Abstract:
:The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with increasing concentration of 8-azido-ATP and the inhibition of photolabeling by ATP. Protection studies suggest that the binding sites for the photolabel are unique and are not associated with the cholinergic ligand binding sites.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Carlson BJ,Raftery MAdoi
10.1021/bi00080a002subject
Has Abstractpub_date
1993-07-27 00:00:00pages
7329-33issue
29eissn
0006-2960issn
1520-4995journal_volume
32pub_type
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