Abstract：
:Deazaflavins have been found to act as potent catalysts in the photoreduction of flavoproteins in the presence of EDTA and other "photosubstrates". In distinction to the catalysis brought about by normal flavins which involves dark reaction of the photoreduced flavin catalyst, the mechanism of the catalysis by deazaflavins has been shown to involve unstable, strongly reducing radicals which are generated by photolysis of a preformed covalent dimer. By this new method it is possible to reduce not only flavoproteins but a variety of other redox proteins, including heme proteins and iron-sulfur proteins. By virtue of its great catalytic efficiency, it is possible to employ concentrations of deazaflavin sufficiently low as not to interfere with the spectral evaluation of the reduced proteins obtained.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Massey V,Hemmerich Pdoi
10.1021/bi00594a002subject
Has Abstractpub_date
1978-01-10 00:00:00pages
9-16issue
1eissn
0006-2960issn
1520-4995journal_volume
17pub_type
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