Structure of a stable form of sulfheme.

abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

journal_title：Biochemistry

authors： Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

更新日期：1997-11-18 00:00:00

abstract：:The proteins which have been left tightly bound to the tissue culture substrate after ethylenebis (oxyethyl-enenitrilo) tetraacetic acid (EGTA)-mediated removal of normal, virus-transformed, and revertant mouse cells and which have been implicated in the substrate adhesion process have been analyzed by slab sodium dod...

journal_title：Biochemistry

authors： Culp LA

更新日期：1976-09-07 00:00:00

abstract：:The selenoamino acids methylselenocysteine (MeSeCys) and selenomethionine (SeMet) have disparate efficacies as anticancer agents. Herein, we use X-ray absorption spectroscopy to determine the chemical form of selenium in human neuroblastoma cells. Cells treated with MeSeCys contain a significant diselenide component, ...

journal_title：Biochemistry

authors： Weekley CM,Aitken JB,Musgrave IF,Harris HH

更新日期：2012-01-24 00:00:00

abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

journal_title：Biochemistry

authors： Skancke J,Bar N,Kuiper M,Hsu LM

更新日期：2015-07-21 00:00:00

abstract：:A new strategy to form stable and sequence-specific triple helical DNA structures at mixed purine/pyrimidine sequences using a combination of four C-glycosides (TRIPsides) has been described [Li et al. (2003) J. Am. Chem. Soc. 125, 2084]. The partial realization of the approach is demonstrated by incorporating two of ...

journal_title：Biochemistry

authors： Li JS,Shikiya R,Marky LA,Gold B

更新日期：2004-02-17 00:00:00

abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...

journal_title：Biochemistry

authors： Bailey DB,Ellis PD,Fee JA

更新日期：1980-02-05 00:00:00

abstract：:c-Myc is a member of a family of sequence specific-DNA binding proteins that are thought to regulate the transcription of genes involved in normal cell growth, differentiation, and apoptosis. In order to understand how human c-myc functions as a transcription factor, we have studied the mechanism of action and structu...

journal_title：Biochemistry

authors： McEwan IJ,Dahlman-Wright K,Ford J,Wright AP

更新日期：1996-07-23 00:00:00

abstract：:Folylpoly-gamma-glutamate synthetase (FPGS) is the enzyme responsible for metabolic trapping of reduced folate cofactors in cells for use in nucleotide and amino acid biosynthesis. There are two isoforms of FPGS expressed in mouse tissues, one is expressed in differentiated tissue, principally liver and kidney, and th...

journal_title：Biochemistry

authors： Andreassi JL 2nd,Moran RG

更新日期：2002-01-08 00:00:00

abstract：:Bleeding, the most serious complication of thrombolytic therapy with tissue-type plasminogen activator (t-PA), is thought to result from lysis of fibrin in hemostatic plugs and from the systemic lytic state caused by unopposed plasmin. One mechanism by which systemic plasmin can impair hemostasis is by partially degra...

journal_title：Biochemistry

authors： Schaefer AV,Leslie BA,Rischke JA,Stafford AR,Fredenburgh JC,Weitz JI

更新日期：2006-04-04 00:00:00

abstract：:The hydrogen exchange kinetics of the complex of trypsin-soybean trypsin inhibitor (Kunitz) have been compared to the calculated sum of the exchange kinetics for the inhibitor and trypsin measured separately. The exchange rates observed for the complex are substantially less than the sum of the exchange rates in the t...

journal_title：Biochemistry

authors： Woodward CK,Ellis LM

更新日期：1975-07-29 00:00:00

abstract：:Photosynthetic water oxidation takes place at the Mn4CaO5 cluster in photosystem II. Around the Mn4CaO5 cluster, a hydrogen bond network is formed by several water molecules, including four water ligands. To clarify the role of this water network in the mechanism of water oxidation, we investigated the effects of the ...

journal_title：Biochemistry

authors： Nakamura S,Ota K,Shibuya Y,Noguchi T

更新日期：2016-01-26 00:00:00

abstract：:Dynamin is a large GTPase involved in the regulation of membrane constriction and fission during receptor-mediated endocytosis. Dynamin contains a pleckstrin-homology domain which is essential for endocytosis and which binds to anionic phospholipids. Here, we show for the first time that dynamin is a membrane-active m...

journal_title：Biochemistry

authors： Burger KN,Demel RA,Schmid SL,de Kruijff B

更新日期：2000-10-10 00:00:00

abstract：:Molecular dynamics simulations employing a molecular mechanics (MM) force field and hybrid quantum mechanics (QM) and MM (QM/MM) have been carried out to investigate the product specificity and mechanism of the histone H4 lysine 20 (H4-K20) methylation by human histone lysine methyltransferase SET8. At neutral pH, the...

journal_title：Biochemistry

authors： Zhang X,Bruice TC

更新日期：2008-06-24 00:00:00

abstract：:Pulmonary surfactant forms a monolayer of lipids and proteins at the alveolar air/liquid interface. Although cholesterol is a natural component of surfactant, its function in surface dynamics is unclear. To further elucidate the role of cholesterol in surfactant, we used a captive bubble surfactometer (CBS) to measure...

journal_title：Biochemistry

authors： Diemel RV,Snel MM,Van Golde LM,Putz G,Haagsman HP,Batenburg JJ

更新日期：2002-12-17 00:00:00

abstract：:FTIR-difference spectroscopy in combination with site-directed mutagenesis has been used to investigate the role of water during the photocycle of bacteriorhodopsin. At least one water molecule is detected which undergoes an increase in H-bonding during the primary bR-->K phototransition. Bands due to water appear in ...

journal_title：Biochemistry

authors： Fischer WB,Sonar S,Marti T,Khorana HG,Rothschild KJ

更新日期：1994-11-01 00:00:00

abstract：:The interaction of bee venom melittin with dimyristolphosphatidylcholine (DMPC) selectively deuteriated in the choline head group has been studied by deuterium and phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. The action of residual phospholipase A2 in melittin samples resulted in mixtures of DMPC and i...

journal_title：Biochemistry

authors： Dempsey CE,Watts A

更新日期：1987-09-08 00:00:00

abstract：:myo-Inositol (mI) is widely distributed in all domains of life and is important for several cellular functions, including bacterial survival. The enzymes responsible for the bacterial catabolism of mI, encoded in the iol operon, can vary from one organism to another, and these pathways have yet to be fully characteriz...

journal_title：Biochemistry

authors： Ramos-Figueroa JS,Aamudalapalli HB,Jagdhane RC,Smith J,Palmer DRJ

更新日期：2020-08-18 00:00:00

abstract：:Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participated in the early phase of blood coagulation. From two genomic libraries, we succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and r...

journal_title：Biochemistry

authors： Beaubien G,Rosinski-Chupin I,Mattei MG,Mbikay M,Chrétien M,Seidah NG

更新日期：1991-02-12 00:00:00

abstract：:Simple procedures for the anaerobic preparation of photoactive and stable P840 reaction centers from Chlorobium tepidum and Chlorobium limicola in good yield are presented and quantitated. The subunit composition was tested by cosedimentation in sucrose density gradients. For C. limicola, it minimally comprises four s...

journal_title：Biochemistry

authors： Hager-Braun C,Xie DL,Jarosch U,Herold E,Büttner M,Zimmermann R,Deutzmann R,Hauska G,Nelson N

更新日期：1995-07-25 00:00:00

abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...

journal_title：Biochemistry

authors： Chang CY,Stellwagen NC

更新日期：2011-10-25 00:00:00

abstract：:Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...

journal_title：Biochemistry

authors： Lijk LJ,Kalk KH,Brandenburg NP,Hol WG

更新日期：1983-06-07 00:00:00

abstract：:The Mn4 complex which is involved in water oxidation in photosystem II (PSII) is known to exhibit two types of EPR signals in the S2 state, one of the five redox states of the enzyme cycle: either a multiline signal (S = 1/2) or a signal at g = 4.1 (S = 3/2 or S= 5/2). The S = 1/2 state can be converted to that respon...

journal_title：Biochemistry

authors： Boussac A,Un S,Horner O,Rutherford AW

更新日期：1998-03-24 00:00:00

abstract：:We have found a new class of inhibitors of the bacterial bioluminescence reaction, the N,N-diphenylalkylamines and acids. We have studied the action of one of these compounds 2,2-diphenylpropylamine. The amine was competitive with the long-chain aliphatic aldehyde substrate (Ki congruent to 0.1 mM) but caused an incre...

journal_title：Biochemistry

authors： Holzman TF,Baldwin TO

更新日期：1981-09-15 00:00:00

abstract：:The enhancement of the longitudinal proton relaxation rate of solvent water protons which occurs when Mn(II) is bound to the "tight" metal ion site of unadenylylated glutamine synthetase (GS) was used to determine the binding constant of L-methionine (SR)-sulfoximine to GS-Mn(II) complexes. The binary enhancement for ...

journal_title：Biochemistry

authors： Villafranca JJ,Ash DE,Wedler FC

更新日期：1976-02-10 00:00:00

abstract：:A kinetic folding intermediate of hen lysozyme is shown to form in a nucleation/growth type of mechanism. Under native solvent conditions, a nucleated state is formed slowly during refolding (tau = 14 +/- 1 ms at 0 M GdmCl) and is rapidly converted to the folding intermediate (tau = 300 +/- 150 micros at 0 M GdmCl). U...

journal_title：Biochemistry

authors： Kiefhaber T,Bachmann A,Wildegger G,Wagner C

更新日期：1997-04-29 00:00:00

abstract：:Human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2, respectively) exhibit extensive genetic variations. It was postulated that much of this genetic variability stems from the low fidelity of the reverse transcription step. Both HIV reverse transcriptases (RTs) were shown to be particularly error-prone during...

journal_title：Biochemistry

authors： Bakhanashvili M,Hizi A

更新日期：1992-10-06 00:00:00

abstract：:The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...

journal_title：Biochemistry

authors： Yeagle PL,Alderfer JL,Albert AD

更新日期：1995-11-14 00:00:00

abstract：:Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin...

journal_title：Biochemistry

authors： Calvo FO,Keutmann HT,Bergert ER,Ryan RJ

更新日期：1986-07-01 00:00:00

abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...

journal_title：Biochemistry

authors： Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

更新日期：2013-12-03 00:00:00

abstract：:Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...

journal_title：Biochemistry

authors： Ge Y,Czekster CM,Miller OK,Botting CH,Schwarz-Linek U,Naismith JH

更新日期：2019-04-23 00:00:00