Abstract：
:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cytochrome f was impaired in site-directed mutants of the conserved Asn and Gln residues that form hydrogen bonds with water molecules of the internal chain [Ponamarev, M. V., and Cramer, W. A. (1998) Biochemistry 37, 17199-17208]. The 251-residue extrinsic functional domain of C. reinhardtii cytochrome f was expressed in Escherichia coli without the 35 C-terminal residues of the intact cytochrome that contain the membrane anchor. Crystal structures were determined for the wild type and three "water chain" mutants (N168F, Q158L, and N153Q) having impaired photosynthetic and electron transfer function. The mutant cytochromes were produced, folded, and assembled heme at levels identical to that of the wild type in the E. coli expression system. N168F, which had a non-photosynthetic phenotype and was thus most affected by mutational substitution, also had the greatest structural perturbation with two water molecules (W4 and W5) displaced from the internal chain. Q158L, the photosynthetic mutant with the largest impairment of in vivo electron transfer, had a more weakly bound water at one position (W1). N153Q, a less impaired photosynthetic mutant, had an internal water chain with positions and hydrogen bonds identical to those of the wild type. The structure data imply that the waters of the internal chain, in addition to the surrounding protein, have a significant role in cytochrome f function.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sainz G,Carrell CJ,Ponamarev MV,Soriano GM,Cramer WA,Smith JLdoi
10.1021/bi0004596subject
Has Abstractpub_date
2000-08-08 00:00:00pages
9164-73issue
31eissn
0006-2960issn
1520-4995pii
bi0004596journal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0024759
更新日期：2001-03-06 00:00:00
abstract：:Carbamoyl phosphate synthetase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions occurring at three active sites connected by a molecular tunnel of 100 A. To understand the mechanism for coordination and synchronization among the active sites, the pre-steady-state time cour...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992772h
更新日期：2000-05-02 00:00:00
abstract：:We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00251a030
更新日期：1994-11-29 00:00:00
abstract：:The interaction of factor B with C3b deposited on the surface of pathogens is the first step in the activation of the alternative complement pathway. The role of the von Willebrand factor type A (VWFA) module of factor B in this interaction has been investigated by generating three chimeras, Ch1-Ch3, in which surface ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963155l
更新日期：1997-06-03 00:00:00
abstract：:The outer mitochondrial membrane isoform of mammalian cytochrome b(5) (OM b(5)) is distinguished from the microsomal isoform (Mc b(5)) by its considerably greater stability. In contrast, OM and Mc apocytochrome b(5) (apo-b(5)) exhibit similar thermodynamic stability. Contributing substantially to the greater stability...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051337m
更新日期：2005-11-08 00:00:00
abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00104a022
更新日期：1991-10-08 00:00:00
abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00112a004
更新日期：1991-12-03 00:00:00
abstract：:The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602366n
更新日期：2007-02-27 00:00:00
abstract：:Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901046x
更新日期：2009-09-22 00:00:00
abstract：:The interaction of the recombinant hemoglobin from Vitreoscilla sp. (VHb) with the bacterial membrane of Escherichia coli cells has been investigated by measuring the propensity of VHb to interact with monolayers formed by natural bacterial phosholipids. The measurements showed that the protein is capable of penetrati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052277n
更新日期：2006-04-04 00:00:00
abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00300a002
更新日期：1984-02-28 00:00:00
abstract：:CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9920127
更新日期：1999-12-14 00:00:00
abstract：:The truncated hemoglobins from Bacillus subtilis (Bs-trHb) and Thermobifida fusca (Tf-trHb) have been shown to form high-affinity complexes with hydrogen sulfide in their ferric state. The recombinant proteins, as extracted from Escherichia coli cells after overexpression, are indeed partially saturated with sulfide, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901671d
更新日期：2010-03-16 00:00:00
abstract：:Nucleolin, also called protein C23, is a RNA-associated protein implicated in the early stages of ribosome assembly. To study the general conformation and map the nucleic acid binding regions, rat nucleolin was subjected to limited proteolysis using trypsin and chymotrypsin in the presence or absence of poly(G). The c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00476a006
更新日期：1990-06-19 00:00:00
abstract：:The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00046a023
更新日期：1995-11-21 00:00:00
abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01072
更新日期：2018-12-11 00:00:00
abstract：:Horse heart cytochrome c has been modified by 8-azido-ATP and the electron-transfer activity of the modified cytochrome c's to bovine heart cytochrome c oxidase (CcO) under physiological ionic strengths has been studied by the laser flash photolysis technique with 5-deazariboflavin and EDTA as the electron donor. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a035
更新日期：1995-02-28 00:00:00
abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026778+
更新日期：2003-02-04 00:00:00
abstract：:Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base pairs, and potentially uracils within single-stranded DNA (ssDNA). An interesting qu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301562n
更新日期：2013-04-16 00:00:00
abstract：:Protein C is a vitamin K dependent protein present in bovine plasma (Stenflo, J. (1976), J. Biol. Chem. 251, 355). It is a glycoprotein (mol wt approximately 62 000) composed of a heavy chain (mol wt 41 000) and a light chain (mol wt 21 000). The heavy chain has an amino-terminal sequence of Asp-Thr-Asn-Gln and contai...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00667a022
更新日期：1976-11-02 00:00:00
abstract：:The binding of Ca2+ and the resulting change in catalytic specificity that allows phosphorylation of the calcium ATPase of sarcoplasmic reticulum by ATP were examined by measuring the amount of phosphoenzyme formation from [32P]ATP, or 45Ca incorporation into vesicles, after the simultaneous addition of ATP and EGTA a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00423a018
更新日期：1988-11-15 00:00:00
abstract：:The photochemical and subsequent thermal reactions of the mouse short-wavelength visual pigment (MUV) were studied by using cryogenic UV-visible and FTIR difference spectroscopy. Upon illumination at 75 K, MUV forms a batho intermediate (lambda(max) approximately 380 nm). The batho intermediate thermally decays to the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025883g
更新日期：2002-08-06 00:00:00
abstract：:The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00022a008
更新日期：1995-06-06 00:00:00
abstract：:Human genomic clones that span the entire protein S expressed gene (PS alpha) and the 3' two-thirds of the protein S pseudogene (PS beta) have been isolated and characterized. The PS alpha gene is greater than 80 kilobases in length and contains 14 introns and 15 exons, as well as 6 repetitive "Alu" sequences. Exons I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00486a010
更新日期：1990-08-28 00:00:00
abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00125a022
更新日期：1992-03-17 00:00:00
abstract：:CytA is a member of a functionally defined family of insecticidal delta-endotoxins occurring in parasporal crystals of Bacillus thuringiensis var. israelensis. We investigated the ability of CytA to permeabilize the membrane and release fluorescence marker molecules from unilamellar lipid vesicles. Both protoxin (27 k...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960970s
更新日期：1996-09-03 00:00:00
abstract：:DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500810a
更新日期：2014-08-26 00:00:00
abstract：:Group I intron RNAs contain a core of highly conserved helices flanked by peripheral domains that stabilize the core structure. In the Tetrahymena group I ribozyme, the P4, P5, and P6 helices of the core pack tightly against a three-helix subdomain called P5abc. Chemical footprinting and the crystal structure of the T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982113p
更新日期：1999-03-09 00:00:00
abstract：:A series of monoclonal antibodies specific for distinguishable epitopes in chromosomal protein histone H5 were obtained from mice immunized with either free H5 or H5 . RNA complexes. The antibodies elicited by H5 could be distinguished from those elicited by H5 . RNA by their binding to native or acid-denatured H5, by...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a012
更新日期：1984-07-17 00:00:00
abstract：:Ribosomal protein E-L30 unfolds reversibly at pH values between 7.0 and 4.5. Unfolding of the protein involves a fast and a slow equilibrium, which depend on the degree of protonation of His19 and His33. Both the fast equilibrium between protonated and deprotonated histidines and the slow equilibrium between folded an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00391a050
更新日期：1987-08-25 00:00:00
