Conformational changes in the cytoplasmic domain of the Escherichia coli aspartate receptor upon adaptive methylation.

abstract：:Modification of pig kidney fructose-1,6-bisphosphatase with 2,3-butanedione in borate buffer (pH 7.8) leads to the loss of the activation of the enzyme by monovalent cations, as well as to the loss of allosteric adenosine 5'-monophosphate (AMP) inhibition. In agreement with the results obtained for the butanedione mod...

journal_title：Biochemistry

authors： Marcus F

更新日期：1976-08-10 00:00:00

abstract：:The interaction of cholesterol with phospholipids has been studied with a variety of techniques; however, the possible consequences of such interactions in vivo have not been demonstrated. In this study, the cholesterol-dependent absorbance spectrum of cytochrome P-450scc was used to monitor cholesterol availability i...

journal_title：Biochemistry

authors： Stevens VL,Lambeth JD,Merrill AH Jr

更新日期：1986-07-29 00:00:00

abstract：:Membrane vesicles isolated from Escherichia coli ML 308--225 have been analyzed by crossed immunoelectrophoresis, and immunoprecipitates corresponding to the following cellular components have been identified: ATPase (EC 3.6.1,3), two or three NADH dehydrogenases (EC 1.6.99.3), D-lactate dehydrogenase (EC 1.1.1.27), g...

journal_title：Biochemistry

authors： Owen P,Kaback HR

更新日期：1979-04-17 00:00:00

abstract：:Neuraminidase is one of the two glycoprotein spikes protruding from the influenza virus membrane. We have determined by X-ray crystallography the native structure of B/Lee/40 neuraminidase (NA) and the structures of its crystals soaked with a substrate, N-acetylneuraminyllactose (NANL), and an inhibitor, 2-deoxy-2,3-d...

journal_title：Biochemistry

authors： Janakiraman MN,White CL,Laver WG,Air GM,Luo M

更新日期：1994-07-12 00:00:00

abstract：:To elucidate the mechanism of solute diffusion through lipid bilayer membranes, nearly 4 ns of molecular dynamics simulations of solutes in phospholipid bilayers was conducted. The study, the first atomic level study of solute diffusion in a lipid bilayer, involved four simulations of an all-atom representation of a f...

journal_title：Biochemistry

authors： Bassolino-Klimas D,Alper HE,Stouch TR

更新日期：1993-11-30 00:00:00

abstract：:We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a ...

journal_title：Biochemistry

authors： Epand RM,Epand RF,Martin I,Ruysschaert JM

更新日期：2001-07-31 00:00:00

abstract：:Seven mutant LH1 polypeptides of Rhodobactor sphaeroides have been isolated, and their behaviors in in vitro reconstitution of LH1 and its subunit complex have been characterized. Two mutants were selected to address the increased stability of the subunit complex of Rb. sphaeroides compared with that of Rhodobacter ca...

journal_title：Biochemistry

authors： Davis CM,Bustamante PL,Todd JB,Parkes-Loach PS,McGlynn P,Olsen JD,McMaster L,Hunter CN,Loach PA

更新日期：1997-03-25 00:00:00

abstract：:The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...

journal_title：Biochemistry

authors： Hauser H,Pascher I,Sundell S

更新日期：1988-12-27 00:00:00

abstract：:Deazaflavins have been found to act as potent catalysts in the photoreduction of flavoproteins in the presence of EDTA and other "photosubstrates". In distinction to the catalysis brought about by normal flavins which involves dark reaction of the photoreduced flavin catalyst, the mechanism of the catalysis by deazafl...

journal_title：Biochemistry

authors： Massey V,Hemmerich P

更新日期：1978-01-10 00:00:00

abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1984-12-04 00:00:00

abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

journal_title：Biochemistry

authors： Glick MC

更新日期：1979-06-12 00:00:00

abstract：:The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...

journal_title：Biochemistry

authors： Hémadi M,Miquel G,Kahn PH,El Hage Chahine JM

更新日期：2003-03-18 00:00:00

abstract：:The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...

journal_title：Biochemistry

authors： Vanderkooi JM,Ierokomas A,Nakamura H,Martonosi A

更新日期：1977-04-05 00:00:00

abstract：:Lon is a homo-oligomeric ATP-dependent serine protease that functions in the degradation of damaged and certain regulatory proteins. This enzyme has emerged as a novel target in the development of antibiotics because of its importance in conferring bacterial virulence. In this study, we explored the mechanism by which...

journal_title：Biochemistry

authors： Frase H,Lee I

更新日期：2007-06-05 00:00:00

abstract：:The thermal unfolding of rabbit skeletal heavy meromyosin (HMM), myosin subfragment 1, and subfragment 2 has been studied by differential scanning calorimetry (DSC). Two distinct endotherms are observed in the DSC scan of heavy meromyosin. The first endotherm, with a Tm of 41 degrees C at pH 7.9 in 0.1 M KCl, is assig...

journal_title：Biochemistry

authors： Shriver JW,Kamath U

更新日期：1990-03-13 00:00:00

abstract：:Three mutants, Asp55-Gly, Tyr306-->Phe, and Asp309-->Asn, of Aspergillus awamori glucoamylase (identical to Aspergillus niger glucoamylase) were constructed to elucidate the roles of two conserved regions within fungal glucoamylases. Kinetic studies indicate that both of these regions are closely associated with activ...

journal_title：Biochemistry

authors： Sierks MR,Svensson B

更新日期：1993-02-02 00:00:00

abstract：:P1,P2-Bidentate Co(NH3)4PP was found to be a competitive inhibitor of pyrophosphatase vs. MgPP (Kis = 8.7 mM, pH 7) and, in the presence of Mg2+, an active substrate as well. P1,P2-Bidentate Cr(III) complexes of pyrophosphate, imidodiphosphate, and methylenediphosphonate were also competitive inhibitors vs. MgPP (pH 5...

journal_title：Biochemistry

authors： Knight WB,Fitts SW,Dunaway-Mariano D

更新日期：1981-07-07 00:00:00

abstract：:Alpha-synuclein modulates dopamine homeostasis in dopamine-producing neurons of substantia nigra, partly through regulation of human dopamine transporter (hDAT) activity. To identify the underlying mechanisms, we disrupted the modulation of hDAT activity by wild-type (wt) alpha-synuclein, and its familial Parkinson's ...

journal_title：Biochemistry

authors： Wersinger C,Vernier P,Sidhu A

更新日期：2004-02-10 00:00:00

abstract：:Reducing sugars such as glucose react nonenzymatically with protein amino groups to initiate a posttranslational modification process known as advanced glycosylation. Nucleotide bases also participate in advanced glycosylation reactions, producing DNA-linked advanced glycosylation endproducts (AGEs) that cause mutatio...

journal_title：Biochemistry

authors： Papoulis A,al-Abed Y,Bucala R

更新日期：1995-01-17 00:00:00

abstract：:Kinetics studies in homogeneous aqueous solution showed that solubilized penicillin-binding protein 2a (sPBP2a) of methicillin-resistant Staphylococcus aureus (a bacterial DD-peptidase) was inhibited by the amphiphilic glycolipid antibiotic moenomycin. Inhibition at the peptidase site was determined by competition exp...

journal_title：Biochemistry

authors： Graves-Woodward K,Pratt RF

更新日期：1999-08-10 00:00:00

abstract：:The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further...

journal_title：Biochemistry

authors： Koch N,Hämmerling GJ

更新日期：1985-10-22 00:00:00

abstract：:The relationship between steroid binding and protein subunit interactions of rabbit sex steroid binding protein (rSBP) has been studied by steady-state and time-resolved fluorescence spectroscopy. The high-affinity (Ka approximately 10(8) M-1 at 4 degrees C), fluorescent estrogen d-1,3,5(10),6,8-estrapentaene-3,17 bet...

journal_title：Biochemistry

authors： Casali E,Petra PH,Ross JB

更新日期：1990-10-09 00:00:00

abstract：:The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on...

journal_title：Biochemistry

authors： Labuda D,Pörschke D

更新日期：1980-08-05 00:00:00

abstract：:In the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), the genome sequencing revealed the presence of three operons encoding formate dehydrogenases. fdh1 encodes an alphabetagamma trimeric enzyme containing 11 heme binding sites; fdh2 corresponds to an alphabetagamma trimeric enzyme wi...

journal_title：Biochemistry

authors： ElAntak L,Dolla A,Durand MC,Bianco P,Guerlesquin F

更新日期：2005-11-15 00:00:00

abstract：:The relationship between the domain structure of the alpha subunit of Escherichia coli tryptophan synthase and the mechanism of unfolding of the alpha subunit is investigated. Previous studies of the unfolding of the alpha subunit by increasing concentrations of guanidine hydrochloride or urea detected a partially unf...

journal_title：Biochemistry

authors： Miles EW,Yutani K,Ogasahara K

更新日期：1982-05-25 00:00:00

abstract：:The SecA molecular nanomachine in bacteria uses energy from ATP hydrolysis to drive post-translational secretion of preproteins through the SecYEG translocon. Cytosolic SecA exists in a dimeric, "closed" state with relatively low ATPase activity. After binding to the translocon, SecA undergoes major conformational rea...

journal_title：Biochemistry

authors： Maki JL,Krishnan B,Gierasch LM

更新日期：2012-02-21 00:00:00

abstract：:Nicotinic acetylcholine receptors (nAChRs) were studied in detail in the past regarding their interaction with therapeutic and drug addiction related compounds. Using fast kinetic whole-cell recording, we have now studied effects of tacrine, an agent used clinically to treat Alzheimer's disease, on currents elicited b...

journal_title：Biochemistry

authors： Cheffer A,Ulrich H

更新日期：2011-03-22 00:00:00

abstract：:A new method is described for determining molecular structures from NMR data. The approach utilizes 2D NOESY back-calculations to generate simulated spectra for structures obtained from distance geometry (DG) computations. Comparison of experimental and back-calculated spectra, including analysis of cross-peak buildup...

journal_title：Biochemistry

authors： Summers MF,South TL,Kim B,Hare DR

更新日期：1990-01-16 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) catalyzes the acetyl-CoA-dependent acetylation of chloramphenicol by a ternary complex mechanism with a rapid equilibrium and essentially random order of addition of substrates. Such a kinetic mechanism for a two-substrate reaction provides an opportunity to compare the affinity...

journal_title：Biochemistry

authors： Ellis J,Bagshaw CR,Shaw WV

更新日期：1991-11-05 00:00:00

abstract：:Histone deacetylases have proven to be attractive novel targets for the treatment of cancer. The first inhibitor of histone deacetylases was approved for the treatment of cutaneous T-cell lymphoma in 2006. The identification of new lead structures with improved effectiveness and fewer side effects is necessary. This r...

journal_title：Biochemistry

authors： Sykora J,Meyer-Almes FJ

更新日期：2010-02-23 00:00:00