Abstract：
:It is generally accepted that electron transfer in bacterial photosynthesis is driven by the first singlet excited state of a special pair of bacteriochlorophylls (P*). We have examined the first steps of electron transfer in a mutant of the Rhodobacter sphaeroides reaction center in which charge separation from P* is dramatically slowed down. The results provide for the first time clear evidence that excitation of the monomeric bacteriochlorophyll in the active branch of the reaction center (B(A)) drives ultrafast transmembrane electron transfer without the involvement of P*, demonstrating a new and efficient mechanism for solar energy transduction in photosynthesis. The most abundant charge-separated intermediate state probably is P+B(A)-, which is formed within 200 fs from B(A)* and decays with a lifetime of 6.5 ps into P+H(A)-. We also see evidence for the involvement of a B(A)+H(A)- state in the alternative pathway.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Van Brederode ME,Jones MR,Van Mourik F,Van Stokkum IH,Van Grondelle Rdoi
10.1021/bi9703756subject
Has Abstractpub_date
1997-06-10 00:00:00pages
6855-61issue
23eissn
0006-2960issn
1520-4995pii
bi9703756journal_volume
36pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:NMR spectroscopy and simulated annealing calculations have been used to determine the three-dimensional structure of RK-1, an antimicrobial peptide from rabbit kidney recently discovered from homology screening based on the distinctive physicochemical properties of the corticostatins/defensins. RK-1 consists of 32 res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000457l
更新日期：2000-12-26 00:00:00
abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060421e
更新日期：2006-09-26 00:00:00
abstract：:Processing bodies (P-bodies) are cytoplasmic ribonucleoprotein (RNP) granules primarily composed of translationally repressed mRNAs and proteins related to mRNA decay, suggesting roles in post-transcriptional regulation. P-bodies are conserved in eukaryotic cells and exhibit properties of liquid droplets. However, the...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.7b01162
更新日期：2018-05-01 00:00:00
abstract：:Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300113x
更新日期：2012-07-31 00:00:00
abstract：:There is much debate whether the fatty acid substrate of lipoxygenase binds "carboxylate-end first" or "methyl-end first" in the active site of soybean lipoxygenase-1 (sLO-1). To address this issue, we investigated the sLO-1 mutants Trp500Leu, Trp500Phe, Lys260Leu, and Arg707Leu with steady-state and stopped-flow kine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0489098
更新日期：2004-10-19 00:00:00
abstract：:Segregation of mRNAs in the cytoplasm of polar cells has been demonstrated for proteins involved in Xenopus and Drosophila oogenesis, and for some proteins in somatic cells. It is assumed that vectorial transport of the messages is generally responsible for this localization. The mRNA encoding the basic protein of cen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9800247
更新日期：1998-05-12 00:00:00
abstract：:Previous studies of P-glycoprotein have demonstrated that its function can be modulated by phosphorylation. In the present study, inhibition of protein kinase C with calphostin C or stauroporine or prolonged treatment with the phorbol ester TPA decreased phosphorylation of P-glycoprotein, and impaired transport of vin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00086a022
更新日期：1993-09-07 00:00:00
abstract：:Cytochrome P450 3A4 is a drug-metabolizing enzyme of extraordinarily broad substrate specificity. This quality imparts upon the enzyme special importance in understanding its determinants of activity and substrate recognition. Limited successes in P450 3A4 active-site structure studies have been achieved by use of mec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049231z
更新日期：2005-02-15 00:00:00
abstract：:The binding of human Glu- and Lys-plasminogens to intact fibrin clots, to lysine-Sepharose, and to fibrin cleaved by plasmin was quantitatively characterized. On intact fibrin clots, there was one strong binding site for Glu-plasminogen with a dissociation constant, Kd, of 25 microM and one strong binding site for Lys...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00334a031
更新日期：1985-06-18 00:00:00
abstract：:RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501530u
更新日期：2015-02-17 00:00:00
abstract：:To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001342k
更新日期：2000-10-24 00:00:00
abstract：:Time-resolved infrared (TRIR) techniques have been employed to study the reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome c oxidase (CcO). The ligation dynamics immediately following photodissociation have been investigated using picosecond TRIR spectroscopy and linear dichroism. The r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00168a015
更新日期：1994-01-18 00:00:00
abstract：:Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00350
更新日期：2017-07-25 00:00:00
abstract：:c-Myc is a member of a family of sequence specific-DNA binding proteins that are thought to regulate the transcription of genes involved in normal cell growth, differentiation, and apoptosis. In order to understand how human c-myc functions as a transcription factor, we have studied the mechanism of action and structu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960793v
更新日期：1996-07-23 00:00:00
abstract：:Three mutants, Asp55-Gly, Tyr306-->Phe, and Asp309-->Asn, of Aspergillus awamori glucoamylase (identical to Aspergillus niger glucoamylase) were constructed to elucidate the roles of two conserved regions within fungal glucoamylases. Kinetic studies indicate that both of these regions are closely associated with activ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00055a017
更新日期：1993-02-02 00:00:00
abstract：:ATP, its nonhydrolyzable analogue, AMP-PNP, and albumin were found to promote the dissociation of rhodopsin kinase from rod outer segments (ROS) containing photoactivated-rhodopsin (Rho*). These features were embodied in a protocol for the recovery of rhodopsin kinase from incubations containing ROS which had been sub...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952480q
更新日期：1996-05-14 00:00:00
abstract：:De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102033a
更新日期：2011-05-31 00:00:00
abstract：:Globotriaosylceramide [(Gal alpha 1-4Gal beta 1-4Glc-ceramide (Gb3)] was separated from human kidney, and the fatty acid composition was determined. Semisynthetic Gb3 molecular species of corresponding fatty acid chain length were prepared and compared for verotoxin (VT) binding affinity by TLC overlay, and a quantita...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00120a011
更新日期：1992-02-11 00:00:00
abstract：:Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5' terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) ribonucleic acid (RNA) to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat gern 80S ribosomes convey partial p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00551a044
更新日期：1980-05-13 00:00:00
abstract：:Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00226a012
更新日期：1991-03-26 00:00:00
abstract：:Escherichia coli 3-methyladenine DNA glycosylase II (AlkA), an adaptive response glycosylase with a broad substrate range, initiates base excision repair by flipping a lesion out of the DNA duplex and hydrolyzing the N-glycosidic bond. We used transient and steady state kinetics to determine the minimal mechanism for ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501356x
更新日期：2015-01-27 00:00:00
abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0477034
更新日期：2005-04-26 00:00:00
abstract：:The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201603f
更新日期：2012-01-10 00:00:00
abstract：:Mutagenesis of Escherichia coli manganese superoxide dismutase (MnSD) demonstrates involvement of the strictly conserved gateway tyrosine (Y34) in exogenous ligand interactions. Conservative replacement of this residue by phenylalanine (Y34F) affects the pH sensitivity of the active-site metal ion and perturbs ligand ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9704212
更新日期：1997-07-22 00:00:00
abstract：:Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00058a013
更新日期：1993-02-23 00:00:00
abstract：:The bacterial alpha-lytic protease (alphaLP) is synthesized as a precursor containing a large N-terminal pro region (Pro) transiently required for correct folding of the protease [Silen, J. L., and Agard, D. A. (1989) Nature 341, 462-464]. Upon folding, the precursor is autocatalyticly cleaved to yield a tight-binding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982165e
更新日期：1999-04-13 00:00:00
abstract：:The heat production for the reaction steps of ATP hydrolysis by heavy meromyosin has been reexamined by using a reaction-type calorimeter. So far, the values reported for the heat production have varied substantially among research groups. The present results obtained in 0.3 M KCl, 10 mM MgCl2, and 20 mM Tris-HCl at p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00518a037
更新日期：1981-07-21 00:00:00
abstract：:Magainin and PGLa are 23- and 21-residue peptides isolated from the skin of the African clawed frog Xenopus laevis. They protect the frog from infection and exhibit a broad-spectrum antimicrobial activity in vitro. The mechanism of this activity involves the interaction of magainin with microbial membranes. We have me...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00470a031
更新日期：1990-05-08 00:00:00
abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00632a012
更新日期：1977-06-28 00:00:00
abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00406a049
更新日期：1988-03-22 00:00:00
