Crystal structure of homoserine transacetylase from Haemophilus influenzae reveals a new family of alpha/beta-hydrolases.

abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...

journal_title：Biochemistry

authors： Schurter EJ,Sayer JM,Oh-hara T,Yeh HJ,Yagi H,Luxon BA,Jerina DM,Gorenstein DG

更新日期：1995-07-18 00:00:00

abstract：:The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...

journal_title：Biochemistry

authors： Zimmermann JL,Amano T,Sigalat C

更新日期：1999-11-16 00:00:00

abstract：:Mutagenesis of Escherichia coli manganese superoxide dismutase (MnSD) demonstrates involvement of the strictly conserved gateway tyrosine (Y34) in exogenous ligand interactions. Conservative replacement of this residue by phenylalanine (Y34F) affects the pH sensitivity of the active-site metal ion and perturbs ligand ...

journal_title：Biochemistry

authors： Whittaker MM,Whittaker JW

更新日期：1997-07-22 00:00:00

abstract：:Platelet receptor recognition domains are located on the gamma and alpha chains of human fibrinogen. The former encompasses residues 400-411 [Kloczewiak, M., Timmons, S., Lukas, T. J., & Hawiger, J. (1984) Biochemistry 23, 1767], and the latter is present in two loci on the alpha chain (alpha 95-97 and alpha 572-574) ...

journal_title：Biochemistry

authors： Timmons S,Bednarek MA,Kloczewiak M,Hawiger J

更新日期：1989-04-04 00:00:00

abstract：:SH2 domain-containing inositol 5-phosphatases 1 (SHIP1) and 2 (SHIP2) are structurally similar proteins that catalyze the degradation of lipid secondary messenger phosphatidylinositol 3,4,5-triphosphate to produce phosphatidylinositol 3,4-diphosphate. Despite their high sequence identity (51%), SHIP1 and SHIP2 share l...

journal_title：Biochemistry

authors： Zhang Y,Wavreille AS,Kunys AR,Pei D

更新日期：2009-11-24 00:00:00

abstract：:Amyloid beta (Abeta) peptide amyloidogenesis, involving the formation of numerous distinct quaternary structures, appears to cause Alzheimer's disease. However, the precise identification of the toxic structure(s) and the neurotoxicity mechanism(s) remains elusive. Mutating the Abeta 1-40 Phe19-Phe20 backbone amide bo...

journal_title：Biochemistry

authors： Bieschke J,Siegel SJ,Fu Y,Kelly JW

更新日期：2008-01-08 00:00:00

abstract：:In order to quantitate the contributions of the polar, uncharged amino acids to the stability of the native state of staphylococcal nuclease, each of the 13 alanines, 9 glycines, 9 threonines, 6 prolines, 6 glutamines, 6 asparagines, and 3 serines was substituted, either with both alanine and glycine or with 1 of thes...

journal_title：Biochemistry

authors： Green SM,Meeker AK,Shortle D

更新日期：1992-06-30 00:00:00

abstract：:The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...

journal_title：Biochemistry

authors： Tse TP,Morris HP,Taylor JM

更新日期：1978-07-25 00:00:00

abstract：:The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...

journal_title：Biochemistry

authors： Benson TE,Marquardt JL,Marquardt AC,Etzkorn FA,Walsh CT

更新日期：1993-03-02 00:00:00

abstract：:Cathepsin K is a cysteine protease present in human osteoclasts that plays an important role in bone resorption. Cathepsin K is synthesized as an inactive proenzyme and activated under conditions of low pH. Autoproteolytic processing of the N-terminal 99 amino acid propeptide produces the active, mature form of cathep...

journal_title：Biochemistry

authors： LaLonde JM,Zhao B,Janson CA,D'Alessio KJ,McQueney MS,Orsini MJ,Debouck CM,Smith WW

更新日期：1999-01-19 00:00:00

abstract：:Comparative mutagenesis studies of N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) adducts positioned in the Nar I restriction enzyme site were performed using Escherichia coli (E. coli) and simian kidney (COS-7) cells. Oligodeoxynucleotides ((5)(')TCCTCG(...

journal_title：Biochemistry

authors： Tan X,Suzuki N,Grollman AP,Shibutani S

更新日期：2002-12-03 00:00:00

abstract：:We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) correlating with major drug resistance, M46L, G48V, and V82S, and five o...

journal_title：Biochemistry

authors： Park JH,Sayer JM,Aniana A,Yu X,Weber IT,Harrison RW,Louis JM

更新日期：2016-04-26 00:00:00

abstract：:Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly Papilio xuthus. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog Xenopus laevis. We designed an 18-residue hybrid pep...

journal_title：Biochemistry

authors： Shin A,Lee E,Jeon D,Park YG,Bang JK,Park YS,Shin SY,Kim Y

更新日期：2015-06-30 00:00:00

abstract：:Marijuana is the most widely abused illegal drug, and its spectrum of effects suggests that several receptors are responsible for the activity. Two cannabinoid receptor subtypes, CB1 and CB2, have been identified, but the complex pharmacological properties of exogenous cannabinoids and endocannabinoids are not fully e...

journal_title：Biochemistry

authors： Kotsikorou E,Madrigal KE,Hurst DP,Sharir H,Lynch DL,Heynen-Genel S,Milan LB,Chung TD,Seltzman HH,Bai Y,Caron MG,Barak L,Abood ME,Reggio PH

更新日期：2011-06-28 00:00:00

abstract：:Flavocytochrome b2 from Saccharomyces cerevisiae couples L-lactate dehydrogenation to cytochrome c reduction. At 25 degrees C, 0.10 M ionic strength, and saturating L-lactate concentration, the turnover rate is 207 s-1 [per cytochrome c reduced; Miles, C. S., Rouviere, N., Lederer, F., Mathews, F. S., Reid, G. A., Bla...

journal_title：Biochemistry

authors： Daff S,Sharp RE,Short DM,Bell C,White P,Manson FD,Reid GA,Chapman SK

更新日期：1996-05-21 00:00:00

abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...

journal_title：Biochemistry

authors： Johnson ME

更新日期：1978-04-04 00:00:00

abstract：:A stable green heme was extracted from ferric cyanosulfmyoglobin after it had undergone an internal conversion reaction. After iron removal and conversion to the methyl ester, the resulting green porphyrin was purified by high-pressure liquid chromatography. Visible, 1H NMR, and mass spectrometric studies provided evi...

journal_title：Biochemistry

authors： Bondoc LL,Chau MH,Price MA,Timkovich R

更新日期：1986-12-30 00:00:00

abstract：:Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...

journal_title：Biochemistry

authors： Oldfield E,Meadows M,Rice D,Jacobs R

更新日期：1978-07-11 00:00:00

abstract：:During T cell signaling, Itk selectively phosphorylates a tyrosine within its own SH3 domain and a tyrosine within PLCgamma1. We find that the remote SH2 domain in each of these substrates is required to achieve efficient tyrosine phosphorylation by Itk and extend this observation to two other Tec family kinases, Btk ...

journal_title：Biochemistry

authors： Joseph RE,Min L,Xu R,Musselman ED,Andreotti AH

更新日期：2007-05-08 00:00:00

abstract：:FixL is a heme-based oxygen-sensing histidine kinase that induces the expression of nitrogen fixation genes under hypoxic conditions. Oxygen dissociation from heme iron in the sensor domain of FixL initiates protein conformational changes that are transmitted to the histidine kinase domain, activating autophosphorylat...

journal_title：Biochemistry

authors： Yamawaki T,Ishikawa H,Mizuno M,Nakamura H,Shiro Y,Mizutani Y

更新日期：2016-07-26 00:00:00

abstract：:Thermobifida fusca Cel9A-90, an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely linked to a family 3c cellulose binding domain (Cel9A-68) followed by a fibronectin III-like domain and a family 2 cellulose binding domain. To study its catalytic mechanism, 12 mutant genes wit...

journal_title：Biochemistry

authors： Zhou W,Irwin DC,Escovar-Kousen J,Wilson DB

更新日期：2004-08-03 00:00:00

abstract：:Novel sulfated tetrasaccharide structures containing 3-O-sulfated GlcA were isolated recently from king crab cartilage chondroitin sulfate K [Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., & Dell, A. (1996) J. Biol. Chem. 271, 26745-26754]. In this study, we prepared a seri...

journal_title：Biochemistry

authors： Kitagawa H,Tanaka Y,Yamada S,Seno N,Haslam SM,Morris HR,Dell A,Sugahara K

更新日期：1997-04-01 00:00:00

abstract：:Cumene hydroperoxide-mediated (CuOOH-mediated) inactivation of cytochromes P450 (CYPs) results in destruction of their prosthetic heme to reactive fragments that irreversibly bind to the protein. We have attempted to characterize this process structurally, using purified, 14C-heme labeled, recombinant human liver P450...

journal_title：Biochemistry

authors： He K,Bornheim LM,Falick AM,Maltby D,Yin H,Correia MA

更新日期：1998-12-15 00:00:00

abstract：:In an effort to better understand protein-protein photoaffinity cross-linking using aryl azides, we have tested a number of factors influencing the cross-linking of the sigma 70 subunit of Escherichia coli RNA polymerase to core RNA polymerase. These factors include the effect of the incubations necessary for the deri...

journal_title：Biochemistry

authors： McMahan SA,Burgess RR

更新日期：1994-10-11 00:00:00

abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus with a rapidly expanding global distribution. Infection can cause severe neurological disease and fatality in humans. Efforts are ongoing to develop antiviral drugs that inhibit the WNV protease, a viral enzyme required for polyprotein processing. Unfortunately, lit...

journal_title：Biochemistry

authors： Garces AP,Watowich SJ

更新日期：2013-10-01 00:00:00

abstract：:The search for a rational method of developing an antimalarial vaccine (malaria caused by Plasmodium falciparum) consists of blocking receptor-ligand interaction. Conserved peptides derived from proteins involved in invasion and having strong red blood cell binding ability have thus been identified; immunization studi...

journal_title：Biochemistry

authors： Patarroyo ME,Alba MP,Vargas LE,Silva Y,Rosas J,Rodríguez R

更新日期：2005-05-10 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...

journal_title：Biochemistry

authors： Keller D,Clausen R,Josefsen K,Led JJ

更新日期：2001-09-04 00:00:00

abstract：:It is now well established that guanine nucleotides are allosteric effectors of the vinca alkaloid-induced self-association of tubulin. GDP enhances self-association for vinblastine-, vincristine- and vinorelbine-induced spiral assembly relative to GTP by 0.90 +/- 0.17 kcal/mol [Lobert et al. (1996) Biochemistry 35, 6...

journal_title：Biochemistry

authors： Vulevic B,Lobert S,Correia JJ

更新日期：1997-10-21 00:00:00

abstract：:The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...

journal_title：Biochemistry

authors： Michelangeli F,Orlowski S,Champeil P,East JM,Lee AG

更新日期：1990-03-27 00:00:00