Abstract:
:Histamine is released from mast cells and basophils by either immunological or nonimmunological mechanisms. Histamine, which is the most potent short acting mediator released from these cells, exerts its diverse biological actions by binding to cell surface histamine receptors. We report the affinity purification of histamine receptor proteins from Triton X-100 solubilized peripheral human blood mononuclear cells which include lymphocytes and monocytes. Three different designs of histamine affinity columns were constructed; all three resulted in the same material being eluted. This consisted of bands which on SDS-PAGE after boiling and reduction had the following molecular weights: 193K, 84K, 58K, 48K, 37K, and 16K. The most abundant bands were of molecular weights 193K, 48K, and 16K, and these were disulfide bonded together to form a high molecular weight complex. (The 58K band was present in lower amounts than the others, and in only a few fractions. It had the same molecular weight as the dimeric form of histamine methyltransferase which is present in small amounts in mononuclear cells and may therefore have copurified.) The histamine binding proteins described in this report were purified by conventional affinity chromatography, rather than by an expression cloning approach which obviates the use of any protein chemistry. Consequently, we had the advantage of being able to verify the histamine binding specificity of our purified proteins directly and with several independent assays as follows. The histamine binding specificity of all three columns was established by specific elution with histamine, by preabsorption of crude cell extract with excess free histamine prior to column application, and by comparison with control columns. Independent determination of the binding specificity, using a radioreceptor dot blot assay, of the eluate containing only the 193K, 48K, and 16K disulfide-linked subunits confirmed that the purified material bound specifically to [3H]histamine and that a 300-500-fold degree of purification from tissue extract had been obtained. Following cell surface radioreceptor cross-linking of radiolabeled histamine to intact mononuclear cells, the 16K band was detected, indicating it to be the ligand-binding subunit for histamine. These same three proteins were purified from T lymphocyte and monocytoid cell lines, indicating that both lymphocyte and monocyte subsets of mononuclear cells express these proteins.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Warlow RS,Rajasekariah P,Lambert P,Morgan J,Dao LP,Bernard CC,Walls RSdoi
10.1021/bi00182a008subject
Has Abstractpub_date
1994-04-26 00:00:00pages
4800-11issue
16eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Triple-helical DNA shows increasing potential for applications in the control of gene expression (including therapeutics) and the development of sequence-specific DNA-cleaving agents. The major limitation in this technology has been the requirement of homopurine sequences for triplex formation. We describe a simple ap...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00117a002
更新日期:1992-01-21 00:00:00
abstract::The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The env...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00662a033
更新日期:1976-08-24 00:00:00
abstract::Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702250h
更新日期:2008-03-18 00:00:00
abstract::The enzyme form of the calcium adenosinetriphosphatase of sarcoplasmic reticulum (CaATPase) that is stable in the presence of calcium, cE.Ca2, has a binding site for the catalytic Mg2+ ion with a dissociation constant of 0.94 +/- 0.15 mM at 25 degrees C, pH 7.0, and 100 mM KCl. This is approximately 10 times smaller t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00077a016
更新日期:1993-07-06 00:00:00
abstract::We have shown previously that the hepatic and intestinal transcription of the human apolipoprotein A-II (apoA-II) gene in cell cultures is controlled by a complex set of regulatory elements A-N [Chambaz, et al. (1991) J. Biol. Chem. 266, 11676-11685; Cardot, et al. (1991) J. Biol. Chem. 266, 24460-24470]. In the prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00086a013
更新日期:1993-09-07 00:00:00
abstract::A detailed characterization of the main chain and side chain dynamics in R. capsulatus ferrocytochrome c(2) derived from (2)H NMR relaxation of methyl group resonances is presented. (15)N relaxation measurements confirm earlier results indicating that R. capsulatus ferrocytochrome c(2) exhibits minor rotational anisot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0102252
更新日期:2001-06-05 00:00:00
abstract::NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3007059
更新日期:2012-09-18 00:00:00
abstract::Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0519194
更新日期:2006-01-17 00:00:00
abstract::Tropomyosin is postulated to confer stability to actin filaments in nonmuscle cells. We have found that a nonmuscle tropomyosin isolated from the intestinal epithelium can directly stabilize actin filaments by slowing depolymerization from the pointed, or slow-growing, filament end. Kinetics of elongation and depolyme...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a035
更新日期:1989-10-17 00:00:00
abstract::Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. It displays no significant sequence homology to other known exchange factors for small G-proteins. Studies are reported here of the mechanism ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0007573
更新日期:2000-09-19 00:00:00
abstract::The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00652a031
更新日期:1976-04-06 00:00:00
abstract::The self-assembly of beta(2)-microglobulin into fibrils leads to dialysis-related amyloidosis. pH-mediated partial unfolding is required for the formation of the amyloidogenic intermediate that then self-assembles into amyloid fibrils. Two partially folded intermediates of beta(2)-microglobulin have been identified ex...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050731h
更新日期:2005-12-13 00:00:00
abstract::We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00297a005
更新日期:1984-01-17 00:00:00
abstract::The thermodynamic quantities of the anti-dinitrophenyl antibody-hapten interaction are reported for rabbit, goat, and guinea pig antibodies. Rabbit and goat antibodies had similar exothermic enthalpy changes for their reaction with 2,4-dinitrophenyl-L-lysine (-13.9 and -14.8 kcal/mol, respectively). The enthalpy chang...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00694a032
更新日期:1975-11-18 00:00:00
abstract::Segregation of mRNAs in the cytoplasm of polar cells has been demonstrated for proteins involved in Xenopus and Drosophila oogenesis, and for some proteins in somatic cells. It is assumed that vectorial transport of the messages is generally responsible for this localization. The mRNA encoding the basic protein of cen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9800247
更新日期:1998-05-12 00:00:00
abstract::Bleeding, the most serious complication of thrombolytic therapy with tissue-type plasminogen activator (t-PA), is thought to result from lysis of fibrin in hemostatic plugs and from the systemic lytic state caused by unopposed plasmin. One mechanism by which systemic plasmin can impair hemostasis is by partially degra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0525730
更新日期:2006-04-04 00:00:00
abstract::Phenylalanine hydroxylase (PheH) and tryptophan hydroxylase (TrpH) catalyze the aromatic hydroxylation of phenylalanine and tryptophan, forming tyrosine and 5-hydroxytryptophan, respectively. The reactions of PheH and TrpH have been investigated with [4-(2)H]-, [3,5-(2)H(2)]-, and (2)H(5)-phenylalanine as substrates. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0607554
更新日期:2006-09-12 00:00:00
abstract::Glu58 is known to participate in phosphodiester transesterification catalyzed by the enzyme RNase T1. For Glu58 RNase T1, an altered mechanism has been proposed in which His40 replaces Glu58 as the base catalyst [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-9072]. Glu58Ala Rnase T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00173a006
更新日期:1994-02-22 00:00:00
abstract::The interactions of Hoechst 33377 (H1) with 20 different oligomeric duplexes have been investigated via spectrofluorometric titrations and/or thermal denaturation experiments. H1 is shown to form 2:1 complexes with dsDNA binding sites of at least four contiguous A/T base pairs. H1 is also shown to possess the rare abi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0103415
更新日期:2001-05-29 00:00:00
abstract::The erythropoietin (EPO) molecule contains four carbohydrate chains. Three contain N-linkages to asparagines at positions 24, 38, and 83, and one contains an O-linkage to a serine at position 126. We constructed human EPO variants that eliminated the three N-glycosylation sites by replacing the asparagines with glutam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00156a003
更新日期:1992-10-20 00:00:00
abstract::Metabolism of [2-13C]-, [3-13C]-, and [1,2,3-13C]propionate in perfused rat livers and [2-13C]-acetate in perfused rat hearts has been examined in tissue extracts by 13C NMR. Label from [2-13C]-propionate was preferentially incorporated into the C2 carbon of lactate, alanine, and aspartate in liver tissue while label ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00186a029
更新日期:1994-05-24 00:00:00
abstract::L-Enduracididine (L-End) is a nonproteinogenic amino acid found in a number of bioactive peptides, including the antibiotics teixobactin, enduracidin, and mannopeptimycin. The potent activity of these compounds against antibiotic-resistant pathogens like MRSA and their novel mode of action have garnered considerable i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01016
更新日期:2015-12-01 00:00:00
abstract::The binding of Zn2+ to tubulin and the ability of this cation to promote the polymorphic assembly of the protein were examined. Equilibrium binding showed the existence of more than 60 potential Zn2+ binding sites on the dimer, including a number of high-affinity sites. The number of high-affinity sites, estimated by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00270a032
更新日期:1983-01-04 00:00:00
abstract::The Fo membrane domain of the F1Fo-ATP synthase complex has been purified from bovine heart mitochondria. The purification procedure involves the removal of peripheral membrane proteins, including F1-ATPase, from submitochondrial particles with guanidine hydrochloride, followed by extraction of Fo and other membrane p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00191a026
更新日期:1994-06-28 00:00:00
abstract::Limited digestion of polysomal RNA with pancreatic ribonuclease releases a structure consisting of poly(A) associated with other polyribonucleotide sequences. This complex can be purified by oligo(dT)-cellulose chromatography. Heating for formamide treatment causes the dissociation of fragments free of poly(A) from th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00686a024
更新日期:1975-07-29 00:00:00
abstract::Biochemical experiments, using the well-defined human liver CYP2C9 expressed in yeast, and molecular modeling techniques were used to derive a predictive model for substrates of CYP2C9. The ability of 10 2-aroylthiophenes related to tienilic acid to act as substrates for CYP2C9 was studied. Four of them were original ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00033a007
更新日期:1995-08-22 00:00:00
abstract::To gain insight into the physiological function of phosphoinositide 3-kinase (PI 3-kinase) lipid products, this study examines the interactions of the D-3 phosphoinositides with profilin and the consequent effects on actin dynamics and phosphoinositide turnover. Profilin, a ubiquitous actin-regulating protein, plays a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961878z
更新日期:1996-11-05 00:00:00
abstract::Homoserine transacetylase catalyzes one of the required steps in the biosynthesis of methionine in fungi and several bacteria. We have determined the crystal structure of homoserine transacetylase from Haemophilus influenzae to a resolution of 1.65 A. The structure identifies this enzyme to be a member of the alpha/be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051951y
更新日期:2005-12-06 00:00:00
abstract::Natriuretic peptide receptor A (NPRA) is a noncovalent homodimeric receptor, composed of an extracellular domain (ECD) with a ligand-binding site, a single transmembrane domain (TM), and an intracellular domain (ICD) exhibiting guanylyl cyclase activity. NPRA activation by atrial natriuretic peptide (ANP) leads to cGM...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901711w
更新日期:2010-06-08 00:00:00
abstract::Protein farnesyltransferase catalyzes the posttranslational farnesylation of several proteins involved in signal transduction, including Ras, and is a target enzyme for antitumor therapies. Efficient product formation catalyzed by protein farnesyltransferase requires an enzyme-bound zinc cation and high concentrations...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0011781
更新日期:2000-10-10 00:00:00