Abstract:
:The photochemical and subsequent thermal reactions of the mouse short-wavelength visual pigment (MUV) were studied by using cryogenic UV-visible and FTIR difference spectroscopy. Upon illumination at 75 K, MUV forms a batho intermediate (lambda(max) approximately 380 nm). The batho intermediate thermally decays to the lumi intermediate (lambda(max) approximately 440 nm) via a slightly blue-shifted intermediate not observed in other photobleaching pathways, BL (lambda(max) approximately 375 nm), at temperatures greater than 180 K. The lumi intermediate has a significantly red-shifted absorption maximum at 440 nm, suggesting that the retinylidene Schiff base in this intermediate is protonated. The lumi intermediate decays to an even more red-shifted meta I intermediate (lambda(max) approximately 480 nm) which in turn decays to meta II (lambda(max) approximately 380 nm) at 248 K and above. Differential FTIR analysis of the 1100-1500 cm(-1) region reveals an integral absorptivity that is more than 3 times smaller than observed in rhodopsin and VCOP. These results are consistent with an unprotonated Schiff base chromophore. We conclude that the MUV-visual pigment possesses an unprotonated retinylidene Schiff base in the dark state, and undergoes a protonation event during the photobleaching cascade.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Dukkipati A,Kusnetzow A,Babu KR,Ramos L,Singh D,Knox BE,Birge RRdoi
10.1021/bi025883gsubject
Has Abstractpub_date
2002-08-06 00:00:00pages
9842-51issue
31eissn
0006-2960issn
1520-4995pii
bi025883gjournal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Yeast actin mutants with acidic residues at the N terminus either neutralized (DNEQ) or deleted (delta-DSE) were used to assess the role of N-terminal acidic residues in the interactions of actin with myosin in the contractile cycle. Cosedimentation experiments revealed an approximately 3-fold decrease in the binding ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962388+
更新日期:1996-12-24 00:00:00
abstract::The requirement for Ca2+ in the Mn(2+)-dependent photoactivation of oxygen evolution was re-evaluated using 17 kDa/24 kDa-less photosystem II (PSII) membranes depleted of (Mn)4-clusters by NH2OH extraction. At optimum conditions (1 mM Mn2+/10 microM 2,6-dichlorphenolindophenol (DCIP)/20 mM Ca2+), the light-induced inc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00041a031
更新日期:1995-10-17 00:00:00
abstract::Amyloid beta-protein (Abeta) self-assembly is linked strongly to Alzheimer's disease. We found that PP-Leu, a tridecapeptide analogue of broad-spectrum antiviral peptides termed theta-defensins, potently inhibits Abeta oligomer and fibril formation. This effect appeared to be mediated through sequestration of the amyl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901325g
更新日期:2009-12-08 00:00:00
abstract::The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a016
更新日期:1977-05-03 00:00:00
abstract::Vibrational Raman spectroscopic experiments have been performed as a function of temperature on aqueous dispersions of synthetic DL-erythro-N-lignoceroylsphingosylphosphocholine [C(24):SPM], a racemic mixture of two highly asymmetric hydrocarbon chain length sphingomyelins. Raman spectral peak-height intensity ratios ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00343a036
更新日期:1985-10-22 00:00:00
abstract::The Fpg protein from Escherichia coli is a multifunctional protein that excises damaged purine bases from DNA to generate aldehydic abasic sites and then catalyzes the successive cleavage of the phosphodiester bonds first on the 3'-side and then on the 5'-side of the abasic site to generate 5'- and 3'-phosphate ends, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9522662
更新日期:1996-01-16 00:00:00
abstract::Epitranscriptomic RNA modifications can serve as recognition elements for the recruitment of effector proteins (i.e., "readers") to modified transcripts. While these interactions play an important role in mRNA regulation, there is a major gap in our understanding of the sequence determinants critical for the binding o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00485
更新日期:2019-08-06 00:00:00
abstract::We report that recombinant glia maturation factor (GMF), a 17-kDa brain protein, inhibits the activity of mitogen-activated protein (MAP) kinase in the test tube assay, in particular the ERK1/ERK2 isoforms. A preliminary phosphorylation of GMF by protein kinase A (PKA) dramatically increases its inhibitory effect by o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960034c
更新日期:1996-05-21 00:00:00
abstract::Tubulin polymerization promoting proteins (TPPPs) constitute a eukaryotic protein family. There are three TPPP paralogs in the human genome, denoted as TPPP1-TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules, but TPPP2 does not. Vertebrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00902
更新日期:2017-02-21 00:00:00
abstract::The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled nativelike functional arrays of an aspartate receptor cytoplasmic fragment (CF) with its two cytoplasmic protein partners (CheA and Ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00234
更新日期:2016-07-05 00:00:00
abstract::The reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) is accompanied by exceptional values for rate enhancement (k(cat)/k(non) = 7.1 × 10(16)) and catalytic proficiency [(k(cat)/K(M))/k(non) = 4.8 × 10(22) M(-1)]. Although a stabilized vinyl carbanion/carbene intermediate is located on the reactio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2012355
更新日期:2011-10-04 00:00:00
abstract::A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00649a016
更新日期:1976-02-24 00:00:00
abstract::To be fully active and participate in the metabolism of phosphorylated nucleotides, most nucleoside diphosphate kinases (NDPKs) have to assemble into stable hexamers. Here we studied the role played by six intersubunit salt bridges R80-D93 in the stability of NDPK from the pathogen Mycobacterium tuberculosis ( Mt). Mu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01308
更新日期:2019-03-12 00:00:00
abstract::Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00431
更新日期:2015-07-07 00:00:00
abstract::Regulation of adenylate cyclase by alpha 2 adrenergic receptors requires the inhibitory guanine nucleotide binding protein Ni. A role for this protein has also been suggested in the high-affinity binding of agonists to the alpha 2 receptor. We recently reported that alkaline treatment can selectively inactivate alpha ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00386a061
更新日期:1987-06-16 00:00:00
abstract::(S)-Mandelate dehydrogenase from Pseudomonas putida belongs to a FMN-dependent enzyme family that oxidizes (S)-alpha-hydroxyacids. Despite a high degree of sequence and structural similarity, this family can be divided into three subgroups based on the different oxidants utilized in the second oxidative half-reaction....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049005p
更新日期:2004-08-24 00:00:00
abstract::We describe a method for isolating milligram quantities of assembly-competent tubulin from the budding yeast Saccharomyces cerevisiae. The tubulin is > 95% purified and free of contaminating enzyme activities. As a result, it has been possible to determine the yeast tubulin equilibrium-binding constant for Mg-GTP and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00085a013
更新日期:1993-08-31 00:00:00
abstract::We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963124q
更新日期:1997-06-17 00:00:00
abstract::Cells can be transiently permeabilized by application of high electric pulses of short duration. A direct consequence of this treatment is to induce a fusogenic state in the pulsed membrane. The molecular events underlying these phenomena remain to be explained. During our work, we investigated the effects of pulsing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00471a009
更新日期:1990-05-15 00:00:00
abstract::NADPH-cytochrome P450 oxidoreductase (P450 reductase, EC 1.6.2.4) is an essential component of the P450 monooxygenase complex and binds FMN, FAD, and NADPH cofactors. Residues Tyr140 and Tyr178 are known to be involved in FMN binding. A third aromatic side chain, Phe181, is also located in the proximity of the FMN rin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011147l
更新日期:2001-11-13 00:00:00
abstract::The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00163a007
更新日期:1992-12-08 00:00:00
abstract::The X-ray crystal structure of recombinant Desulfovibrio vulgaris rubrerythrin (Rbr) that was subjected to metal constitution first with zinc and then iron, yielding ZnS(4)Rbr, is reported. A [Zn(SCys)(4)] site with no iron and a diiron site with no appreciable zinc in ZnS(4)Rbr were confirmed by analysis of the anoma...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0356193
更新日期:2004-03-23 00:00:00
abstract::Histamine is released from mast cells and basophils by either immunological or nonimmunological mechanisms. Histamine, which is the most potent short acting mediator released from these cells, exerts its diverse biological actions by binding to cell surface histamine receptors. We report the affinity purification of h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00182a008
更新日期:1994-04-26 00:00:00
abstract::Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0347104
更新日期:2004-02-17 00:00:00
abstract::Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011322y
更新日期:2001-11-06 00:00:00
abstract::Herpesvirus saimiri encodes a tyrosine kinase interacting protein (Tip) that binds to T-cell-specific tyrosine kinase Lck via multiple sequence motifs and controls its activity. The regulation of Lck by Tip represents a key mechanism in the transformation of human T-lymphocytes during herpesviral infection. In this st...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485068
更新日期:2004-11-30 00:00:00
abstract::The intercellular environment is known to be very different from the environment where most of the elementary biological processes are studied in the laboratory. As a result, there was a considerable effort on cell mimicking either by confinement or by introducing macromolecular crowding. In the present study, dextran...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00599
更新日期:2018-10-23 00:00:00
abstract::A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3016839
更新日期:2013-04-09 00:00:00
abstract::To shed more light on the molecular requirements for recognition of thyroid response elements (TREs) by thyroid receptors (TRs), we compared the specific aspects of DNA TRE recognition by different TR constructs. Using fluorescence anisotropy, we performed a detailed and hierarchical study of TR-TRE binding. This was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901282s
更新日期:2010-02-09 00:00:00
abstract::Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00521
更新日期:2018-07-31 00:00:00