当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Electrophoretic analysis of substrate-attached proteins from normal and virus-transformed cells.

Electrophoretic analysis of substrate-attached proteins from normal and virus-transformed cells.

Abstract：

:The proteins which have been left tightly bound to the tissue culture substrate after ethylenebis (oxyethyl-enenitrilo) tetraacetic acid (EGTA)-mediated removal of normal, virus-transformed, and revertant mouse cells and which have been implicated in the substrate adhesion process have been analyzed by slab sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Three size classes of hyaluronate proteoglycans were resolved in the 5% well gel; approximately half of the protein in the substrate-attached material coelectrophoresed with these polysaccharides-so-called glycosaminoglycan-associated protein(GAP). A portion of the GAP was shown to be highly heterogeneous and displaced from the polysaccharide by preincubation with calf histone before electrophoresis. The relative proportions of the proteoglycans varied in material deposited during a variety of cellular attachment and growth conditions. The remainder of the cellular protein in substrate-attached material was resolved as several major and distinct protein bands in 8 or 20% separating gels (a limited number of distinct serum proteins have also been identified as substrate bound). Protein C0 (molecular weight 220 000) was a prominent component in the material from a variety of normal and virus-transformed cells and resembled the so-called LETS or CSP glycoprotein in several respects; protein Ca was myosin-like in several respects; protein C2 was shown to be actin; and protein C1 (molecular weight 56 000) does not appear to be tubulin. Histones were also present in most preparations of substrate-attached material, particularly at high levels in transformed cell meterial, and may result from EGTA-mediated leakiness of the cell and subsequent binding to the negatively charged polysaccharide. These substrate-attached proteins were (a) prominent in substrate-attached material from many cell types in characteristic relative proportions, (b) deposited by EGTA-subcultured cells during the first hour of attachment to fresh substrate, (c) deposited by cells growing on plastic or glass substrates (three additional) components were also prominent in glass-attached material), and (d) deposited during long-term growth on or initial attachment to substrates coated wit 3T3 substrate-attached material. Pulse-chase analyses with radioactive leucine indicated that these proteins exhibit different turn-over behaviors. These results are discussed with regard to the possible involvement of these substrate-attached proteins in the substrate adhesion process, with particular interest in the interaction of cytoskeletal microfilaments with other surface membrane components and with regard to alteration of substrate adhesion by virus transformation.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Culp LA

doi

10.1021/bi00663a028

subject

Has Abstract

pub_date

1976-09-07 00:00:00

pages

4094-104

issue

18

eissn

0006-2960

issn

1520-4995

journal_volume

15

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Isotope-edited Fourier transform infrared spectroscopy studies of calmodulin's interaction with its target peptides.

    abstract：:The ubiquitous calcium-binding protein calmodulin (CaM) regulates a wide variety of cellular events by binding to and activating many distinct target enzymes. The CaM-binding domains of most of these enzymes are contained in a contiguous stretch of amino acids with a length of approximately 20 residues. In this work, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00202a006

    authors： Zhang M,Fabian H,Mantsch HH,Vogel HJ

    更新日期：1994-09-13 00:00:00

  • Oligonucleotide site directed mutagenesis of all histidine residues within the T4 endonuclease V gene: role in enzyme-nontarget DNA binding.

    abstract：:In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00246a025

    authors： Augustine ML,Hamilton RW,Dodson ML,Lloyd RS

    更新日期：1991-08-13 00:00:00

  • Sensitivity of flavin fluorescence dynamics in neuronal nitric oxide synthase to cofactor-induced conformational changes and dimerization.

    abstract：:The fluorescence intensity of the two flavin prosthetic groups, FMN and FAD, in neuronal nitric oxide synthase (nNOS) was found to decay highly nonexponentially, being best described by four fluorescence lifetimes. This excited state heterogeneity is the result of multiple flavin quenching sites which are due to sever...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981138l

    authors： Brunner K,Tortschanoff A,Hemmens B,Andrew PJ,Mayer B,Kungl AJ

    更新日期：1998-12-15 00:00:00

  • Kinetic characterization of the carbon monoxide-acetyl-CoA (carbonyl group) exchange activity of the acetyl-CoA synthesizing CO dehydrogenase from Clostridium thermoaceticum.

    abstract：:CO dehydrogenase from Clostridium thermoaceticum is a nickel-containing enzyme that catalyzes both the reversible conversion of CO2 to CO (for incorporation into the carbonyl group of acetate) and the synthesis of acetyl-CoA from methyl corrinoid, CO, and CoASH. The latter activity is conveniently assayed by monitorin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00420a019

    authors： Raybuck SA,Bastian NR,Orme-Johnson WH,Walsh CT

    更新日期：1988-10-04 00:00:00

  • In the sense of transcription regulation by G-quadruplexes: asymmetric effects in sense and antisense strands.

    abstract：:G-Quadruplexes occupy important regulatory regions in the genome. DNA G-quadruplexes in the promoter regions and RNA quadruplexes in the UTRs (untranslated regions) have been individually studied and variously implicated at different regulatory levels of gene expression. However, the formation of G-quadruplexes in the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401451q

    authors： Agarwal T,Roy S,Kumar S,Chakraborty TK,Maiti S

    更新日期：2014-06-17 00:00:00

  • A calbindin D9k mutant with reduced calcium affinity and enhanced cooperativity. Metal ion binding, stability, and structural studies.

    abstract：:In the native calcium-binding protein calbindin D9k (M(r) 8.700; 75aa; 2 EF-hands), the backbone carbonyl oxygen of Glu60 coordinates the Ca2+ ion in the C-terminal site (site II). The carboxylate group of the same residue forms a hydrogen bond to a water molecule that constitutes a Ca2+ ligand in the N-terminal site ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00207a015

    authors： Linse S,Bylsma NR,Drakenberg T,Sellers P,Forsén S,Thulin E,Svensson LA,Zajtzeva I,Zajtsev V,Marek J

    更新日期：1994-10-18 00:00:00

  • Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy.

    abstract：:Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The corresp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00504a600

    authors： Johnson FA,Lewis SD,Shafer JA

    更新日期：1981-01-06 00:00:00

  • A General Mechanism for the Propagation of Mutational Effects in Proteins.

    abstract：:Mutations in the hydrophobic interior of proteins are generally thought to weaken the interactions only in their immediate neighborhood. This forms the basis of protein engineering-based studies of folding mechanism and function. However, mutational work on diverse proteins has shown that distant residues are thermody...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00798

    authors： Rajasekaran N,Suresh S,Gopi S,Raman K,Naganathan AN

    更新日期：2017-01-10 00:00:00

  • Post-translational modifications of aquaporin 0 (AQP0) in the normal human lens: spatial and temporal occurrence.

    abstract：:Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0496034

    authors： Ball LE,Garland DL,Crouch RK,Schey KL

    更新日期：2004-08-03 00:00:00

  • Structural elucidation of a hydrophobic box in bovine alpha-lactalbumin by NMR: nuclear Overhauser effects.

    abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00346a035

    authors： Koga K,Berliner LJ

    更新日期：1985-12-03 00:00:00

  • Mechanistic investigations of a ribozyme derived from the Tetrahymena group I intron: insights into catalysis and the second step of self-splicing.

    abstract：:Self-splicing of Tetrahymena pre-rRNA proceeds in two consecutive phosphoryl transesterification steps. One major difference between these steps is that in the first an exogenous guanosine (G) binds to the active site, while in the second the 3'-terminal G414 residue of the intron binds. The first step has been extens...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9527653

    authors： Mei R,Herschlag D

    更新日期：1996-05-07 00:00:00

  • Deuteron nuclear magnetic resonance studies of phase equilibria in a lecithin-water system.

    abstract：:Water deuteron NMR spectra have been studied for the system dipalmitoyllecithin (DPL)-heavy water (D2O) at different compositions and temperatures. From an analysis of the spectra in terms of quadrupole splittings, a phase diagram has been constructed for the temperature range 25-60 degrees C and the composition range...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00645a014

    authors： Ulmius J,Wennerström H,Lindblom G,Arvidson G

    更新日期：1977-12-27 00:00:00

  • Synthesis of 3'-C-methyladenosine and 3'-C-methyluridine diphosphates and their interaction with the ribonucleoside diphosphate reductase from Corynebacterium nephridii.

    abstract：:Two nucleoside diphosphate analogs, 3'-C-methyl-ADP and 3'-C-methyl-UDP, have been tested as substrate and/or allosteric effectors using the adenosylcobalamin-dependent ribonucleoside diphosphate reductase of Corynebacterium nephridii. Neither analog was a substrate for the reductase. However, they did function as all...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00160a035

    authors： Ong SP,Nelson LS,Hogenkamp HP

    更新日期：1992-11-17 00:00:00

  • Retinoschisin (RS1) interacts with negatively charged lipid bilayers in the presence of Ca2+: an atomic force microscopy study.

    abstract：:Retinoschisin (RS1) is a retina-specific secreted protein encoding a conserved discoidin domain sequence. As an adhesion molecule, RS1 preserves the retinal cell architecture and promotes visual signal transduction. In young males, loss-of-function mutations in the X-linked retinoschisis gene (RS1) cause X-linked reti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1007029

    authors： Kotova S,Vijayasarathy C,Dimitriadis EK,Ikonomou L,Jaffe H,Sieving PA

    更新日期：2010-08-24 00:00:00

  • Mechanism of glutamate transport in Escherichia coli B. 1. Proton-dependent and sodium ion dependent binding of glutamate to a glutamate carrier in the cytoplasmic membrane.

    abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00277a033

    authors： Fujimura T,Yamato I,Anraku Y

    更新日期：1983-04-12 00:00:00

  • Development of a neutralizing antibody specific for the active form of matrix metalloproteinase-13.

    abstract：:Matrix metalloproteinase-13 (MMP-13) is important in the pathology of osteoarthritis (OA). Although MMP-13 is considered a therapeutic target for OA, it is unclear how MMP-13 activity is regulated by the system that comprises various proteinases and their inhibitors. MMP-13 neutralizing antibodies could be a useful to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301228d

    authors： Naito S,Takahashi T,Onoda J,Yamauchi A,Kawai T,Kishino J,Yamane S,Fujii I,Fukui N,Numata Y

    更新日期：2012-11-06 00:00:00

  • Tryptophan 697 modulates hydride and interflavin electron transfer in human methionine synthase reductase.

    abstract：:Human methionine synthase reductase (MSR), a diflavin oxidoreductase, plays a vital role in methionine and folate metabolism by sustaining methionine synthase (MS) activity. MSR catalyzes the oxidation of NADPH and shuttles electrons via its FAD and FMN cofactors to inactive MS-cob(II)alamin. A conserved aromatic resi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2012228

    authors： Meints CE,Gustafsson FS,Scrutton NS,Wolthers KR

    更新日期：2011-12-27 00:00:00

  • A single mutation in cytochrome P450 BM3 changes substrate orientation in a catalytic intermediate and the regiospecificity of hydroxylation.

    abstract：:Phenylalanine 87 of Bacillus megaterium cytochrome P450 BM3, a residue close to the heme in the substrate binding pocket, has been replaced by alanine by site-directed mutagenesis. The substitution had no effect on the rate of hydroxylation of laurate and increased the affinity for laurate of both the intact enzyme an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962826c

    authors： Oliver CF,Modi S,Sutcliffe MJ,Primrose WU,Lian LY,Roberts GC

    更新日期：1997-02-18 00:00:00

  • A typical preparation of Francisella tularensis O-antigen yields a mixture of three types of saccharides.

    abstract：:Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low infectious dose, high mortality rate, and the threat of its large-scale dissemination in weaponized form, development of vaccines and immunotherapeutics against Ft is essential. Ft l...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201450v

    authors： Wang Q,Shi X,Leymarie N,Madico G,Sharon J,Costello CE,Zaia J

    更新日期：2011-12-20 00:00:00

  • Abasic frameshift in DNA. Solution conformation determined by proton NMR and molecular mechanics calculations.

    abstract：:We have determined the three-dimensional structure of a non-self-complementary oligodeoxynucleotide duplex that contains a model abasic site. The duplex contains six GC base pairs plus the abasic site at the center of one strand and corresponds to an abasic frameshift. Two-dimensional NMR studies on the nonexchangeabl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00431a009

    authors： Cuniasse P,Sowers LC,Eritja R,Kaplan B,Goodman MF,Cognet JA,Le Bret M,Guschlbauer W,Fazakerley GV

    更新日期：1989-03-07 00:00:00

  • Phosphinothricin-tripeptide synthetases from Streptomyces viridochromogenes.

    abstract：:Phosphinothricyl-alanyl-alanine (Pt tripeptide (Ptt), bialaphos) is a metabolite produced by Streptomyces viridochromogenes and Streptomyces hygroscopicus. It contains the unique phosphinoamino acid phosphinothricin (Pt), which after cleavage from Ptt is active as an inhibitor of glutamine synthetase. We have isolated...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9719410

    authors： Grammel N,Schwartz D,Wohlleben W,Keller U

    更新日期：1998-02-10 00:00:00

  • Investigation of the shape and size of myosin subfragment 1 using small-angle X-ray scattering.

    abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00605a009

    authors： Kretzschmar KM,Mendelson RA,Morales MF

    更新日期：1978-06-13 00:00:00

  • Effects of aluminum and other cations on the structure of brain and liver chromatin.

    abstract：:The reactivity of aluminum and several other divalent and trivalent metallic cations toward chromatin from rat brain and liver has been investigated. Two criteria are used to determine the relative reactivity of these cations toward chromatin. The first involves the ability of the ions to compact the chromatin fibers ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a043

    authors： Walker PR,LeBlanc J,Sikorska M

    更新日期：1989-05-02 00:00:00

  • Chemical modification of mitochondrial transhydrogenase: evidence for two classes of sulfhydryl groups.

    abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00615a015

    authors： Earle SR,O'Neal SG,Fisher RR

    更新日期：1978-10-31 00:00:00

  • Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4.

    abstract：:Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covalent complex containing ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00248a014

    authors： Graves KL,Hardy LW

    更新日期：1994-11-08 00:00:00

  • SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study.

    abstract：:SecA-lipid interactions are believed to be important for the translocation of precursor proteins across the inner membrane of Escherichia coli [Lill, R., Dowhan, W., & Wickner, W. (1990) Cell 60, 271-280]. SecA insertion into the phospholipid bilayer could a role in this process. We investigated this possibility by st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00119a021

    authors： Breukink E,Demel RA,de Korte-Kool G,de Kruijff B

    更新日期：1992-02-04 00:00:00

  • Specific binding of ATP to extracellular sites on Torpedo acetylcholine receptor.

    abstract：:The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00080a002

    authors： Carlson BJ,Raftery MA

    更新日期：1993-07-27 00:00:00

  • Membrane glycopeptides from virus-transformed hamster fibroblasts and the normal counterpart.

    abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00579a015

    authors： Glick MC

    更新日期：1979-06-12 00:00:00

  • Long-range structural and dynamical changes induced by cofactor binding in DNA methyltransferase M.HhaI.

    abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602662e

    authors： Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

    更新日期：2007-06-19 00:00:00

  • Multiple conformations of NAD and NADH when bound to human cytosolic and mitochondrial aldehyde dehydrogenase.

    abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi012197t

    authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

    更新日期：2002-06-04 00:00:00