, in isolated ECF1. Cleavage of the alpha subunit involved the removal of the N-terminal 15 residues, the beta subunit was cle...

journal_title：Biochemistry

authors： Gavilanes-Ruiz M,Tommasino M,Capaldi RA

更新日期：1988-01-26 00:00:00

abstract：:Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...

journal_title：Biochemistry

authors： Lewis RW,Mabry J,Polisar JG,Eagen KP,Ganem B,Hess GP

更新日期：2010-06-15 00:00:00

abstract：:The influence of DNA base sequence context on the removal of a bulky benzo[a]pyrene diol epoxide-guanine adduct, (+)-trans-B[a]P-N2-dG (G*), by UvrABC nuclease from the thermophilic organism Bacillus caldotenax was investigated. The lesion was flanked by either T or C in otherwise identical complementary 43-mer duplex...

journal_title：Biochemistry

authors： Ruan Q,Liu T,Kolbanovskiy A,Liu Y,Ren J,Skorvaga M,Zou Y,Lader J,Malkani B,Amin S,Van Houten B,Geacintov NE

更新日期：2007-06-12 00:00:00

abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) has been reported to inhibit proton translocation by cytochrome bc(1) and b(6)f complexes without significantly altering the rate of electron transport, a process referred to as decoupling. To understand the possible role of DCCD in inhibiting the protonogenic reactions of cytochro...

journal_title：Biochemistry

authors： Shinkarev VP,Ugulava NB,Crofts AR,Wraight CA

更新日期：2000-12-26 00:00:00

abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...

journal_title：Biochemistry

authors： Rodriguez-Almazan C,Ruiz de Escudero I,Cantón PE,Muñoz-Garay C,Pérez C,Gill SS,Soberón M,Bravo A

更新日期：2011-01-25 00:00:00

abstract：:We have investigated the interaction of a number of synthetic 20-residue peptides, corresponding to the HA2 N-terminus of the influenza virus hemagglutinin (X31 strain), with phospholipid vesicles and monolayers. Besides the wild-type sequence, two peptides were studied with mutations corresponding to those previously...

journal_title：Biochemistry

authors： Rafalski M,Ortiz A,Rockwell A,van Ginkel LC,Lear JD,DeGrado WF,Wilschut J

更新日期：1991-10-22 00:00:00

abstract：:The peptide cyclo(hex-Amb(1)-Cys(2))-Thr(3)-Val(4)-Thr(5)-Nph(6)-NH2 was previously shown to be a slow, tight-binding inhibitor (Ki = 37 nM) of the yeast oligosaccharyl transferase (OT) [Hendrickson et al. (1996) J. Am. Chem. Soc. 118, 7636-7637]. This enzyme catalyzes the transfer of a carbohydrate moiety to an aspar...

journal_title：Biochemistry

authors： Kellenberger C,Hendrickson TL,Imperiali B

更新日期：1997-10-14 00:00:00