Activated leukocytes oxidatively damage DNA, RNA, and the nucleotide pool through halide-dependent formation of hydroxyl radical.

abstract：:This study highlights the effect of incorporation of locked nucleic acid (LNA) on improving the functional efficacy of DNAzymes against microRNAs (antagomirzymes). DNAzymes were designed against two different sites of miR-27a, which were encompassed both within the precursor and mature form of miRNA. The cleavage and ...

journal_title：Biochemistry

authors： Kaur H,Scaria V,Maiti S

更新日期：2010-11-09 00:00:00

abstract：:Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...

journal_title：Biochemistry

authors： Barranco-Medina S,Kakorin S,Lázaro JJ,Dietz KJ

更新日期：2008-07-08 00:00:00

abstract：:We have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, K562, KG1, and HL-60, which were metabolically labeled with [14C]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. Individual neutral gl...

journal_title：Biochemistry

authors： Buehler J,Qwan E,DeGregorio MW,Macher BA

更新日期：1985-11-19 00:00:00

abstract：:Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleoside 5'-diphosphates (NDPs) to deoxynucleotides (dNDPs). The active site for NDP reduction resides in α2, and the essential diferric-tyrosyl radical (Y(122)(•)) cofactor that initiates transfer of the radical to the act...

journal_title：Biochemistry

authors： Seyedsayamdost MR,Yee CS,Stubbe J

更新日期：2011-03-01 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase, EC 1.1.2.3) are connected by a typical hinge peptide. To probe the importance of the structural integrity of the hinge region for efficient intraprotein electron transfer, three mutant enzymes have been constructed: H delta 3 [Sharp...

journal_title：Biochemistry

authors： Sharp RE,Chapman SK,Reid GA

更新日期：1996-01-23 00:00:00

abstract：:Aromatic amine dehydrogenase (AADH) is the second enzyme known to possess the tryptophan tryptophylquinone (TTQ) prosthetic group. Its ability to catalyze the oxidative deamination of a wide range of aromatic and aliphatic amines has been investigated. Steady-state and transient kinetic studies of the reaction of AADH...

journal_title：Biochemistry

authors： Hyun YL,Davidson VL

更新日期：1995-01-24 00:00:00

abstract：:Extensive studies of the physiological protein-protein electron-transfer (ET) complex between yeast cytochrome c peroxidase (CcP) and cytochrome c (Cc) have left unresolved questions about how formation and dissociation of binary and ternary complexes influence ET. We probe this issue through a study of the photocycle...

journal_title：Biochemistry

authors： Page TR,Hoffman BM

更新日期：2015-02-10 00:00:00

abstract：:The physiological roles of immunophilins are unclear, but many possess peptidyl-prolyl isomerase (PPIase) activity, and they have been found in all organisms examined to date, implying that they are involved in fundamental, protein-folding processes. The chloroplast thylakoid lumen of the higher plant Arabidopsis thal...

journal_title：Biochemistry

authors： Edvardsson A,Shapiguzov A,Petersson UA,Schröder WP,Vener AV

更新日期：2007-08-21 00:00:00

abstract：:The formation of ternary complexes involving serine proteases, borate, and an alcohol has important implications for understanding the physiological actions of borate and for the development of tight binding inhibitors for this class of enzymes. Recent studies of a related enzyme, gamma-glutamyl transpeptidase, which ...

journal_title：Biochemistry

authors： London RE,Gabel SA

更新日期：2002-05-14 00:00:00

abstract：:Treatment of the beta 2 adrenergic receptor with the reducing agent dithiothreitol (DTT) is known to abolish ligand binding to the receptor. Interestingly, the loss of binding can be prevented by preoccupation of the receptor with ligand. It is unclear, however, whether the ligand blocks access of DTT to the receptor,...

journal_title：Biochemistry

authors： Lin S,Gether U,Kobilka BK

更新日期：1996-11-19 00:00:00

abstract：:Using the thrombin-cut [68-30 kilodalton (kDa)] myosin subfragment 1 (S-1) whose heavy chain has been selectively split within the central 50-kDa region, at Lys-560, with concomitant specific alterations of the ATPase and actin binding properties [Chaussepied, P., Mornet, D., Audemard, E., Derancourt, J., & Kassab, R....

journal_title：Biochemistry

authors： Chaussepied P,Mornet D,Kassab R

更新日期：1986-10-21 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...

journal_title：Biochemistry

authors： Li J,Tsai MD

更新日期：2002-03-26 00:00:00

abstract：:Endonuclease VIII (Nei) is one of three enzymes in Escherichia coli that are involved in base-excision repair of oxidative damage to DNA. We investigated the substrate specificity and excision kinetics of this DNA glycosylase for bases in DNA that have been damaged by free radicals. Two different DNA substrates were p...

journal_title：Biochemistry

authors： Dizdaroglu M,Burgess SM,Jaruga P,Hazra TK,Rodriguez H,Lloyd RS

更新日期：2001-10-09 00:00:00

abstract：:The catalytic activity and inhibitor binding energetics of enzymes are often pH-dependent properties. Aspartic proteases comprise an important class of enzyme targets for structure-based drug design. We have performed a complete thermodynamic study of pepstatin binding to plasmepsin II, an aspartic proteinase found in...

journal_title：Biochemistry

authors： Xie D,Gulnik S,Collins L,Gustchina E,Suvorov L,Erickson JW

更新日期：1997-12-23 00:00:00

abstract：:Cytochromes c require covalent attachment of heme via two thioether bonds at conserved CXXCH motifs, a process accomplished in prokaryotes by eight integral membrane proteins (CcmABCDEFGH), termed System I. Heme is trafficked from inside the cell to outside (via CcmABCD) and chaperoned (holoCcmE) to the cytochrome c s...

journal_title：Biochemistry

authors： Sutherland MC,Rankin JA,Kranz RG

更新日期：2016-06-07 00:00:00

abstract：:Triclinic crystals of hen egg-white lysozyme cross-linked with glutaraldehyde have been treated with various denaturants and found to be susceptible to x-ray structure analysis even after major conformational changes in the protein. Cross-linked crystals were isomorphous with the native form, and electron density diff...

journal_title：Biochemistry

authors： Yonath A,Sielecki A,Moult J,Podjarny A,Traub W

更新日期：1977-04-05 00:00:00

abstract：:Mixed phospholipid systems of ether-linked 1,2-dihexadecylphosphatidylcholine (DHPC) and ester-linked 1,2-dipalmitoylphosphatidylcholine (DPPC) have been studied by differential scanning calorimetry and X-ray diffraction. At maximum hydration (60 wt % water), DHPC shows three reversible transitions: a main (chain melt...

journal_title：Biochemistry

authors： Kim JT,Mattai J,Shipley GG

更新日期：1987-10-20 00:00:00

abstract：:Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...

journal_title：Biochemistry

authors： Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

更新日期：1995-09-12 00:00:00

abstract：:Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O(6)-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to increased protein activity but also to higher stability, especially...

journal_title：Biochemistry

authors： Mollwitz B,Brunk E,Schmitt S,Pojer F,Bannwarth M,Schiltz M,Rothlisberger U,Johnsson K

更新日期：2012-02-07 00:00:00

abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...

journal_title：Biochemistry

authors： Bornarth CJ,Ranalli TA,Henricksen LA,Wahl AF,Bambara RA

更新日期：1999-10-05 00:00:00

abstract：:Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes the transfer of the amino group of L-histidinol phosphate (Hsp) to 2-oxoglutarate to form imidazole acetol phosphate (IAP) and glutamate. Thus, HspAT recognizes two kinds of substrates, Hsp and glutamat...

journal_title：Biochemistry

authors： Haruyama K,Nakai T,Miyahara I,Hirotsu K,Mizuguchi H,Hayashi H,Kagamiyama H

更新日期：2001-04-17 00:00:00

abstract：:Pyridoxal 5'-triphospho-5'-adenosine (AP3-PL), the affinity labeling reagent specific for lysine residues in the nucleotide-binding site of several enzymes [Tagaya, M., & Fukui, T. (1986) Biochemistry 25, 2958-2964; Yagami, T., Tagaya, M., & Fukui, T. (1988) FEBS Lett. 229, 261-264], was used to identify the ATP-bindi...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Fukui T,Tagaya M,Blanquet S

更新日期：1990-12-25 00:00:00

abstract：:An NMR study has been made of porcine pancreatic phospholipase A2 (PLA) in three environments: free in solution, in a binary complex with dodecylphosphocholine micelles, and in a ternary complex with a micelle and the substrate-like inhibitor (R)-1-octyl-2-(N-dodecanoylamino)-2-deoxyglycero-3-phosph oglycol. 1H and 15...

journal_title：Biochemistry

authors： Peters AR,Dekker N,van den Berg L,Boelens R,Kaptein R,Slotboom AJ,de Haas GH

更新日期：1992-10-20 00:00:00

abstract：:Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr(5)UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands. Thus, the ACC-trinucleotide motif, previously identified as important for stabilizing the junction, is now extended to...

journal_title：Biochemistry

authors： Hays FA,Vargason JM,Ho PS

更新日期：2003-08-19 00:00:00

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:The functional role of the Ca (2+) ion in the oxygen-evolving complex of photosystem II is not yet clear. Current models explain why the redox cycle of the complex would be interrupted after the S 3 state without Ca (2+), but the literature shows that it is interrupted after the S 2 state. Reinterpretation of the lite...

journal_title：Biochemistry

authors： Miqyass M,Marosvölgyi MA,Nagel Z,Yocum CF,van Gorkom HJ

更新日期：2008-07-29 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...

journal_title：Biochemistry

authors： Kirpich JS,Chang CW,Franse J,Yu Q,Escobar FV,Jenkins AJ,Martin SS,Narikawa R,Ames JB,Lagarias JC,Larsen DS

更新日期：2021-01-13 00:00:00

abstract：:The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100 degrees C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and 1H NMR, the...

journal_title：Biochemistry

authors： Turco SJ,Hull SR,Orlandi PA Jr,Shepherd SD,Homans SW,Dwek RA,Rademacher TW

更新日期：1987-09-22 00:00:00