Abstract：
:Kinetics of reduction of phototrophic bacterial flavocytochromes c by exogenous flavin semiquinones and fully reduced flavins generated by laser flash photolysis have been studied. The mechanisms of reduction of Chromatium and Chlorobium flavocytochromes c are more similar to one another than previously thought. Neither protein is very reactive with neutral flavin semiquinones (k less than 10(7) M-1 s-1), and the reactions with fully reduced flavins are slower than expected on the basis of comparison with other electron-transfer proteins of similar redox potentials. Deazaflavin radical is reactive with the flavocytochromes c by virtue of its low redox potential, but this reaction is also slower than expected on the basis of comparison with other electron-transfer proteins. These experiments indicate that the active site for reduction of flavocytochrome c is relatively buried and probably inaccessible to solvent. Fully reduced FMN does not show an ionic strength effect in its reaction with flavocytochrome c, which demonstrates that the active site is uncharged. Sulfite, which forms an adduct with protein-bound FAD, partially blocks heme reduction. This shows that heme is reduced via the FAD. The rate constant for intramolecular electron transfer between FAD and heme must be on the order of 10(4) s-1 or larger.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Cusanovich MA,Meyer TE,Tollin Gdoi
10.1021/bi00327a002subject
Has Abstractpub_date
1985-03-12 00:00:00pages
1281-7issue
6eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Ca2+-induced fusion of phospholipid vesicles (phosphatidylcholine/phosphatidic acid, 9:1 mol/mol) prepared by ethanolic injection was followed by five different procedures: resonance energy transfer, light scattering, electron microscopy, intermixing of aqueous content, and gel filtration through Sepharose 4-B. The fi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a015
更新日期：1985-04-09 00:00:00
abstract：:The reduced hydroxylase component (MMOH) of soluble methane monooxygenase (MMO) from Methylosinus trichosporium OB3b reacts with O2 and CH4 to produce CH3OH and H2O in a single-turnover reaction. Transient kinetic analysis of this reaction has revealed at least five and probably six intermediates during the turnover [...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960596w
更新日期：1996-08-06 00:00:00
abstract：:HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the HcbA1-catalyzed reaction. Detailed mechanistic studies provide evidence for nucleophilic aromatic substitution and flavin-N5-oxide formation. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00012
更新日期：2019-03-05 00:00:00
abstract：:FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01121
更新日期：2015-12-22 00:00:00
abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011384o
更新日期：2001-12-18 00:00:00
abstract：:Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300688k
更新日期：2012-08-07 00:00:00
abstract：:alpha-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of 1-fucose from the donor sugar guanosine 5'-diphospho-beta-1-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-2H]-beta-1-fucose (GDP-[1-2H]-Fuc) as the substrate was observed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962284z
更新日期：1997-01-28 00:00:00
abstract：:Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961170k
更新日期：1996-09-17 00:00:00
abstract：:By using targeted disulfide cross-linking, we have characterized structural changes that the Escherichia coli aspartate receptor undergoes upon modification of the four specific residues that are reversibly methylated during sensory adaptation. Cysteine residues were introduced at specific positions either in the cyto...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980343y
更新日期：1998-10-20 00:00:00
abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991497w
更新日期：1999-11-23 00:00:00
abstract：:Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800164c
更新日期：2008-05-06 00:00:00
abstract：:Among the molecular strategies bacteria have set up to quickly match their transcriptional program to new environments, changes in sequence-mediated DNA curvature play a crucial role. Bacterial promoters, especially those of mesophilic bacteria, are in general preceded by a curved region. The marked thermosensitivity ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi902003g
更新日期：2010-04-06 00:00:00
abstract：:1-Aminocyclopropanephosphonate (ACPP) was synthesized, and its effects on the pyridoxal 5'-phosphate linked enzymes 1-aminocyclopropanecarboxylate (ACPC) deaminase from Pseudomonas sp. ACPC and alanine racemase from Bacillus stearothermophilus were studied. ACPP was found to be a potent inhibitor of both enzymes with ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a025
更新日期：1987-06-16 00:00:00
abstract：:Human apolipoprotein(a) kringle IV type 10 [apo(a) KIV(10)] contains a strong lysine-binding site (LBS) that mediates the interaction of Lp(a) with biological substrates such as fibrin. Mutations in the KIV(10) LBS have been reported in both the rhesus monkey and chimpanzee, and have been proposed to explain the lack ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048156p
更新日期：2005-01-18 00:00:00
abstract：:The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and beta-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain. Two mutants were crea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0479609
更新日期：2005-06-21 00:00:00
abstract：:Lipid-protein interactions in bovine rod outer segment disk membranes have been studied by using a series of eight stearic acid spin-label probes which were labeled at different carbon atom positions in the chain. In randomly oriented membrane dispersions, the electron spin resonance (ESR) spectra of the C-8, C-9, C-1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00375a005
更新日期：1987-01-13 00:00:00
abstract：:Phenylalanine acts as an allosteric activator of the tetrahydropterin-dependent enzyme phenylalanine hydroxylase. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into local conformational changes accompanying activation of rat phenylalanine hydroxylase by phenylalanine. Peptide...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1001294
更新日期：2010-04-20 00:00:00
abstract：:Paramagnetic agents produce line broadening and thus cancellation of anti phase cross-peak components in two-dimensional correlated nuclear magnetic resonance spectra. The specificity of this effect was examined to determine its utility for identifying surface residues of proteins. Ubiquitin and hen egg white lysozyme...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00495a005
更新日期：1990-10-30 00:00:00
abstract：:The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00117a033
更新日期：1992-01-21 00:00:00
abstract：:Orthophosphate and phosphate derivatives including pyrophosphate, hexametaphosphate, ATP, ADP, and inositol hexaphosphate enhance the extent of DNA degradation by iron(II) bleomycin. These phosphate-containing compounds increase both the release of free nucleic base and that of base propenals which are DNA cleavage pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00335a034
更新日期：1985-07-02 00:00:00
abstract：:HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9917535
更新日期：2000-01-18 00:00:00
abstract：:Interaction of a mitochondria-specific anionic phospholipid, cardiolipin (CL), with an intermembrane protein, cytochrome c (cyt c), yields a peroxidase complex. During apoptosis, the complex induces accumulation of CL oxidation products that are essential for detachment of cyt c from the mitochondrial membrane, induct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801507s
更新日期：2008-12-23 00:00:00
abstract：:The relationship between triacylglycerol and monoacylglycerol hydrolyzing activities of purified rat heart lipoprotein lipase was studied using emulsified trioleoylglycerol and micellar or albumin-bound monooleoylglycerol as substrates. The maximal reaction rates obtained with the two substrates were similar (650 and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00654a017
更新日期：1976-05-04 00:00:00
abstract：:Conjugative transfer of F-like plasmids in Escherichia coli is repressed by a plasmid-encoded protein, FinO. FinO blocks the translation of TraJ, a positive activator of transcription of genes required for conjugation. FinO binds a traJ antisense RNA, FinP, thereby protecting it from degradation, and catalyzes FinP-tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9911482
更新日期：1999-10-19 00:00:00
abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401218r
更新日期：2013-10-29 00:00:00
abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00096a024
更新日期：1993-11-16 00:00:00
abstract：:The role of bacterial DnaJ protein as a cochaperone of DnaK is strongly appreciated. Although DnaJ unaccompanied by DnaK can bind unfolded as well as native substrate proteins, its role as an individual chaperone remains elusive. In this study, we demonstrate that DnaJ binds a model non-native substrate with a low nan...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301543g
更新日期：2013-02-12 00:00:00
abstract：:Thymidylate synthase from phage T4 (T4TS) is part of a complex of several enzymes required for coordinate DNA synthesis in infected Escherichia coli cells. It has been proposed that similar complexes of enzymes related to DNA synthesis are also functional in eukaryotes [Pardee, A. B. (1989) Science 246, 603-608]. To d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00255a028
更新日期：1994-12-27 00:00:00
abstract：:N-(1-Pyrenyl)iodoacetamide has been used to introduce fluorescent probes into Escherichia coli RNA polymerase. After an incubation time of 15 min, approximately 2 pyrene equiv was introduced per enzyme molecule. There was no further increase in modification after more extended periods of incubation. Neither calf thymu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00215a028
更新日期：1991-01-08 00:00:00
abstract：:Human serum albumin (HSA) is the most abundant protein in the blood plasma and is involved in the transport of metal ions. Four metal-binding sites with different specificities have been described in HSA: (i) the N-terminal site provided by Asp1, Ala2, and His3, (ii) the site at the reduced Cys34, (iii) site A, includ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061783p
更新日期：2007-02-27 00:00:00
