当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Thermodynamics of the dimer-decamer transition of reduced human and plant 2-cys peroxiredoxin.

Thermodynamics of the dimer-decamer transition of reduced human and plant 2-cys peroxiredoxin.

Abstract：

:Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine peroxoredoxins from Homo sapiens, Arabidopsis thaliana, and Pisum sativum as well as a carboxy-terminally truncated variant were subjected to ITC analysis by stepwise injection into the reaction vessel under various redox conditions. The direct measurement of the decamer-dimer equilibrium of reduced peroxiredoxin revealed a critical concentration in the very low micromolar range. The data suggest a cooperative assembly above this critical transition concentration where a nucleus facilitates assembly. The rather abrupt transition indicates that assembly processes do not occur below the critical transition concentration while oligomerization is efficiently triggered above it. The magnitude of the measured enthalpy confirmed the endothermic nature of the peroxiredoxin oligomerization. Heterocomplexes between peroxiredoxin polypeptides from different species were not formed. We conclude that a functional constraint conserved the dimer-decamer transition with highly similar critical transition concentrations despite emerging sequence variation during evolution.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Barranco-Medina S,Kakorin S,Lázaro JJ,Dietz KJ

doi

10.1021/bi8002956

subject

Has Abstract

pub_date

2008-07-08 00:00:00

pages

7196-204

issue

27

eissn

0006-2960

issn

1520-4995

journal_volume

47

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Thermodynamics of glycophorin in phospholipid bilayer membranes.

    abstract：:We have developed a model of glycophorin in a phospholipid bilayer membrane in order to study the thermodynamics of this system and to understand the detailed behavior of recent calorimetric data. We assume that the larger glycophorin polar group can be considered as either adopting a pancakelike conformation at the b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00381a019

    authors： MacDonald AL,Pink DA

    更新日期：1987-04-07 00:00:00

  • Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis.

    abstract：:Guided by the X-ray structure analysis of a crystalline EcoRV-d(GGGATATCCC) complex (Winkler, in preparation), we have begun to identify functionally important amino acid residues of EcoRV. We show here that Asn70, Asp74, Ser183, Asn185, Thr186, and Asn188 are most likely involved in the binding and/or cleavage of the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00240a011

    authors： Thielking V,Selent U,Köhler E,Wolfes H,Pieper U,Geiger R,Urbanke C,Winkler FK,Pingoud A

    更新日期：1991-07-02 00:00:00

  • 13C NMR studies of methylene and methine carbons of substrate bound to a 280,000-dalton protein, porphobilinogen synthase.

    abstract：:13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00412a039

    authors： Jaffe EK,Markham GD

    更新日期：1988-06-14 00:00:00

  • Fluorescence energy transfer measurements of spatial relationships between sulfhydryl groups of thiolase I from porcine heart.

    abstract：:Mitochondrial thiolase I from pig heart has been found to have at least two and possibly three reactive sulfhydryl residues at or near the active site [Izbicka-Dimitrijević, E., & Gilbert, H. F. (1982) Biochemistry 21, 6112-6118; Izbicka-Dimitrijević, E., & Gilbert, H. F. (1984) Biochemistry 23, 4318-4324]. In the nat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00321a015

    authors： Izbicka E,Gilbert HF

    更新日期：1984-12-18 00:00:00

  • Biochemical and Functional Evaluation of the Intramolecular Disulfide Bonds in the Zinc-Chelating Antimicrobial Protein Human S100A7 (Psoriasin).

    abstract：:Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00781

    authors： Cunden LS,Brophy MB,Rodriguez GE,Flaxman HA,Nolan EM

    更新日期：2017-10-31 00:00:00

  • Mechanism of substrate inactivation of Escherichia coli S-adenosylmethionine decarboxylase.

    abstract：:S-Adenosylmethionine decarboxylase, a pyruvoyl-containing decarboxylase, is inactivated in a time-dependent process under turnover conditions. The inactivation is dependent on the presence of both substrate and Mg2+, which is also required for enzyme activity. The rate of inactivation is dependent on the concentration...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00394a022

    authors： Anton DL,Kutny R

    更新日期：1987-10-06 00:00:00

  • An attempt to discriminate catalytic and regulatory proton binding sites in membrane-bound, thiol-reduced chloroplast ATPase.

    abstract：:The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00132a013

    authors： Valerio M,de Kouchkovsky Y,Haraux F

    更新日期：1992-05-05 00:00:00

  • Parallel symmetric immobile DNA junctions as substrates for E. coli RuvC Holliday junction resolvase.

    abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020319r

    authors： Sha R,Liu F,Iwasaki H,Seeman NC

    更新日期：2002-09-10 00:00:00

  • Structural studies of the contractile tail sheath protein of bacteriophage T4. 2. Structural analyses of the tail sheath protein, Gp18, by limited proteolysis, immunoblotting, and immunoelectron microscopy.

    abstract：:The molecular structure of the T4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. Gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00473a009

    authors： Arisaka F,Takeda S,Funane K,Nishijima N,Ishii S

    更新日期：1990-05-29 00:00:00

  • Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization.

    abstract：:The purL gene of Escherichia coli encoding the enzyme formylglycinamidine ribonucleotide (FGAM) synthetase which catalyzes the conversion of formylglycinamide ribonucleotide (FGAR), glutamine, and MgATP to FGAM, glutamate, ADP, and Pi has been cloned and sequenced. The mature protein, as deduced by the structural gene...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00432a017

    authors： Schendel FJ,Mueller E,Stubbe J,Shiau A,Smith JM

    更新日期：1989-03-21 00:00:00

  • High-potential iron-sulfur protein (HiPIP) is the major electron donor to the reaction center complex in photosynthetically growing cells of the purple bacterium Rubrivivax gelatinosus.

    abstract：:A gene encoding the high-potential iron-sulfur protein (HiPIP) was cloned from the purple photosynthetic bacterium Rubrivivax gelatinosus. An insertional disruption of this gene by a kanamycin resistance cartridge resulted in a significant decrease in the growth rate under photosynthetic growth conditions. Flash-induc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026511a

    authors： Nagashima KV,Matsuura K,Shimada K,Verméglio A

    更新日期：2002-11-26 00:00:00

  • Bleaching kinetics of artificial visual pigments with modifications near the ring-polyene chain connection.

    abstract：:Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011461f

    authors： Szundi I,de Lera AR,Pazos Y,Alvarez R,Oliana M,Sheves M,Lewis JW,Kliger DS

    更新日期：2002-02-12 00:00:00

  • Threonine 41 in beta-catenin serves as a key phosphorylation relay residue in beta-catenin degradation.

    abstract：:Beta-catenin phosphorylation at serine 45 (Ser45), threonine 41 (Thr41), Ser37, and Ser33 is critical for beta-catenin degradation, and regulation of beta-catenin phosphorylation is a central part of the canonical Wnt signaling pathway. Beta-catenin mutations at Ser45, Thr41, Ser37, and Ser33 perturb beta-catenin degr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0601149

    authors： Wu G,He X

    更新日期：2006-04-25 00:00:00

  • Kinetic studies of Thermobifida fusca Cel9A active site mutant enzymes.

    abstract：:Thermobifida fusca Cel9A-90, an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely linked to a family 3c cellulose binding domain (Cel9A-68) followed by a fibronectin III-like domain and a family 2 cellulose binding domain. To study its catalytic mechanism, 12 mutant genes wit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049394n

    authors： Zhou W,Irwin DC,Escovar-Kousen J,Wilson DB

    更新日期：2004-08-03 00:00:00

  • Structure of a Novel Phosphotriesterase from Sphingobium sp. TCM1: A Familiar Binuclear Metal Center Embedded in a Seven-Bladed β-Propeller Protein Fold.

    abstract：:A novel phosphotriesterase was recently discovered and purified from Sphingobium sp. TCM1 (Sb-PTE) and shown to catalyze the hydrolysis of a broad spectrum of organophosphate esters with a catalytic efficiency that exceeds 10(6) M(-1) s(-1) for the hydrolysis of triphenyl phosphate. The enzyme was crystallized and the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00364

    authors： Mabanglo MF,Xiang DF,Bigley AN,Raushel FM

    更新日期：2016-07-19 00:00:00

  • Morin inhibits the early stages of amyloid β-peptide aggregation by altering tertiary and quaternary interactions to produce "off-pathway" structures.

    abstract：:Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300113x

    authors： Lemkul JA,Bevan DR

    更新日期：2012-07-31 00:00:00

  • Fetal Alz-50 clone 1 interacts with the human orthologue of the Kelch-like Ech-associated protein.

    abstract：:The fetal Alz-50 reactive clone 1 (FAC1) protein exhibits altered expression and subcellular localization during neuronal development and neurodegenerative diseases such as Alzheimer's disease. Using the yeast two-hybrid screen, the human orthologue of Keap1 (hKeap1) was identified as a FAC1 interacting protein. Keap1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0494166

    authors： Strachan GD,Morgan KL,Otis LL,Caltagarone J,Gittis A,Bowser R,Jordan-Sciutto KL

    更新日期：2004-09-28 00:00:00

  • Helix 8 and helix 10 are involved in substrate recognition in the rat monocarboxylate transporter MCT1.

    abstract：:Transport of lactate, pyruvate, and the ketone bodies, acetoacetate and beta-hydroxybutyrate, is mediated in many mammalian cells by the monocarboxylate transporter MCT1. To be accepted as a substrate, a carboxyl group and an unpolar side chain are necessary. Site-directed mutagenesis of the rat MCT1 was used to ident...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990973f

    authors： Rahman B,Schneider HP,Bröer A,Deitmer JW,Bröer S

    更新日期：1999-08-31 00:00:00

  • Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein.

    abstract：:Multidrug resistance protein (MRP) and P-glycoprotein (Pgp) are both members of the superfamily of ATP binding cassette plasma membrane drug transport proteins, which may be partly responsible for multidrug resistance of tumor cells. Although MRP has been identified as an organic anion transporter and Pgp as a transpo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9718043

    authors： Essodaigui M,Broxterman HJ,Garnier-Suillerot A

    更新日期：1998-02-24 00:00:00

  • NMR and computational characterization of mitomycin cross-linked to adjacent deoxyguanosines in the minor groove of the d(T-A-C-G-T-A).d(T-A-C-G-T-A) duplex.

    abstract：:Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00463a032

    authors： Norman D,Live D,Sastry M,Lipman R,Hingerty BE,Tomasz M,Broyde S,Patel DJ

    更新日期：1990-03-20 00:00:00

  • Biochemical Characterization of Yeast Xrn1.

    abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b01035

    authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

    更新日期：2020-04-21 00:00:00

  • Probing the specificity determinants of amino acid recognition by arginase.

    abstract：:Arginase is a binuclear manganese metalloenzyme that serves as a therapeutic target for the treatment of asthma, erectile dysfunction, and atherosclerosis. In order to better understand the molecular basis of inhibitor affinity, we have employed site-directed mutagenesis, enzyme kinetics, and X-ray crystallography to ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801911v

    authors： Shishova EY,Di Costanzo L,Emig FA,Ash DE,Christianson DW

    更新日期：2009-01-13 00:00:00

  • Probing the binding of coumarins and cyclothialidines to DNA gyrase.

    abstract：:DNA gyrase is the target of a number of antibacterial agents, including the coumarins and the cyclothialidines. To extend our understanding of the mechanism of action of these compounds, we have examined the previously published crystal structures of the complexes between the 24 kDa fragment of GyrB and coumarin and c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982320p

    authors： Kampranis SC,Gormley NA,Tranter R,Orphanides G,Maxwell A

    更新日期：1999-02-16 00:00:00

  • The solution structure of Bacillus anthracis dihydrofolate reductase yields insight into the analysis of structure-activity relationships for novel inhibitors.

    abstract：:There is a significant need for new therapeutics to treat infections caused by the biodefense agent Bacillus anthracis. In pursuit of drug discovery against this organism, we have developed novel propargyl-linked inhibitors that target the essential enzyme dihydrofolate reductase (DHFR) from B. anthracis. Previously, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802319w

    authors： Beierlein JM,Deshmukh L,Frey KM,Vinogradova O,Anderson AC

    更新日期：2009-05-19 00:00:00

  • Additive transfer free energies of the peptide backbone unit that are independent of the model compound and the choice of concentration scale.

    abstract：:With knowledge of individual transfer free energies of chemical groups that become newly exposed on protein denaturation and assuming the group transfer free energy contributions are additive, it should be possible to predict the stability of a protein in the presence of denaturant. Unfortunately, several unresolved i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035908r

    authors： Auton M,Bolen DW

    更新日期：2004-02-10 00:00:00

  • Cholesterol Modulates the Formation of the Aβ Ion Channel in Lipid Bilayers.

    abstract：:The misfolding of amyloid beta (Aβ) is one of the predominant hallmarks in the pathology of Alzheimer's disease (AD). In this study, we showed that the formation of the Aβ ion channel on the membrane depended on the cholesterol concentration. From a mechanical aspect, we found that cholesterol levels affected the stab...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00968

    authors： Gao Q,Wu G,Lai KWC

    更新日期：2020-03-03 00:00:00

  • Identification of the sterol- and actin-binding domains of plasma vitamin D binding protein (Gc-globulin).

    abstract：:The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00146a021

    authors： Haddad JG,Hu YZ,Kowalski MA,Laramore C,Ray K,Robzyk P,Cooke NE

    更新日期：1992-08-11 00:00:00

  • The heparin binding domain of S-protein/vitronectin binds to complement components C7, C8, and C9 and perforin from cytolytic T-cells and inhibits their lytic activities.

    abstract：:S-Protein/vitronectin is a serum glycoprotein that inhibits the lytic activity of the membrane attack complex of complement, i.e., of the complex including the proteins C5b, C6, C7, C8, and C9n. We show that intact S-protein/vitronectin or its cyanogen bromide generated fragments also inhibit the hemolysis mediated by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00411a029

    authors： Tschopp J,Masson D,Schäfer S,Peitsch M,Preissner KT

    更新日期：1988-05-31 00:00:00

  • Investigation of the aggregation and activation of prothrombin using quasi-elastic light scattering.

    abstract：:The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00628a016

    authors： Agarwal GP,Gallagher JG,Aune KC,Armeniades CD

    更新日期：1977-05-03 00:00:00

  • Crystal structure of flavocetin-A, a platelet glycoprotein Ib-binding protein, reveals a novel cyclic tetramer of C-type lectin-like heterodimers.

    abstract：:Snake venom contains a number of the hemostatically active C-type lectin-like proteins, which affect the interaction between von Willebrand factor (vWF) and the platelet glycoprotein (GP) Ib or platelet receptor to inhibit/induce platelet activation. Flavocetin-A (FL-A) is a high-molecular mass C-type lectin-like prot...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992134z

    authors： Fukuda K,Mizuno H,Atoda H,Morita T

    更新日期：2000-02-29 00:00:00