Abstract：
:We have developed a model of glycophorin in a phospholipid bilayer membrane in order to study the thermodynamics of this system and to understand the detailed behavior of recent calorimetric data. We assume that the larger glycophorin polar group can be considered as either adopting a pancakelike conformation at the bilayer interface (D state) or be directed generally away from the interface (U state) [Ruppel, D., Kapitza, H.G., Galla, H.J., Sixl, F., & Sackmann, E. (1982) Biochim. Biophys. Acta 692, 1-17]. Lipid hydrocarbon chains are described either as excited (e state) with high energy and relatively many gauche conformers or as generally extended (g state) with low energy. We performed a Monte-Carlo simulation using the Glauber and Kawasaki procedures on a triangular lattice which represents the plane of half of the bilayer. Lattice sites can be occupied either by lipid hydrocarbon chains or by model glycophorin alpha-helical hydrophobic cores. The states D and U are represented by hexagons of different sizes in the plane of the lattice, and the hard core repulsion between two such polar groups is accounted for by forbidding hexagon-hexagon overlap. We have studied the effect of having the glycophorin polar group interact in various ways with the lipid bilayer. We find that the protein polar group in its D state interacts, either directly or indirectly, with the lipid bilayer so as to reduce the effective lateral pressure acting on the lipid hydrocarbon chains by about 3 dyn/cm. Polar groups in their U states do not reduce this lateral pressure.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
MacDonald AL,Pink DAdoi
10.1021/bi00381a019subject
Has Abstractpub_date
1987-04-07 00:00:00pages
1909-17issue
7eissn
0006-2960issn
1520-4995journal_volume
26pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Although insulin and insulin-like growth factor-1 (IGF-1) belong to one family, insulin folds into one thermodynamically stable structure, while IGF-1-folds into two thermodynamically stable structures (native and swap forms). We have demonstrated previously that the bifurcating folding behavior of IGF-1 is mainly con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049710y
更新日期：2004-07-20 00:00:00
abstract：:The turnover of the collagen triple-helical structure (collagenolysis) is a tightly regulated process in normal physiology and has been ascribed to a small number of proteases. Several members of the matrix metalloproteinase (MMPs) family possess collagenolytic activity, and the mechanisms by which these enzymes proce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062199j
更新日期：2007-03-27 00:00:00
abstract：:The expression of rusticyanin in Escherichia coli and a number of mutants for Ser86 is reported. Mutations of Ser86 to Asn, Asp, Gln, and Leu were undertaken as this is an Asn residue in other structurally characterized cupredoxins, and it has been suggested that this may be partly responsible for the high redox poten...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980960m
更新日期：1998-08-18 00:00:00
abstract：:The interaction of linker histone H1 with both linear and superhelical double-stranded DNA has been investigated at low ionic strengths. Gel mobility retardation experiments demonstrate strikingly different behavior for the two forms of DNA. First, the experiments strongly suggest that linker histone binds to superhel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0497704
更新日期：2004-06-22 00:00:00
abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a014
更新日期：1995-02-28 00:00:00
abstract：:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0004596
更新日期：2000-08-08 00:00:00
abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00576a012
更新日期：1979-05-01 00:00:00
abstract：:Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200291e
更新日期：2011-07-12 00:00:00
abstract：:Biochemical properties of a detergent-solubilized tetrodotoxin binding component from Electrophorus electricus have been examined and compared with those found for the membrane-bound protein. The toxin binding component was solubilized with high efficiency by a variety of nonionic detergents and with lower efficiency ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00267a029
更新日期：1982-11-23 00:00:00
abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00176
更新日期：2016-06-14 00:00:00
abstract：:The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9922547
更新日期：2000-02-15 00:00:00
abstract：:Cryptophycin-52 (LY355703) is currently undergoing clinical evaluation for cancer chemotherapy. It is a potent suppressor of microtubule dynamics in vitro, and low picomolar concentrations appear to inhibit cancer cell proliferation at mitosis by stabilizing spindle microtubules. In the present study, using [(3)H]cryp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0010827
更新日期：2000-11-21 00:00:00
abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970513r
更新日期：1997-07-08 00:00:00
abstract：:Photochemical and subsequent thermal reactions of rhodopsin containing 9-cis-retinal [Rh(9)] or one of four analogues with 9-cis geometries formed from ring-modified retinals, alpha-retinal [alpha Rh(9)], acyclic retinal [AcRh(9)], acyclic alpha-retinal [Ac alpha Rh(9)], and 5-isopropyl-alpha-retinal [P alpha Rh(9)] w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00233a022
更新日期：1991-05-14 00:00:00
abstract：:A structural understanding of the nature and scope of serpin inhibition mechanisms has been limited by the inability so far to crystallize any serpin-proteinase complex. We describe here the application of [(1)H-(15)N]-HSQC NMR on uniformly and residue-selectively (15)N-labeled serpin alpha(1)-proteinase inhibitor (Pi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001152+
更新日期：2000-10-03 00:00:00
abstract：:Arginine vasopressin (AVP) is a nonapeptide long known as an endocrine and paracrine regulator of important systemic functions, namely, vasoconstriction, gluconeogenesis, corticosteroidogenesis, and excretion of water and urea. Here we report, for the first time, that AVP specifically inhibits expression of the cyclin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026807g
更新日期：2003-02-25 00:00:00
abstract：:A versatile diatomic physiological messenger, nitric oxide (NO), is biosynthesized by a group of flavo-heme enzymes, the nitric oxide synthases. We have examined the active site of the neuronal isoform by EPR spectroscopy of the ferrous nitric oxide complex. The nitric oxide complex of the substrate-free enzyme exhibi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970823+
更新日期：1997-09-09 00:00:00
abstract：:In iron-deficient conditions, Azomonas macrocytogenes ATCC 12334 excretes a fluorescent siderophore called azoverdin, which is composed of a six-amino-acid peptide chain linked to a chromophore. Azoverdin chelates iron(III) very strongly, solubilizing it and transporting it back into the cells using an outer-membrane ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025990a
更新日期：2002-10-15 00:00:00
abstract：:Ultraviolet resonance Raman (UV RR) spectra are reported for ferricytochrome c from tuna and horse heart at pH 1.6, 7, 10, and 13, representing distinct conformational states of the protein (states II, III, IV, and V, respectively). The spectra were obtained with pulsed laser excitation at 200 and 218 nm, via H2 Raman...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00339a035
更新日期：1985-08-27 00:00:00
abstract：:The major proton-transfer pathway into the buried active site of cytochrome c oxidase (CcO) is the D-pathway that begins with the subunit I residue Asp-132 on the inner protein surface (the cytoplasmic surface of the aa3-type CcO of Rhodobacter sphaeroides). Asp-132 is surrounded by residues from both subunits I and I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0605843
更新日期：2006-07-11 00:00:00
abstract：:Heme propionate-7 in cytochrome c is an ionizable group located in a region of the protein that is inaccessible to bulk solvent. Electrostatic stabilization of this functional group appears to be achieved through interaction of heme propionate-7 with several amino acid residues that occur within hydrogen-bonding dista...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00071a019
更新日期：1993-05-25 00:00:00
abstract：:Mutagenesis of Escherichia coli manganese superoxide dismutase (MnSD) demonstrates involvement of the strictly conserved gateway tyrosine (Y34) in exogenous ligand interactions. Conservative replacement of this residue by phenylalanine (Y34F) affects the pH sensitivity of the active-site metal ion and perturbs ligand ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9704212
更新日期：1997-07-22 00:00:00
abstract：:Reducing sugars such as glucose react nonenzymatically with protein amino groups to initiate a posttranslational modification process known as advanced glycosylation. Nucleotide bases also participate in advanced glycosylation reactions, producing DNA-linked advanced glycosylation endproducts (AGEs) that cause mutatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00002a032
更新日期：1995-01-17 00:00:00
abstract：:In eukaryotic organisms, the largely cytosolic copper- and zinc-containing superoxide dismutase (Cu/Zn SOD) enzyme represents a key defense against reactive oxygen toxicity. Although much is known about the biology of this enzyme under aerobic conditions, less is understood regarding the effects of low oxygen levels o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201353y
更新日期：2012-01-17 00:00:00
abstract：:The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00009a017
更新日期：1995-03-07 00:00:00
abstract：:The filamentous coliphage M13 possesses multiple copies of a 50-residue coat protein which is inserted into the inner membrane of Escherichia coli during infection. 13C nuclear magnetic resonance (NMR) spectroscopy has been used to probe the structure and dynamics of M13 coat protein solubilized in detergent micelles....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00351a012
更新日期：1986-02-11 00:00:00
abstract：:Steady-state and femtosecond time-resolved optical methods have been used to compare the spectroscopic features and energy transfer dynamics of two systematically different light-harvesting complexes from the dinoflagellate Amphidinium carterae: main-form (MFPCP) and high-salt (HSPCP) peridinin-chlorophyll a-proteins....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061217u
更新日期：2006-11-28 00:00:00
abstract：:Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase (Nmt1p) is an essential enzyme that catalyzes the transfer of myristic acid (C14:0) from myristoylCoA to the N-terminus of cellular proteins with a variety of functions. Nmts from an assortment of species display remarkable in vivo specificity for th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970311v
更新日期：1997-06-03 00:00:00
abstract：:Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00123a019
更新日期：1992-03-03 00:00:00
abstract：:The expression level of protein DR1199 is observed to increase considerably in the radio-resistant bacterium Deinococcus radiodurans following irradiation. This protein belongs to the DJ-1 superfamily, which includes proteins with diverse functions, such as the archaeal proteases PhpI and PfpI, the bacterial chaperone...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800882v
更新日期：2008-11-04 00:00:00
